• Korean Journal of Veterinary Research
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• v.41 no.4
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• pp.535-542
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• 2001

A multiplex PCR technique was developed for detecting specifically each Mycobacterium bovis, M. tuberculosis, M. avium and M. avium subsp, paratuberculosis, respectively, using clinical samples of field cattle. To apply this novel technique to clinical specimens, blood sample was obtained from live cows comprising 11 intradermal tuberculin test (ITT)-positive and 17 ITT-negative and tested by multiplex PCR. Positive results were obtained from 15 cows by the multiplex PCR, showing that 4 (23.5%) of the 17 ITT-negative cows were multiplex PCR positive. The multiplex PCR results also showed that among the 15 positive cows, 7 (46.7%) were infected with M. bovis, 1 (6.7%) with M. tuberculosis and 7 (46.7%) with M. avium. The sensitivity and specificity of multiplex PCR in comparison with those of ITT were 100% and 76.5%. The correlation between the multiplex PCR and ITT assays with blood samples was considered excellent, 85.7% agreement and ${\kappa}=0.72$. The results obtained, using reference mycobacterial strains and typed clinical samples, show that the multiplex PCR method may be a rapid, sensitive, and specific tool for the differential identification of various mycobacterial strains in a single-step assay. Therefore, multiplex PCR assay is a useful tool for early diagnosis of tuberculosis in live cattle and to identify the species or complex of mycobacterium from clinical samples.

• The Korean Journal of Mycology
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• v.28 no.1
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• pp.55-59
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• 2000

This study was carried out to investigate the antioxidative and chemopreventive effects of the extracts from Polyozellus multiplex, an edible mushroom through in vitro and in vivo assay. Polyozellus multiplex fractions were assayed for its antioxidative effect with colony formation assay. Polyozellus multiplex methanol extract and water fraction showed protective effects against the cytotoxicity of $H_2O_2$. The modifying effects of Polyozellus multiplex methanol extract and water fraction on the induction of carcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were investigated in Wistar rats. The GSH content was decreased by MNNG treatment but was increased by adding Polyozellus multiplex water fractions. Also the activity of glutathione S-transferase and the superoxide dismutase levels were increased by the treatment of Polyozellus multiplex water fractions more than with MNNG alone. In addition to the Polyozellus multiplex water fraction increased the p53 expression as compared with the value of MNNG alone.

• Korean System Dynamics Review
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• v.10 no.2
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• pp.5-27
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• 2009

Multiplex is a representative culture facility of citizens. Therefore, a lot of researches and investment on multiplex are carried out to improve benefits of service suppliers and users. Especially, focused on main services of a theatre such as ticket booking and issuing within multiplex, examination of tickets, admission information of movie screens and screening information inquiry, improvement activities are carried out. However, it is not enough to evaluate on what efficiency the above efforts have in the viewpoint of customer benefits and business. Therefore, this study analyzed value and limit of the newest service of multiplex applying the existing ubiquitous concept(u-multiplex service), and proposed a model and a plan for improving the existing services. The study interviewed with specialists in the related field and applied workshop-shape group interview to 110 university students and simulated service models. The contribution of the study is to analyze the value and limit of the existing multiplex service objectively, and to propose a new service model and plan to improve its limitation. In the future, the study plans to research on service models by extending space and functional roles of multiplex to the whole subsidiary facilities including movie screens.

• Journal of Information Technology Applications and Management
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• v.26 no.6
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• pp.139-157
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• 2019

This study is intended for demonstrating the impacts of different factors on the formation of online consumer engagement behavior of young Chinese moviegoer in Korea. Based on Value-Exchange Model [Itani et al., 2019], we build research model to reveal the relationships among perceived enjoyment, perceived movie information value, multiplex-audience relationship quality, multiplex usage satisfaction, and online consumer engagement through experiments on valid data we collected from 186 participants who had lived in Korea and experienced the multiplex pages of top 3 movie theaters, where Smart PLS 3.0 is the tool used for statistical analysis. The experimental results show that both perceived enjoyment and perceived movie information value positively correlate to multiplex-audience relationship quality, and multiplex audience relationship quality significantly influences multiplex usage satisfaction and online consumer engagement. In addition, it is found that relationship quality plays the role of mediator between perceived enjoyment and satisfaction. The findings from this study offer both academic and managerial implications for movie distributors who are interested in developing potential Chinese consumer market in Korea.

• Journal of Practical Agriculture & Fisheries Research
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• v.5 no.1
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• pp.57-63
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• 2003

The bovine rotavirus(BRV), bovine coronavirus(BCV) and bovine viral diarrhea virus(BVDV) are main viruses of bovine viral diarrhea disease. These viruses could be rapidly amplified by the reverse transcriptase polymerase chain reaction(RT-PCR). This study was conducted to develop rapid and accurate diagnostic methods of these viral diseases by multiplex RT-PCR. Specific primers were designed based on the sequences reported by Chang KO et. al. (1997) and Schroeder BA, et. al. (1990), RNA were prepared from the cultured viruses, first-stranded DNAs were synthesised by reverse transcriptase. PCR were conducted to amplify specific regions of the viruses by multiplex. Three bands such as 1,062bp for BRV, 458bp for BCV, and 300bp for BVDV were successfully produced by multiplex RT-PCR. In conclusion, this result suggested that these viruses could be diagnosed rapidly and accurately by multiplex RT-PCR.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2005.11a
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• pp.187-190
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• 2005

By the rapid technological advance and social needs, demand of Multi Function Facility is on the increase. The multiplex is an alternative of them. In case of fire, special function of facility nay result in terrible accident. Therefore, literature and regulations survey on the multiplex were enforced, Multiplex facilities in Daejeon City were surveyed, and reformation of regulation and structural system were suggested in this study.

• Korean Journal of Microbiology
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• v.48 no.4
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• pp.309-313
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• 2012

The purpose of this study is to simultaneously detect and identify specific genus of Lactobacillus distributed in vagina of healthy women in their twenties by using multiplex primer. Vaginal fluid samples were taken from 166 women who were healthy and did not have vaginosis symptoms caused by bacterial infection. Six species of lactic acid bacteria belong to Lactobacillus genus that are known to inhabit in healthy vagina were selected to make a species-specific multiplex primer. Multiplex primer I was specified to selectively detect L. iners, L. crispatus, L. gasseri and multiplex primer II was specified to selectively detect L. acidophilus, L. jensenii, L. fermentum. L. crispatus (77%) was most frequently detected, and L. acidophilus (57%) and L. jensenii (57%) were relatively higher than others. Although the proportion of L. iners (59%) was a little higher than those of L. acidophilus and L. jensenii, this should be further validated in healthy women's vagina to be sure since they often appear in women having bacterial vaginosis and/or during the antibiotic therapy. Conclusively, the multiplex PCR technique using the species-specific primer could a useful tool to predict variation of vaginal health condition and process of recovery from vaginal disease.

• Food Science of Animal Resources
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• v.34 no.5
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• pp.717-723
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• 2014

A rapid and specific PCR assay for the simultaneous detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. Multiplex PCR developed in this study produced only actA, fliC, hbl, invA, ileS amplicons, but did not produce any non-specific amplicon. The primer sets successfully amplified the target genes in the multiplex PCR without any non-specific or additional bands on the other strains. The multiplex PCR assays also amplified some target genes from five pathogens, and multiplex amplification was obtained from as little as 1 pg of DNA. According to the results from the sensitivity evaluation, the multiplex PCR developed in this study detected 10 cells/mL of the pathogens inoculated in milk samples, respectively. The results suggested that multiplex PCR was an effective assay demonstrating high specificity for the simultaneous detection of five target pathogens in food system.

• Korean Journal of Veterinary Research
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• v.43 no.2
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• pp.231-237
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• 2003

Listeria (L.) monocytogenes in samples could not be detected occasioally by faster growth of other Listeria spp. especially L. innocua. The aim of this study was to develop the differential media and multiplex polymerase chain reaction (PCR) assays for the rapid detection of L. monocytogenes. L. monocytogenes colonies were characterized by their ${\beta}$-hemolysis with fluorescence under 366 nm UV light on the Listeria hemolysis agar (LHA). L. innocua, a species commonly present in foods, did not produce ${\beta}$-hemolysis on LHA. Therefore, one or more colonies of L. monocytogenes were easily distinguished from large populations of L. innocua. The multiplex PCR assays were developed to distinguish from L. monocytogenes and other Listeria spp. with two pairs of primers. The primers were designed in 16S rRNA and listeriolysin O gene for specific amplification of all members of the genus Listeria and L. monocytogenes, respectively. The multiplex PCR assays produced 560 and 938 bp products in L. monocytogenes; only 938 bp products in the genus Listeria. The multiplex PCR assays could detect as little as 50 pg of L monocytogenes DNA. These results indicated that the differential media and multiplex PCR assays might be useful diagnostic tools for the rapid detection of L. monocytogenes.

• Korean Institute of Interior Design Journal
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• v.27 no.1
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• pp.39-47
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• 2018

This study is intended to suggest an improvement scheme of activating container multiplex cultural space through an analysis of container architecture characteristics and a survey of user satisfaction, focusing on container multiplex cultural space located in Seoul city. Hence, the elements of multiplex cultural space and the characteristics of container architecture were deduced and analyzed through precedent study and literature review relating to multiplex cultural space and container architecture, and then an analysis framework was established with items to measure the user satisfaction. Based on this, a questionnaire was created, and users were surveyed through on-site visit. The results of analyzing the container multiplex cultural space users' satisfaction are as follows. First, in order to increase user satisfaction, it is necessary to highlight visual element. Second, there is necessity for distinct space configuration, such as connection, stack and extension by utilizing the fixed specifications, the characteristics of container. Third, there is necessity for extended space where people feel a sense of openness. Fourth, it should be improved so that air is well circulated by installing glass, operable window, etc. In further research, it is thought necessary to make a study of commercial space and facilities inside university besides multiplex cultural space.