• 국제지역연구
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• 제18권3호
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• pp.205-229
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• 2014

글로벌 생산 네트워크는 다국적기업들이 전략적으로 자사의 가치사슬을 단계별로 분리하여 다양한 지역으로 이전시키면서 나타나기 시작하였다. 글로벌 생산 네트워크는 자재, 부품, 소재, 그리고 완제품의 빈번한 운송을 수반하고, 신속하고 효율적인 운용을 요구하기 때문에 역외보다는 통합된 지역 내에서 더욱 발달하였다. 여러 해의 자료를 심층 분석한 결과, 해당 지역 내에서 고부가가치 상품의 생산에 비교우위를 지닌 선진국들은 ICT 부품과 소재의 생산에 특화하며 높은 수출 현시비교우위지수를 보였다. 반면에 노동집약적인 조립 공정에 비교우위를 가진 개도국들은 높은 수입 현시비교우위지수를 보여주었다. 일부 개도국들은 글로벌 생산 네트워크 참여를 통해 꾸준히 기술역량을 제고하면서 산업구조 고도화 발전을 이룰 수 있었음 또한 자료 분석 결과 알 수 있었다. 동아시아 국가들은 다양한 요인들로 인해 EU나 NAFTA 지역 국가들에 비해 글로벌 생산 네트워크에 많이 참여하였는데, 해당 국가들의 ICT 분야 기술발전 속도 및 수출의 빠른 증가세가 보여주듯이 동아시아 국가들은 글로벌 생산 네트워크 참여로 크게 수혜하였다. 그러나 이는 결과적으로 ICT 부품과 소재 산업에 있어서 글로벌 생산 네트워크의 지역별 구축 정도에 상당한 불균형을 야기하였음을 확인할 수 있었다.

• 융합정보논문지
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• 제9권1호
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• pp.86-91
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• 2019

2008년 금융위기 사태가 발생한 이후 금융 시스템의 중앙집권의 위험성을 인지하면서 시작된 것이 블록체인이다. 2008년 시작점으로부터 지금까지 블록체인은 비트코인이라는 블록체인 기술 기반 암호화폐란 엄청난 혁신을 시작으로 많은 관심과 기술의 발전이 이루어지고 있는 상황이다. 하지만 이 또한 시간이 지나면서 취약점이 발견되었고 피해 사례 또한 자주 일어나고 있다. 그러나 현재 21세기 4차 산업에 있어 핵심 기술이며 앞으로도 많은 발전과 이를 기반으로 한 기술들이 다수 출현할 것으로 예상된다. 따라서 본 논문은 암호화폐 거래에서의 취약점을 분석하고 새로운 거래 프로토콜을 제안한다. 제안하는 거래 프로토콜은 거래가 끝나는 시점에서 한 번 더 인증을 하게 되고 이러한 인증으로 거래가 성사되는 방식이다. 거래 취약점에 대한 내부적 보안이 아닌 거래 과정에서 사용자들 간의 보안요소를 추가하여 대응방안을 제안한다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권6호
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• pp.757-766
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• 2019

Objective: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. Results: The results showed total of 1,086 known miRNAs and 40 new candidate miRNAs were detected in prenatal and postnatal skeletal muscle of sheep. In addition, 345 miRNAs (151 up-regulated, 94 down-regulated) were differentially expressed. Moreover, miRanda software was performed to predict targeted genes of miRNAs, resulting in a total of 2,833 predicted targets, especially miR-381 which targeted multiple muscle-related mRNAs. Furthermore, GO and KEGG pathway analysis confirmed that targeted genes of miRNAs were involved in development of skeletal muscles. Conclusion: This study supplements the miRNA database of sheep, which provides valuable information for further study of the biological function of miRNAs in sheep skeletal muscle.

• Laboraroty Animal Research
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• 제34권4호
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• pp.232-238
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• 2018

Animal models have been used to elucidate the pathophysiology of varying diseases and to provide insight into potential targets for therapeutic intervention. Although alternatives to animal testing have been proposed to help overcome potential drawbacks related to animal experiments and avoid ethical issues, their use remains vital for the testing of new drug candidates and to identify the most effective strategies for therapeutic intervention. Particularly, the study of metabolic diseases requires the use of animal models to monitor whole-body physiology. In line with this, the National Institute of Food and Drug Safety Evaluation (NIFDS) in Korea has established their own animal strains to help evaluate both efficacy and safety during new drug development. The objective of this study was to characterize the response of C57BL/6NKorl mice from the NIFDS compared with that of other mice originating from the USA and Japan in a chemical-induced diabetic condition. Multiple low-dose treatments with streptozotocin were used to generate a type-1 diabetic animal model which is closely linked to the known clinical pathology of this disease. There were no significantly different responses observed between the varying streptozotocin-induced type-1 diabetic models tested in this study. When comparing control and diabetic mice, increases in liver weight and disturbances in serum amino acids levels of diabetic mice were most remarkable. Although the relationship between type-1 diabetes and BCAA has not been elucidated in this study, the results, which reveal a characteristic increase in diabetic mice of all origins are considered worthy of further study.

• 동물자원연구
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• 제28권2호
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• pp.78-96
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• 2017

구제역은 발생 시 전염이 쉽게 일어나며 심각한 경제적 피해를 일으키는 질병이다. 구제역의 방역정책은 발견 직후 빠른 살처분이 최선책이나, 전파 속도나 상황 등에 따라 타지역 백신 접종 등의 방법을 시행할 수도 있다. 이러한 방법을 적용하기 위해서는 구제역을 빠르고 정확하게 진단할 필요성이 있다. 개발된 진단법들은 구제역의 확진, 혈청형의 동정, 백신 접종 후 항체의 생성 확인 등에 사용된다. 많은 진단법들이 개발되었지만 아직은 빠른 시간 내에 검출이 가능하며 동시에 정확성도 가진 방법이 드물다. 그렇기에 기존의 방법들을 개선시킨 새로운 진단법이 필요하다. 현재는 대부분 혈청학적 진단법인 ELISA에 의존하거나 분자 유전학적 기술인 PCR을 사용한다. 가장 최근 기술은 그 둘을 합치는 방법으로, 어떻게 하면 더 신속하고 저비용이면서, 민감하고 정확한 방법이 될 수 있을지 연구가 진행되고 있다.

• 대한건축학회논문집:계획계
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• 제36권3호
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• pp.69-78
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• 2020

This study is designed to find out the role and relation of the Nemok-dori support members in order to clearly specify the structure of the Dapogye architecture which adopts the Nemok-dori. The dori at the eaves in the Dapogye architecture was expanded to include Oemok-dori, Jusim-dori and Nemok-dori in cooperation with the bracket. The Oemok-dori is used to lengthen the eaves and the Jusim-dori makes the reasonable transfer of loads while the Namok-dori contributes to the stability of the building through the balancing of the bracket. Nemok-dori is located higher than Oemok-dori or Jusim-dori, depending on the slope of Jangyeon and the distance of Chulmok. But as it is not directly supported by bracket or beam, it needs an independent support. The vertical support of Nemok-dori is selectively involved with Cheomcha, lower Jangyeo, support Wall and upper Jangyeo. As the time went by, the vertical height increased, thus making the omitted members less. The horizontal support of Nemok-dori is made in various ways. There are also special cases such as the Nemok-dori □ type chain structure for small rectangular buildings, the 'Nemok-dori = Jung-dori' type for the buildings having small sides and the 'Nemok-dori = Meongechangbang' type appearing on the lower part of the Bankanmulrim type middle height buildings. The horizontal support of Nemok-dori usually uses either the support of the Toeryang type member, the support of beam directed Hwaban or the support through overlaying of internal Jegong. As the time went by and the number and distance of Chulmok increased, the multiple support structures acted together rather than as one support structure. Nemok-dori got stabilized as it has both the vertical and horizontal supports and this support structure also has he role of decoration.

• The Plant Pathology Journal
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• 제37권2호
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• pp.87-98
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• 2021

To establish an infection, fungal pathogens must recognize diverse signals from host surfaces. The rice blast fungus, Magnaporthe oryzae, is one of the best models studying host-pathogen interactions. This fungus recognizes physical or chemical signals from the host surfaces and initiates the development of an infection structure called appressorium. Here, we found that protein MoAfo1(appressorium formation, MGG_10422) was involved in sensing signal molecules such as cutin monomers and long chain primary alcohols required for appressorium formation. The knockout mutant (ΔMoafo1) formed a few abnormal appressoria on the onion and rice sheath surfaces. However, it produced normal appressoria on the surface of rice leaves. MoAfo1 localized to the membranes of the cytoplasm and vacuole-like organelles in conidia and appressoria. Additionally, the ΔMoafo1 mutant showed defects in appressorium morphology, appressorium penetration, invasive growth, and pathogenicity. These multiple defects might be partially due to failure to respond properly to oxidative stress. These findings broaden our understanding of the fungal mechanisms at play in the recognition of the host surface during rice blast infection.

• Journal of Chest Surgery
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• 제54권1호
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• pp.9-16
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• 2021

Background: The deposition of monomeric C-reactive protein (mCRP) in the myocardium aggravates ischemia-reperfusion injury (IRI) and myocardial infarction. Ischemic preconditioning (IPC) is known to protect the myocardium against IRI. Methods: We evaluated the effects of IPC on myocardium upon which mCRP had been deposited due to IRI in a rat model. Myocardial IRI was induced via ligation of the coronary artery. Direct IPC was applied prior to IRI using multiple short direct occlusions of the coronary artery. CRP was infused intravenously after IRI. The study included sham (n=3), IRI-only (n=5), IRI+CRP (n=9), and IPC+IRI+CRP (n=6) groups. The infarcted area and the area at risk were assessed using Evans blue and 2,3,5-triphenyltetrazolium staining. Additionally, mCRP immunostaining and interleukin-6 (IL-6) mRNA reverse transcription-polymerase chain reaction were performed. Results: In the IRI+CRP group, the infarcted area and the area of mCRP deposition were greater, and the level of IL-6 mRNA expression was higher, than in the IRI-only group. However, in the IPC+IRI+CRP group relative to the IRI+CRP group, the relative areas of infarction (20% vs. 34%, respectively; p=0.079) and mCRP myocardial deposition (21% vs. 44%, respectively; p=0.026) were lower and IL-6 mRNA expression was higher (fold change: 407 vs. 326, respectively; p=0.376), although the difference in IL-6 mRNA expression was not statistically significant. Conclusion: IPC was associated with significantly decreased deposition of mCRP and with increased expression of IL-6 in myocardium damaged by IRI. The net cardioprotective effect of decreased mCRP deposition and increased IL-6 levels should be clarified in a further study.

• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1508-1518
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• 2021

Hsa_circ_0001947 is associated with multiple cancers, but its function in non-small cell lung cancer (NSCLC) is ambiguous and needs further research. The targeting relationship among circ_0001947, miR-661, and downstream of tyrosine kinase 7 (DOK7) was predicted by database and further verified by dual-luciferase reporter assay, while their expressions in cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection, cell biological behaviors and expressions of miRNAs, miR-661 and DOK7 were determined by cell function experiments and qRT-PCR, respectively. Circ_0001947 was low-expressed in NSCLC tissues and cells. Circ_0001947 knockdown intensified cell viability and proliferation, induced cell cycle arrest at S phase, suppressed apoptosis and evidently enhanced miR-510, miR-587, miR-661 and miR-942 levels, while circ_0001947 overexpression did the opposite. MiR-661 was a target gene of circ_0001947 that participated in the regulation of circ_0001947 on cell biological behaviors. Furthermore, DOK7, the target gene of miR-661, partly participated in the regulation of miR-661 on cell viability. Hsa_circ_0001947 acts as a sponge of miR-661 to repress NSCLC development by elevating the expression of DOK7.

• Journal of Veterinary Science
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• 제23권4호
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• pp.56.1-56.17
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• 2022

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were "protein processing in endoplasmic reticulum," "retinol metabolism," and "glycine, serine, and threonine metabolism." Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.