• 한국의상디자인학회지
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• 제23권2호
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• pp.115-132
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• 2021

The purpose of this study was to suggest multiple directions of fashion design by producing differentiated athleisure fashion designs with functional materials based on existing sportswear. A design utilizing handcraft skill was developed by applying the design characteristics of Laszlo Moholy-Nagy's paintings. With respect to the study methods, the background of Laszlo Moholy-Nagy's paintings were analyzed and their historical background was investigated by employing previous studies and literature review. The design characteristics were classified by issue and time-period. The results are as follows; First, the feasibility of creative artwork expression was found by pursuing collaboration with artwork and fashion by applying the motives of the fashion with the characteristics by the times, lines, forms, colors, and overlapping in Moholy-Nagy's paintings. Second, athleisure fashion, which can mix-match with casual wear, was found to enhance not only creativity but also add value by applying design, which added multiple handcraft elements, as well as characteristics of functionality, aesthetics, practicality, appealability, ostentation, activity, and dynamism. Third, potential harmony was found with achromatic and chromatic colors that can express more dynamic and active images. Based on the study results, further studies are anticipated using multiple paintings.

• 대한전자공학회논문지
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• 제23권5호
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• pp.666-673
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• 1986

본 논문에서는 다중고장 검출이 가능하고 부가회로가 적은 새로운 PLA 설계방식을 제안하였다. 부가회로로는 하나의 쉬프트레지스터 열과 2 비트 디코우터에 제어입력을 사용하였다. 제어입력을 사용하므로써 비트선들을 효과적으로 제어할 수 있다. 종래의 논문들에서 구체적으로 다루지 않았던 단락고장과 서로 다른 모델 간의 다중고장을 다루었으며 고장의 등가관계 및 지배고장(Dominant Faults)을 정의하여 다중고장을 해석하였다. 또한 본 설계방식을 Folded PLA에도 적용하였다.

• Journal of Microbiology and Biotechnology
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• 제23권8호
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• pp.1176-1184
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• 2013

Anti-hepatocyte growth factor (anti-HGF) monoclonal antibodies (mAbs) are potential therapeutics against various cancers. Screening for high-producer clones is a time-consuming and complex process and is a major hurdle in the development of therapeutic mAbs. Here, we describe an efficient approach that allows the selection of high-producer Chinese hamster ovary (CHO) cell lines producing the novel anti-HGF mAb SFN68, which was generated previously by immunizing HGF bound to its receptor c-Met. We selected an SFN68-producing parental cell line via transfection of the dihydrofolate reductase-deficient CHO cell line DG44, which was preadapted to serum-free suspension culture, with an SFN68-expression vector. Subsequent gene amplification via multiple passages of the parental cell line in a methotrexate-containing medium over 4 weeks, followed by clonal isolation, enabled us to isolate two cell lines, 2F7 and 2H4, with 3-fold higher specific productivity. We also screened 72 different media formulated with diverse feed and basal media to develop a suboptimized medium. In the established suboptimized medium, the highest anti-HGF mAb yields of the 2F7 and 2H4 clones were 842 and 861 mg/l, respectively, which were about 10.5-fold higher than that of the parental cell line in a non-optimized basal medium. The selected CHO cell lines secreting high titers of SFN68 would be useful for the production of sufficient amounts of antibodies for efficacy evaluation in preclinical and early clinical studies.

• Asian Pacific Journal of Cancer Prevention
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• 제15권1호
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• pp.449-454
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• 2014

Background: Extracts of Caesalpinia mimosoides Lamk has been reported to possess anticancer effects, but the active ingredients and the anti-cancer mechanisms are still unknown. Materials and Methods: The effects of a C mimosoides Lamk extract on cell proliferation and apoptosis induction in human cervical carcinoma cell lines, namely HeLa, SiHa, and C33A, as well as in normal Vero cells, were investigated. Results: Treatment with 5 active fractions (F17-F21) of C mimosoides Lamk methanol extracts inhibited cell viability in a dose- and time-dependent manner. Neutral red assays indicated that treatment with F21 significantly decreased the viability of all cervical cancer cell lines compared to F21-treated normal cells. In addition, HPLC analysis revealed that F21 contained multiple phenolic compounds, namely gallic acid, caffeine, vanillic acid, ferulic acid and resveratrol. F21 had the lowest IC50 and, therefore, a much higher cytotoxicity than F20, F17, F19, and F18 by 20-, 25-, 46- and 47- fold, respectively. Analysis of activation of the apoptosis pathway using a caspase 3/7 activity assay revealed that F21 treatment resulted in a considerable increase in caspase activation in all cancer cell lines tested. At the same concentration of F21, HeLa cells had the highest caspase activity (6.5-fold) compared to the control. Conclusion: C mimosoides Lamk may be of value as an alternative therapeutic agent, especially in combination with other compounds offering possible of synergy of action. Moreover, HPV- and non-HPV-related cervical cancer cells may differ in their responses to treatment regimens.

• BMB Reports
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• 제38권5호
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• pp.526-532
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• 2005

A lectin with in-vitro anticancer activity against established human cancer cell lines has been purified by affinity chromatography on asialofetuin-linked amino activated silica beads from the tubers of Arisaema tortuosum, popularly known as Himalayan Cobra lily, a monocot plant from the family Araceae. The bound Arisaema tortuosum lectin (ATL) was eluted with glycine-HCl buffer, pH 2.5. ATL was effectively inhibited by asialofetuin, a complex desialylated serum glycoprotein as well as by N-acetyl-D-lactosamine, a disaccharide. It gave a single band corresponding to a subunit molecular weight of 13.5 kDa in SDS-PAGE, pH 8.8 both under reducing and non reducing conditions. When subjected to gel-filtration on Biogel P-200, it was found to have a molecular weight of 54 kDa, suggesting a homotetramer structure, in which individual polypeptides are not bound to each other with disulfide bonds. ATL is a glycoprotein with 0.9% carbohydrate content, stable up to $55^{\circ}C$ and at pH 2 to 10. The lectin had no requirement for divalent metal ions i.e. $Ca^{2+}$ and $Mn^{2+}$ for its activity. However, as reported for other monocot lectins, ATL gave multiple bands in isoelectric focusing and Native PAGE, pH 8.3. The lectin was found to inhibit in vitro proliferation of human cancer cell lines HT29, SiHa and OVCAR-5.

• Journal of Crop Science and Biotechnology
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• 제10권3호
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• pp.193-200
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• 2007

Mungbean plants generally have a relatively close canopy, thus a large amount of self-shading can reduce yield due to poor light penetration. Modification of leaflet type can affect leaf canopy and could alter seed yield. Two multiple leaflet mutants were obtained from gamma-ray irradiation and used to study the mode of inheritance related to leaflet types and to evaluate their agronomic features. The cross between large-heptafoliate leaflet with small-pentafoliate leaflet mutants produce all $F_1$ plants with normal trifoliate leaflets. The $F_2$ plants segregated in leaflet size and leaflet number into a 9:3:3:1 ratio of large-trifoliate: large-heptafoliate: small-pentafoliate: small-heptafoliate plants, suggesting that independent loci control leaflet size and leaflet number. Regarding leaflet number, the $F_2$ population can be classified into normal-trifoliate, small-pentafoliate, large-heptafoliate, and small-heptafoliate at the dihybrid ratio of 9:3:3:1. The gene symbols $N_1,n_1$ and $N_2,n_2$ are proposed to represent leaflet number. Since no plant was found with large-pentafoliate leaflets, we hypothesize that the $N_2$ allele expresses pleiotropic effect on both leaflet number and leaflet size. Another possibility is that an additional locus with S and s alleles controls leaflet size and S is tightly linked with $N_2$. The effect of multifoliate leaflet on yield and yield components was evaluated in four mungbean families each with four leaflet isolines under three environments. Averaging across the families and environments, the normal-trifoliate and large-heptafoliate lines gave higher yield than small pentafoliate and heptafoliate ones. These two large leaflet lines also had higher leaf area per plant than the other multifoliate lines. Therefore, the mungbean lines with a greater leaf area, which were likely to intercept more sunlight, gave greater yield. Three AFLP markers that were found to be linked to number of leaflets per leaf, corresponded to the N1 allele of the smallpentafoliate parent.

• 한국육종학회지
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• 제42권4호
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• pp.365-373
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• 2010

TILLING (Targeting Induced Local Lesions IN Genomes) is broadly regarded as an excellent methodology for reverse genetics applications. Approximately 15,000 $M_3$ TILLING lines have been developed via the application of gamma-ray irradiation to rice seeds (cv. Donganbyeo), followed by subsequent selections. In an effort to evaluate the genetic diversity of the TILLING population, we have employed the AFLP multiple dominant marker technique. A total of 96 (0.64%) TILLING lines as well as Donganbyeo were selected randomly and their genetic diversity was assessed based on AFLP marker polymorphisms using 5 primer combinations. An average of 100.4 loci in a range of 97 to 106 was detected using these primer combinations, yielding a total of 158 (31.4%) polymorphic loci between Donganbyeo and each of the 96 lines. A broad range of similarity from 80% to 96% with an average of 89.4% between Donganbyeo and each of the 96 lines was also observed, reflecting the genetic diversity of the TILLING population. Approximately 28 polymorphic loci have been cloned and their sequences were BLAST-searched against rice whole genome sequences, resulting in 20 matches to each of the gene bodies including exon, intron, 1 kb upstream and 1 kb downstream regions. Six polymorphic loci evidenced changes in the coding regions of genes as compared to the rice pseudomolecules, 4 loci of which exhibited missense mutations and 2 loci of which exhibited silent mutations. Therefore, the results of our study show that the TILLING rice population should prove to be a useful genetic material pool for functional genomics as well as mutation breeding applications.

• 한국가정과학회지
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• 제7권3호
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• pp.35-48
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• 2004

In this study, by determining lower limb movements which cause significant changes in body surface lines, body parts with the greatest maximum expansion and contraction rate respectively were illustrated in descending order. Using unmarried female university students aged 18 - 24 as subjects, a total of 32 body surface categories (15 body surface lines and 17 body surface segment lines) were measured in one static and 9 movement poses. In particular, expansion and contraction levels and rates were measured and used in the analysis. The analysis first involved the calculation of the average measurement per body part in body surface line in static pose as well as of the average expansion and contraction levels and rates in 9 lower limb movements. Two-way MANOVA and multiple comparison analysis (Tukey) were conducted on movements and individual somatotypes regarding measurement per body part and expansion and contraction rates. Movements which cause measurements of body surface lines differed significantly in body surface line in static pose versus in movement were then identified. Among average expansion and contraction rates in such movements, maximum average expansion and contraction levels, maximum average expansion and contraction rate, and classes of expansion and contraction rate were determined per body part. The results of this study are as follows. First, 5 lower limb movements; F2, F5, F6, F7, F8, which caused significant changes in body surface lines were determined and illustrated in table 4. Second, the levels, rates, and classes of expansion and contraction rate per body part are illustrated in Tables 5 and 6. Body parts with the greatest maximum expansion rate were, in descending order: upper segment of center back leg line, upper segment of inner leg line, middle segment of center front leg line, posterior crotch length, anterior knee girth, anterior thigh girth, center back leg line, girth at crotch height, anterior midway thigh girth, hip girth, anterior crotch length, knee girth, waist girth, inner leg line, thigh girth, and crotch length. Those with the greatest maximum contraction rate were, in descending order: anterior crotch length, upper segment of center front leg line, lower segment of center back leg line, center front leg line, and posterior thigh girth. The maximum expansion rates and maximum contraction rates, which ranged from 2.05 to $35.95\%$ and from -0.20 to $-30.16\%$ respectively, were classified per body part into 4 ABCD classes. The body part with maximum expansion was the upper segment of the center back leg line at vertical body surface line, expanding by $35.95\%$ or 16.03cm in F5 flexion movement. In contrast, the body part with maximum contraction was the anterior crotch length at vertical body surface line, contracting by $-30.16\%$ or -10.54cm in F5 flexion movement. Both, however, were the body parts to expand or contract the most among all horizontal and vertical body surface lines.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.113-117
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• 2003

The digital systems include the noise in power supply, ground and packaging due to a simultaneous switching of signal, signal reflections and distortions on single and multiple transmission lines. The requirement for the controlled impedance on a PCB can be both a critical success factor and a design challenge. So, the invented tool simulates the tracks controlled impedance with the test coupon. It can saves the design time and supports the economical PCB design.

• 제어로봇시스템학회논문지
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• 제6권6호
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• pp.506-513
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• 2000

This paper proposes a path planning algorithm for dispensers to increase the productivity in printed circuit board assembly lines. We analyze the assembly sequence of the dispenser, and formulate it as an integer programming problem. The mathematical formulation can accomodate multiple heads and different types of heads through extended cost matrix. The TSP algorithms are then applied to the formulated problem to find the near-optimal solution. Simulation results are presented to verify the usefulness of the proposed scheme.