• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5409-5413
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• 2012

At present, multiple myeloma (MM) remains an incurable disease and cologenic cells may be responsible for disease relapse. It has been proposed that CD20+/CD138- NCI-H929 cells could be hallmarks of MM clonogenic cells. Here, the immunology phenotype of NCI-H929 cells is described. Only a small population of CD20+/CD138- cells (<1%) was found in the NCI-H929 cell line, but CD20+/CD138- cells were not detected. We found that CD20+/CD138+ cells were able to exhibit cologenic capacity by colony formation assay and continuous passage culture. Proteins were analyzed by 1D-SDS-PAGE and TMT based quantitative differential liquid chromatography tandem mass spectrometry (LC-MS/MS). 1,082 non-redundant proteins were identified, 658 of which were differentially expressed with at least a 1.5-fold difference. 205 proteins in CD20+ cells were expressed at higher levels and 453 proteins were at lower levels compared with CD20- cells. Most proteins had catalytic and binding activity and mainly participated in metabolic processes, cell communication and molecular transport. These results proved that there are different biological features and protein expression profile between CD20+ and CD20- cells in the NCI-H929 cell line.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.166-174
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• 2021

Multiple myeloma is a malignant cancer of plasma cells. Despite recent progress with immunomodulatory drugs and proteasome inhibitors, it remains an incurable disease that requires other strategies to overcome its recurrence and non-response. Based on the high expression levels of programmed death-ligand 1 (PD-L1) in human multiple myeloma isolated from bone marrow and the murine myeloma cell lines, NS-1 and MOPC-315, we propose PD-L1 molecule as a target of anti-multiple myeloma therapy. We developed a novel anti-PD-L1 antibody containing a murine immunoglobulin G subclass 2a (IgG2a) fragment crystallizable (Fc) domain that can induce antibody-dependent cellular cytotoxicity. The newly developed anti-PD-L1 antibody showed significant antitumor effects against multiple myeloma in mice subcutaneously, intraperitoneally, or intravenously inoculated with NS-1 and MOPC-315 cells. The anti-PD-L1 effects on multiple myeloma may be related to a decrease in the immunosuppressive myeloid-derived suppressor cells (MDSCs), but there were no changes in the splenic MDSCs after combined treatment with lenalidomide and the anti-PD-L1 antibody. Interestingly, the newly developed anti-PD-L1 antibody can induce antibody-dependent cellular cytotoxicity in the myeloma cells, which differs from the existing anti-PD-L1 antibodies. Collectively, we have developed a new anti-PD-L1 antibody that binds to mouse and human PD-L1 and demonstrated the antitumor effects of the antibody in several syngeneic murine myeloma models. Thus, PD-L1 is a promising target to treat multiple myeloma, and the novel anti-PD-L1 antibody may be an effective anti-myeloma drug via antibody-dependent cellular cytotoxicity effects.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• 제15권4호
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• pp.119-125
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• 2015

In this paper, we introduce a Hadamard matrix interstream transmission based blind channel estimation for multi-cells multiple-input and multiple-output (MIMO) networks. The proposed scheme is based on a network with mobile stations (MS) which are deployed with multi cells. We assume that the MS have the signals from both cells. The signal from near cell are considered as desired signal and the signals from the other cells are interference signal. Since the channel is blind, so that we transmit Hadamard matrix pattern pilot stream to estimate the channel; that gives easier and fast channel estimation for large scale MIMO channel. The computation of Hadamard based system takes only complex additions, and thus the complexity of which is much lower than the scheme with Fourier transform since complex multiplications are not needed. The numerical analysis will give perfection of proposed channel estimation.

• Korean Journal of Veterinary Pathology
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• 제7권1호
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• pp.63-65
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• 2003

A 10-year-old Korean native female Jin-do dog revealed two growing subcutaneous masses measuring 7.0 ${\times}$ 7.0 ${\times}$ 2.5 cm and 5.0 ${\times}$ 4.0 ${\times}$ 2.0 cm in the left shoulder and lower part of chest, respectively. Grossly tumor masses were well-circumscribed and exhibited ulcerated surface and purulent exudate on the cut surface. Histologically tumor masses were characterized by cystic structures some of which were fused together. Although the cells of cyst wall differentiated to those similar to infundibulum, isthmus, inferior segment of hair follicles, respectively, the most cyst wall consisted of cells similar to infundibulum The luminal content of cysts depended on the cells of cysts; infundibulum-like cyst contained lamellar keratin, isthmus-like cyst was amorphous keratin, and inferior segment-like cyst had shadow cells. From these results, these tumor masses were diagnosed as multiple trichoepithelioma. To our knowledge, this is the first report of canine multiple trichoepthelioma in a dog in Korea.

• BMB Reports
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• 제48권4호
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• pp.193-199
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• 2015

Degenerative retinal diseases affect millions of people worldwide, which can lead to the loss of vision. However, therapeutic approaches that can reverse this process are limited. Recent efforts have allowed the possibility of the stem cell-based regeneration of retinal cells and repair of injured retinal tissues. Although the direct differentiation of pluripotent stem cells into terminally differentiated photoreceptor cells comprises one approach, a series of studies revealed the intrinsic regenerative potential of the retina using endogenous retinal stem cells. Muller glial cells, ciliary pigment epithelial cells, and retinal pigment epithelial cells are candidates for such retinal stem cells that can differentiate into multiple types of retinal cells and be integrated into injured or developing retina. In this review, we explore our current understanding of the cellular identity of these candidate retinal stem cells and their therapeutic potential for cell therapy against degenerative retinal diseases. [BMB Reports 2015; 48(4): 193-199]

• The Journal of the Korean life insurance medical association
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• 제29권2호
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• pp.33-35
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• 2010

Multiple myeloma is characterized by the neoplastic proliferation of a single clone of plasma cells producing a monoclonal immunoglobulin and it is frequently associated with primary amyloidosis. I experienced a medical claims review case of plasma cell dyscrasia with primary amyloidosis. This medical consulting work to insurance claims will be helpful for another similar claims administration.

• Structural Engineering and Mechanics
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• 제5권1호
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• pp.21-32
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• 1997

A new torsional analysis method for multiple cell box based on the Softened Truss Model Theory was developed. This softened truss model unifies shear and torsion to address the problem associated with a torque applied on a box. The model should be very useful for the analysis of a reinforced concrete box under torque, especially for the bridge superstructure with multiple cell box sections.

• Biomolecules & Therapeutics
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• 제26권4호
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• pp.380-388
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• 2018

Neural stem cells (NSCs) have the ability to self-renew and differentiate into multiple nervous system cell types. During embryonic development, the concentrations of soluble biological molecules have a critical role in controlling cell proliferation, migration, differentiation and apoptosis. In an effort to find optimal culture conditions for the generation of desired cell types in vitro, we used a microfluidic chip-generated growth factor gradient system. In the current study, NSCs in the microfluidic device remained healthy during the entire period of cell culture, and proliferated and differentiated in response to the concentration gradient of growth factors (epithermal growth factor and basic fibroblast growth factor). We also showed that overexpression of ASCL1 in NSCs increased neuronal differentiation depending on the concentration gradient of growth factors generated in the microfluidic gradient chip. The microfluidic system allowed us to study concentration-dependent effects of growth factors within a single device, while a traditional system requires multiple independent cultures using fixed growth factor concentrations. Our study suggests that the microfluidic gradient-generating chip is a powerful tool for determining the optimal culture conditions.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6757-6760
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• 2013

Gastric cancer is one of the most frequently occurring malignancies in the world. Development of multiple drug resistance (MDR) to chemotherapy is known as the major cause of treatment failure for gastric cancer. Multiple drug resistance 1/P-glycoprotein (MDR1/p-gp) contributes to drug resistance via ATP-dependent drug efflux pumps and is overexpressed in many solid tumors including gastric cancer. To investigate the role of MDR1 knockdown on drug resistance reversal, we knocked down MDR1 expression using shRNA in drug resistant gastric cancer cells and examined the consequences with regard to adriamycin (ADR) accumulation and drug-sensitivity. Two shRNAs efficiently inhibited mRNA and protein expression of MDR1 in SGC7901-MDR1 cells. MDR1 knockdown obviously decreased the ADR accumulation in cells and increased the sensitivity to ADR treatment. Together, our results revealed a crucial role of MDR1 in drug resistance and confirmed that MDR1 knockdown could reverse this phenotype in gastric cancer cells.

• Journal of Korea Multimedia Society
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• 제7권5호
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• pp.737-743
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• 2004

In this paper, we propose the call control scheme that can improve the capacity of the wireless system for the non-uniform traffic load distribution and the multiple types of services in multiple cells CDMA system. The number of mobile stations that can be served simultaneously in a base station is limited by the amount of total interference received in CDMA system. Further, the average number of mobile stations in each cell may not be uniformly distributed. Considering this factors, the call admission control scheme using the effective bandwidth concept is adapted in this paper. Thus, the bandwidth for a new call can be varied dynamically for reducing the blocking rate of new calls and the dropping rate of handoff calls. The suggested call control scheme is experimented through a simulation by dynamically assigning the bandwidth to new and handoff calls. The simulation results show that the proposed call control scheme can accommodate more mobile stations than the other methods in multiple cells environment.