• Journal of Microbiology and Biotechnology
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• 제21권12호
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• pp.1306-1311
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• 2011

Recombinant Rhodopseudomonas palustris, harboring the carotenoid-metabolizing gene crtI (CrtIBS), and whose color changes from greenish yellow to red in response to inorganic As(III), was cultured in transparent microplate wells illuminated with a light emitting diode (LED) array. The cells were seen to grow better under near-infrared light, when compared with cells illuminated with blue or green LEDs. The absorbance ratio of 525 to 425 nm after cultivation for 24 h, which reflects red carotenoid accumulation, increased with an increase in As(III) concentrations. The detection limit of cultures illuminated with near-infrared LED was 5 ${\mu}g$/l, which was equivalent to that of cultures in test tubes illuminated with an incandescent lamp. A near-infrared LED array, in combination with a microplate, enabled the simultaneous handling of multiple cultures, including CrtIBS and a control strain, for normalization by the illumination of those with equal photon flux densities. Thus, the introduction of a near-infrared LED array to the assay is advantageous for the monitoring of arsenic in natural water samples that may contain a number of unknown factors and, therefore, need normalization of the reporter event.

• 한국식품영양학회지
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• 제30권5호
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• pp.867-879
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• 2017

This study investigated the factors affecting problematic drinking amongst the male university students of the Changwon area (n=367). The participants were divided into 3 groups, and undertook the self-report questionnaire survey. Based on the AUDIT (Alcohol Use Disorders Identification Test) guidelines, problematic drinking were rated as 'no problem' (28.8%), 'at-risk drinking' (41.7%), and 'alcohol abuse' (29.5%). Our study revealed that students living alone, indulged in more club activities, and drank more than 3 cans of carbonated drinks a day; this was significantly high on the AUDIT score (p<0.05). Participation in education was significantly higher (p<0.05), but intention to reduce the monthly alcohol consumption was significantly lower (p<0.001) in the alcohol abuse group. Recognition level of drinking cultures, drinking habits, AUDIT, and nicotine dependence were significantly higher in the alcohol abuse group (p<0.001). Recognition level of drinking cultures and nicotine dependence by the FTND (Fagerstrom Test for Nicotine Dependence) positively correlated with problematic drinking, whereas dietary guidelines and self-esteem showed a negative correlation with problematic drinking (p<0.05). Based on the results of multiple linear regression analysis, the factors affecting problematic drinking were recognition level of drinking cultures (${\beta}=0.47$, p<0.001) and nicotine dependence (${\beta}=0.23$, p<0.001). We conclude that implementation of health education for university students, would aid in rectifying the incorrect perception of drinking. Furthermore, both drinking and smoking should be considered simultaneously.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권8호
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• pp.3258-3275
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• 2015

The rise in recent technology has changed the ways, in which people communicate with one another. Social networking services (SNSs) have become one of the most representative means. General SNSs allow users to create their own unique profiles, search for fellow members, share information, etc., while other SNSs have functions that cater to different needs of users. As a result, users of SNSs have begun to pick and choose different SNSs and concurrently use multiple SNSs in order to fulfill all their needs. This exploratory study examined which SNSs are used together and the characteristics that predict the use of multiple SNSs. In addition, it observed the differences between consumers' usage of multiple SNSs in different cultures. An online survey was administered to SNS users in the United States and Korea. The results of the study showed that the use of multiple SNSs is not yet prevalent in Korea, the country that represented a collectivistic culture. In addition, in the U.S., the highest number of users reported that they were active on at least three SNSs.

• 대한수의학회지
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• 제20권2호
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• pp.85-92
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• 1980

The antimicrobial drug susceptibility of 439 isolates of animal pathogens recovered from various clinical cases during 1978-79 has been investigated by the use of disk diffusion technique. The majority of 308 strains of Eschericihia coli were highly resistant to bacitracin, erythromycin, penicillin, streptomycin and tetracyclinon while only 0.3 per cent of them were resistant to gentamicin and 3.2 per cent to colistin. The percentages of strains resistant to ampicillin, carbenicillin, cephalothin, chloramphenicol and neomycin were 30.5%, 24.7%, 11:4%, 28.2% and 26.2% and repectively. However, none of E. coli cultures of ovine origin were resistant to ampicillin, carbenicillin, chloramphenicol, colistin, gentamicin, kanamycin, and neomycin. A total of 39 patterns of multipe drug1 resistance of 308 strains E. coli against 9 drugs in general use such as ampicillin, cephalothin, chloramphenicol, colistin, gentamicin, kanamycin, neomycin, streptomycin and tetracycline were observed and the most common multiple resistance patterns were SM, TC pattern (20.5%) and AM, CP, KM, NM, SM, TC pattern (9.7%). None of the 43 cultures of salmonella organism from pigs and chickens were resistant to ampicillin, carbenicillin, cephalothin, colistin, gentamicin and kanamycin; and the majority of the cultures were susceptible to chloramphenicol (90.0%), neomycin (97.7%) and tetracycline (93.0%). All the cultures were found to be resistant to bacitracin and penicillin and the rate of resistant strains to erythromycin and s treptomycin being 79.1% and 41.9% respectively. It was found that the majority of 63 cultures of staphylococcal isolates were resistant to lincomycin, penicillin, streptomycin and tetracycline. The percentages of 63 staphylococcal isolates susceptible to gentamicin, nitrofurantoin, cephalothin, ampicillin, methicillin, bacitracin and chloramphenicol were 98.4%, 98.4%, 95.2%, 93.7%, 93.7%, 92.1% and 92.1% respectively. The 25 cultures of streptococcal isolates were resistant in order of prevalence to streptomycin(88.0%), kanamycin(68.0%), gentamicin (44.0%), tetracycline (44.0%) and methicillin (40.0%) wihle the majority of them were sensitive to ampicillin, bacitracin, chloramphenicol and penicillin.

• 한국동물위생학회지
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• 제44권4호
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• pp.305-308
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• 2021

A female Chinese hamster with unknown age was referred for acute onset of anorexia, depression, and large cyst from the head to body. After referring, the patient died shortly. During necropsy, severe hemorrhage in the cyst, multiple mass on liver, and transformed right kidney were found. The infection was confirmed by cytology, cultures and PCR of 16S ribosomal RNA gene. This report describes a first case of naturally occurred systemic Klebsiella oxytoca infection in a household Chinese hamster.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.577-581
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• 2003

Streptomyces lividans is one of the most commonly-used streptomyetes strain as a molecular cloning and expression host. Unlike its close relative S. coelicolor, however, S. lividans rarely produces secondary metabolite such as actinorhodin in a typical glucose-containing culture condition due to insufficient expression of some antibiotic regulatory genes including afsR2. Although multiple copies of afsR2 or a glycerol-specific culture condition stimulated actinorhodin production in S. lividans, both failed to stimulate actinorhodin production in S. lividans cultured in a typical glucose-containing medium. To generate a culture-condition-independent actinorhodin-overproducing S. lividans strain the afsR2 gene was integrated into the S. lividans TK21 chromosome via homologous recombination, followed by the genetic confirmation. This S. lividans strain produced a significant amount of actinorhodin in both glucose-containing liquid and plate cultures, with higher actinorhodin productivity compared to the S. lividans containing multiple copies of afsR2. These results suggest that a chromosomal integration of a single copy of an antibiotic regulatory gene is a promising method for the development of a stable antibiotic-overproducing streptomycetes strain.

• 디자인학연구
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• 제19권3호
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• pp.71-92
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• 2006

People have their own cultural backgrounds and experiences in terms of visual perceptional thinking so that they could misunderstand the cultural symbols, trademarks, Brand Identities, and trade characters, especially, animal trademarks. Sometimes Easterners and Westerners seem to not comprehend the different meanings of the cultural symbols. The signs of twelve Chinese zodiac animals are the typical symbol of the Asian mythology. What I wanted to focus on emphasizing the different views and perspective of an animal trademark is according to the Chinese Zodiac between Eastern and Western cultures. Generally, multiple symbolisms are difficult to comprehend, because they are created by individual ideas and incorporate several mythologies and histories. How do Westerners interpret the implied meaning of Eastern animals in general? How are they going to understand the Eastern animal logos or marks accurately? How can we solve the problem to make people understand their different meanings? There were some confusing pictorial images in the area of the design field when a designer creates an animal mark for globalization and localization. Creating of hybrid symbol is the best way to break the communication barriers with people all around the world.

• 수산해양교육연구
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• 제22권1호
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• pp.11-24
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• 2010

The purpose of this study was to analyze the needs of the preservice secondary school teacher on understanding of international society and curriculum of international understanding and to derive the implications on developing curriculum of international understanding. This study used a questionnaire survey. The results are summarized as follows. First, it seems to be necessary to develop curriculum for satisfying the higesconcerns on worldsculture of preservice secondary school teacher. Second, the curriculum of educating international understanding for preservice secondary school teacher should contain such contents as multiple cultures, multiple perspectives, global relations and instructional materials. Third, it is necessary that instructions on educating international understanding should employ experience of global culture, discussion, lecture and so on.

• 식물조직배양학회지
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• 제28권1호
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• pp.25-32
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• 2001

우리나라 주요 과수류중 하나인 포도의 경정배양을 통하여 증식재료로 이용할 수 있는 다아체의 형성 및 이들의 신장에 미치는 생장조절물질의 영향에 관해 검토하였다. 경정배양 시 재료의 살균은 5월 초순에 생육이 왕성한 신초를 채취하여 NaOCl 1%용액에 10분간 살균한 것에 비해 Wilson액에 5분간 침지 살균했을 때 생존율이 높았다. 다아체의 증식은 3/4 MS 기본배지에 NAA 0.1 mg/L와 BA 3.0mg/L에서 양호하였다. 다아체로부터 신초의 신장은 3/4 MS배지에 AdSO$_4$80 mg/L, NAA 0.1 mg/L와 BA를 1.0~2.0 mg/L 혼합한 배지에서 양호하였으며 품종간 생장조절물질의 적정농도 차이는 거의 없었다.

• 한국콘텐츠학회논문지
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• 제9권2호
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• pp.279-288
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• 2009

본 연구의 목적은 병원의 정규직원과 아웃소싱 직원간의 일반적 특성을 이해하고, 아웃소싱에 대한 태도에 있어서 차이를 분석하며, 근무환경, 가치관, 조직문화요인 등의 요소들을 비교분석하고, 이러한 요인들을 최적화하여 직무만족과 직무몰입, 이직의도에 각기 어떠한 영향을 미치는가를 분석하고자 하였다 설문조사 결과, 정규직원들이 직업에 대한 자부심, 직무수행능력에 대한 인정수준, 상사에 대한 업무지시 방식에 대한 만족도, 하루업무량에 대한 만족도 등에 대한 내용들에 있어서 아웃소싱 직원에 비해 긍정적으로 인식하고 있었고, 통계적으로도 유의한 결과를 보였다. 또한, 다중회귀분석을 통해 직무만족과 직무몰입에 영향을 미치는 조직문화 요인으로, 정규 직원에게서는 이념수행과 가치관이, 아웃소싱 직원에게서는 가치관과 이념인식, 교육이 영향을 미치는 요인으로 나타났다. 그러므로 직무만족과 직무몰입을 극대화하기 위해서는 아웃소싱 직원들의 긍정적인 가치관 확립을 위한 프로그램의 개발과 함께 직원 채용시 긍정적 가치관을 소유하고 있는 직원을 선발하는 것이 매우 중요하다고 할 수 있다.