• Korean Journal of Ecology and Environment
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• v.38 no.1 s.110
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• pp.18-29
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• 2005

This study was conducted to understand seasonal dynamics of phyto- and zooplankton communities in a shallow eutrophic reservoir (Shingu reservoir) from November 2002 to February 2004. Cyanophyceae dominated throughout the year, except for spring (March ${\sim}$ May) when Bacillariophyceae (Melosira varians) and Chlorophyceae (Dictyosphaerium puchellum) were dominant. The change of dominant species in Cyanophytes occurred in June and December 2003, and the increase of phytoplankton cell density in July and November was observed when the P loading through two inflows was high. In May, Oscillatoria spp. and Aphanizomenon sp. were dominant, but replaced by Microcystis spp. in the end of May. Dominant Microcystis spp. sustained until December and shifted to Oscillatoria spp. and Aphanizomenon sp. TN/TP ratio ranged from 13 to 46 (Avg. $27{\pm}6$) from June to December when cyanobacteria (Microcystis spp.) dominated. Rotifers such as Keratella cochlearis, Keratella valga, Polyarthra spp., Conochilus unicornis, Pompholyx complanata dominated in average 67.8% of the zooplankton community. Abundance of zooplankton was the highest in June 2003, when Pompholyx complanata (12,388 ind $L^{-1}$) was dominant. In May, the significant increase of Conochilus unicornis biomass ($1,048{\pm}28\;{\mu}g\;C\;L^{-1}$) was observed with distinct improvement of transparency ($Z_{eu}/\;Z_m=\;1.1$). These results suggest that the seasonal variation of phytoplankton communities in this reservoir are to be understood as results of multi-interactive factors such as temperature, light condition and nutrients, and small-sized rotifers as important predator.

• Economic and Environmental Geology
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• v.40 no.5
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• pp.563-574
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• 2007

Laser induced breakdown spectroscopy (LIBS) is a recently developed analytical technique that is based upon the measurement of emission lines generated by atomic species close to the surface of the sample, thus allowing their chemical detection, identification and quantification. With powerful advantages of LIBS compared to the conventional analytical methodology, this technique can be applied in the detection of heavy metals in the field. LIBS allows the rapid analysis by avoiding laborious chemical steps. LES have already been applied for the determination of element concentration in a wide range of materials in the solid, liquid and gaseous phase with simplicity of the instrument and diversity of the analytical application. These feasibility of rapid multi elemental analysis are appealing proprieties for the in-situ analytical technique in geochemical investigation, exploration and environmental analysis. There remain still some limitations to be solved for LIBS to be applied in soil environment as an in-situ analytical technology. We would like to provide the basic principle related to the plasma formation and laser-induced breakdown of sample materials. In addition, the matrix effect, laser properties and the various factors affecting on the analytical signal of LIBS was dealt with to enhance understanding of LIBS through literature review. Ultimately, it was investigated the feasibility of LIBS application in soil environment monitoring by considering the basic idea to enhance the data quality of LIBS including the calibration method for the various effects on the analytical signal of LIBS.

• Journal of Environmental Impact Assessment
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• v.20 no.2
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• pp.187-198
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• 2011

The objective of this study is to analyze the flora and forest vegetation of trails with high visitor density at Molundae. Nine quadrats of $20{\times}20m$ were selected for the survey. The survey was conducted from April to October 2010. The obtained results are summarized as follows. Plot1, plot2, plot3, plot4, plot6, and plot7 were located at slopes of $5{\sim}20^{\circ}$, 17~40m above sea level, and were formed with the colony of Japanese black pine(Pinus thunbergii Parl) and Japanese black pine(Pinus thunbergii Parl)-white oak(Quercus aliena Blume). Tree layer had the height of 8~20m, and the coverage of 50~70%, while subtree layer had the height of 3-8m, and the coverage 30~80%. On the other hand, shrub layer had the height of 2~4m, and the coverage of 10~30%, and herb had the height of 0.2~1m and coverage 5~20%. Plot5, plot8, and plot9 were located at the summit areas of 57~78m above sea level, and $0^{\circ}$ slope. Japanese black pine(Pinus thunbergii Parl) formed a community there. Tree layer was 8~20m high, and covered 60~70%, of the area, and subtree layer was 6~8m high, and coverage 30~40%. Shrub layer had the height of 2~6m, and the coverage of 30%, while herb layer had the height 0.2~2m, and the coverage 20-80%. Succession does not occur in the surveyed areas which have high visitor density. Artificially planted sawtooth oak(Quercus acutissima) trees were found to disturb succession and formation of multi-layer vegetation, resulting in the ecologically unstable forest. Therefore, the researcher suggested the strategy of managing the vegetation in the conclusion. This study has the limit in that the plots selected for the survey reflected only part of various trails in the Molundae area. It is necessary to suggest the vegetation management plans by selecting more diverse trail areas in consideration of the visitor density and behaviors, and analyzing the changes in vegetation quantitatively in order to manage the vegetation in urban areas damaged by visitors more effectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.2
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• pp.93-102
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• 2017

In this study, various plant extracts were screened for the effective and safe skin care ingredient according to the antioxidant activity assay guidelines. We selected an optimized combination herbal extract, Charmzone extract (CZE), which composed of Nelumbo nucifera leaves, Saururus chinensis and Orostachys japonica. CZE exerted free radical scavenging activity. It reduced reactive oxygen species formation and increased total antioxidant capacities in human keratinocyte (HaCaT) and normal human dermal fibroblasts (NHDF). CZE also leaded procollagen type 1 secretion in NHDF and decreased cellular melanin contents in B16F10. The production of nitric oxide was decreased by CZE in lipopolysaccharide-stimulated RAW264.7 in a dose dependent manner. Therefore, it is concluded that CZE can be applied for naturally derived anti-aging functional skin care ingredient for anti-oxidation, wrinkle enhancement, whitening, anti-inflammation and wound healing of skin.

• The Journal of Fisheries Business Administration
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• v.35 no.2
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• pp.91-115
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• 2004

The main purpose of this study is to analyze the cooperative squid fisheries problems surrounding the $E128^{\circ}$, established by the 1965 Korea - Japan Fisheries Agreement which banned Korean large trawlers' fishing east to the $E128^{\circ}$ In fact, the moratorium was put on by Japans request. However, such issue did not occur until the filefish stock in the southern Korean sea, which was a major target fish species of the large trawl fisheries, The filefish stock collapsed completely around 1991 and at the same time most of bottom fish stocks in the East China Sea began to show a symptom of over - exploitation. Thus, the off - shore large trawlers learned to have a little opportunity of finding out alternative fish stocks as well as fishing grounds. Fortunately, at that time squid resource stock and consumption were on the increasing trend. The large trawl fisheries were able to economically exploit squid stock east to the $E128^{\circ}$ through cooperative fishing with squid angling light boats in the East and East - South Sea, even though such cooperative fishing activities violate the existing fishery laws apparently. Some important reasons that the large trawlers have continued the cooperative fishing seem to be because (ⅰ) squid resource stock has been on the increasing state over time, (ⅱ) the trawl fisheries have made a significant contribution to meeting domestic and export demands and stabilizing squid prices, and (ⅲ) they have kept domestic squid market from foreign competition. However, the new Korea - Japan fisheries agreement in 1998 provided a momentum of questioning the effectiveness of the $E128^{\circ}$ by the squid - related fisheries other than the squid angling. Serious conflicts between squid - related fisheries began to emerge and to be much intensified. Squid angling industries in the East opposed to large trawlers's efforts to formalize such illegal cooperative squid fishing activities. Their main argument was that such formalizing would definitely make the East coast squid prices lower and in turn their business performance would be worse off. The results of quantitative analysis suggest that the trawlers' massive landing may have a significant influence on lowering the east coast squid prices. Now, an important issue that the squid - related fisheries and the government are facing is to solve such complex squid fishing problems through a multi - participatory negotiation process, including price stabilization, total allowable catch level and its operation schemes, $E128^{\circ}$ rearrangement, and so on.

• Korean Journal of Environment and Ecology
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• v.18 no.2
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• pp.236-248
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• 2004

This study was carried out to propose improvement planting method by the research and analysis of planting concept, planting density, planting style and pattern in apartment complex, Seoul. Survey sites were selected by reflecting the change of green area ratio : Hawgok Jugong apartment complex, Gangseo-gu(1974), Samik-green apartment complex, Gangdong-gu(1980), Dongsindaea apartment complex, Gangseo-gu(1992). Green area in apartment complex was classified with front green area, side green area, and back-side green area. Planting concept that composed of landscape planting concept but anyother concept was not, was similar to all sites not differ from creation time. And planted species was not differ from planting style. Planting density was of both conopyㆍunder story layer was 0.0∼0.2 tree/$m^2$, and that of shrub layer was 0.0∼0.5 tree/$m^2$ Shrub layer planting density was insufficient and the density was not changed according to the creation time. Canopyㆍunderstory and shrub was planted to another green space, not concern with multi-layer structure. Planting pattern was utilized to single planting, linear planting, and random triangle planting, but it was not to the change that in each green space planting concept. Green area in apartment complex should be variety according to planting density, planting structure and planting pattern. And we should get the function of covering and beauty in case of front green space, that of ecological environment and increasing green volume in case of back-side green area, that of increasing green volume in case of side green area, apartment complex.

• Korean Journal of Plant Taxonomy
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• v.40 no.1
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• pp.50-58
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• 2010

The nutlet morphology and pericarp structure of 14 taxa (4 taxa of the genus Glechoma and related taxa) were studied by light and scanning electron microscopy, and a detailed description of nutlet morphological features for all examined taxa is provided. The shape of the nutlet is ovate to elliptic. Its size is 1.36-3.83 mm in length and 0.80-2.65 mm in width. The largest one was found in Marmoritis rotundifolia, while the smallest one was in Agastache nepetoides. Three different surface types were recognized in the studied taxa based on differences in cell outline and cell boundary relief (rectangular, pitted, and ridged negative reticulate-cell). Three types of trichome (uni-cellular non-glandular trichome, multi-cellular non-glandular trichome, and peltate glandular trichome) were distributed in the nutlets. The pericarp is formed in three layers (i.e., exocarp, mesocarp, and endocarp including sclenchyma tissue), and the pericarp in the studied species is $39.0-237.5{\mu}m$ thick. The morphological and anatomical features (the nutlet shape, surface type, and trichome distribution) in the genus Glechoma and related taxa are described and discussed.

• KSBB Journal
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• v.21 no.2
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• pp.133-139
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• 2006

In 'solid-phase' PEGylation, the conjugation reaction occurs as the proteins are attached to a solid matrix, and thus it can have distinct advantages over the conventional, solution-phase process. We report a case study: rhIFN-${\alpha}$-2a was first adsorbed to cation exchange resin and then N-terminally PEGylated by aldehyde mPEG of 5, 10, and 20 kD through reductive alkylation. After the PEGylation, salt gradient elution efficiently recovered the mono-PEGylate in a purified form from the unwanted species such as unmodified IFN, unreacted PEG, and others. The mono-PEGylation and its purification were integrated in a single chromatographic step. Depending on the molecular weight of the mPEG aldehyde used, the mono-PEGylation yield ranged 50-64%. We could overcome the major problems of random, or uncontrollable, multi-PEGylation and the post-PEGylation purification difficulties associated with the solution-phase process. N-terminal sequencing and MALDI-TOF MS confirmed that a PEG molecule was conjugated only to the N-terminus. Compared with the unmodified IFN, the mono-PEGylate showed the reduced anti-viral activity as measured by the cell proliferation assay. The bioactivity was reduced more as the higher molecular weight PEG was conjugated. Immunoreactivity, evaluated indirectly by antibody binding activity using a surface plasmon resonance biosensor, also decreased. Nevertheless, trypsin resistance as well as thermal stability was considerably improved.

• Korean Journal of Food Science and Technology
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• v.48 no.5
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• pp.454-460
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• 2016

In this study, we investigated the possibility of Droplet digital PCR (ddPCR) for detection of foodborne pathogens. ddPCR combines partitioning of PCR reactions into several thousands or millions of individual droplets in a water-oil emulsion, and counting of positive PCR reaction using flow cytometry. Four species of foodborne pathogens, Bacillus cereus, Staphylococcus aureus, Salmonella Typhimurium and Escherichia coli O157:H7, were used to quantify the target sequence with each of the designed primers and double stranded DNA-binding Evagreen dye. All tested foodborne pathogens showed a detection limit ranging from $100fg/{\mu}L$ to $10ng/{\mu}L$. It was concluded that ddPCR could be used to detect very low concentrations of foodborne pathogens from complex food matrices. For multi-detection of target pathogens, we also tested the samples using multiplex ddPCR and obtained successful results.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.11 no.6
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• pp.123-132
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• 2012

Gas sensors has been used very differently that depending on following purposes; Automotive (exhaust gas, fuel mixture gas, oxygen, particulates), agriculture / food industry (fresh, stored, CO2, humidity, NH3, nitrogen oxide gas, organic gas, toxic gas emitted from pesticides and insecticides), industrial / medical (chemical gas, hydrogen, oxygen and toxic gases), military (chemical weapon), environmental measurements (CO and other air pollution consisting of sulfur and nitrogen gas), residential (LNG, LPG, butane, indoor air, humidity). The types of industrial toxic substances are known about 700 species and many of these exist in gaseous form under normal conditions. he multi-gas detection sensors will be developed for casualties that detect the most important and find easy three kinds of gases in marine plant; carbon dioxide(CO2), carbon(CO), ammonia(NH3). Package block consists of gas sensing device minor ingredient, rf front end, zigbee chip. Develope interworking technology between the sensor and zigbee chip inside a package. Conduct a performance test through test jig about prototype zigbee sensor module with rf output power and unwanted emission test. This research task available early address when poisonous gas leaked from large industrial site and contribution for workers' safety at the enclosed space.