• Proceedings of the IEEK Conference
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• 1998.10a
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• pp.1289-1292
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• 1998

HCI(human computer interface) technologies have been often implemented using mouse, keyboard and joystick. Because mouse and keyboard are used only in limited situation, More natural HCI methods such as speech based method and gesture based method recently attract wide attention. In this paper, we present multi-modal input system to control Windows system for practical use of multi-media computer. Our multi-modal input system consists of three parts. First one is virtual-hand mouse part. This part is to replace mouse control with a set of gestures. Second one is Windows control system using speech recognition. Third one is Windows control system using gesture recognition. We introduce neural network and HMM methods to recognize speeches and gestures. The results of three parts interface directly to CPU and through Windows.

• Journal of Institute of Control, Robotics and Systems
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• v.17 no.11
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• pp.1168-1172
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• 2011

Beam projector is popularly used for presentation. In order to pay attention to local area of the beam projector display, a laser pointer is used together with a pointing device(Mouse). Simple wireless presenter has limited functions of a pointing device such as "go to next slide" or "back to previous slide" in a specific application(Microsoft PowerPoint) through wireless channel; thus, there is inconvenience to do other tasks e.g., program execution, maximize/minimize window etc. provided by clicking mouse buttons. The main objective of this paper is to implement a multi-functional laser-pointer mouse that has the same functions of a computer mouse. In order to get position of laser spot in the projector display, an image processing to extract the laser spot in the camera image is required. In addition, we propose a transformation of the spot position into computer display coordinates to execute mouse functions on computer display.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.2
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• pp.167-173
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• 2015

Extraction of Finger-end points is one of the most process for user multi-commands in the Hand-Gesture interface technology. However, most of previous works use the geometric and morphological method for extracting a finger-end points. Therefore, this paper proposes the method of user finger-end points extraction that is motivated a ultrared diffused illumination, which is used for the user commands in the multi-touch display device. Proposed air-mouse is worked by the quantity state and moving direction of extracted finger-end points. Also, our system includes a basic mouse event, as well as the continuous command function for expending a user multi-gesture. In order to evaluate the performance of the our proposed method, after applying to the web browser application as a command device. As a result, the proposed method showed the average 90% success-rate for the various user-commands.

• The Korea Journal of Herbology
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• v.23 no.2
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• pp.151-157
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• 2008

Objectives : The purpose of this study is to investigate the biological Effect of multi-herbal drug 'KOCO-P1' on mouse macrophage Raw 264.7 cells. Methods : Multi-herbal drug 'KOCO-P1' was composed of Ginseng Radix, Astragali Radix, Polygonati Rhizoma, Liriopis Tuber, and Scrophulariae Radix. Cytotoxicity and cytoprotective activity of K0C0-P1 was verificated by MTT assay. And antioxidative effect of K0C0-P1 against EtOH, Nicotine was inspected by Hydroperoxide assay. Results : K0C0-P1 showed no cytotoxicity on RAW 264.7 cells for 24, 48, 72 hours. KOCO-P1 at 200, 100, and 50 ug/mL reduced the production of H202 in Raw 264.7 cells by EtOH. KOCO-P1 at 50 ug/mL reduced the production of H202 in Raw 264.7 cells by Nicotine. Conclusions : KOCO-P1 could be supposed to have antioxidative effect on macrophage with no cytotoxicity.

• Laboraroty Animal Research
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• v.34 no.4
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• pp.279-287
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• 2018

Placenta specific 8 (PLAC8, also known as ONZIN) is a multi-functional protein that is highly expressed in the intestine, lung, spleen, and innate immune cells, and is involved in various diseases, including cancers, obesity, and innate immune deficiency. Here, we generated a Plac8 knockout mouse using the CRISPR/Cas9 system. The Cas9 mRNA and two single guide RNAs targeting a region near the translation start codon at Plac8 exon 2 were microinjected into mouse zygotes. This successfully eliminated the conventional translation start site, as confirmed by Sanger sequencing and PCR genotyping analysis. Unlike the previous Plac8 deficient models displaying increased adipose tissue and body weights, our male Plac8 knockout mice showed rather lower body weight than sex-matched littermate controls, though the only difference between these two mouse models is genetic context. Differently from the previously constructed embryonic stem cell-derived Plac8 knockout mouse that contains a neomycin resistance cassette, this knockout mouse model is free from a negative selection marker or other external insertions, which will be useful in future studies aimed at elucidating the multi-functional and physiological roles of PLAC8 in various diseases, without interference from exogenous foreign DNA.

• Journal of The Korean Association of Information Education
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• v.19 no.4
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• pp.509-516
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• 2015

As the interest in Education for Sustainable Development (ESD) increases worldwide in and around UNESCO, the aids for IT equipment and the donations for educations to developing countries are being active. Through Powerpoint-based teaching and learning contents simply authored by teachers, Mischief can be used to help many children in self-driven class activities. In this paper, we developed an additional program to control mouse pointers of Mischief with image files, size, and speed factors. Then we applied them to elementary students for enhancing their interest and participation to class activities.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.1
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• pp.7-12
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• 2000

The expression of the mouse $\alpha-amylase$ gene in the methylotrophic yeast, P pastoris was investigated. The mouse $\alpha-amylase$ gene was inserted into the multi-cloning site of a Pichi a expression vector, pPIC9, yielding a new expression vector pME624. The plasmid pME624 was digested with SalI or BglII, and was introduced into P. pastoris strain GSl15 by the PEG1000 method. Fifty-three transformants were obtained by the transplacement of pME624 digested with SaiII or BglII into the HIS4locus $(38\;of\;Mut^+\;clone)$ or into the AOX1 locus $(15\;of\;Mut^s\;clone)$. Southern blot was carried out in 11 transformants, which showed that the mouse $\alpha-amylase$ gene was integrated into the Pichia chromosome. When the second screening was performed in shaker culture, transformant G2 showed the highest $\alpha-amylase$ activity, 290 units/ml after 3-day culture, among 53 transformants. When this expression level of the mouse $\alpha-amylase$ gene is compared with that in recombinant Saccharomyces cerevisiae harboring a plasmid encoding the same mouse $\alpha-amylase$ gene, the specific enzyme activity is eight fold higher than that of the recombinant S. cerevisiae.

• BMB Reports
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• v.52 no.4
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• pp.271-276
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• 2019

Zingerone (ZGR), a phenolic alkanone isolated from ginger, has been reported to possess pharmacological activities such as anti-inflammatory and anti-apoptotic effects. This study was initiated to determine whether ZGR could modulate renal functional damage in a mouse model of sepsis and to elucidate the underlying mechanisms. The potential of ZGR treatment to reduce renal damage induced by cecal ligation and puncture (CLP) surgery in mice was measured by assessment of serum creatinine, blood urea nitrogen (BUN), lipid peroxidation, total glutathione, glutathione peroxidase activity, catalase activity, and superoxide dismutase activity. Treatment with ZGR resulted in elevated plasma levels of BUN and creatinine, and of protein in urine in mice with CLP-induced renal damage. Moreover, ZGR inhibited nuclear $factor-{\kappa}B$ activation and reduced the induction of nitric oxide synthase and excessive production of nitric acid. ZGR treatment also reduced the plasma levels of interleukin-6 and tumor necrosis $factor-{\alpha}$, reduced lethality due to CLP-induced sepsis, increased lipid peroxidation, and markedly enhanced the antioxidant defense system by restoring the levels of superoxide dismutase, glutathione peroxidase, and catalase in kidney tissues. Our study showed renal suppressive effects of zingerone in a mouse model of sepsis, suggesting that ZGR protects mice against sepsis-triggered renal injury.

• Molecules and Cells
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• v.46 no.2
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• pp.120-129
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• 2023

Recent technical advances have enabled unbiased transcriptomic and epigenetic analysis of each cell, known as "single-cell analysis". Single-cell analysis has a variety of technical approaches to investigate the state of each cell, including mRNA levels (transcriptome), the immune repertoire (immune repertoire analysis), cell surface proteins (surface proteome analysis), chromatin accessibility (epigenome), and accordance with genome variants (eQTLs; expression quantitative trait loci). As an effective tool for investigating robust immune responses in coronavirus disease 2019 (COVID-19), many researchers performed single-cell analysis to capture the diverse, unbiased immune cell activation and differentiation. Despite challenges elucidating the complicated immune microenvironments of chronic inflammatory diseases using existing experimental methods, it is now possible to capture the simultaneous immune features of different cell types across inflamed tissues using various single-cell tools. In this review, we introduce patient-based and experimental mouse model research utilizing single-cell analyses in the field of chronic inflammatory diseases, as well as multi-organ atlas targeting immune cells.

• Proceedings of the Optical Society of Korea Conference
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• 2008.02a
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• pp.157-158
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• 2008