• 한국잠사곤충학회지
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• 제39권2호
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• pp.174-179
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• 1997

The larval head capsule width, larval weight and length of mulberry longicorn beetle, Apriona germari Hope were measured when the larvae were exuviated in artificial diet rearing. The larval head capsule width from the 1st to the 12th instar was ranged from 0.12 to 0.69 cm, and growth ratio of each instar was significantly high between the 1st and the 2nd instars. The fitness to Dyar's law for the larval head capsule width was 87.6%. When the logarithum of the larval head capsule width is plotted against the number of instars, the calculated regression line was LogY=0.12086+0.06998X and Dyar's constant was 1.18. The larval weight was increased with larval developmental stages, and the coefficient of variation of larval weight was apparently high. But the calculated regression line was LogY=-0.91592+0.25959X and Dyar's constant was 1.25. The growth ration of the larval length was clearly high between the 2nd and the 3rd instars, and that of larvae from the 4th instar was decreased. The calculated regression line was LogY=-0.16932+0.09841X and Dyar's constant was 1.25. In conclusion, our results suggested that the larvae growth of mulberry longicorn beetle appeared to be highly related in the larval head capsule width, larval weight and length.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권2호
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• pp.161-163
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• 2000

To assess effects of cold treatment on pupation and emergence of the mulberry longicorn beetle, Apriona germari, the larvae, which ceased feeding, were exposed to three different temperature regimes (2.5, 5 and 7.5$^{\circ}C$) and preservation periods (50, 80, and l10 days). The results indicated that pupation and emergence rates of A. germari were highest at $7.5^{\circ}C$ for 110 days, 40.7% and 37.0%, respectively, demonstrating that the effect of cold treatment on metamorphosis was approximately 3 to 4 fold stronger than that of nontreatment.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권2호
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• pp.113-117
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• 2005

A chymotrpsin gene homologue was cloned from the mulberry longicorn beetle, Apriona germari. The A. germari chymotrypsin cDNA contains an ORF of 950 nucleotides capable of encoding a 283 amino acid polypeptide with a predicted molecular mass of 29151 Da and pI of 9.38. The A. germari chymotrypsin has conserved six cysteine residues and active triad formed by His, Asp and Ser. The deduced amino acid sequence of the A. germari chymotrypsin cDNA was closest in structure to the Anthonomus grandis chymotrypsin. Northern blot analysis revealed that A. germari chymotrypsin showed the midgut-specific expression.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권2호
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• pp.137-141
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• 2000

To determine effects of temperatures and photoperiods on larval development of the mulberry longicorn beetle, Apriona germari, the larvae were reared at various rearing temperatures and under the various photoperiods on an artificial diet. The larval period of A. germari was extended as long as the temperature was lowered. Also the larval development in terms of length and weight of larvae was increased. However, survival rate during larval stage significantly decreased at 15$^{\circ}C$ and $20^{\circ}C$ than at $25^{\circ}C$ and $30^{\circ}C$. The results indicated that the favorable temperature for artificial diet rearing of A. germari fell at least above $25^{\circ}C$ constantly. In photoperiod conditions, survival rate and larval development for A. germari were obviously most effective under a photoperiod of 14L:10B. As a result in artificial diet rearing of a. germari at $25^{\circ}C$ and under a photoperiod of 14L:10D was mostly favorable in terms of larval development and period.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.149-153
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• 2004

Here we report the molecular cloning of a LIM protein cDNA of the CRP (cysteine-rich protein) family from the mulberry longicorn beetle, Apriona, geramri. The A. germari LIM protein cDNA contains an open reading frame of 276 bp encoding 92 amino acid residues with a calculated molecular weight of approximately 10 kDa. The A. germari LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in cysteine-rich protein family 1 (CRP1). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the A. germari LIM protein cDNA showed 81 % identity to both Bombyx mori muscle LIM protein (Mlp) and Drosophila melanogaster Mlp60A and 77% to Epiblema scudderiana Mlp. Northern blot analysis showed that A. germari LIM protein is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body.

• 한국잠사곤충학회지
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• 제37권2호
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• pp.167-171
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• 1995

딱정벌레목 천공성 해충인 뽕나무 하늘소(Apriona germari)의 효과적인 방제를 위하여, 뽕나무 하늘소 이병충으로 부터 곤충병원 사상균을 분리하고, 위상차 현미경 및 주사전자현미경으로 관찰하였으며, PCR(polymerase chain reaction)을 이용하여 동정하였다. 뽕나무 하늘소 이병충으로 부터 분리된 곤충병원 사상균은 현미경 관찰 결과 Beauveria속의 전형적인 형태적 특성을 나타냈다. 따라서 이들의 용이한 동정을 위하여 PCR primer(5'-ACG GGC GCT C-3')를 이용한 RAPD(random amplification of polymorphic DNA) 방법으로 분석하고, B. bassiana와 B. brongniartii의 PCR 산물을 전기영동한 결과 DNA 표식자로 이용이 가능하였다. 이상의 결과로서 본 실험에서 분리·명명된 SFB-1A는 B. bassianifh, SFB-1A는 B. bassiana로, SFB-3A는 B. brongniartii로 동정되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.63-68
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• 2002

A cDNA encoding a putative member of cathepsin B of the thiol pretense superfamily was cloned from a cDNA library of the mulberry longicorn beetle, Apriona germari. Sequence analysis of the cDNA encoding the cathepsin B of A. germari (AgCatB) revealed that the 972 bp cDNA has an open reading frame of 324 amino acid residues. The deduced protein sequence of the AgCatB showed high homology with cathepsin B of the insects, Bombyx mori (47.3% amino acid identity), Helicoverpa armigera (46.6%) and Sarcophaga peregrina (45.6%), and the lowest homology with Aedes aegypti (33.2%). The AgCatB contains six disulfate bonds typical for cysteine pretenses. The three amino acid positions Cys-109, His-267, and Asn-287 which are conserved, active sites characteristic for cathepsin B, were also found. Phylogenetic analysis further confirmed that the AgCatB has a close relationship with that of B. mori, H. armigera and S. peregrina.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.157-159
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• 2003

We have cloned and sequenced the cDNA coding fur a cellulase from the mulberry longicorn beetle, Apriona germari, with the polymerase chain reaction. And then we have constructed the recombinant plasmid vector for Bombyx mori transfomation experiment. (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.161-166
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• 2001

The storage proteins of the mulberry longicorn beetle, Apriona germari Hope, were purified and characterized. Three kinds of storage protein (SP1, SP2 and Sp3) were purified from the last instar larval hemolymph of A. germari by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. The SP1, SP2 and SP3 have a native molecular weight of 480, 440 and 420 kDa, respectively. In the SDS-polyacrylamide gel electrophoresis analysis, these storage proteins are composed of a single protein subunit with molecular weight of 90, 85 and 80 kDa, respectively. This result showed that the storage proteins are hexameric protein. The SP1 and SP2 were stained with Schiffs reagent, but SP3 was not stained. It can be assumed that SP1 and SP2 are glycoprotein. Western blot analyses using the each of polyclonal antiserum against purified SP1, SP2 and SP3 showed that the three antibodies reacted with the each of SP bands, respectively. Also, antibodies against SP1 and SP3 cross-reacted with the SP3 and SP1, respectively. However, SP2 was not cross-reacted with these two antibodies. Also, antiserum against SP2 did not cross-reacted with the SP1 and SP3.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.121-126
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• 2001

A cDNA encoding a cathepsin D homologue was cloned from a cDNA library of the mulberry longicorn beetle, Apriona germari. Sequence analysis of the cDNA encoding the cathepsin D homologue of A. germari revealed that the 1,158 bp cDNA has an open reading frame of 386 amino acid residues. The deduced protein sequence of the A. germari cathepsin D homologue shows high homology with cathepsin D in insects, Aedes aegypti (68.2% amino acid similarity) and Drosophila melanogaster (67.2% amino acid similarity). Two aspartic residues and six cystein residues in the A. germari cathepsin D homologue are present at identical locations in all of the other catepsins D. Unlike cathepsins D in two insect species, A. gemari cathepsin D homologue appears to have two putative glycosylation sites, rather than one. Phylogenetic analysis revealed the A. germari cathepsin D homologue is more closely related to insect cathepsins D than to the other animal cathepsins D. Northern blot analysis suggests that A. germari cathepsin D homologue gene is expressed in most if not all, body tissues.