• Archives of Pharmacal Research
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• v.21 no.5
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• pp.555-558
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• 1998

chondroitin sulfates were isolated from the mud snail. For the quantitative analysis of enzymatic digestion products of isolated chondroitin sulfates, strong anion exchange-high performance liquid chromatography (SAX-HPLC) was performed. by the action of chondroitinase ABC, three unsaturated disaccharides$ 2-acetamide-2-deoxy-3-O-({\beta}-D-gluco-4-enepyranosyluronic acid)-D-galactose $$({\Delta}Di-OS), $2-acetamide-2-deoxy-3-O-({\beta}-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose ({\Delta}Di-6S) and 2-acetamide-2-deoxy-3-O-({\beta}-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose ({\Delta}Di-4S)$ were produced from the mud snail chondroitin sulfates. The analysis showed that relative proportion of ${\Delta}Di-OS/{\Delta}Di-6S/{\Delta}Di-4S$ was 58.7/3.1/38.2. The immunomodulating activity of chondroitin sulfate was examined by cell proliferation assay and these results suggest that it might be a immunosuppressant.

• Korean Journal of Environmental Agriculture
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• v.18 no.4
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• pp.299-303
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• 1999

A set of surveys was performed at Chungchongbuk-Do in 1997 in order to analyze energy related in rice production. Four cases in rice farming were surveyed : traditional farming, no chemical farming, duck farming, mud snail farming. The farmer in traditional fanning has used chemical fertilizers and chemicals such as general farmers. The farmer in no chemical farming has used chemical fertilizers and manure but has not use chemicals. The farmer in duck farming has not used chemical fertilizers and chemicals but brought up duck in paddy. The farmer in mud snail has not used chemical fertilizers and chemicals but brought up mud snail in paddy. The animals n paddy as like duck and mud snail not only eat weeds and insect but also input fertile material by excretion. The results of energy analysis are as follows 1. In view of energy, the duck farming was the most efficient of four cases. The mud snail farming was less efficient than the duck farming, the traditional farming was less efficient than the mud snail farming, the na chemical farming was less efiicierrt than the traditional farming. 2. Relatively to amount of product, non renewable energy was used more in traditional farming than other cases and renewable energy was used more in no chemical farming than other cases. 3. The reason of low energy efficiency in the no chemical farming was to input fertilizers and manure so much. So to input nutrients excessively in order not to use chemicals can make rice farming to be inefficient in a view of energy. 4. The farming to bring up animals in paddy was more sustainable than other cases because input of non renewable energy could be decreased. But in order to make it to be more sustainable, should be developed cultivation method to decrease input of non renewable direct energy such as fossil fuel and electricity .

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.191-192
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• 2011

Pulmonate snail, Onchidium hongkongensis from muddy flat of Muan-gun was recorded as new to the Korean molluscan fauna. Shell-less body light-gray or reddish-gray in colour, oval shaped with numerous pustules on dorsal surface usually covered with mud. Black pair of tentacles with terminal eyes. Including the new record in this study, the family Onchidiidae 2 genera and 2 species in the Korean waters.

• The Korean Journal of Malacology
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• v.8 no.1
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• pp.21-28
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• 1992

Field investigations were made in 1991 and 1992 in Kwangju and the western and central Chonnam area to determine the distribution of Lymnaea viridis, the intermediate host of Fasciola hepatica, and to study their life history during the different seasons. It had a wide distribution, existing all over the places, and the principal habitats of the snail were rice paddies. The life cycle was determined by making frequent visits to a series of habitats. The snsils began to produce egg masses in April, and egg production was the highest in May and June. Egg laying was continued but decreased in summer. There was sudden decrease in egg production in October when the temperature was below 2$0^{\circ}C$. The number of snails in July. Under the unfavourble conditions the snail either crawle up on the snail to aestivation. At low temperature below 8$^{\circ}C$ the snails hibernated inthe mud of the habitat. It was found in some habitats thao the snails were loving together with L. auricularia and Physa s pp. which indicates the neccessity of differentiation among them.

• The Korean Journal of Food And Nutrition
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• v.11 no.4
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• pp.431-436
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• 1998

The present study is concerned with the bacteriological examination of retort pouched loach soup and soybean paste soup containing mud snail. It was found that before sterilization, viable cell counts (c.f.u/ml) in their media were for the loach soup 3.80${\times}$106 on TSA, 2.27${\times}$102 Endo agar, 5.20${\times}$102 on SS agar. With the culture media, SDA and TCBS, no microorgarnisms were isolated from the both soups. Sixteen species of microorgarnisms were identified for the unsterilized soups. In the loach soup on TSA, B. pantothenticus was the dominant species, followed by S. dysenteriae, C. sporogenes and some others were also identified, such on TSA, B. marinus was the dominant species, followed by S. aureus, S. saccharolytics and P. tetradius, S. adorifera, E. ictahuri, E. gergoviae, E. coli were also identified. On Endo agar, the two soups showed a similar bacteriological pattern, in which entrobacterium such as E. gergoviae and E. coli were identified. Particularly K. subsp. rhinoscleromatis for the loach soup. On SS agar, S. ficaria and P. prevotii in the loach soup, S. ficaria and P. tetradius in the soybean paste soup were identified respectively. Bacteriological examination was also carried out for the spoiled retort pouched soup in the market, in which thirteen microorgarnisms were isolated and its pattern almost similar to that before sterilzation. They were B. pantothenticus, S. dysenteriae, C. sporogenes, P. gergoviae, E. ictaluri, S. ficaria, K. subsp. rhinoscleromatis in the loach soup and B. marinus, S. aureus, S. saccharolytics, P. tetradius, E. ictaluri, S. ficaria, S. adorifera in the soybean soup.

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.09a
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• pp.72-90
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• 2001

Environment agriculture carried out from 1999 in Daeho reclaimed saline area, located in central east cost of Choongnam Korea, resulted in dramatic reduction of amount of nitrogen fertilizer and application times and amount of pesticides. The ecological status of Daeho reclaimed saline area was considered to still very sound from the results of ecological survey on flora and fauna. However, it was desirable to adapt precision agriculture for production of high eating quality of rice and preservation of Daeho ecosystem. Especially, precise application of nitrogen and phosphorus fertilizer was recommendable for prevention of water pollution in environment rice cultivation by duck or mud snail or crab. The bioefficacy of Scirpus maritimus and Echinochloa crus-galli in paddy field of environment rice cultivation by duck or mud snail or crab in Daeho reclaimed saline area was revealed very low. Therefor, it was concluded that the pre-measures of reduction of natural weed population were necessary for successful environment agriculture. The most desirable and ideal environmentally sound agriculture in Daeho reclaimed saline area was performance of crop rotation, introduction of legume crops and green manure crops, and also, simultaneous management of crop production and animal husbandry for smooth flow of energy cycle within the closed Daeho ecosystem.

• Parasites, Hosts and Diseases
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• v.27 no.3
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• pp.203-212
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• 1989

The development of Echinestcma cinetorchis in several snail species reared in laboratory aquaria was observed. The eggs from adult cukes collected from the intestine of rats were cultivated to miracidia, and exposed to Hippeutis sp. snails. Observations were made for cercarial shedding from the exposed snails. The cercariae shed from the snails were again exposed to several species of fresh water snails in order to observe metacercarial formation in the snails and their infectivity to final hosts. The results obtained in this study were as follows: 1. Twenty miracidia were exposed to each snail of Hippeutis sp. About 58.3% of the above snails (7 out of 12) were dead before shedding the cercariae, anti the remainder shed the cercariae for a period of 7 to 9 days before death. 2. Cercarial shedding from the infected snails started from the 25th day after the exposure to mi.acidia, and the total number of cercariae shed per snail was 684 in average (range; 482-904). 3. The sixte of refine developed in the infected Hippeutis sp. snails was 1$, 242{\times}214{\;}{\mu}m$ in average, and the number of rediae per snail was 350 in average (range; 120-510). 4. About 40 to 50 cercariae shed from the Hippeutis sp. snails were each exposed to several species of snails reared in the laboratory. The metacercarial formation was confirmed by dissecting the infected snails, 12 to 16 days after the infection. The infectivity to each snail species was 100% in Hippeutis sp. (recovery rate; 56.7%) and Radix auricuzaria coreana (recovery rate; 66.4%), 66.7% in Physa acute (recovery rate; 37.5%), and 50% in Cipangopaludina sp. (recovery rate; 8.0%), respectively. 5. The swimming cercariae attached first at the cephalo-podial part of the snails and then migrated to the mantle, internal organ s and hemocele areas to form the metacercariae. 6. Adult worms of E. cinetorchis were obtained from the rats infected with the metacercariae encysted in the experimental snails. Summarising the above results, it is suggested that the mud-snail (Cipangopaludina sp.) may play an important role as a source of human infection with E. cinetorchis in Korea, and that several species of fresh water snails are involved in the life cycle as a second intermediate host.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.109-115
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• 2007

This study was carried out to determine occurrence and damage of golden apple snail, Pomacea canaliculata on water seeding area in Jeonnam province. The density of golden apple snail maintained hlgh until October 2003, but quickly decreased from November. They overwintered only in a portion of the water canals but could not find in dried paddy field. They reproduced from May and June in the canal water and paddy field, respectively. Feeding time of golden apple snail on rice seedlings was 235.2 seconds, feeding length of that was 8.4cm. When golden apple snail fed rice seedlings, the stem remain only 2.5cm from areal part which could not recover. The more density of golden apple snail, the fester feeding rate. Density and number of eggs laid of golden apple snail after overwintering was higher on bank around of paddy field than on middle of that. The percent of shell size after over wintering was higher in small (below 25mm) than big (over 36mm). But in case of Boseong area, the slze was reverse because the soil has soft mud. The survival rate of overwintering golden apple snail was lower than that of breeding. They did not move when the temperature changed rapidly, but the temperature increased they start to move. They dead almost below $-3^{\circ}C$. The damage of rice by golden apple snail in the direct water seeding was 20%. In machine transplanting, rice damaged only fore-end of leaf and recovered subsequently. When golden apple snail release in direct water seeding field, the percent of damage was 5.6% in immediately release plot. The later release, the lower damage.

• Korean Journal of Veterinary Research
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• v.32 no.2
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• pp.239-243
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• 1992

In the present experiment, the blue-green algae, the principal food of the vector snail of Fasciola hepatica were cultured. Mud from good natural habitats was sterilized, made slopes, inoculated with algae from the habitats and maintained in a saturated atmosphere. Under the fluorescent-mercury lamp(100W) at about $20^{\circ}C$ the algal growth was optimal, taking 8 days to fully grow and to be ready for feeding the snails. The algae collected from the habitats and cultured in the laboratory were mainly green.

• Korean Journal of Veterinary Research
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• v.31 no.4
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• pp.515-518
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• 1991

A field study of Lymnaea viridis, the intermediate host of Fasciola hepatica, was carried out in the eastern Chonnam area. The snail was distributed all over the place. Their major habitats were rice paddies (60.8%), followed by brooks (23.2%), irrigation canals (8.6%), and drains (5.6%). Other minor habitats included natural ponds (1.5%), lakes (0.4%), and rivers (0.1%). L. viridis was living in wet clay soil, occasionally entering water. The snails discharged egg masses when the temperature was $15{\sim}20^{\circ}C$, and the number of the snails was the highest late in October. At $9^{\circ}C$ the snails were not found on the mud: they were present deep in water or at the base of rice stubbles. These hibernation sites were discussed with the epidemiology of fascioliasis.