• Korean Journal of Ichthyology
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• v.17 no.3
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• pp.167-172
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• 2005

The flat-headed goby, Luciogobius guttatus, inhabits tidepools and river mouths, and stays under stones on the dried bottom for the duration of the low tide. To know the relationship of its respiration and habit in this fish, the epidermis of five appendages was observed. The epidermis has three layers: the outermost layer, middle layer and stratum germinativum. The outermost layer is composed of polygonal cells or rather flattened cells, and mucous cells. The unicellular mucous cells showing acid mucopolysaccharides are 11.1 to $16.1{\mu}m$ in mean height and in one or two rows. The middle layer consists mainly of large epidermal cells that are swollen by adjacent epidermal cells and arranged in a web-shaped structure. The swollen cells are 12.3 to $15.2{\mu}m$ in mean height and arranged in one to 11 layers. Since the swollen cells occupy the entire height of the epidermis, the epidermis is thick. A large number of blood capillaries are present just below the stratum germinativum. Taste buds are distributed at intervals on the surface of the epidermis. Based on these epidermal strucures, it is likely that L. guttatus utilizes cutaneous respiration in a dual respiratory systems.

• Korean Journal of Ichthyology
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• v.28 no.1
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• pp.28-34
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• 2016

The anatomy and histology of the olfactory organ in Favonigobius gymnauchen was investigated using a stereo microscopy, light microscopy and scanning electron microscopy. The paired olfactory organs in the dorsal snout are set in between the upper lip and the eyes. These organs are composed of two openings (anterior nostril with a tubular structure and posterior nostril), a single olfactory cavity, two nasal sac (ethmoidal and lacrimal sacs), olfactory nerve and olfactory bulb. The distributional pattern of the sensory epithelium is a only one type (continuous type). This epithelium is made up of the receptor cell, supporting cell and basal cell. The receptor cell has a only one type (ciliated receptor cell with 3~4 cilia). The non-sensory epithelium is built of the stratified epithelial cells and has mucous openings on the surface. Such an olfactory organ in F. gymnauchen may be considered to reflect its ecological habitat as a shallow water or tidal pool in the coastal zone.

• Applied Microscopy
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• v.37 no.1
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• pp.23-33
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• 2007

The lacrimal gland are compound tubule-acinar glands. The main lacrimal function is the production of the aqueous layer, the thickest and major constituent of the precorneal tear film. The lacrimal gland also has an important function in the defense system of the ocular surface, forming a part of the conjunctival-associated hymphoid tissue. The ultrastructural characteristics of the lacrimal gland of the rabbit were described. The lacrimal tissues of rabbits were processed through the conventional techniques for transmission electron microscopy. The secretory portions consisted of three cell types: 1. Serous cells with electron dense secretory granules. 2. Seromucous cells containing variable moderately electron dense secretory granules with flocculent material. 3. Mucous rolls containing mucous secretory granules. The serous cells were situated at the basal portion of acini, and they contained electron dense granules of variable densities and sizes. The seromucous cells contained a few protein secretory granules and more mucous secretory granules. The mucous cells contained even fewer protein secretory granules and exclusively mucous secretory granules. The epithelium of the intralobular ducts showed secretory granules, junctional complexes, and large basolateral intercellular spaces with lateral folds. These study might be helpful in determining inter-relationships, similarities and differences among the orbital glands of various physiological or pathological conditions.

• Animal cells and systems
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• v.7 no.1
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• pp.43-47
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• 2003

The skin of the eel goby, Odontamblyopus lacepedii, consists of epidermis, dermis and subcutis. The epidermis has three layers: the outermost layer, middle layer and stratum germinativum. The outermost layer is composed of polygonal cells or rather flattened cells, and mucous gland cells of acid mucopolysaccharides. The middle layer consists mainly of swollen small or voluminous epidermal cells and shows a web-shaped structure. The thickness of the epidermis depends on the various sizes and the number of layers of the swollen cells. Well-developed lymphatic spaces containing lymphocytes exist in the stratum germinativum and small scales are embedded in the dermis. A large number of blood capillaries are present just below the basement membrane, and a definite area giving AB and PAS positive was present between the basement membrane and scales, Taste buds ave distributed on surface of the epidermis at intervals. Considering the structural features of the skin, it may be considered that O. lacepedii is more likely to be related to cutaneous respiration as a dual respiratory system.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.131-136
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• 1994

In the present study, in order to make an in vitro model of gastric mucosa for physiological and pharmacological studies, we established two immortalized gastric surface mucous cell lines (GSM06 and GSM10), which produce periodic acid-Schiff (PAS)-and concanavalin A (Con A)-positive glycoproteins, from a primary culture of gastric fundic mucosal cells of adult transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen gene 〔1]. Gastric fundic mucosal cells were isolated as a modification of a previously described method for rats by Schepp et al. (2). The isolated gastric fundic mucosal cells were cultured in DME/F12 medium supplemented with 2% fetal bovine serum (FBS), 1% ITES (consisting of 2 mg/1 insulin, 2 mgg/1 transferrin, 0.122 mg/1 ethanolamine and 0.00914 mg/1 sodium selenite) and 10 ng/ml recombinant epidermal growth factor (EGF) in a collagen-coated culture dish. To remove fibroblastic cells from the culture, gastric mucosal cells were incubated in the culture medium containing dispase (25 U/ml) for 24 h. The cells, uncontaminated with fibroblastic cells, were then cloned by colony formation. In our series of three attempts, two cell lines (GSM06 and GSM10) have been established at last. The cells proliferated, attached to the dish ana grew until confluent monolayers were formed, and maintained tight contact with neighboring cells. Both GSM06 and GSM10 cells have now been in culture for more than 9 months with regular passaging. The either cell produced

• The Korean Journal of Malacology
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• v.12 no.1
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• pp.1-17
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• 1996

Morphological and histochemical characteristics of the cells in posterior tentacle antenna of Korean slug, Incilaria fruhstorferi were observed with light microscope. The epithelium of the posterior tentacle antenna was composed of supporting cells, sensory neurons and type-a clear cell. The columnar supporting epithelium was widely distributed in the posterior tentacle antenna, and the upper end of the cell was covered with acidic mucopolysaccharide. Nerve endings of the sensory neuron were distributed between type-a clear cells. It was usually located in tentacular knob, and the number of them gradually decrdased as close as tentacular stalk. Several cilia were observed on the nerve ending. Type-a clear cells were very brightly stained with all staining used, and the neutral mucous guanules distributed in the cytoplasm. Collar cells, type-b clear cell and various types of secrdtory cells distributed in the connective tissue. The collar cells were clustering in connective tissue, and the cytoplasm were filled with neutral mucous guanules. The cells and granules were stained with dark brown by silver nitrate stain. Type-b clear cells were irregular in shape and their cytoplasms were brightly stained wth many stains used. Ten types of secretory cells evenly distributed in the connective tissue and muscle layers of the posterior tentacle antenna. The five types of the secretory cells(A, B, E, J and L)seemed to secrete acidic mucopolysaccharide, and the other five type of the cell(C, D, F, H, and L)seemed to secrete neutral mucopolysaccharide. Muscular tissue composed of well-developed thick longitudinal muscle layers and thin circular muscle layers. Type-L secretory cells clustered only in muscular layers and they contained acidic mucopolysaccharides.

• Korean Journal of Ichthyology
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• v.19 no.3
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• pp.201-209
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• 2007

The fine structures and histochemical features on the integumentary system of the grass puffer, Takifugu niphobles were examined by means of the light and transmission electron microscopy. Integumentary surface of the grass puffer showed irregular folds in light microscope. The folds of the ventral region are more pronounced than those in the dorsal region. Integumentary system is composed of outer epidermal layer and inner dermal layer. The stratified epidermal layer consists of epithelia, mucous cells, club cells, granular cells and multivacuolar gland. Epithelial cells are classified into superficial, intermediated and basal cell, and free surface of superficial cell is covered with microridges. Glands of the epidermal layer are divided into unicellular and multicellular gland. Mucous cells of multicellular gland contains mucosal materials of neutral glycoprotein. Multivacuolar gland is composed of numerous vacuole cells of about $20{\mu}m$ in axial diameter. Vacuole cells contains a large central vacuole and are connected to another by many desmosomes. The mucous glands and multivacuolar glands are more abundant in ventral region than dorsal integument. The thickness of dermis is more three to five times than epidermis in ventral integument. The collagen fibers, fibrocytes, nerve cells, basal plate of spine and chromatophore are observed in the dermal layer of compact connective tissue.

• Journal of fish pathology
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• v.21 no.1
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• pp.45-56
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• 2008

This study was conducted to find out biological responses of bivalves exposed to organotin compound.The results of the study confirmed that tribultyltin chloride (TBTCl) induce reduction of survival rate andburrowing activity, and histopathological feature in the foot structure of the equilateral venus, Gomphinaveneriformis. The experimental period was 36 weeks. The experimental groups consisted of a control and 3TBTCl exposure groups (0.4, 0.6, 0.8 ym TBTCl L'). The survival rate and burrowing activity were record-ed daily. For histological analysis, foot tissues were fixed in Bouin' s fluid and then stained H-E stain, AB-PAS (PH 2.5) reaction and Masson's trichrome stain after having serially sectioned the tissue by paraffinmethod at thickness of 4-6 ym. The survival rate was not significantly different between the control andexposure groups for 20 weeks, but in 0.8 Um TBTCl L', it was on the decreased ever since the exposure. Theburrowing activity was not significantly different in the exposure group compared to the control up to 12weeks, but in 0.6 and 0.8 ym TBTCl L', it measured the lowest level after 20 weeks. The foot is composedof the epidermal layer, connective tissue, and muscular layer. The epidermal layer is composed of simplecolumnar, cuboidal epithelia and mucous cells. The cilia were well developed on the apical surface ofepithelium, Circular, longitudinal and transverse muscle bundle were well developed in the muscular layer.The majority mucous cells showed blue color (542c) when it subjected to AB-PAS (PH 2.5) reaction. Nohistopathological alterations in the foot were observed up to 12 weeks. After 20 weeks of exposure to 0.8 (anTBTCl L'', the foot samples of exposed G. veneriformis showed disappearance of cilia and striated borderpartially and extension of hemolymph sinus. The mucous cell increased in the marginal of foot. At 28-weekof exposure to 0.4 ym TBTCl L', it observed weekly acid (564c), neutral (264c) and mixed mucous cell. At36-week of exposure to 0.6 ym TBTCl L', it showed fragmentation of the muscle and collagen fiber bundle,and also diappearance of cilia on epithelia and edema of epithelium in 0.8 ym TBTCl L''.

• Applied Microscopy
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• v.35 no.2
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• pp.57-64
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• 2005

The ultrastructure and histochemical characteristics of the submandibular gland was examined in the big white-toothed shrew, Crocidura lasiura. A submandibular gland of Crocidura lasiura was a mixed gland composed of serous and mucous acinar cells. Secretory granules from the acini were discharged through the intercalated duct, the granular duct and the striated duct into the oral cavity. Serous and mucous acinar cells and granular duct cells had large amount of rough endoplasmic reticulum, free ribosome and prominent Golgi apparatus at the basal cytoplasm of the cell, and many granules at the apical cytoplasm. Oval type serous granules had a homogeneously pale round shape of bead at the center. Mucous granules were distinct from those of the other mammalian species having variety patterns with several dense bands into homogeneous pale matrix. A serous-like secretory granules and myelin-like body were observed in the cytoplasm and the lumen of granular duct cells. The myelin-like body is a characteristic structure only reported in the salivary glands of two shrews, Suncus murinus and C. dsinezumi. Striated duct cell had numerous well-developed mitochondria but secretory granule was not shown at all.

• The Korean Journal of Zoology
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• v.38 no.1
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• pp.26-37
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• 1995

뱀장어, Anguilla joponicu의 표피를 구성하는 주종 세포인 상피세포는 80남 정도의 많은 당김세사를 함유하고 있어서 표피의 골격 유지에 중요한 역할을 하고 있다 회유행동 특성에 의해. 성숙된 뱀장어는 바다로 나가게 되고 표피는 급격한 환경변화를 서게 되는데 그 현상들을 살펴보면 먼저 상해반응으로 세포 내의 파립 형질내세망의 내강이 확장되는 현상과 다양한 크기의 공포의 증가로 인해 상피세포들 사이의 공간이 확장되며 일부 세포에서는 괴사 또는 변성되는 형태인 다층층판구조를 갖기도 한다. 이에 대한 능동적 대처로 부착반쪽으로 모이는 당김세사들이 일정한 방향성을 갖게 되며, 상피세포 사이의 연접부위에 부착반의 수가 증가되며 미토콘드리아. 형질내세망 등 세포소기 관이 발달되고, 분비과립의 증가 등 분비양상이 증가되고, 능동적인 염배출과 연관된 핵상부의 중앙축을 따라 미토콘드리아 및 과립 형질내세망이 풍부한 세포도 나타났다. 이와 같은 변화는 염분농도의 증가에 따른 환경적요인에 의해 일어나는 상피세포의 기능적 방어기작이라고 사료된다.