• Animal cells and systems
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• 제12권2호
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• pp.85-91
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• 2008

Structure and mucosubstance histochemistry in the epidermis of five teleostean species, i. e., spotty belly, Agrammus agrammus, devil stinger, Inimicus japonicus, stone fish, Erosa erosa, cubed snailfish, Liparis tessellatus, and Japanese bluefish, Scombrops boops were investigated. The epidermis of five species studied is composed of three layers: superficial, middle, and basal layer. The superficial layer is comprised of rather flattened cells. Mucous cells, the type commonly found in fishes are completely lacking in the epidermis of devil stinger, stone fish, and cubed snailfish. The epidermis of devil stinger and stone fish have multicellular glands which do not have mucosubstances. The skin surface of them is covered with mucous layer. The superficial cells in the epidermis of devil stinger, stone fish, and cubed snailfish are mucus-secreting cells. The composition and the amount of the mucosubstances vary in species and body regions. The mucous layer on the skin surface and superficial epidermal cells of devil stinger contain a mixture of neutral and acidic(sulfated and nonsulfated) mucosubstances. In stone fish, the mucous layer has acidic(sulfated and non-sulfated) mucin and the superficial epidermal cells contain neutral mucin. In cubed snailfish, the type of epidermal mucosubstances is identified as a mixture of neutral and acidic(non-sulfated) mucin. The mucous cells of the epidermis in spotty belly and Japanese bluefish contain neutral mucin.

• Animal cells and systems
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• 제11권2호
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• pp.183-189
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• 2007

To investigate the properties of mucosubstances of the epidermis in various teleostean species, conventional histochemical stainings were used on the skin in five species of order Perciformes, i. e., yellowtail, Seriota quinqueradiat, striped beakperch, Oplegnathus fasciatus, brown spotted grouper, Epinephelus chlorostigma, sea chub, Ditrema temmincki and multicolorfin rainbowfish, Halichoeres poecilopterus. The following methods were used: periodic acid Schiff (PAS), alcian blue (AB) pH at 2.5, AB pH at 1.0, AB pH at 2.5-PAS, AB pH at 1.0-PAS, aldehyde fuchsin (AF) pH at 1.7-AB pH at 2.5 and high iron diamine (HID)-AB pH at 2.5. The epidermis of all five species consisted of three layers: superficial, middle, and basal layer. The superficial layer was comprised of rather flattened cells. In particular, the outermost layer of striped beakperch and middle layer of sea chub consisted of mucus-secreting cells. Mucous cells, the unicellular glands, were found in epidermis but varied in number in different body regions and species. Although there was a slight difference in the amount in various species and body regions, the secretory contents of the mucous cells in the five teleostean species contained acidic mucopolysaccharides. In yellowtail, striped beakperch, and multicolorfin rainbowfish, the property of mucosubstances was identified as sialomucin, while it was sulphomucin in brown spotted grouper and sea chub.

• Animal cells and systems
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• 제3권3호
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• pp.253-258
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• 1999

The present study describes histochemical properties of the mucosubstances in the pedal sole of abalone, in relation to two main additional functions known to date. Only neutral mucosubstances were demonstrated in the lateral epithelium of the pedal sole of the abalone, however, two distinct types of cells, one containing neutral and the other containing acidic mucosubstances, were identified in the ventral epithelium. The epithelial mucocytes distributed throughout both epithelial and subepithelial glands located in the ventral subepithelial region showed a mixture of neutral and acidic mucosubstances. However, acidic mucosubstances were found in larger amounts than neutral ones in epithelial mucocytes, and vice versa in subepithelial glands. Among these acidic mucosubstances, sulphated substances were dominant. Lesser amounts of carboxylated substances also occurred together with low levels of neuramic and sialic acid. Collectively, the notable morphological differences between abalone and other gastropoda were observed in the ventral surface of the foot, which showed a tall columnar epithelium, peripherally concentrated mucocytes, and subepithelial glands. Mucosubstances are presumably produced by these cells and may also play a role in clinging to some substances to support abalones in addition to the main functions of lubrication and protection.

• Applied Microscopy
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• 제31권4호
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• pp.325-331
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• 2001

돌가자미의 피부 상피층을 다층상피층으로 지지세포, 선세포 그리고 과립세포들로 구성된다. 상피층은 지지세포의 형태와 구조에 따라 표면층, 중간층 및 기저층으로 구분 할 수 있었다. 지지세포들의 세포질은 피질부와 수질부로 나누어지는데 피질부에는 미세섬유의 발달이 뚜렷하다. 점액세포들은 단세포선으로 상피의 표면층과 중간층에서 관찰된다. 점액세포의 점액물질은 중성이며, carboxylated mucosubstance의 당단백 질로 확인되었다. 곤봉상세포는 세포질에 잘 발달된 활면소포체와 골지체를 가진다. 과립세포는 주로 중간층과 기저층에 존재하고, 세포질은 막을 가진 전자밀도가 높은 과립들이 차지한다. 색소세포는 세포질에 존재하는 함유물의 전자밀도에 따라 세 종류로 구분할 수 있었으며, 색소세포 근처에서 신경종말을 관찰할 수 있었다.

• Parasites, Hosts and Diseases
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• 제28권3호
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• pp.155-160
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• 1990

우리 나라에서만 인체 감염이 확인된 장흡충 Fibricole seoulensis의 특징적 구조인 tribocytic organ의 기능을 파악하는 연구의 일환으로, 이 구조물과 표피의 조직화학적 성질을 밝히기 위해 특수 염색을 시행하였다. 충체 의 단면을 H&E 염색, 탄수화물, mucosubstance, amylo:임 및 교원질에 대한 특수 염색 및 alkaline phospha- tase에 대한 면역 조직화학 염색을 시행, 관찰하였다. Tribocytic organ의 표피, 분비물 및 선세포는 PAS 및 double bridge PAP에 양성이었다. 충체의 표피 역시 같은 염색에 양성이었다. 그러므로 F. seoulensis에서 tribocytic organ의 표피, 분비물 및 선세포와 충체의 표피는 neutral mucopolysaccharide를 함유하고 있으며 tribocytic organ의 표피, 분비물 및 충체의 표피는 alkaline phosphatase 활성도가 있음을 알 수 있었다. 이러한 구조들은 충체의 분비 기능 및 숙주 점막에의 부착 등에 관여할 것으로 생각된다.

• 대한수의학회지
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• 제28권2호
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• pp.261-270
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• 1988

This study was performed to investigate the effect of gestation on the histological and histochemical changes of the uterine cervix of the native Korean cattle. A total of 110 cows obtained from the Chonhuk abattoir were divided into six groups. The 1st group was of 9 non-pregnant cows and 101 singleton pregnant cows were grouped into 5 groups from pregnant I to pregnant V according to gestation periods by means of crown rump length measures. For light microscopy the tissues were fixed in 10% neutral formalin and processed routinely for paraffin sections The $6{\mu}m$ sections were taken and stained with H-E, Alcian blue pH 1.0, Alcian blue pH 2.5, Alcian blue pH 2.5/PAS, PAS reaction, toluidin blue, and trichrome. The results obtained were as follows: 1. The cervical lengths and widths were increased in relation to advancing gestation. 2. The cervical folds of the pregnant groups were increased and complicated with many branches, and the cervical muscosal epithelia were increased in according to advancing gestation. 3. As advancing gestation, the tunics muscularis of cervix was increased following moderate distribution of fibroblast and vascularity, meanwhile decreased mast cells were found. 4. The cervical mucosubstance was composed of mixed mucopolysaccharides; the acid mucus was increased from the early pregnant state but the neutral mucus was found after pregnant IV, and the mucus was stained deeply in each staining in the fold cavity in according to gestation state.

• Applied Microscopy
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• 제34권2호
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• pp.83-93
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• 2004

붉은쏨뱅이의 피부 상피층은 다층상피층으로 지지세포, 선세포, 과립세포 그리고 mitochodria-rich cell들로 구성된다. 상피층은 지지세포의 형태와 구조에 따라 표면층, 중간층 및 기저층으로 구분할 수 있다. 점액세포들은 단세포선으로 상피의 표면층과 중간층에 분포하며, 산성의 비황화 뮤코다당류로 구성된 점액을 가진다. 곤봉상세포는 세포질에 잘 발달된 중심공포, 조면소포체 및 골지체를 가진다. 과립세포는 표면층에 존재하며, 전자밀도가 높은 과립을 가진다. mitochodria-rich cell의 주변부 세포질에는 미세섬유 다발이 발달되어 있으며, 수질부에는 다수의 관상 미토콘드리아를 가진다. 색소세포는 세포질에 존재하는 함유물의 전자밀도에 따라 세 종류로 구분할 수 있다.