• Title/Summary/Keyword: Mucosal healing

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Buccal Mucosal Ulcer Healing Effect of rhEGF/Eudispert hv Hydrogel

  • Park, Jeong-Sook;Yoon, Joon-Il;Li, Hong;Moon, Dong-Cheul;Han, Kun
    • Archives of Pharmacal Research
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    • v.26 no.8
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    • pp.659-665
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    • 2003
  • We have studied the effect of rhEGF on the buccal mucosal ulcer healing. rhEGF was rapidly degraded upon incubation with the hamster buccal mucosal homogenates; The degradation of rhEGF was significantly inhibited by sodium lauryl sulfate (SLS). Eudispert hv hydrogel and Polycarbophil 974P hydrogel were prepared for rhEGF delivery and their mucoadhesiveness was measured by the $Instron^R$ method. The mucoadhesive force of Eudispert hv was significantly greater than that of Polycarbophil 974P. Moreover, rhEGF in Eudispert hv hydrogel remained stable for about 2 months. To evaluate the ulcer healing effect of rhEGF, the buccal mucosal ulcer was induced in golden hamsters using acetic acid. At 24 h after administration of rhEGF/Eudispert hv hydrogel, the ulcerous area was decreased compared with rhEGF solution and, as a result, the curative ratio was $36.8\pm5.68$%. By the addition of SLS (0.5%) to Eudispert hv hydrogel, the curative ratio increased 1.5 times. The mechanism of the action was probably due to a combination of protection of the drug against proteases present in mucosa and prolongation of the release of rhEGF from the formulation at the site of action.

THE INDUCTIVE CAPACITY OF PRIMARY CULTURED ORAL MUCOSAL KERATINOCYTES IN SKIN WOUND HEALING OF ATHYMIC NUDE MICE (배양된 구강점막 각화상피세포가 누드마우스 피부 창상 치유에 미치는 효과)

  • Kim, Hyun-Sil;Kim, Nam-Hee;Kim, Jin;Cha, In-Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.30 no.4
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    • pp.308-315
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    • 2004
  • Aim: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes. Materials and methods: Thirty adult female nude mice weighing $20{\pm}2g$ were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto $1.5cm{\times}1.5cm$ sized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3. Results: In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes. Conclusion: We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.

AN EXPERIMENTAL STUDY ON SKIN AND MUCOSAL GRAFTS IN THE ORAL CAVITY (구강내 피부 및 점막이식에 관한 실험적 연구)

  • Choi, Jae-In;Kim, Jong-Won
    • The Journal of the Korean dental association
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    • v.22 no.1 s.176
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    • pp.49-55
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    • 1984
  • The objective of this study was to observe the healing of skin and mucosal grafts, comparing with the control sites. Six adult dogs were divided into two groups of three. Mucosal defects were created in all four quadrants, and the left side was used as a control. In the Group I animals, a split-thickness skin from the chest was grafted, and in the group II animals, a split-thickness mucosa from the buccal cheek was grafted into the mouth. Gross and microscopic examination was done at 3, 7, 14, 28, 42 days. The results were as follows. 1. Wound epithelization was accelerated in the supraperiosteal grafts. 2. After healing, the grafted skin was unnatural to the oral cavity, showing grayish-white color, hair, and sebaceous gland. But the mucosal grafts could not be discriminated from the adjacent mucosa. 3. the grafting on the cortical bone failed.

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Early Biologic Treatment in Pediatric Crohn's Disease: Catching the Therapeutic Window of Opportunity in Early Disease by Treat-to-Target

  • Kang, Ben;Choe, Yon Ho
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.21 no.1
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    • pp.1-11
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    • 2018
  • The emergence of mucosal healing as a treatment goal that could modify the natural course of Crohn's disease and the accumulating evidence showing that biologics are most effective in achieving mucosal healing, along with the success of early treatment regimens for rheumatoid arthritis, have led to the identification of early Crohn's disease and development of the concept of catching the therapeutic window during the early disease course. Thus, an increasing number of pediatric gastroenterologists are adopting an early biologic treatment strategy with or without an immunomodulator. Although early biologic treatment is effective, cost and overtreatment are issues that limit its early use. Currently, there are insufficient data on who will benefit most from early biologics, as well as on who will not need early or even any biologics. For now, top-down biologics should be considered for patients with currently known high-risk factors of poor outcomes. For other patients, close, objective monitoring and accelerating the step-up process by means of a treat-to-target approach seems the best way to catch the therapeutic window in early pediatric Crohn's disease. The individual benefits of immunomodulator addition during early biologic treatment should be weighed against its risks and decision on early combination treatment should be made after comprehensive discussion with each patient and guardian.

Treatment of intramuscular lipoma of tongue with enveloped mucosal flap design: a case report and review of the literature

  • Hur, Sung-Hwi;Lim, Jae-Seok;Choi, Sun-Gyu;Kang, Ji-Yeon;Jung, Ji-Hye;Lee, Eun-Young
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.42
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    • pp.38.1-38.7
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    • 2020
  • Background: Lipomas are benign soft tissue neoplasms of mature adipose tissue commonly occurring in the trunk or extremities. But, intraoral lipomas are rare entities which may be only noticed during routine dental examinations. Especially intramuscular lipomas on the tongue have been reported very rarely. In this study, we report a case of intramuscular lipoma on tongue, with a review of the literature from 1978 to 2019, providing data on age, gender, location, presenting symptoms, size, surgical methods, and recurrence. Case presentation: A case of intramuscular lipoma occurring in tongue region in a 65-year-old male is reported. Surgical excision is the mainstay of treatment for the lesion. In order to decrease the deformity and discomfort after the excision, we tried to modify surgical technique using enveloped mucosal flap. This technique provided more comfortable healing procedure on the operative site without recurrence. Conclusion: This is a rare case of large intramuscular lipoma on tongue. Surgical excision with enveloped mucosal flap design was performed to diminish postoperative raw surface and discomfort and a 24-month follow-up showed excellent healing without any recurrence. A case of intramuscular lipoma on tongue and relevant literature reviews are presented in this study.

CLINICAL STUDY OF FREE BUCCAL MUCOSAL GRAFT (유리협점막이식술의 임상적 연구)

  • Kim, Yong-Kack;Park, Hyung-Kuk;Kim, Ho;Kweon, Heok-Jin;Kim, Woong-Bee
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.17 no.3
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    • pp.214-219
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    • 1995
  • Free grafting of oral mucosa for minor oral reconstruction was first described by Propper in ridge extension surgery. Situation calling for mucosal grafting procedures may relate to periodontal surgery, minor and major preprosthetic surgery, implant surgery, reconstruction in deformity cases after trauma, congenital cleft, gross atrophy and ablative tumor surgery. In the cases of 9 patients with mucosal defect of intraoral or orbital cavity after wide excision of tumor, preprosthetic surgery, and orbitoplasty, full-thickness mucosal graft were used to close a large defect. Four patients received buccal mucosal graft for preprosthetic surgery or orbitoplasty, one patient had benign tumor and the others had malignant tumors located on the palate or upper alveolus. Buccal mucosal graft donor site morbidity and trismus were minimal and healing of surgical defect was satisfactory. So we present the case with review of literatures.

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Inducing re-epithelialization in skin wound through cultured oral mucosal keratinocytes

  • Kim, Hyun Sil;Kim, Nam Hee;Kim, Jin;Cha, In Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.39 no.2
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    • pp.63-70
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    • 2013
  • Objectives: The purpose of this study was to investigate the wound healing effect of primary cultured oral mucosal keratinocytes (OMKs) and to assess their roles in skin wounds. Materials and Methods: OMK labeled with BromodeoxyUridine were scattered onto $1.5{\times}1.5$ cm skin defects of adult female nude mice (OMK group, n=15). For the control, culture media were placed on the wound (control group, n=15). Mice in both groups were sacrificed at three days (n=5), one week (n=5), and two weeks (n=5), and histomorphometric and immunoblot analyses with keratinocyte growth factor (KGF), interleukin (IL)-6, and IL-$1{\alpha}$ antibody were performed for the biopsied wound specimen. To verify the effect of the cytokine, rhIL-$1{\alpha}$ was applied instead of OMK transplantation, and the OMK and control groups were compared with regard to re-epithelialization. Results: Histomorphometric analyses demonstrated faster re-epithelialization in the graft group than in the control group at the third day, first week, and second week. Newly forming epithelium showed maintenance of the histological character of the skin epithelium. The graft group showed superior expression of KGF, IL-6, and IL-$1{\alpha}$ protein, compared with the control group. Similar faster re-epithelialization was observed after treatment with rhIL-$1{\alpha}$ instead of OMK transplantation. Conclusion: We successfully confirmed that the graft of primary cultured OMKs promoted regeneration of skin defects. The mechanism of accelerated wound healing by primary cultured OMKs was attributed to inducement of cytokine expression as required for re-epithelialization.

Gastric Ulcer Healing Effects of Dioscorea japonica, Halloysite and Ostrea gigas Mixtures

  • Park, Jeong-Sook;Han, Kun
    • Natural Product Sciences
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    • v.15 no.2
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    • pp.96-100
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    • 2009
  • A novel gastric ulcer healing formulation, a mixture of Dioscoreae Rhizoma, Halloysitum Rubrum and Ostreae Testa (Dihaos), was examined for gastric ulcer healing effects. The effect of Dihaos was assessed in various gastric ulcer models in rats. Oral administration of Dihaos significantly reduced HCl-ethanol-induced gastric ulcers. Dihaos also significantly reduced gastric and duodenal ulcers induced by cysteamine. Ostreae Testa decreased secretion of gastric juice and increased the pH of gastric juice. Furthermore, the extracts of Dioscoreae Rhizoma affected the cell proliferation of MKN 74 cells. These results suggest that the healing effect of Dihaos on gastrohemorrhagic lesions results from its protective effect against acid secretion and proliferation of mucosal cells in induced gastric ulcers in rats.

Therapeutic Effect of Rebamipide on Ammonia-induced Gastric Mucosal Hemorrhagic Lesion in Rats

  • Huh, Keun;Kwon, Tae-Hyub;Kim, Soo-Kyun;Kim, Jin-Sook;Shin, Uk-Seob
    • Archives of Pharmacal Research
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    • v.21 no.1
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    • pp.1-5
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    • 1998
  • Rebamipide, 2-(4-chlorobenzoylamino)-3-[2(1H)-quinolinone-4-yl]-propionic acid, a novel antipeptic ulcer agent, has been reported to prevent various acute experimental gastric mucosal lesions and to accelerate the healing of chronic ulcers. Therapeutic effect of rebamipide was investigated with regard to the inhibitory effect on xanthine oxidase activity and type conversion of the enzyme which play a profound role in oxygen radicals generation system. Intraperitoneal administration of rebamipide at 60 mg/kg body weight reduced the xanthine oxidase activity, lipid peroxide content in ammonia induced hemorrhagic lesion. These results suggest that the therapeutic effect of rebamipide on gastric mucosal lesion may be in part due to the inhibitory activity of xanthine oxidase and type conversion rate of the enzyme.

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