• Korean Journal of Food Science and Technology
• /
• v.24 no.4
• /
• pp.326-329
• /
• 1992

Mucor sp. KCTC 8405P was cultivated in a jar fermentor for the production of fungal lipid containing ${\gamma}-linolenic$ acid with feeding the glucose solution periodically. The transition of the fungal growth into the mycelial phase from yeast-like growth was achieved by pH shift after the first two day of cultivation in the low pH medium and then lipid accumulation was accelerated until the seven day of cultivation, when the glucose in the culture broth was almost consumed. With the culture conditions applied in this experiment, biomass of 99.3 g/l by the dry cell weight and the total extractable lipid of 38.0 g containing 3.5 g/l ${\gamma}-linolenic$ acid were obtained.

• Microbiology and Biotechnology Letters
• /
• v.17 no.6
• /
• pp.568-573
• /
• 1989

As a way to determine the optimal culture conditions for the production of ${\gamma}$-linolenic acid by Mucor sp. KCTC 8405P, the influence of different carbon and nitrogen sources, initial pH, and C/N ratio of medium was investigated. Glucose was found to be the best carbon source in terms of lipid content and ${\gamma}$-linolenic acid yield. Ammonium sulfate and organic nitrogen sources such as urea and peptone resulted in relatively increased lipid and ${\gamma}$-linolenic acid production. The highest accumulation of lipid was obtained at a C/N ratio of 56.6 using glucose and (NH$_4$)$_2$SO$_4$ as carbon and nitrogen source, respectively. It was found that the lipid content increased significantly with increasing initial pH of medium up to pH 9.0. The influence of mixed carbon source on the ${\gamma}$-linolenic acid yield was also investigated. High accumulation of lipids, 315 mg/100 ml medium, and 13-14% of ${\gamma}$-linolenic acid content in the cellular lipid were obtained in a shaking culture containing 3% of glucose and 2% sodium acetate as carbon source and 0.1% of (NH$_4$)$_2$SO$_4$ as nitrogen source at pH 8.0.