• Korean Journal of Environment and Ecology
• /
• v.25 no.5
• /
• pp.799-813
• /
• 2011

Increasing mountain biking (MTB) in forest recreation areas is changing the behavioral patterns in the areas where hiking has been a dominant activity, and it may cause ecological and social impacts to the areas. This study examined hikers' perceived conflicts by MTB including the relationship between socio-demographic and visiting characteristics and perceived conflict. On-site survey was conducted in Mudeungsan Provincial Park (MPP), Korea, during September, 2010. Out of 388 hikers participated in the survey, 374 responses were used for data analysis. 30.2% of the respondents encountered or saw MTB (avg.: 1.7 times) in MPP. Overall, 77.1% of respondents thought MTB in MPP was a problem. 73.5%~83.2% of respondents perceived conflict by four major MTB behaviors (e.g., too fast running MTB in trails; MTB bikes occupying trails; MTB bikes passing by hikers too closely; MTB bikers doing unpleasant behaviors to hikers). Based on the type of conflict, 48.5~62.4% of respondents fell in the type of "social values conflict (SVC)", 11.1~32.4%, "interpersonal conflict (IC)", and 16.8~26.6%, "no conflict (NC)", respectively. Types of conflicts were related with sociodemographic (e.g., gender, age, education) and visiting characteristics (e.g., number of visits, experience of encountering MTB). Such relationships were nonexistent in most cases when the "experience of encountering MTB" was controlled.

• 한국체육학회지인문사회과학편
• /
• v.55 no.2
• /
• pp.95-110
• /
• 2016

The purpose of this research is to analyze and understand MTB(mountain bike) participants' life in subcultural perspective. This research was completed by 8 people live in Seoul, Gyeonggi and Incheon who regularly participated their activity and changed their job to MTB. Observe them by participation, in-depth interview, field notes and researcher's daily records were methods to collect these data. To accomplish this purpose of study, I chose ethnographic Research. As a result, feeling doubt of their previous life, be caught in unique fun of MTB that made them to think MTB leisure seriously as a job, experience of its professionalism they learnt, and changed their job voluntarily were majority opinions. After they changed their job, they seems to have weisure life, more enthusiastic, got positive attitudes than before. Final conclusion of this report is 'close relationship between MTB facility and day life ' and 'tourism's attraction brought them to change their job. This changes gave them satisfaction to their personal life. In socially, it made a large contribution for development of leisure industry by creating new jobs like MTB tour guide and MTB assemblyman.

• Journal of the Korean Chemical Society
• /
• v.33 no.3
• /
• pp.304-308
• /
• 1989

The spectrophotometric determination of $Lu^{3+},\;Eu^{3+}$ and some other rare earths have been investigated using Methyl Thymol Blue(MTB) as spectrophotometric reagent. Rare earth elements form a stable complex with MTB abount pH 6.5 and the ratio of its complex is 1 to 1. MTB has a absorption maxima at 440nm and rare earth MTB complex has absorption maxima 610nm at pH 6.5, respectively. The absorbance of the rare earth MTB complex is stable in 7 hours after color developing and obey the Beer law in the range of $0{\sim}110{\mu}g/50ml$. The ligand such as phosphate, citrate and EDTA decrease the absorbance of its complex considerably, and this method has a poor selectivity of each rare earth element and the molar absorptivity is $1.2{\sim}2.0{\times}10^4mol^{-1}{\cdot}l{\cdot}cm^{-1}$. In methyl alcohol, ethyl alcohol and acetone medium we did not find out any absorption change of the rare earth MTB complex.

• Tuberculosis and Respiratory Diseases
• /
• v.84 no.3
• /
• pp.237-244
• /
• 2021

Background: The emergence of drug-resistant tuberculosis (TB), is a major menace to cast off TB worldwide. Line probe assay (LPA; GenoType MTBDRplus ver. 2) and Xpert MTB/RIF assays are two rapid molecular TB detection/diagnostic tests. To compare the performance of LPA and Xpert MTB/RIF assay for early diagnosis of rifampicin-resistant (RR) TB in acid-fast bacillus (AFB) smear-positive and negative sputum samples. Methods: A total 576 presumptive AFB patients were selected and subjected to AFB microscopy, Xpert MTB/RIF assay and recent version of LPA (GenoType MTBDRplus assay version 2) tests directly on sputum samples. Results were compared with phenotypic culture and drug susceptibility testing (DST). DNA sequencing was performed with rpoB gene for samples with discordant rifampicin susceptibility results. Results: Among culture-positive samples, Xpert MTB/RIF assay detected Mycobacterium tuberculosis (Mtb) in 97.3% (364/374) of AFB smear-positive samples and 76.5% (13/17) among smear-negative samples, and the corresponding values for LPA test (valid results with Mtb control band) were 97.9% (366/374) and 58.8% (10/17), respectively. For detection of RR among Mtb positive molecular results, the sensitivity of Xpert MTB/RIF assay and LPA (after resolving discordant phenotypic DST results with DNA sequencing) were found to be 96% and 99%, respectively. Whereas, specificity of both test for detecting RR were found to be 99%. Conclusion: We conclude that although Xpert MTB/RIF assay is comparatively superior to LPA in detecting Mtb among AFB smear-negative pulmonary TB. However, both tests are equally efficient in early diagnosis of AFB smear-positive presumptive RR-TB patients.

• IMMUNE NETWORK
• /
• v.20 no.5
• /
• pp.37.1-37.15
• /
• 2020

Mycobacterium tuberculosis (Mtb) is an etiologic pathogen of human tuberculosis (TB), a serious infectious disease with high morbidity and mortality. In addition, the threat of drug resistance in anti-TB therapy is of global concern. Despite this, it remains urgent to research for understanding the molecular nature of dynamic interactions between host and pathogens during TB infection. While Mtb evasion from phagolysosomal acidification is a well-known virulence mechanism, the molecular events to promote intracellular parasitism remains elusive. To combat intracellular Mtb infection, several defensive processes, including autophagy and apoptosis, are activated. In addition, Mtb-ingested phagocytes trigger inflammation, and undergo necrotic cell death, potentially harmful responses in case of uncontrolled pathological condition. In this review, we focus on Mtb evasion from phagosomal acidification, and Mtb interaction with host autophagy, apoptosis, and necrosis. Elucidation of the molecular dialogue will shed light on Mtb pathogenesis, host defense, and development of new paradigms of therapeutics.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.6
• /
• pp.989-998
• /
• 2019

Autophagy is crucial for immune defense against Mycobacterium tuberculosis (Mtb) infection. Mtb can evade host immune attack and survival within macrophages by manipulating the autophagic process. MicroRNAs (miRNAs) are small, non-coding RNAs that are involved in regulating vital genes during Mtb infection. The precise role of miRNAs in autophagy with the exits of Mtb remains largely unknown. In this study, we found miR-1958, a new miRNA that could regulate autophagy by interacting with 3'UTR of autophagy-related gene 5 (Atg5). In addition, Mtb infection triggered miR-1958 expression in RAW264.7 cells. What's more, miR-1958 overexpression blocked autophagic flux by impairing the fusion of autophagosomes and lysosomes. Overexpression of miR-1958 reduced Atg5 expression and LC3 puncta while inhibition of miR-1958 brought an increase of Atg5 and LC3 puncta; the opposite results were observed in detection of p62. The survival of Mtb in RAW264.7 cells transfected with mimic of miR-1958 was enhanced. Taken together, our research demonstrated that a novel miR-1958 could inhibit autophagy by interacting with Atg5 and favored intracellular Mtb survival in RAW264.7 cells.

• Journal of Sport and Applied Science
• /
• v.3 no.1
• /
• pp.27-34
• /
• 2019

Purpose: Mountain biking as a sport and recreational activity has grown rapidly over the previous two decades. The purpose of this study is to analyze differences of leisure satisfaction by demographic characteristics of mountain bike participants. Research design, data, and methodology: To accomplish the purpose, the study employed purposive sample method among nonprobability sampling and collected data from 300 participants. Of collected data, 288 responses were analyzed excepted for uncompleted responses. To analyze data, study utilized statistical techniques including descriptive statistics, reliability analysis, independent-sample t-test, and one-way ANOVA, and to verify difference between individual groups in relation to significant results, Duncan's multiple range was conducted. Results: Findings indicated no differences of leisure satisfaction by gender. Similarly, education level and occupations have not influence leisure satisfaction. Yet, differences of leisure satisfaction by participation frequencies were observed. Of participation frequencies, participants riding 5 times a week were more satisfied with MTB participation than the other groups participating less 3 times a week. Conclusions: The findings provide a couple of insights in terms of promoting MTB participation. In specific, results indicated that MTB would be attractive sports that is able to appeal huge populations regardless of their education and occupation. Furthermore, consistent participation is judged an effective way to increase psychological, educational, and social satisfaction. In this sense, regional MTB clubs could play a salient role in motivating riders to more participate in MTB. Hence, establishing strategies for encouraging club activities are needed.

• Korean Journal of Microbiology
• /
• v.54 no.3
• /
• pp.222-227
• /
• 2018

Mycobacterium tuberculosis (MTB)-originated lipid antigen is presented on the antigen-presenting cell surface with CD1b. When monocyte-derived dendritic cells phagocytosed MTB H37Rv (Multiplicity of infection 10, infectivity: 46.89%), the CD1b expression level decreased slowly. Since this was just a live MTB-mediated phenomenon, it was not detected from heat-killed MTB or mycolic acid, which is a unique antigen of MTB. We confirmed that the phosphorylation of CD1b molecules using 2D electrophoresis with staining could phosphorylate and induce the presentation of the lipid antigen using the phagocytosis assay.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.10
• /
• pp.1506-1521
• /
• 2019

Tuberculosis, which is caused by Mycobacterium tuberculosis (Mtb), is among the most pressing worldwide problems. Mtb uniquely interacts with innate immune cells through various pattern recognition receptors. These interactions initiate several inflammatory pathways that play essential roles in controlling Mtb pathogenesis. Although the TLR signaling pathways have essential roles in numerous host's immune defense responses, the role of TLR signaling in the response to Mtb infection is still unclear. This review presents discussions on host-Mtb interactions in terms of Mtb-mediated TLR signaling. In addition, we highlight recent discoveries pertaining to these pathways that may help in new immunotherapeutic opportunities.

• Korean Journal of Food Science and Technology
• /
• v.24 no.4
• /
• pp.361-366
• /
• 1992

Studies were carried out on the factors responsible for the change of pungency in 'Kagdugi'. During fermentation, the pH in 'Kagdugi' decreased to around to 4, while acidity increased continuously. In accordance with the decrease of pungency, the content of 4-methylthio-3-butenyl isothiocyanate (MTB-NCS) in the homogenate of 'Kagdugi' decreased gradually and on 3rd day, the optimum period for good flavor, below 5% of that in the homogenate of fresh radish. The decrease of MTB-NCS formation was accompanied by the gradual loss of 4-methylthio-3-butenyl glucosinolate (MTB-glucosinolate), which was found to disappear more rapidly than total glucosinolate. Also, the ascorbic acid-dependent myrosinase activity was observed to decline gradually with the fermentation time, although the ascorbic acid content varied above the concentration required for maximal enzyme activity. Thus, it was suggested that the decline of MTB-NCS may be related to the gradual losses of MTB-glucosinolate content and myrosinase activity which are both susceptible to the effect of acidic pH.