• 한국환경복원기술학회지
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• 제13권6호
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• pp.97-106
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• 2010

Earthworm, a prominent ecosystem engineer within many terrestrial ecosystems, can exert profound influences on various abiotic/biotic environments through bioturbation processes such as burrowing, casting and mixing of litter and soil. In this study, we investigated how the presence or absence of earthworm (Oligochaeta) can alter the soil physico-chemical conditions and ultimately the distribution and abundance of Collembola which constitutes a large proportion of the soil fauna. During September 2010, soil organisms along with soil samples were collected from randomly installed 20 plots in Mt. Gwan-ak. We examined the differences in the abundance of Collembola among plot samples in respect to the presence/absence of earthworm and soil physico-chemical conditions (i.e., pH, $PO_4^{3-}$, $NO_3^{2-}$, organic matter (OM), electrical conductance and water content). Analysis of soil physico-chemical environment revealed a significantly higher organic matter content and electrical conductance in plots with earthworm compared to plots without earthworm. Abundance of Collembola were not only higher in plots with earthworm than in plots without earthworm, but were also positively correlated with availability of OM present in the environment. The results suggest that positive impacts of earthworm on the abundance of Collembola in this study may have been due to their ability to effectively modify soil physico-chemical conditions favored by Collembola. Such conspicuous influence of earthworm's activity on below-ground community suggests their potential significance in forest restoration or revegetation process.

• 한국수정란이식학회지
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• 제31권1호
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• pp.61-64
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• 2016

We developed a polymerase chain reaction (PCR)-based molecular method for sexing and identification using sexual dimorphism between the Zinc Finger-X and -Y (ZFX-ZFY) gene and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for mitochondrial DNA (mtDNA) cytochrome B (CYTB) gene in meat pieces and commercial sausages from animals of different origins. Sexual dimorphism based on the presence or absence of SINE-like sequence between ZFX and ZFY genes showed distinguishable band patterns between male and female DNA samples and were easily detected by PCR analyses. Male DNA had two PCR products appearing as distinct two bands (ZFX and ZFY), and female DNA had a single band (ZFX). Molecular identification was carried out using PCR-RFLP of CYTB gene, and showed clear species classification results. The results yielded identical information on the sexes and the species of the meat samples collected from providers without any records. The analyses for DNA isolated from commercial sausage showed that pig was the major source but several sausages originated from chicken and Atlantic cod. Applying this PCR-based molecular method was useful and yielded clear sex information and identified the species of various tissue samples originating from livestock.

• 한국수산과학회지
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• 제54권4호
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• pp.467-479
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• 2021

The distribution and abundance of fish eggs and larvae were investigated from February to December 2020 along the coastal waters of Korea. The eggs and larvae were identified using the mitochondrial DNA cytochrome c oxidase subunit I (mtDNA COI) and 16s rRNA gene. During the study period, eggs of overall 45 taxa belonging to 26 families were collected and larvae of overall 39 taxa belonging to 23 families were collected. In Yeongil Bay, eggs of Engraulis japonicus, which accounted for 83.9% of the total population, was the most dominant species, followed by Sardinops sagax (4.0%), Repomucenus valenciennei (3.8%) and E. japonicus larvae, which accounted for 34.9% of the total population. These were followed by Sebastiscus marmoratus (31.0%). In Gomso Bay, E. japonicus eggs accounted for 61.7% of the total population, followed by Sillago japonica (14.0%), Johnius grypotus (8.8%) and Pholis fangi larvae, which accounted for 53.5% of the total population, followed by Ammodytes personatus (34.1%). In Jinhae Bay, E. japonicus eggs accounted for 86.0% of the total population, followed by Leiognathus nuchalis (4.1%), Konosirus punctatus (3.7%) and E. japonicus larvae, which accounted for 48.7% of the total population, followed by Parablennius yatabei (21.6%).

• 한국육종학회지
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• 제43권5호
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• pp.383-390
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• 2011

Near-isogenic lines (NILs) carrying bacterial blight resistance genes (Xa4, xa5 and Xa21) were developed in japonica rice using Suweon345 as genetic background. NILs were selected by gene specific DNA markers and inoculation of K1 or K3a race. NILs conferring Xa4 were resistant to K1, K2, K3, and moderately resistant to K3a. NILs conferring xa5 were resistant to K1, K2, K3, and K3a. NILs having Xa21 were susceptible to K1, while resistant to K2, K3 and K3a. Target genes of NILs with the genetic background of Suweon345 were also confirmed by using eleven Philippines races and International Rice Bacterial Blight (IRBB) NILs carrying Xa4, xa5 and Xa21. All NILs had no significant difference from their recurrent parents in the major agronomic traits except for panicle length and brown rice 1,000 grain weight. Heading date of NILs ranged from Aug. 10 to Aug. 11, which was similar to that of recurrent parent, Suweon345. Culm length, number of grains per panicle and ratio of ripened grain of NILs were similar to those of Suweon345. Milled rice of NILs was ranged from 4.82 to 4.93MT/ha. These NILs will be useful for improving resistance to K3a race of bacterial blight pathogens in Korean japonica cultivars.

• 한국응용곤충학회지
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• 제59권4호
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• pp.409-410
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• 2020

본 보고는 2020년 산림 지역에서 대발생하여 큰 피해를 준 매미나방과 대벌레에 의한 피해면적과 피해수종에 대해 기술하였다. 매미나방에 의한 식엽 피해는 강원(1,638 ha), 경기(1,134 ha), 충북(726 ha), 서울(476 ha) 등 중부지방을 중심으로 심각하였다. 대벌레는 서울시 은평구와 고양시 덕양구 사이에 위치한 봉산(약 19 ha)에서 대발생하였다.

• Fisheries and Aquatic Sciences
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• 제25권12호
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• pp.601-618
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• 2022

The genus Semisulcospira is an economically and ecologically valuable freshwater resource. Among the species, Semisulcospira coreana, Semisulcospira forticosta and Semisulcospira tegulata are endemic to the Korean peninsula and Semisulcospira gottschei is widespread in Asia. Therefore, maintenance and conservation of wild populations of these snails are important. We investigated the genetic diversity and population structure of Semisulcospira based on the mitochondrial cytochrome c oxidase subunit I (COI), NADH dehydrogenase subunit 4 (ND4), and combined mitochondrial DNA (COI + ND4) sequences. All four species and various genetic makers showed a high level of haplotype diversity and a low level of nucleotide diversity. In addition, Fu's Fs and Tajima's D neutrality tests were performed to assess the variation in size among populations. Neutrality tests of the four species yielded negative Fu's Fs and Tajima's D values, except for populations with one haplotype. The minimum spanning network indicated a common haplotype for populations of S. coreana, S. tegulata and S. gottschei, whereas S. forticosta had a rare haplotype. Also, genetic differences and gene flows between populations were assessed by analysis of molecular variance and using the pairwise fixation index. Our findings provided insight into the degree of preservation of the species' genetic diversity and could be utilized to enhance the management of endemic species.

• 한국육종학회지
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• 제43권6호
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• pp.549-553
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• 2011

'중모1012호'는 국립식량과학원 상주출장소에서 2010년도에 육성한 조생 고품질 고식미 내수발아성 중간모본으로 주요 특성과 수량성을 요약하면 다음과 같다. 1. 남부중산간지, 북부평야지 및 중산간지, 남부고냉지 및 동북부해안지 보통기 보비재배에서 평균 출수기가 7월 31일로 '오대벼'보다 2일 늦은 조생종이다. 2. 간장은 78 cm로 '오대벼'보다 크며, 수당립수는 많고 등 숙비율이 높은 중립종에 속한다. 3. 위조현상은 나타나지 않았으며 성숙기 하엽노화가 늦고 수발아는 '오대벼'보다 안되는 내수발아성이다. 내냉성은 보통이다. 4. '중모1012호'는 도열병에 저항성 반응을 보였다. 5. 입형은 현미장폭비가 1.85로 단원형이며 심복백이 약간있으나 맑고 투명하다. '오대벼'에 비해 아밀로스함량은 낮고 단백질함량은 약간 높으며 밥맛은 매우 양호하다. 6. 쌀수량은 보통기 표준재배에서 5.55 MT/ha로 '오대벼'보다 3% 많은 편이다.

• 한국환경복원기술학회지
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• 제6권6호
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• pp.72-85
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• 2003

The purpose of this study is to establish the basis of the ecological restoration on the limestone mines in Korea, through the research of the revegetation measures of the disturbed slopes in Japanese limestone mines and the analysis of the case of Ibuki and Bukou limestone mines to realize the restoration works successfully. According to the number of mines to carry out the revegetation works increase every year, the revegetation area and amount of planting also go on increasing in Japan. In the small mines the environmental problem is not recognized seriously, but some mines have muchly invested in the revegetation of the damaged land by mines. Ibuki mine in Shiga-gen is practicing the four principles for the restoring of the revegetation in the disturbed slopes by mines. Firstly, the mean slope preserves $37^{\circ}$ in the last rock cut slopes. Secondly, the last rock cut slopes to rehabilitate are covered with the soil dressing 30cm high. Thirdly, the grasses in the mining area are digged up beforehand, and then they are reused to the revegetation works of rock cut slopes. Lastly, from the upper rock cut slopes the revegetation works begin, the process of the above are repeated, as the result, the restoring works will be completed. Upon the ground of these principles, the complex of the original vegetation and the native species seeding, that is, the revegetation technique of Ibuki have established. In the difficult area to revegetate, sodding works, heaping up vegetation-sack, seed-spraying, heaping up soil-sacks and planting works are adapted variously. Bukou mine in Saitama-gen try to maintain the revegetation, the screening and the stability of the enormous mining rock cut slope, and for these works, the activity of 'Chichibu Working Group of Rock Wall for Limestone Mines' contributes largely. The chief technique of the revegetation in Bukou mine is following; after the making the berm on the rock cut slopes, the soil dressing is filled in it, and the trees is planted for the screening of the rock cut slopes. They devide the rock cut slopes into four typical types, and choose the different ways to make the berm and fill the soil dressing in characteristic. Most of the planting trees are consist ofthe native species of Mt. Bukou through the stages of the nursery and transplantation. Not only the revegetation of the demaged land but also the breeding of the conservational plants also are their important business.

• 한국전통조경학회지
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• 제29권4호
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• pp.14-26
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• 2011

임대정원림은 전라남도 화순군 남면 사평리 상사마을 끝자락에 서북향하여 자리하는데, 16C 남언기에 의해 조성된 고반원을 기반으로 1862년 사애 민주현에 의해 작정되었으며, 봉정산과 사평천을 배산임수하여 풍수적으로 번영과 행복을 상징하는 학체형국(鶴體形局)의 서출동류하는 길지이다. 공간구성은 '사애선생장구지소'로 각자된 표석과 향나무가 어우러져 있는 앞뜰(전원(前園))과 정자와 방지원도형 연못을 포함하는 '대상부(臺上部)'의 내원(內園), 신선도를 둔 2개의 연못으로 이루어진 '대하부(臺下部)'의 수경원(水景園) 그리고 봉정산 수림과 외남천(사평천) 및 농경지를 포함하는 외원(外園) 등 총 5개 권역으로 구성됨으로써 임대정을 중심으로 위요 중첩된 원림적 속성이 강하게 표출된다. 시문과 현장조사 결과, 임대정원림은 안분지족의 의미를 담고 있는 기임 등 임수대산(臨水對山)한 터에 수심양성의 거점을 경영코자 했던 은일처로서의 장구지소, 귀거래를 위한 별서원림, 대자연의 도와 일체화를 이루고자 한 세심처(洗心處) 등의 작정동기가 확인되며, 애련설에 근거한 향원익청(香源益淸)적 개념의 피향지와 읍청당, 방지원도 그리고 도합 3개로 이루어진 삼신선도의 신선사상 등 다양한 의경 요소가 함축된 원지(園池)를 보이고 있다. 임대정원림의 경관처리기법으로는 봉정산의 원경과 달빛 투영미를 의도한 인경(引景), 사방에 펼쳐진 산수경관과 문화경관의 환경(環景), 대경(對景)으로서 도입된 목가적 전원의 차경, 수륜대로의 앙경(仰景)과 연못으로의 부경(俯景), 정자와 오솔길에서 취할 수 있는 정관(靜觀)과 동관(動觀), 계류수와 연못 등 수경공간의 인경(隣景), 버섯 또는 우산처럼 보이도록 유도된 임대정으로의 관경(觀景), 버드나무 어우러진 연못의 간경(看景), 격물치지의 의미를 담아 관념을 대입한 암각글씨의 의경(意境), 해돋이와 해넘이를 동시에 취할 수 있는 취경(取景) 등이 중첩되어 펼쳐지는 다양한 경관기법이 발견된다. 건립 초기에는 주로 은행나무, 대나무, 버드나무, 소나무, 잣나무, 전나무, 뽕나무, 가래나무, 오동나무 그리고 매화와 연 등의 식물이 도입된 것으로 보이는데, 전반적으로 유가적 품격을 반영하거나 도가적 풍류관, 미래번영을 염원하는 봉황사상 등이 내재된 의미경관적 면모를 엿볼 수 있다. 더불어 연못 주변에 도입된 열식법, 정자 주변에 펼쳐진 총식과 환식법, 녹음수를 활용한 점식법, 기념식수한 오동나무 등의 고식법, 소나무와 전나무를 양과 음으로 대응시킨 대식법 등 다양한 식재기법을 추적할 수 있다.

• 한국환경농학회지
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• 제32권4호
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• pp.332-337
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• 2013

신간척지에서 청보리의 안정재배를 위한 토양 염농도를 제시하기 위하여 새로 조성된 새만금간척지에서 토양염농도(EC) 0.8, 3.1, 6.5, 11.0 dS/m에 해당하는 지점을 선정하고, 화학비료로 $N-P_2O_5-K_2O$=150-100-100 kg/ha를 시용한 후 2011년 10월 26일에 영양보리를 220kg/ha 파종하였다. 재배기간중 토양 염농도는 수확기>출수기>파종기>월동기 순으로 파종기에 비해 수확기에 1.4~4.2배 상승하였다. 염농도가 높을수록 발근에 지장을 초래하여 지상부 생육도 저조하였고 특히 EC 11.0 ds/m에서 출현은 하였으나 겨울철이 지난 후 고사하였다. 수량은 토양염농도 0.8 dS/m(8.8 MT/ha)에 비해 3.1 dS/m과 6.5 dS/m에서 각각 68 %, 35 % 수준이었다. Proline함량은 생육초기가 수확기보다, 그리고 염농도가 높을수록 많았다. 수확기의 $Na_2O$와 MgO 함량은 염농도가 높을수록 많았고 N, $P_2O_5$, $K_2O$ 및 CaO 함량은 염농도가 높을수록 적었다. 따라서 토양 염농도가 3.1 dS/m이상으로 높아짐에 따라 청보리의 지하부와 지상부에 심각한 생육저해로 양분의 불균형 흡수를 초래하여 수량이 저조하기 때문에 안정한 청보리 생산을 위해서는 지하 배수시설 또는 관개수를 이용하여 3.1 dS/m이하로 염농도를 떨어뜨린 후 재배하는 것이 바람직할 것으로 판단된다.