• Journal of dental hygiene science
• /
• v.18 no.4
• /
• pp.265-270
• /
• 2018

The purpose of this study was to investigate the chemical properties of some commercially available mouthwashes and to ascertain whether the mouthwashes accelerated mineral loss in dental enamel. Five commercially available mouthwashes were selected from the three largest malls in Korea: Perio Total 7 Aqua Cool Mint Strong $Fresh^{TM}$ (PS; LG Household & Health Care Ltd.), Garglin $Original^{TM}$ (Dong-A Pharmaceutical Co., Ltd.), Garglin $Zero^{TM}$ (Dong-A Pharmaceutical Co., Ltd.), Listerine Naturals $Citrus^{TM}$ (LC; IDS Manufacturing Ltd.), and Listerine Cool $Mint^{TM}$ (LM; IDS Manufacturing Ltd.). The composition, pH, and titratable acidity of the mouthwashes were investigated. Six bovine teeth specimens were prepared for each mouthwash group. Each of the six specimens was individually immersed in 30 ml aliquots of mouthwash for 1 minute, 30 minutes, 90 minutes, and 120 minutes, and the samples were placed in a $36.5^{\circ}C$ stirred incubator. The degree of mineral loss (${\Delta}F$) of the tooth surface area exposed to mouthwash, compared with normal teeth, was analyzed by quantitative light-induced fluorescence-digital. The difference in ${\Delta}F$ among mouthwash groups was examined by the Kruskal-Wallis H test (${\alpha}=0.05$). The contents of mouthwashes differed between Listerine and other products, and the pH ranged from 4.09 to 6.75. The titratable acidity of PS was the lowest at 0.63 ml and highest at 9.25 ml for LM. Minor mineral loss was observed when dental specimens were immersed in the Listerine products (LC and LM) for more than 90 minutes, but the degree of mineral loss for Listerine products was not statistically significantly different from that for groups without mineral loss. In conclusion, all five commercially available mouthwashes showed no harmful effects on tooth enamel.

• Analytical Science and Technology
• /
• v.36 no.2
• /
• pp.89-98
• /
• 2023

Analytical methods for detecting atranol, chloroatranol, evernic acid, (+)-usnic acid, and atranorin in sanitary napkins and mouthwashes were developed using ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). UHPLC-MS/MS conditions were optimized for rapid, sensitive, and simultaneous analysis of the five allergenic compounds. The methods were validated by assessing their specificity, matrix effects, limit of detection (LOD), limit of quantification (LOQ), linearity, accuracy, and precision. Good linearity was achieved with a determination coefficient of ≥0.99. The LOD and LOQ were 2.1-9.8 and 6.4-29.6 ng/g for sanitary napkins and 0.29-0.48 and 0.87-1.45 ng/mL for mouthwashes, respectively. The accuracy and precision were within an acceptable range according to the criteria reported in the European SANTE/11813/2017 guidelines (70-120 % recovery, <20 % relative standard deviation). Therefore, these methods can be used to analyze atranol, chloroatranol, evernic acid, (+)-usnic acid, and atranorin in sanitary napkins and mouthwashes.

• Journal of Digital Convergence
• /
• v.10 no.6
• /
• pp.331-339
• /
• 2012

The easy and simple simultaneous analytical method of preservatives (BA, SA, DHA, MP, EP, IPP, PP, IBP and BP) was studied by more easily changing from method used in food and drug using HPLC with scherzo SM-C18 column. All presevatives were seperated successfully in mobile phase of 50 mM ammonium formate : 0.1% phosphoric acid (50:50 v/v%) and 50 mM ammonium formate : acetonitrile (30 : 70). Retention time of BA, SA, DHA, MP, EP, IPP, PP, IBP and BP was 7.74, 9.08, 12.57, 13.83, 21.62, 27.29, 28.20, 33.20 and 33.68 min, respectively. The calibration curves of BA, SA, DHA, MP, EP, IPP, PP, IBP and BP were linear over the concentration range of 5~80 ${\mu}g/mL$ with correlation coefficient of above 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) of BA, SA, DHA, MP, EP, IPP, PP, IBP and BP were 0.52 and 1.58, 1.09 and 3.29, 1.00 and 3.03, 1.36 and 4.13, 1.26 and 3.83, 1.02 and 3.08, 1.11 and 3.37, 0.82 and 2.48, 0.85 and 2.59 ${\mu}g/mL$, respectively. The coefficients of variation for intra- and inter-day assay were 0.12~2.68 and 0.18~2.66%, respectively. The developed method showed good intra- and inter-day precision and accuracy. The preservatives used in mouthwashes were BA, MP and PP and were detected in 24 samples(86%) except for 4 samples and not showed significant difference in using dose of adult and children. In conclusion, the developed method can be useful for simultaneous analysis of preservatives in mouthwashes and these results suggest that could be applied to fundamental study and guideline on content of preservatives in mouthwashes.

• BMB Reports
• /
• v.48 no.1
• /
• pp.42-47
• /
• 2015

Streptococcus mutans is frequently associated with dental caries. Bacterial fermentation of food debris generates an acidic environment on the tooth surface, ultimately resulting in tooth deterioration. Therefore, various mouthwashes have been used to reduce and prevent Streptococcus mutans. The aim of this study was to evaluate the antimicrobial activities of 4 commercial mouthwashes and those of 10% and 20% ethanol solutions (formula A, B, C, D, E and F) against Streptococcus mutans using biofilm and planktonic methods. The range of reduction in the viable cell count of Streptococcus mutans as estimated by the biofilm and planktonic methods was 0.05-5.51 log ($P{\leq}0.01$) and 1.23-7.51 log ($P{\leq}0.001$) compared with the negative control, respectively, indicating that the planktonic method had a stronger antibacterial effect against S. mutans. Among the tested formulations, formula A (Garglin $regular^{(R)}$ mouthwash) was the most effective against Streptococcus mutans ($P{\leq}0.001$).

• Journal of Korean society of Dental Hygiene
• /
• v.23 no.4
• /
• pp.227-234
• /
• 2023

Objectives: Cetylpyridinium chloride CPC-based mouthwashes are well known to have no harmful ingredients in the mouth and can be used for a long time. The purpose of this study was to evaluate the effect of using CPC-based mouthwashes to suppress the biofilm formation and antibiotics for handling orthodontic appliances. Methods: To measure the antibacterial effect, Streptococcus mutans (S. mutans) cultured orthodontic appliances were precipitated in Gargreen and Polident for 5 minutes, incubated at 37℃ for 24 hours(h). In order to measure the biofilm removal effect, the degree of biofilm formation on the orthodontic appliances was stained with a methylene blue and the difference before and after was compared using image J software program (NIH Image J; NIH, Bethesda, MD). Results: The viability of S. mutans according to the concentration showed that Gargreen and Polident inhibited colony formation compared to the control, respectively (p<0.01). The degree of biofilm formation was significantly higher in the control, however both Gargreen and Polident significantly reduced it compared to the before and after condition on removable orthodontic appliances (p<0.01). Conclusions: This study suggests that the use of Gargreen, a cetylpyridinium chloride based oral cleaning cleanser, could be replaced by Polident for antibacterial effect and biofilm formation on removable orthodontic appliances.

• Journal of Periodontal and Implant Science
• /
• v.9 no.1
• /
• pp.30.1-30.1
• /
• 1979

• Journal of Periodontal and Implant Science
• /
• v.15 no.1
• /
• pp.86.2-86.2
• /
• 1985

• Journal of dental hygiene science
• /
• v.13 no.4
• /
• pp.434-439
• /
• 2013

This study was conducted to compare the dental plaque reduction using various mouthwashes with Quantitative Light induced Fluorescence-Digital (QLF-D). A survey on 20 students was carried out. The students who were at Shingu College. Experimental group was gargled 20 ml of Listerine during 30 seconds and 15 ml of Hexamedine during 60 seconds. Control group was gargled distilled water during 30 second. The data were analyzed with t-test using SPSS 20.0 program. The ratios of control group and experimental group were reduced. Degree of ${\Delta}R30$ and ${\Delta}R70$ Listerine group was a significant difference (p>0.05). Degree of Simple Plaque Score and ${\Delta}R30$ Hexamedine group was a significant difference (p>0.05). There was no significant difference in the distilled water gargle group (p<0.05). The result of this study has the effect of two mouthwashes reduced dental plaque. The evaluation data of this study will be used in clinical application and research about QLF-D.

• International Journal of Oral Biology
• /
• v.44 no.1
• /
• pp.27-29
• /
• 2019

Halitosis is a very common disease that affects the majority of the population and is characterized by unpleasant odor during expiration. Anaerobic bacteria produce a range of malodorous substances including volatile sulfur compounds. To reduce oral malodor, the amount of oral microorganisms should be managed through brushing, scraping, and use of antibacterial agents. In this study, a mouthwash containing 0.05% cetylpyridinium chloride was tested on 22 candidates with oral malodor for two weeks to confirm oral malodor reduction through the use of antibacterial mouthwashes. Volatile sulfur compound measurements were significantly lower after using the mouthwash than before using it; thus, the mouthwash effectively reduced oral malodor.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48
• /
• pp.41-48
• /
• 2003

The number of natural products obtained from plants has now reached over 100,000 and new chemical compounds are being discovered ever year. Medicinal and Aromatic plants and their extracts have been used for centuries to relieve pain, aid healing, kill bacteria and insects are important as the antifungal and anti-herbivore agents with further compounds being involved in the symbiotic associations. Although their functions in plants have not been fully established, it is Known that some substances have growth regulatory properties while others are involved in pollination and seed dispersal. The complex nature of these chemicals are usually produced in various types of secretory structures which is an important character of a plant family and also influenced and controlled by genetic and ecological factors. Detailed anatomical description of these structures ave relevant to the market value of the plants, the verification of authenticity of a given species and for the detection of substitution or adulteration. Volatile oils are used for their therapeutic action for flavoring of lemon, in perfumery of rose or as starting materials for the synthesis of other compounds of turpentine. For therapeutic purposes they are administered as inhalations of eucalyptus oil, peppermint oil, as gargles and mouthwashes of thymol and transdermally many essential oils including those of lavender, etc. With these current trend for using volatile components in essential oil will be increasing in the future in Korea and in the world as well.