• Korean Journal of Medicinal Crop Science
• /
• v.12 no.3
• /
• pp.255-261
• /
• 2004

The MeOH extracts from 21 species were tested for their cytotoxicity against SK-MEL-28 melanoma cell line and HaCat normal cell line in 5 g/ml by sulforrhodamine-B (SRB) method. Among them, the MeOH extract from Moutan Cortex Radicis showed the moderate activity with the growth rate of 74.3% in SK-MEL-28 cells and the high activity with the growth rate of 207.8% in HaCat cells. Activity-guided fractionation was performed and five compounds, paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy- acetophenone (4), paeonfliorin (5) were isolated from hexane and EtOAc fraction. The structures were established by physicochemical and spectrometric methods $(mp,\;UV,\;IR,\;^1H-NMR,\;^{13}C-NMR,\;spectram)$. All compounds were evaluated for their cytotoxicity, compound 4 showed the significant cytotoxic activity with $ED_50$ value of $5.92\;{\mu}g/ml$, but the other compounds no activity. These results suggest that compound 4 is a novel anticancer candidate against SK-MEL-28 melanoma cells.

• Korean Journal of Medicinal Crop Science
• /
• v.6 no.2
• /
• pp.96-101
• /
• 1998

This experiment was carried out to investigate the effect of fertilizers on yield and paeonol content of Paeonia moutan Sims according to cultivation years. Tested variety was a local cultivar and fertilizer treatments used were compost (3, 000kg/10a), N-P-K (10-10-10kg/10a) fertilizer, fowl dropping manure (100kg/10a), organic fertilizer (200kg/10a) and non-fertilizer. Root yield increased by 12% in N-P-K fertilizer, 18% in compost, 22% in fowl dropping manure and 26% in organic fertilizer treatment compared with non-fertilizer. Increment rate of root yield according to cultivation years from 3 to 6 year cultivation was highest in 5 year cultivation. Paeonol content was highest of 3.08% at October harvest and 3.63% in the fowl dropping manure treatment. Paeonol content was higher at longer cultivation year, but increment rate of paeonol content per year was low from 3 year cultivation. Partial paeonol content of root increased in the order of lower part (3.07%), middle part (2.77%), and upper part (2.38%).

• Korean Journal of Food Science and Technology
• /
• v.36 no.3
• /
• pp.472-477
• /
• 2004

Hypoglycemie effect of Moutan Radicis Cortex (MRC) extract contained in Yukmijihuang-hwan was determined by investigating insulin-sensitizing and ${\alpha}-glucoamylase-suppressing$ actions. MRC was extracted with 70% ethanol, fractionated by XAD-4 column chromatography with mixture solvent of methanol and water, and utilized for hypoglycemic effect assay. Significant insulin sensitizing activities of MRC extracts were observed in 3T3-L1 adipocytes, giving MRC extracts with 1 ng/mL insulin reach glucose uptake level increased by 50 ng/mL of insulin alone. MRC methanol extracts of 20, 40, 60, and 80% suppressed ${\alpha}-glucoamylase$ activity in vitro. Peak serum glucose levels and area under curve were lower in Sprague Dawley male rats treated with MRC ethanol extract than those treated with cellulose in oral glucose tolerance test using 2 g dextrin/kg body weight. These data suggest MRC extracts contain effective insulin -sensitizing and ${\alpha}-glucoamylase-suppressing$ compounds for hypoglycemic activity.

• Journal of Korean Medicine for Obesity Research
• /
• v.20 no.2
• /
• pp.69-77
• /
• 2020

Objectives: In this study, we investigated the antiobesity and antidiabetic effects of polyherbal extract, DM2 consisting of Atractylodis Rhizoma, Anemarrhenae Rhizoma, Cinnamomi Cortex, and Moutan Radicles Cortex in high fat diet-induced obesity mice. Methods: DM2 extract was prepared with a hot water. Six-week-old male C57BL/6N mice were fed a high-fat diet (HFD) for 8 weeks and then administrated with DM2 extract (500 mg/kg, p.o.) for 4 weeks. The changes of physiological markers, body weight (BW), food and water intakes, and the levels of fasting blood glucose (FBG) were measured once a week for 4 weeks in mice. The the serum levels of glucose, insulin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (T-CHO), triglyceride, and low density lipoprotein cholesterol in sera were measured in mice using autometic chemical analyzer and enzyme linked immunosorbant assay. We also observed the histological changes of liver and pancreatic tissues with Hematoxylin & Eosin staining. Results: In physiological change, the increases of BW, calorie intake, and FBG in HFD-induced obese mice were significantly decreased after administration of DM2 extract for 4 weeks. The decrease of water intake was significantly increased in DM2 extract-administrated mice. In serological change, the administration of DM2 extract in obesity mice was significantly decreased the serum levels of glucose, insulin, T-CHO, AST, and ALT levels. We also found that DM2 extract inhibited the increase of lipid droplets in liver and the structural destruction of pancreatic tissues in obesity mice. Conclusion: Our study demonstrated that DM2 extract has antiobesity antidiabetic effects with body weight loss, decrease of glucose and insulin levels, and lipid accumulation on liver tissue.

• 한국약용작물학회:학술대회논문집
• /
• 2010.10a
• /
• pp.363-364
• /
• 2010

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.18 no.4
• /
• pp.1071-1077
• /
• 2004

Mudanpi (Cortex Moutan Radicis; the root cortex of Paeonia suffruticosa Andrews) is an important Chinese crude drug used in many oriental prescriptions. 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose (PGG), a major component of this crude drug, has been shown to possess potent antioxidant, anti-mutagenic and anti-proliferative effects. In this study, I examined whether PGG could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 expression and HO activity. Exposure of Neuro 2A cells to PGG (10-50μM) resulted in a concentration- and time-dependent induction of HO-1 mRNA, and protein expressions and heme oxygenase activity. PGG protected the cells from hydrogen peroxide-induced cell death. The protective effect of PGG on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. These results indicate that PGG is a potent inducer of HO-1 and HO-1 induction is responsible for the PGG-mediated cytoprotection against oxidative damage.

• Journal of Korean Medical classics
• /
• v.23 no.6
• /
• pp.1-14
• /
• 2010

The conclusions after studying the rules and the principles of the six-drugs mixing forming the prescription of Yukmijihwanghuan(六味地黃丸) are as follows: 1. Yukmijihwanghuan cares for the three viscera; the liver, the spleen and the kidney and the three entrails; the urinary bladder, the gall bladder, and the stomach and it strengthens them as well. The drug can be used mainly for the cure of the Three Yang Channels of Foot and the Three Yin Channels of Foot. 2. The three drugs of Yukmijihwanghuan; Rehmanniae Radix, Dioscoreae Rhizoma, Corni Fructus altogether has the tonifying effect and the other three; Alismatis Rhizoma, Moutan Cortex, Poria has the purging effect. The first three kinds of drugs tonifies and the last three kinds of drugs purges. While these two groups of drugs are pitted against each other, they also balance each other harmoniously increasing the curative effect(remedial[curative] value). 3. Yukmijihwanghuan cools off the lung which is under metal category, helps the spleen, an earth category to be strong. It also adds the Water Qi to the kidney so that it stabilizes the Fire Qi. 4. Yukmijihwanghuan helps the kidney strongly, helps the urine to be excreted well, cools down the Fire Qi and makes dry things wet. 5. Rehmanniae Radix, the principal drug of Yukmijihwanghuan and Corni Fructus, the minister drug of the medicine have a taste of thick and are materially heavy. The two drugs do the descending action that it tonifies Yin Qi and adds Essence of Life. The other ingredients of Yukmijihwanghuan; Moutan Cortex, Poria, Alismatis Rhizoma have effects on lowering the Fire Qi. If Fire Qi descends, then Water Qi ascends. Yukmijihwanghuan has an efficacy of lowering Fire Qi and increasing Water Qi.

• Journal of Haehwa Medicine
• /
• v.8 no.2
• /
• pp.191-210
• /
• 2000

The Literatual Study on Treatment of Toothbleeding Through the literatual study on treatment of toothbleeding, we concluded as follows, 1. The causes of the toothbleeding are divided into heat of stomach (胃熱) and deficiency of renal (腎虛). If caused by heat of stomach, the treatment methods are Ryang-Hyul-Ji-Hyul(凉血止血), Cheong-Yul-Sa-Hwa(淸熱瀉火). If caused by deficiency of renal, the treatment methods are Ryang-Hyul-Ji-Hyul(凉血止血), Ja-Eum-Gang-Hwa(滋陰降火). 2. The medications are several - taking a medicine for internal use(內服法), keeping with one's mouth full of an infusion and spoutting it Haamsoobup(含漱法), and others like taking a medicine for external use(外用法) only. 3. The treatment drugs divided by medication are as follows : Taking a medicine for internal use : Cheongwesan(淸胃散), Gamroeum(甘露飮), Joweseunggitang(調胃承氣湯), Seogagjihwangtang(犀角地黃湯), Yugmijihwangtang(六味地黃湯), Palrnihwan(八味丸), Soansinhwan(小安腎丸) etc... Haamsoobup : Gudogeum(救毒飮), Jihwangtang(地黃湯), Mangchosan(莽章散) etc... Taking a medicine for external use : Pilseungsan(必勝散), Rogposan(綠包散, Hyunggeousan(荊槐散), Bingoksan(氷玉散), Sahyangsan(麝香散), Ulgeumsan(鬱金散), Yongnoisan(龍腦激) are used. 4. A coposition of the medication : Cheong-Yul medicine(淸熱藥), Bo-IK medicine(補益藥), Ji-Hyul medicine(止血藥), Gae-Gyu medicine(開竅藥), Su-sap medicine(收澁藥) are mainly used. Cheong-Yul medicine : Rehmanniae Radix(鮮地黃), Moutan Cotex(牧丹皮), Coptidis Rhizoma(黃連), Scutellariae Radix(黃芩) Bo-Ik medicine : Glycyrrhizae Radix(甘草), Angelicae gigantis Radix(當歸), Rehmanniae Radix Preparat(熟地黃), Ginseng Radix(人蔘) Ji-Hyul medicine : Biotae Cacumen(側柏葉), Sophorae Flos(槐花), Typhae Pollen(蒲黃) Gae-Gyu medicine : Moschus(麝香), Bomeolum(氷片) Su-sap medicine : Alumem(白礬) 5. A result of the observation on the medication : Taking a medicine for internal use : Glycyrrhizae Radix, Rehmanniae Radix Preparat, Rehmanniae Radix, Moutan Cortex, Scutellariae Radix are used. Yugmijihwangtang and Cheong-Yul medicine are mainly used. Taking a medicine for external use : Alumem, Moschus, Salt(鹽), Asari herba cum Radice(細辛) are mainly used. Especially there is a method that uses a burnt drug. Haamsoobup and taking a medicine for external use : Medicines of Cheong-Yul-Ryang-Hyul(淸熱凉血) and Su-Ryum-Ji-Hyul(收斂止血) are used.

• Biomolecules & Therapeutics
• /
• v.24 no.5
• /
• pp.536-542
• /
• 2016

Shuangdan oral liquid (SDO) containing radix Salviae miltiorrhizae (Chinese name Danshen) and cortex moutan (Chinese name Mudanpi) is a traditional Chinese medicine using for treating vascular diseases. Danshensu (DSS) is a main effective monomer composition derived from radix Salviae miltiorrhizae and paeonol (Pae) from cortex moutan. Although the two herbs are widely used in traditional Chinese medicine, the pharmacological functions of their active compositions were not reported. Therefore, the research of DSS and Pae in mechanisms and pharmacodynamics interaction can provide scientific evidence to support clinical application. The diabetic nephropathy (DN) rats which were induced by streptozotocin (STZ) were treated with SDO, DSS, Pae, and DSS+Pae for eight weeks. The positive effects on DN animal models were investigated by detection of physiological and biochemical indexes and oxidative stress markers, within five treatments: SDO, DSS, Pae, DSS+Pae and insulin group. Compared with the model group, the DSS+Pae group improved the renal function, blood lipid metabolism and blood viscosity, increased the vitality of T-SOD or T-AOC and decreased the level of MDA or NO after the treatment. The study was successfully showed that the DSS+Pae group could delay the process of DN, especially in the renal injury part of histopathology changes. Our results suggest that the co-administration of DSS and Pae significantly may play a protective role in DN rats through decreasing the oxidative stress and improving the blood lipid metabolism mechanisms.

• Journal of Food Hygiene and Safety
• /
• v.22 no.2
• /
• pp.93-98
• /
• 2007

Acute toxicity of WK-38, a herbal preparation for the atherosclerosis, was examined using male and female Sprague-Dawley rats. WK-38 is composed of Rhei Rhizoma, Magonoliae Cortx, Moutan Cortex Radicis. Rats were treated with the WK-38 intragastrically at 0 mg/kg, 5 mg/kg, 50 mg/kg, 500 mg/kg or 2,000 mg/kg and observed for two weeks. No mortality was observed at the doses used. Abnormal clinical signs such as eye bleeding, nasal bleeding and hyperemia had been shown temporary after administration. All rats were appeared to be healthy and normal during the 2 week observation. Also there was no difference in net body weight gain, gross pathological findings, and urine analysis among the groups rats treated with different doses of the WK-38.