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Lee, Su-Heon;Kim, Sang-Mok;Kim, Woo-Chang;Lee, Key-Woon
- Proceedings of the Korean Society of Plant Pathology Conference
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- 2003.10a
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- pp.150.1-150
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- 2003
A single-step multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of five cucurbit-infecting viruses： cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV), and kyuri green mottle mosaic virus (KGMMV). The multiplex RT-PCR provides a simple and rapid method for detecting various viruses in cucurbit plants, which will help diagnose many cucurbit plants at a time.
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이학래;신동소;전준경
- Journal of Korea Technical Association of The Pulp and Paper Industry
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- v.29 no.3
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- pp.60-68
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- 1997
To develop paper coating technology for preventing print mottling, which is recognized as the most common printing problems in using coated papers, ink absorption properties of various coated papers were examined by employing K 8T N and Croda ink absorption tests. The influences of pigment compositions, coat weights and drying methods on these ink absorption properties were examined, and then the variations of ink absorption ratio were analyzed and discussed. It is demonstrated that the variation of Croda ink absorption ratio induced by coat weight variation can be utilized most succesfully to predict print mottling propensity of coating formulations. A systematic analysis approach was suggested that can be employed in the development of the most desirable coating color formulation to achieve target optical properties with the least print mottling.
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Park, Chung Youl;Min, Hyun-Geun;Lee, Hong-Kyu;Maharjan, Rameswor;Yoon, Youngnam;Lee, Su-Heon
- Research in Plant Disease
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- v.24 no.4
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- pp.348-352
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- 2018
A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed for the detection of Clover yellow vein virus (ClYVV), Peanut mottle virus (PeMoV), and Tomato spotted wilt virus (TSWV), which were recently reported to infect soybean and azuki bean in Korea. Species-specific primer sets were designed for the detection of each virus, and their specificity and sensitivity were tested using mixed primer sets. From among the designed primer sets, two combinations were selected and further evaluated to estimate the detection limits of uniplex, duplex, and multiplex RT-PCR. The multiplex RT-PCR assay could be a useful tool for the field survey of plant viruses and the rapid detection of ClYVV, PeMoV, and TSWV in leguminous plants.
https://doi.org/10.5423/RPD.2018.24.4.348 인용 PDF KSCI HTML

Park, J.H.;J.H. Sung;H.Y. Shin;M.U. Chang;S.N. Yoo
- Proceedings of the Korean Society of Plant Pathology Conference
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- 2003.10a
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- pp.150.2-150
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- 2003
Carnation mottle carmovirus(CarMV) was isolated from Lilium spp. in Korea. This isolate, CarMV, was done bioassay, which plants were Dianthus caryophyllus, Gomphrena globosa, Chenopodium amaranticolor, Dianths chinensis. CarMV was propagated on the leaves of Chenopodium amaranticolor with the crude-sap inoculation method and purified by Mossops method(1976). We produced antiserum against CarMV and analyzed the antiserum specificity with ELISA, Gel diffusion method, and Rapid Immunofilter Paper Assay (RIPA). From these results of the assay, RIPA method was simple and rapid for CarMV detection. We have established successfully the CarMV detection system. CarMV coat protein gene was amplified by RT-PCR with specific primers and sequencing analysis was done.
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Park, M.H.;B.E. Min;K.H. Ryu
- Proceedings of the Korean Society of Plant Pathology Conference
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- 2003.10a
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- pp.146.1-146
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- 2003
Transgenic Nicotiana benthmiana plants harboring and expressing coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for both virus-resistant screening and complementation analysis of related viruses and environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N. benthamiana was performed using Agrobacterium-mediated method and the pZGCPPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and -susceptible lines, were obtained by screening of challenging homologous virus for T1 generations. Complementation of CP-deficient related virus was analyzed using the susceptible line of ZGMMV. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.
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