• 대한한의학방제학회지
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• 제24권4호
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• pp.323-333
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• 2016

Objectives : The purpose of this study was to investigate musk deer in taxonomic status and genus species. We investigated the characteristic of musk deer for medicinal usages. Methods : To identifications of musk deer on the taxonomic status and genus species, the literary investigation were conducted on the Korean, China, and Japan pharmacopoeia and published herbal books, CITES Species Lists too. Results : Musk deer placement in a separate family status is the Moschidae. M. chrysogaster Hodgson and M. sifanicus Przewalski was the same species. So, Alpine musk deer revised M. chrysogaster Hodgson[=M. sifanicus Przewalski]. Geographic distribution of M. moschiferus L. divided Sibirica group and himalaica group. Group himalaica contains three subspecies: Korean musk deer(M. moschiferus parvipes Hol.), Chinese musk deer(M. chrysogaster Hodgson), and Himalayan musk deer(M. leucogaster Hodgson). The genetic divergence between M. moschiferus L. and other species was clearly distinguished from the others. M. berezovskii Flerove was less than the others. However, the divergence among M. chrysogaster Hodgson, M. fuscus Li, and M. leucogaster Hodgson were quite low. Musk deers are mostly distributed around the high-plateau. Moschus were from Nepal, Bhutan, Russia, China. Forest musk deer(M. berezovskii Flerove) farming was conducted in China from 1950s. In the Korean hebal pharmacopoeia, Moschus include l-muscone($C_{16}H_{30}O$ : 238.40) over 2% for quantitative test. Conclusions : There are three species of musk deer, Siberian musk deer(M. moschiferus L.), forest musk deer(M. berezovskii Flerove), and Alpine musk deer(M. chrysogaster Hodgson) for medicinal usages.

• 한국환경복원기술학회지
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• 제11권4호
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• pp.91-102
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• 2008

This study was conducted to obtain the basic information about Siberia Musk Deer (Moschus moschiferus), and to establish a conservation area for the replacement of Siberia Musk Deer into Mt. Odea National Park, South Korea. We analyzed the main habitat for Siberia Musk Deer restoration in Mt. Odae National Park, Korea. We recorded Siberia Musk Deers' field signs (i.e., bedding sites, feeding areas, feces and tracks), and habitat variables such as nearest distance to the watercourse, trails, slope, aspect, forest type and land cover etc. from Dec. 2004 to Aug. 2007 in Mt. Jiri, Mt. Seorak, and Demilitarized Zone (DMZ). Rocky areas, ridges, mixed coniferous forest (Red pine and Mongolian oak), southern and southeastern slope, elevation of above 800m, and distance from watercourse shorter than 300m etc. were dominant habitat types of Siberia Musk Deer. Based on the significant habitat types, a proper conservation plan should be prepared for the restoration of habitats in order to reintroduce, and to increase the population of Siberia Musk Deer in Mt. Odea National Park, South Korea. Small population size, and limited ecological data are the major constraints for the establishment of conservation areas. Furthermore, other anthropogenic factors including human activities, poaching, and residential area around the park caused the difficulties to qualify as a conservation area. For the successful establishment of conservation area in the future, it is recommended to conduct a comprehensive ecological research, and to survey human disturbances including their impacts on the habitats of Siberia Musk Deer in the Mt. Odea National Park.

• 한국동물위생학회지
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• 제30권3호
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• pp.459-466
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• 2007

The Korean Musk deer (Moschus Moschiferus) is endangered due to heavy hunting and Massive destructions of natural habitat. They are included in CITES appendix II. In addition they are designated as a precious natural product in 1968 and listed as endangered species by the Government and Cultural Heritage Administration. At present, the number of musk deer in Korea is smaller than minimum viable population. Without increment of the population size, natural restoration is impossible. It is necessary to develop the artificial growth and re-enforcement methods in order to conservation Korean musk deer. Furthermore, It is necessary to ensure that we have an adequate individual group for artificial growth and re-enforcement. To ensure that we have an adequate individual group, it is necessary to know ecology of musk deer. This study is conducted to confirm and investigate of habitat of musk deer in order to capture individual musk deer. we investigated, confirmed habitats, found traces and captured using trap and decoy. Captured musk deer is male and 5.5 kg, this have canine tooth of 1.5cm, is estimated 15 months old. After capture, we measured each parts of body and tested to research of parasite infection. Strongyloides papillous was founded in the feces. It is essential to get healthy individual and establish of artificial growth technique.

• 혜화의학회지
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• 제9권2호
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• pp.315-324
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• 2001

To investigate the capacity of anti-inflammatory cytokines and biological response modifiers (BRMs) to induce IL-$1{\beta}$, IL-6, TNF-${\alpha}$ gene overexpression from mouse macrophages, we isolated the resident peritoneal macrophages from BALB/c mouse (8 week old) and incubated for 6 h with lipopolysaccaride (LPS) and Moschus moschiferus (MOMS) extracts. Analysis of inflammatory cytokines gene expression was carried out by RT-PCR amplification. Amplified PCR products were electrophoresed on 1.2% agarose gel, and the analysis (Ht) was used to 1D-density program. 1. LPS and MOMS extract treatments resulted in a significant decrease in IL-$1{\beta}$, IL-6, TNF-${\alpha}$ mRNA expression level compared with the LPS treatment. 2. Among four sample of MOMS, Inhibitory effects of MOMS-A and MOMS-D for inflammatory cytokines gene expression were to be fine compared with the MOMS-Band MOMS-C. According to the above data, Because the anti- tumoral and anti-inflammatory response activities of macrophage are known to be dependent on the production of inflammatory cytokines (IL-$1{\beta}$, IL-6, TNF-${\alpha}$) by macrophages, we suggest that evaluations of BRM for the reduction of inflammatory cytokines production by macrophages are important for clinical application.

• 대한한의학회지
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• 제16권2호
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• pp.100-114
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• 1995

This bibliographic study was designed to research indications of the Moschus which was representatively used for resuscitation in acute stage of stroke throughout successive books of oriental medicine. Especially, in this study, severe cases with disturbance of consciousness and acute stage of stroke were investigated. The results were summarized as the followings: 1. The Moschus was dried material which secreted from aromatic vescicle of Moschus moschiferus L.. which was pungent in flavor, warm in nature, nontoxic and strongly aromatic. The Moschus removed the obstruction in the channels and used to early stage of stroke. 2. The Moschus was aromatic resuscitatable medicine, which recovered disturbance of consciousness due to stroke. 3. The Moschus had excitatory actions of respirtory center and vascular motor center, heart stimulating action, inhibitatory action of the platelet aggregation and anti thrombin action and recovered cerebral ischemic change. According to these actions, the Moschus could be used cerebral infarction. 4. Prescriptions included the Moschus was used to symptoms of unconsciousness, secretion, stuporous mental state, dysarthria, facial palsy and hemiplegia. That was Angungwoohwangh wan(安宮牛黃丸), Woohwangchungshimwon(牛黃淸心元), Sahyangsohaphangwon(麝香蘇合香元), etc. According to the above mentioned results, the Moschus was aromatic resuscitatable medicine which was used for symptoms of conscius disturbance, convulsion, sudden fainting etc., promoted cerebral blood flow and had good effect on acute stage of stroke and severe cases with conscious disturbance. Therefore, it is considered that the Moschus must be studied for effect of cerebral infarction and hemorrhage in clinic with animal's and pharmacological experimental results.

• 환경생물
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• 제25권3호
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• pp.215-222
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• 2007

비무장지대(DMZ)를 포함한 민통선 일대는 지난 50여 년간 사람에 의한 인위적인 간섭을 적게 받은 지역으로 자연생태계가 잘 보전된 세계적인 환경보전지역이다. 그러나 최근 들어 개발에 따른 급격한 환경파괴가 가속화되고 있어 이에 대한 대비가 시급한 실정이다. 현재까지 발표된 조사지역에 대한 논문, 보고서 등 문헌자료와 청문조사를 토대로 조사된 포유동물상을 파악하였다. DMZ 및 군사접경지역 전체에서 서식하는 포유동물 중 문헌상으로 서식이 확인된 종은 모두 6목 17과 51종으로 조사되었으며, 이중 화천군, 철원군, 양구군 일대 중부지역에는 총 6목 14과 32종이 서식하는 것으로 조사되었다. 천연기념물로 지정된 종은 반달가슴곰(Ursus thibetanus), 수달(Lutra lutra), 사향노루(Moschus moschiferus), 산양(Nemorhaedus caudatus), 하늘다람쥐(Pteromys volans), 물범(Phoca largha)등 3목 6과 6종이었다. 이 조사를 통해 얻어진 종합적인 자연문화재 기초자원 조사결과는 향후, UNESCO의 세계자연보전지역 등록에 대비한 기초자료로 활용될 수 있을 것으로 판단된다.

• 대한본초학회지
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• 제36권4호
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• pp.41-50
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• 2021

Objectives : This study is to check the quality of Moschus products in Korean markets using a chromatographic analysis. Methods : We collected musk products, two genuine products from Russia (A, B), two authentic products from Hong Kong (C) and Korea (D), the Ministry of Food and Drug Safety (MFDS) standard (E), and two fire ants products (E, F). Results : For identification, TLC analysis of 60% ethanol extracts of each product showed that A, B, C, and E have the suitable patterns before color development at 365 nm UV light for the MFDS regulation in Korea. A clear red spot was observed from the E at R_f 0.6 with 365 nm UV light after color development, but this spot was not found in A and B. For the purity test, a distinct violet spot with R_f 0.87 was observed from the A and B in TLC analysis of methanol extracts of each product at white light, however, this spot was not matched with the impurities of E and F. In HPLC-UV pattern analysis, a similar peak pattern was shown in A, B and E, and similar peaks were observed C, although the similarity was weaker than that of A, B, and E. The F and G showed different peak patterns compared with the peak patterns of other samples. Conclusions : Hence, it is considered that the test methods need flexibility in application for identification and purity test depending on the type of sample.

• 감성과학
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• 제8권2호
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• pp.155-160
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• 2005

본 연구는 관절통 모델에서 웅담 우황과 웅담 우황 사향의 약침액의 효과를 검사하기 위해 수행되었다. 할로탄 마취하에서 관절통은 수컷 쥐의 관절강내에 $2\%$ carrageenan을 주입하여 유발시켰다. 운동자극에 대한 감각신경의 반응은 약침을 시술하기 전과 후에 기록하였다. 경혈에 주입한 약침은 유해한 운동 자극에 대한 신경의 반응을 억제시켰다 족삼리에 시술한 약침은 합곡에 비해 유해한 자극에 대한 관절 감각신경의 반응을 더 많이 억제시켰다. 이러한 결과는 웅담 우황과 웅담 우황 사향의약침이 관절통을 완화하는 데 효과적인 치료법을 제공할 수 있다는 것을 시사한다.

• Journal of Acupuncture Research
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• 제26권1호
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• pp.121-133
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• 2009

Objectives : The purpose of this study is to find out the clinical possibility of Bufonis Venenum used without toxicity and side effect. Methods : We investigated the pharmacological effects, toxicity and processing of Bufonis Venenum through the literatures and studies. Results : Bufonis Venenum is made by parotid gland of dermato gland of Bufo bufo gargarizns or B. melanostictus Schneider, and it is dried for using. The medical ation of Bufonis Venenum are cardiotonic, respiration stimulation, anticancer, topical anesthesia. The toxic symptoms of Bufonis Venenum are relative with digestive, circulatory, nervous system similared with digitalis toxicity. It is important to take 0.015-0.03g by mouth, external use about 1-4% 0.5-3ml and 2-8ml injections by 20ml mix to 5% dextrose fluid. Bufonis Venenum is processed to prevent toxicity and evaluate efficacy by alcohol and milk. There are 68 prescription consisted by Bufonis Venenum in KTKP(Korean Traditional Knowledge Portal). They usually use for antiabcess, anticancer with Moschus moschiferus(麝香), Cinnabar(朱砂). Conclusions : The results from above literary studies show that internal, external medicine and Aqua-acupuncture of Bufonis Venenum could be clinically used to sedative, antiinflammatory, anticancer and topical ataralgesia without toxity through optimum dose and processing.

• Molecules and Cells
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• 제26권3호
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• pp.314-318
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• 2008

Korean long-tailed goral (Nemorhaedus caudatus) is one of the most endangered species in South Korea. However, detailed species distribution and sex ratio data on the elusive goral are still lacking due to difficulty of identification of the species and sex in the field. The primary aim of this study was to develop an economical PCR-RFLP method to identify species using invasive or non-invasive samples from five Korean ungulates: goral (N. caudatus), roe deer (Capreolus pygargus), feral goat (Capra hircus), water deer (Hydropotes inermis) and musk deer (Moschus moschiferus). The secondary aim was to find more efficient molecular sexing techniques that may be applied to invasive or non-invasive samples of ungulate species. We successfully utilized PCR-RFLP of partial mitochondrial cytochrome b gene (376 bp) for species identification, and sex-specific amplification of ZFX/Y and AMELX/Y genes for sexing. Three species (goral, goat and water deer) showed distinctive band patterns by using three restriction enzymes (Xbal, Stul or Sspl). Three different sexing primer sets (LGL331/335 for ZFX/Y gene; SE47/48 or SE47/53 for AMELX/Y gene) produced sex-specific band patterns in goral, goat and roe deer. Our results suggest that the molecular analyses of non-invasive samples might provide us with potential tools for the further genetic and ecological study of Korean goral and related species.