• 한국동물학회지
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• 제22권4호
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• pp.153-164
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• 1979

A total of 352 specimens of congeneric species of Agkistrodon was collected and morphometric analysis and starch-gel electrophoresis were carried out in order to investigate the taxonomic status of this genus. The results obtained in this study are as follows: Morphometric analysis 1. Three species are recognized based on Gloyd's criteria. There was no specimen that was doubtful to classify correctly. Therefore, it seems that Gloyd's morphological characters are good criteria to identify each species. 2. All three species are sympatric at two localities (Mt. Seolag, Gangwon-do, and Mt. Deogyu, Jeonra-bugdo) and A. caligino년 and A. b. brevicaudus are sympatric at Gwangneung and Mt. Yongmun, Gyonggi-do. No hybrids were found in these sympatric localities. 3. Notable sexual dimorphism was found in meristic characters. 4. A. saxatilis has signicantly more number of ventrals and shorter tail ratio than other two species. 5. There were no significant meristic character differences between A. caligino년 and A. b. brevicaudus. Genetic analysis 1. Among 26 loci investigated, 12 loci (46.1%) were identical in their mobility, 4 loci (15.4%) were nearly identical with minor frequency differences, and 10 loci (38.5%) showed interspecific mobility difference. 2. The average proportion of polymorphic loci was 9.03%. This is considerably less than that of other vertebrates. 3. The average S and D values between species are .695 and .342 respectively. These values indicate that three taxa are distinct species. 4. A. caliginosus is genetically more related to A. saxatilis than to Agkistrodon, namely A. b. brevicaudus, A. caligino년 and A. saxatilis, as proposed by Gloyd. Far from being mere morphological variants, as suggested by Kang and Yoon (1975), they are highly distinctive evolutionary units.

• Journal of Nutrition and Health
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• 제32권8호
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• pp.864-869
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• 1999

To investigate the effect of dietary phosphatidylcholine(PPC) supplement on memory improvement, biochemical study on the brain, and morphometric studies on the cholinergic neurons in the rat basal forebrain were undertaken. The pregnancy rats were divided into the normal control, the choline deficient and the PPC supplemental groups according to quantity of the PPC in diet. According to choline deficiency and PPC supplement after birth, the neonate rate of the normal control group were subdivided into the control diet(N-N) and the PPC supplied (N-S) groups, the choline deficient group were subdivided into the continually deficient (D-D), the control diet(D-N) and the PPC supplied groups(D-S), and the PPC supplemental group were subdivided into the control diet (S-N)and the continually supplied (S-S)group. The PPC supplemented diet was added 2% egg PPC in AIN 76 formula diet. PPC concentrations and cholinesterase(CE) activities were measured in the serum, the liver and the brain, respectively. Immunohistochemical stains for choline acetyltransferase(ChAT) was employed for the morphological and morphometric studies. The maze test was undertaken to evaluate memory improvement. PPC concentration and CE activities in the serum, liver and the brain were high in the PPC supplemental groups and low in the choline deficient groups. ChAT immunoreactivity neurons at the medial septal diagonal bond complex and the basal forebrain nucleus of Meynert were reduced in the choline deficient groups. Average failure rate for the maze test was the lowest in the S-S group and the highest in the D-D group. Insufficient choline suppley during the neuronal development would result in cholinergic neuronal damage, which could be prevented by adequate PPC supplement. It is consequently suggested that PPC supplement may be effective on memory improvement by maintaining the cholinergic neuronal activity in the basal forebrain of the rats.

• Journal of Korean Neurosurgical Society
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• 제41권6호
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• pp.377-381
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• 2007

Objective : The aim of this study was to evaluate the morphometric changes in neuroforamen in grade I isthmic spondylolisthesis by anterior lumbar interbody fusion [ALIF]. Methods : Fourteen patients with grade I isthmic spondylolisthesis who underwent single level ALIF with percutaneous pedicle screw fixation were enrolled. All patients underwent standing lateral radiography and magnetic resonance imaging [MRI] before surgery and at 1 week after surgery. For quantitative analysis, the foraminal height, width, epidural foraminal height, epidural foraminal width, and epidural foraminal area were evaluated at the mid-portion of 28 foramens using T2-weighted sagittal MRI. For qualitative analysis, degree of neural compression in mid-portion of 28 foramens was classified into 4 grades using T2-weighted sagittal MRI. Clinical outcomes were assessed using Visual Analogue Sale [VAS] scores for leg pain and Oswestry disability index before surgery and at 1 year after surgery. Results : The affected levels were L4-5 in 10 cases and L5-S1 in 4. The mean foraminal height was increased [p<0.001], and the mean foraminal width was decreased [p=0.014] significantly after surgery. The mean epidural foraminal height [p<0.001], epidural foraminal width [p<0.001], and epidural foraminal area [p<0.001] showed a significant increase after surgery. The mean grade for neural compression was decreased significantly after surgery [p<0.001]. VAS scores for leg pain [p=0.001] and Oswestry disability index [p=0.001] was decreased significantly at one year after surgery. Conclusion : Foraminal stenosis in grade I isthmic spondylolisthesis may effectively decompressed by ALIF with percutaneous pedicle screw fixation.

• Fisheries and Aquatic Sciences
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• 제25권7호
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• pp.380-389
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• 2022

Morphometric relationships, condition factor (CF) and reproductive biology are significant tools in fish stock management, fish biology, physiology, conservation and ecology. Growth and reproductive strategy of Oreochromis niloticus were studied from 343 fish specimens collected from Lake Shala between January and December 2018. Fish samples ranged from 7.7 cm to 33.0 cm in total length (TL) and from 7.80 g to 708.21 g in total weight (TW) were collected using gillnets of 4, 6, 8, 10, and 12 mm mesh sizes. The length-weight relationship of O. niloticus was TW = 0.0104TL^3.19, indicating positive allometric growth of the fish. The sex ratio (0.93:1) was insignificant from the ideal fish distribution of 1:1 (χ² = 0.47, p > 0.05). Mean CF for males, females and combined sexes was 1.04, 1.06 and 1.05, respectively and statistically insignificant (p > 0.05). The spawning peak occurred in July (rainy) and February (dry) periods, as defined by ripe females and the breeding season. Absolute mean fecundity was 806 eggs and correlated positively with TL and TW of the fish (p < 0.05) (F = 0.56TL^2.29, R² = 0.93, p < 0.05; F = 18.83TW^0.67, R² = 0.90, p < 0.05). The study provides the first detailed account of the morphometric relationships and reproductive biology of O. niloticus in Lake Shala, which can be used as baseline information for successive biological-based studies in Soda Lakes of Ethiopia.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.569-581
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• 2023

The morphology and genetic identification of Rasbora lateristriata and Rasbora argyrotaenia between cultivated and wild populations has never been reported. This study compares morphology and cytochrome c oxidase (COI) genes between farmed and wild stock Rasbora spp. in Java and Sumatra island, Indonesia. We analyzed the truss network measurement (TNM) characters of 80 fish using discriminant function analysis statistical tests. DNA was extracted from muscle tissue of 24 fish specimens, which was then followed by polymerase chain reaction, sequencing, phylogenetic analysis, fixation index analysis, and statistical analysis of haplotype networks. Basic Local Alignment Search Tool analysis validated the following species: R. lateristriata and R. argyrotaenia from farming (Jogjakarta); Rasbora agryotaenia (Purworejo), R. lateristriata (Purworejo and Malang), Rasbora dusonensis (Palembang), and Rasbora einthovenii (Riau) from natural resources. Based on TNM characters, Rasbora spp. were divided into four groups, referring to four distinct characters in the middle of the body. The phylogenetic tree is divided into five clades. The genetic distance between R. argyrotaenia (Jogjakarta) and R. lateristriata (Malang) populations (0.66) was significantly different (p < 0.05). R. lateristriata (Purworejo) has the highest nucleotide diversity (0.43). R. argyrotaenia from Jogjakarta and Purworejo shared the same haplotype. The pattern of gene flow among them results from the two populations' close geographic proximity and environmental effects. R. argyrotaenia had low genetic diversity, therefore, increasing heterozygosity in cultivated populations is necessary to avoid inbreeding. Otherwise, R. lateristriata (Purworejo) had a greater gene variety that could be used to develop breeding. In conclusion, the middle body parts are a distinguishing morphometric character of Rasbora spp., and the COI gene is more heterozygous in the wild population than in farmed fish, therefore, enrichment of genetic variation is required for sustainable Rasbora fish farming.

• Anatomy and Cell Biology
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• 제56권1호
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• pp.32-38
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• 2023

The Galenic venous system plays a vital role in the drainage of blood from deeper parts of the brain. This venous system is contributed by many major veins. These veins are located closer to the pineal gland making the surgical approach in this region difficult. Any accidental injury or occlusion of the vein of Galen could lead to devasting results. Thus, studying the dimensions of the vein of Galen is more important. Hence, we aimed to evaluate the morphometry and trajectory to the vein of Galen. About 100 computed tomographic venography records were evaluated and the length, diameter of vein of Galen, angle between straight sinus and vein of Galen and distance from internal occipital protuberance and roof of fourth ventricle to vein of Galen were studied. The mean length and diameter of vein of Galen were 9.8±2.7 and 4.08±1.04 respectively. The mean angle between straight sinus and vein of Galen was 64.2°. The mean distance between external occipital protuberance and roof of fourth ventricle to vein of Galen were 52±6.9 and 33.3±4.5 respectively. No significant morphometric differences were observed between the age groups as well as between the sexs. The results obtained from this study may be helpful for the neurosurgeons in better understanding of the anatomy of the Galenic venous system and to adopt a safe surgical approach to improve the efficacy of the surgeries of the pineal gland and also in the region of vein of Galen.

• 치과방사선
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• 제29권1호
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• pp.87-103
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• 1999

Purpose: This study was undertaken to quantitatively estimate the degree of the damage and recovery of the irradiated rat condylar cartilage using the Image Analyzer. Materials and Methods: Experimental animals were 16 male rats of the Sprague-Dawley strain at the age of 20 day irradiated with the dose of 10 Gy in their head and neck region. Four rats were sacrificed at the each of the following time intervals - 1, 4, 7 and 14 days, respectively. The same number of control group animals were sacrificed at the each age of 21. 24, 27 and 34 days, respectively. The specimens were stained with 0.5％ toluidine blue and examined with light microscope. The condylar cartilage was divided into 4 zones; fibrous zone, proliferating zone, upper hypertrophic zone, and lower hypertrophic zone. And then, the proliferating zone was subdivided into 2 layers - upper and lower layer, and upper and lower hypertrophic zone were subdivided into three layers, respectively - upper, middle and lower layer. With the aid of Image Analyzer, morphometric analysis was performed. The thickness, the numerical density of cells, the cell area density, the extracellular matrix area density, the mean area of single cell, the mean area of extracellular matrix per single cell were measured and analysed. Results: In the experimental group, the thickness of the fibrous zone was slightly increased and that of the proliferating zone and the upper and the lower hypertrophic zone was markedly decreased. With time, the thickness of the fibrous zone was gradually increased and that of the proliferating zone and the upper and the lower hypertrophic zone was steadily in the decreased state. The numerical density of cells of the proliferating zone was increased on post-irradiated 1 day, but decreased after post-irradiated 4 day, and that of the upper hypertrophic zone was decreased. The numerical density of cells of the lower hypertrophic zone was decreased in the early stage and then was decreased or not significantly different from that of the control group with time. In the experimental group, the cell area density of the fibrous zone and the proliferating zone was decreased in the early stage and then gradually increased or not significantly different from that of the control group with time. The cell area density of the upper and the lower hypertrophic zone was varied with time. The extracellular matrix area density value were totally opposite to the cell area density values: The mean area of single cell of the fibrous zone and the proliferating zone was .decreased on post-irradiated 1 day, and increased after post-irradiated 4 day. The mean area of single cell of the upper hypertrophic zone was varied with each layer and time. In the experimental group, the mean area of extracellular matrix per single cell of the fibrous zone was not significantly different with control group, and that of the proliferating zone was decreased on post-irradiated 1 day, and increased after post-irradiated 4 day. The mean area of extracellular matrix per single cell of the lower hypertrophic zone was increased in the early stage. and that of upper hypertrophic zone was varied with each layer and time. Conclusion: The condylar cartilages of rats were affected by irradiation, but the changes were vaned with each layer and time. By morphometric analysis. the changes of the cells of the condylar cartilage of irradiated rat could be calculated quantitatively.

• 대한임상검사과학회지
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• 제38권3호
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• pp.203-207
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• 2006

The purpose of this study was to develop discriminant analysis models for predicting cervical normal/dysplasia case diagnoses using cytometric features derived from the digital image analysis of cell monolayers. The database consisted of 19 cases diagnosed either as normal (n=5), moderate dysplasia (n=7), severe dysplasia (n=7) on monolayer preparations. We studied the nuclear and cytoplasmic characteristics of cells in the normal, moderate dysplasia and severe dysplasia on cervical samples. The morphometric parameters selected for the analysis were nuclear/cytoplasmic ratio and the nuclear variations measured by image analysis on normal and precancerous lesions of cervical smears; several shape factors; area; perimeter; maximal, minimal and equivalent circle diameters. The results showed that the dysplasia samples exhibited changes in both cellular and nuclear form and size but lacked substantial differences in the tumor grades. The coefficient of nuclear variation is as follows to normal cell $21.8{\pm}3.2%$, moderate dysplasia $33.5{\pm}6.1%$, severe dysplasia $27.7{\pm}5.8$ of cervical smears.

• ETRI Journal
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• 제42권1호
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• pp.101-107
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• 2020

For Korean language processing, morphological analysis is a critical component that requires extensive work. This morphological analysis can be conducted in an end-to-end manner without requiring a complicated feature design using a sequence-to-sequence model. However, the sequence-to-sequence model has a time complexity of O(n²) for an input length n when using the attention mechanism technique for high performance. In this study, we propose a linear-time Korean morphological analysis model using a local monotonic attention mechanism relying on monotonic alignment, which is a characteristic of Korean morphological analysis. The proposed model indicates an extreme improvement in a single threaded environment and a high morphometric F1-measure even for a hard attention model with the elimination of the attention mechanism formula.

• Animal Systematics, Evolution and Diversity
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• 제33권4호
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• pp.256-261
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• 2017

Eucephalobus oxyuroides (de Man, 1876) Steiner, 1936 belonging to the family Cephalobidae Filpijev, 1934 (Cephalobomorpha) is newly reported from South Korea. This species is distinguished from other Eucephalobus species by its pointed tail terminus, high lips and relatively longer female tail and body. From many previous studies, intraspecific variation in this species has been reported for body length, absence/presence of probolae, and number/position of papillae in the posterior region of male, posing an obstacle for identification and phylogenetic analysis. In this study, details of the morphological characters and morphometric of E. oxyuroides Korean population are described and illustrated based on optical microscopy.