• 한국발생생물학회지:발생과생식
• /
• 제23권4호
• /
• pp.345-353
• /
• 2019

Higher thermal exposure can influence the blood cell morphology and count. Hence, based on the previous results (Rahman et al., 2019), the present study evaluated the morphometric indices of erythrocytes and their nucleus under different water temperatures (25℃, 28℃, 31℃, and 34℃) to investigate their use as an indicator of thermal stress in red spotted grouper, Epinephelus akaara. 180 healthy specimens of E. akaara were exposed to four temperature conditions (25℃ as control, 28℃, 31℃, and 34℃) for 42 days, following 2 weeks of acclimation at 25℃. Erythrocyte major axis (EL), erythrocyte minor axis (EW), nucleus major axis (NL), and nucleus minor axis (NW) were examined from the blood smears on each sampling day (i.e., 2, 7, and 42 days of thermal exposure). EL and NL were significantly decreased, whereas EW and NW were increased at higher water temperature (31℃ and 34℃). The major-minor axis proportions of erythrocytes and their nucleus (EL/EW; NL/NW) were decreased with increasing water temperature (31℃ and 34℃). The strong relationships were observed among the morphometric indices of erythrocytes and their nucleus, especially in EL vs. NL and EW vs. NW. This study reveals that elevated water temperature (31℃ and 34℃) can influence the major and minor axis morphometry of erythrocytes and their nucleus in red spotted grouper. These indices may be used as stress indicators to monitor the health status of E. akaara and probably for other fish species.

• Ecology and Resilient Infrastructure
• /
• 제5권4호
• /
• pp.264-275
• /
• 2018

The experiment was conducted to investigate the effects of various dietary conditions on external morphometric traits, and sectioned morphometric traits in the cultured cyprinid loach, (Misgurnus anguillicaudatus) for 28 days. For the fed group there was an increase in body weight, standard length, and morphometric dimensions of the head and body cavity regions compared with the starved and initial groups. Sectioned morphometric trait analysis revealed that relative to the starved group, the fed group had greater body circumference, cross-sectional area, and total height (P<0.05). Our results provide data on external and sectioned morphometric changes under starvation conditions, and can be used as a guide to assist in the regulation and scheduling of feeding, and as indices of the nutritional status of cyprinid loach.

• Ocean and Polar Research
• /
• 제34권2호
• /
• pp.165-173
• /
• 2012

A 26 day experiment was conducted to determine the effects of feeding and starvation on the survival, morphology, and histology in chum salmon (Oncorhynchus keta) fry. We included three experimental groups: starved, fed, and initial. The survival and growth rates were lower in the starved group than in the fed group (P < 0.05). In the starved group, survival began to decline after 16 days, and all fish had died after 26 days. We determined the effects of starvation on the morphometric parameters using the truss and classical dimensions. The dimensions in the head region were larger in the starved group than in the initial and fed groups. In contrast, the truss dimensions of the fed group were larger than those of the initial and starved groups. Starvation reduced the heights of the hepatocyte nuclei and of the intestinal epithelium (P < 0.05). The starved group also showed atrophy of the digestive structures and shrinkage of the foregut and midgut. Starvation led to the degeneration and atrophy of the exocrine pancreas, in which the lumen was markedly diminished and the folds of mucosa were less apparent. The hepatocyte morphology in the starved group was abnormal compared with that of the initial and fed groups, with highly compact, irregularly shrunken nuclei. Melanomacrophages were randomly distributed in the kidneys of the starved group, and their abundance increased rapidly during the experiment. In contrast, neither the initial nor fed group had any melanomacrophages. These results suggest that the nutritional parameters used in this study are useful indices of nutritional status in chum salmon.

• 한국발생생물학회지:발생과생식
• /
• 제18권1호
• /
• pp.33-41
• /
• 2014

We investigated the process of yolk absorption in Korean rose bitterling, Rhodeus uyekii, and determined the changes in its morphometric characteristics. The R. uyekii from 1 days post hatching (DPH) to 21 DPH, the eye diameter (ED) was 5.4 at 5 DPH. Thereafter, the ED/total length (TL) ratio increased to 10.7 at 21 DPH (p<0.05). The yolk length (YL) decreased from 95.4 to 1.1 by 21 DPH, and this rate of decrease was greater than that for any other dimension (p<0.05). 12 morphometric dimensions/TL for the R. uyekii were measured at each sampling day from 21 DPH to 170 DPH. At just hatching, the average TL and BW were $6.1{\pm}0.09mm$ and $4.9{\pm}0.07mg$, respectively. At 53 DPH, the average TL was $12.9{\pm}0.28mm$ and the average BW was $14.7{\pm}0.72mg$; the total length growth equation was $TL=5.507e^{0.038t}$ ($R^2=0.916$). Further, the body weight growth equation was $BW=3.3647e^{0.0296t}$ ($R^2=0.9354$). The dimensions with regard to body depth showed the greatest growth rates in the external characteristics of the fish (p<0.05). The patterns of the morphometric characteristics measured in this study can be classified in three ways. The patterns were shown to be increased (y=0.0992x+12.07, $R^2=0.8333$), decreased (y=-0.0569x+42.029, $R^2=0.8395$) or maintained (y=0.005x+18.263, $R^2=0.3678$) from 21 DPH to 170. These results will provide useful indices for the successful rearing of the R. uyekii.

• 한국발생생물학회지:발생과생식
• /
• 제21권1호
• /
• pp.55-62
• /
• 2017

We used flowcytometry to ploidy verification after that investigate difference between diploid and spontaneous triploid through the truss dimension and classical dimension at crucian carp, Carassius auratus, crucian carp C. cuvieri and common carp, Cyprinus carpio collected from Hangang river, Hantangang river, Imjingang river, Geumgang river, Yeongsangang river and Nakdonggang river, Korea. There were significant differences among the three species for the truss dimensions anterior origin of dorsal fin $(2){\times}$ anterior origin of anal fin (5), $2{\times}$ anterior origin of pelvic fin (6), $2{\times}$ origin of pectoral fin (7), posterior origin of dorsal fin $(3){\times}5$, $3{\times}6$, and $3{\times}7$ (P<0.05). There were no significant differences among the three species in the truss dimensions dorsal fin length ($2{\times}9$) and eye diameter (ED)(P>0.05). On the other hand, there were no significant differences in the several classical dimensions of each species (P>0.05). Three classical dimensions, most anterior extension of the head $(1){\times}2$, $1{\times}6$ and $2{\times}$ most posterior scale in lateral line (4) did not differ between the C. auratus diploid and spontaneous triploid (P>0.05). Two classical dimensions, $1{\times}6$ and longest length between most anterior extension of the head and gill cover ($1{\times}8$) did not differ between the C. cuvieri diploid and spontaneous triploid (P>0.05). One classical dimensions, $1{\times}2$ did not differ between the common carp diploid and spontaneous triploid (P>0.05). There were significant differences in the each diploid and triploid species (P<0.05). These results suggest that the classification of each species and classification between diploid and spontaneous triploid morphometrical parameters used in this study are useful indices of morphometrical status in the each species from major river of Korea.

• 대한소아치과학회지
• /
• 제31권2호
• /
• pp.228-235
• /
• 2004

하악 과두의 발달과 증식은 측두하악관절 부위의 생역학적 환경의 변화에 따라 변경될 수 있다. 이 부위에 전달되는 생역학적 하중은 섭취하는 음식물의 경도를 다르게 함으로써 변화시킬 수 있다. 이번 연구의 목적은 성장 중인 쥐에서 부드러운 음식물의 섭취에 의해 저작력을 변화시키는 것이 하악 과두의 해면골의 형태를 변화시킬 수 있는지 미세전산화 단층촬영을 이용하여 분석하는 것이었다. 생후 21일 된 C57BL/6 쥐 36마리를 무작위로 두 그룹으로 나누었다. 8주 동안 대조군의 쥐들은 일반적인 딱딱한 덩어리의 사료를, 실험군의 쥐들은 덩어리의 사료를 잘게 갈은 후 물과 섞어 부드럽게 만들어 먹였다. 또한 실험군의 쥐들의 하악 절치를 일주일에 두 번씩 잘라서 짧게 만들었다. 8주 후 모든 쥐들을 희생하여 우측 하악 과두를 준비하였다. 미세전산화 단층촬영과 삼차원 영상 분석프로그램을 이용하여 하악 과두 해면골의 bone volume(BV), bone surface(BS), total volume(TV) bone volume fraction(BV/TV), surface to volume ratio(BS/BV), trabecular thickness(Tb. Th.), structure model index(SMI)와 degree of anisotropy(DA)를 측정하고 이들 값으로부터 trabecular number(Tb. N.)와 trabecular separation (Tb. Sp.)을 계산하였다 미세전산화 단층영상을 얻은 후 하악 과두의 조직 표본을 만들었다. 연구 결과는 다음과 같았다. 1. Bone volume fraction(BV/TV), trabecular thickness(Tb. Th.)와 trabecular number(Tb. N.)가 대조군에 비해 부드러운 음식을 먹인 실험군에서 유의하게 감소되었다(p<0.05). 2. Trabecular separation(Tb. Sp.)은 부드러운 음식을 먹인 실험군에서 유의하게 증가하였다(p<0.05). 3. Surface to volume ratio(BS/BV), structure model index(SMI), degree of anisotropy (DA)는 두 군 사이에 유의한 차이를 보이지 않았다(p>0.05). 4. 조직 절편을 관찰한 결과, 부드러운 사료를 먹인 실험군에서 하악 과두의 연골층의 증식 층과 전체 두께가 상당히 감소하였다.