• Title/Summary/Keyword: Morphological measurements

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Isolation and Characterization of Bacillus subtilis CH-10 Secreting Cellulase from Cattle Manure (우분으로부터 Bacillus subtilis CH-10의 분리 및 균주가 분비하는 Cellulase의 특성에 관한 연구)

  • Kim, Tae-Il;Han, Jung-Dae;Jeon, Byoung-Soo;Ha, Sang-Woo;Yang, Chang-Bum;Kim, Min-Kyun
    • Korean Journal of Microbiology
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    • v.35 no.4
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    • pp.277-282
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    • 1999
  • A bacterium producing the extracellular cellulase was isolated from cattle feces and screened as cellulase activity was excellent upon congo red straining method and activity measurements. Isolate was identified as Bacillus subtilis CH-10 on the basis of morphological and biochemical properties as well as cellular fatty acids composition. The enzyme which the isolate secretes had the optimum initial pH and temperature for its induction was 7.5 and 50${\circ}C$, respectively. The maximum CMCase activity in crude enzyme solution was observed at pH 7.5 and 75${\circ}C$ and was stable for pH 7.5 to 9.0 to maintain 70% activity. When the isolate was cultured in CMC media at 37${\circ}C$ for 24 hrs, CMCase and FPase activity was 1.13 U/㎖and 0.16U/㎖, respectively whereas Avicelase and ${\beta}$-glucosidase activity was not detected. When crude supernatant was used for zymogram, three major bands, cel 1, cel 2 and cel 3, were detected approximately 39, 41 and 57 KDa, respectively on CMC-SDS-PAGE.

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Electrical Properties of Transparent Conductive Films of Single-Walled Carbon Nanotubes with Their Purities

  • Lee, Seung-Ho;Goak, Jeung-Choon;Lee, Chung-Yeol;Lee, Nae-Sung
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.06a
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    • pp.56-56
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    • 2010
  • Single-walled carbon nanotubes (SWCNTs) have attracted much attention as a promising material for transparent conducting films (TCFs), due to their superior electrical conductivity, high mechanical strength, and complete flexibility as well as their one-dimensional morphological features of extremely high length-to-diameter ratios. This study investigated three kinds of SWCNTs with different purities: as-produced SWCNTs (AP-SWCNTs), thermally purified SWCNTs (TH-SWCNTs), thermally and acid purified SWCNTs (TA-SWCNTs). The purity of each SWCNT sample was assessed by considering absorption peaks in the semiconducting ($S_{22}$) and metallic ($M_{11}$) tubes with UV-Vis NIR spectroscopy and a metal content with thermogravimetric analysis (TGA). The purity increased as proceeding the purification stages from the AP-SWCNTs through the thermal purification to the acid purification. The samples containing different contents of SWCNTs were dispersed in water using sodium dodecyl benzensulfate (SDBS). Aqueous suspensions of different purities of SWCNTs were prepared to have similar absorbances in UV-Vis absorption measurements so that one can make the TCFs possess similar optical transmittances irrespective of the SWCNT purity. Transparent conductive SWCNT networks were formed by spraying an SWCNT suspension onto a poly(ethyleneterephthalate) (PET) substrate. As expected, the TCFs fabricated with AP-SWCNTs showed very high sheet resistances. Interestingly, the TH-SWCNTs gave lower sheet resistances to the TFCs than the TA-SWCNTs although the latter was of higher purity in the SWCNT content than the former. The TA-SWCNTs would be shortened in length and be more bundled by the acid purification, relative to the TH-SWCNTs. For both purified (TH, TA) samples, the subsequent nitric acid ($HNO_3$) treatment greatly lowered the sheet resistances of the TCFs, but almost eliminated the difference of sheet resistances between them. This seems to be because the electrical conductivity increased not only due to further removal of surfactants but also due to p-type doping upon the acid treatment. The doping effect was likely to overwhelm the effect of surfactant removal. Although the nitric acid treatment resulted in the similar. electrical properties to the two samples, the TCFs of TH-SWCNTs showed much lower sheet resistances than those of the TA-SWCNTs prior to the acid treatment.

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Analysis of crown size and morphology, and gingival shape in the maxillary anterior dentition in Korean young adults

  • Song, Jae-Won;Leesungbok, Richard;Park, Su-Jung;Chang, Se Hun;Ahn, Su-Jin;Lee, Suk-Won
    • The Journal of Advanced Prosthodontics
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    • v.9 no.4
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    • pp.315-320
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    • 2017
  • PURPOSE. The aim of this investigation was to analyze the dimensions of clinical crowns and to classify the crown and the gingival type in the anterior teeth in Korean young adults. MATERIALS AND METHODS. Casts were obtained from 50 subjects ranging in age from 24 to 32. Measurements of length and width were made on the casts using a pair of digital calipers on the entire dentition. Crown thickness and papilla height were also measured and MDW/CL (mesiodistal width to clinical length) and CW/CL (cervical width to clinical length) ratios of the maxillary anterior teeth were calculated. The K-clustering method was used for CW/CL to classify the anterior tooth shape into three groups (tapered, ovoid, and square), and one-way analysis of variance and Duncan's post-hoc comparison were used to evaluate statistical significance between the groups. Pearson's correlation analysis was performed between tooth shape and papillary height (PH) to demonstrate the correlation between tooth shape and gingival morphological characteristics. RESULTS. The average length of the maxillary central incisors was 9.89 mm; the mesio-distal width was 8.54 mm; and the ratio of width/length was 0.86 in Korean young adults. The average bucco-palatal thickness of the central incisor was 3.14 mm at the incisal 1/3 aspect. Ovoid type was the most common tooth shape (48%), followed by square type (29%) and taper type (23%) in the central incisors of Korean young adults. Tooth shape and gingival type were correlated with each other. CONCLUSION. New reference data were established for tooth size in Korean young adults and the data show several patterns of tooth shape and gingival type. Clinicians should diagnose and treat based on these characteristics for better results in the Korean population.

EFFECTS OF MOUTH BREATHING ON FACIAL SKELETAL MORPHOLOGY (구호흡이 안모골격 형태에 미치는 영향)

  • Lee, Min-Jeong;Kim, Jae-Gon;Yang, Yeon-Mi;Baik, Byeong-Ju
    • Journal of the korean academy of Pediatric Dentistry
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    • v.39 no.4
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    • pp.339-347
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    • 2012
  • There still remains a controversial debate whether facial skeletal morphological differences exist between patients with nasal and mouth breathing habits. The aim of this study is to assess a relationship between over a period of time mouth breathing and facial skeletal morphology by analyzing lateral cephalometric radiographs of patients with nasal or mouth breathing habits. A total of 120 patients with skeletal class I, II, and III, who had undergone orthodontic diagnosis in department of pediatric dentistry - chonbuk national university, were chosen and their lateral cephalometric radiographs were analyzed. These patients were divided into six groups of 20, each with or without mouth breathing habits. The result of this study has not showed noticeable differences in cephalometric measurements between nasal and mouth breathing children of skeletal class I, II, and III (p > 0.05). However, when the groups were divided by age factor, mouth breathers of age 12 and older showed significant differences in cephalometrics such as decreased ramus height, maxillary retrusion, and clockwise pattern of mandible than children under age 12 (p < 0.05). In conclusion, a longer period of mouth-breathing habits in children displayed a greater chance of impaired facial growth.

Development of relational river data model based on river network for multi-dimensional river information system (다차원 하천정보체계 구축을 위한 하천네트워크 기반 관계형 하천 데이터 모델 개발)

  • Choi, Seungsoo;Kim, Dongsu;You, Hojun
    • Journal of Korea Water Resources Association
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    • v.51 no.4
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    • pp.335-346
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    • 2018
  • A vast amount of riverine spatial dataset have recently become available, which include hydrodynamic and morphological survey data by advanced instrumentations such as ADCP (Acoustic Doppler Current Profiler), transect measurements obtained through building various river basic plans, riverine environmental and ecological data, optical images using UAVs, river facilities like multi-purposed weir and hydrophilic sectors. In this regard, a standardized data model has been subsequently required in order to efficiently store, manage, and share riverine spatial dataset. Given that riverine spatial dataset such as river facility, transect measurement, time-varying observed data should be synthetically managed along specified river network, conventional data model showed a tendency to maintain them individually in a form of separate layer corresponding to each theme, which can miss their spatial relationship, thereby resulting in inefficiency to derive synthetic information. Moreover, the data model had to be significantly modified to ingest newly produced data and hampered efficient searches for specific conditions. To avoid such drawbacks for layer-based data model, this research proposed a relational data model in conjunction with river network which could be a backbone to relate additional spatial dataset such as flowline, river facility, transect measurement and surveyed dataset. The new data model contains flexibility to minimize changes of its structure when it deals with any multi-dimensional river data, and assigned reach code for multiple river segments delineated from a river. To realize the newly developed data model, Seom river was applied, where geographic informations related with national and local rivers are available.

The evaluation on the biological safety of diagnostic ultrasound using radiation-induced apoptosis in the external granular layer of mouse cerebellum (마우스 소뇌과립층의 apoptosis를 지표로 한 진단용 초음파의 안전성 검증)

  • Oh, Heon;Lee, Song-eun;Yang, Jung-ah;Jo, Sung-kee;Chung, Chi-young;Son, Chang-ho;Kim, Sung-ho
    • Korean Journal of Veterinary Research
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    • v.39 no.3
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    • pp.628-634
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    • 1999
  • We have studied, by a nonisotopic in situ end-labeling(ISEL) technique, frequency of apoptosis in the external granular layer(EGL) of the cerebellum of immature mice by ${\gamma}$-rays irradiation from $^{60}Co$ or diagnostic ultrasound exposure. The total number of normal cells and cells showing morphological features of apoptosis were counted. The frequency of apoptotic cells was expressed as a percentage of the total number of cells in EGL. The extent of changes following 200 cGy(1090 cGy/min) was studied at 2, 4, 6, 8, 12, or 24 hours after exposure. The maximal frequency was found 6~8 hours after exposure. The immature mice that received 18, 36, 54, 108, 198, 396 cGy of ${\gamma}$-rays or diagnostic ultrasound(7.5MHz, 4.2mW, $I_{SPTA}=7.9mW/cm^2$, $I_{SPTA}=114.3W/cm^2$) for 10 or 30 minutes were examined 6 hours after irradiation. Measurements performed after ${\gamma}$-ray irradiation showed a dose-related increase in apoptotic cells in each of the mice studied. The dose-response curves were analyzed by a linear-quadratic model ; frequency of apoptotic cell in the EGL was y = $(0.1349{\pm}0.01175)D$+$(-0.0001522{\pm}0.0000334)D^2$+0.048($r^2$ = 0.981, D = dose in cGy). In the experiment of ultrasound exposure, the frequency of apoptotic cell was $0.106{\pm}0.130$(10 minutes exposure) and $0.167{\pm}0.220$(30 minutes exposure). We estimated the relative dose of the yield from the experiment with ultrasound by substituting the yield from ultrasound exposure into the curve from the ${\gamma}$-irradiation. The relative dose of ultrasound exposure compared with ${\gamma}$-irradiation were 0.432 cGy(10 minutes exposure) and 0.885 cGy(30 minutes exposure). We have found that there is no evidence to indicate that diagnostic ultrasound involves a significant risk.

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A Survey Low Temperature Damage of Tea Tree at South Korea in 2011 (2011년 남부지방 차나무 저온 피해 조사)

  • Hwang, Jung-Gyu;Kim, Yong-Duck
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.4
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    • pp.246-253
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    • 2012
  • Despite frequent freezing injury to tea trees due low temperature, drought, and strong wind during wintertime, no comprehensive measurements have been taken. We selected and examined 9 locations in Hwagae-myeon and 4 places in Agyang-myeon, Hadong-gun, Gyeonsanggnam-do where low temperature damage had occurred between December 2010 and February 2011. Our objective is to examine the effect of frost damage on the morphological symptom and harvest of a tea tree exposed to a constant low temperature environment during wintertime. The results of our analyses on meteorological environment, tea leaf chromaticity, water content and trypan blue are as follows: (1) the number of days with temperature of $-10^{\circ}C$ or less, which were subject to frost damage to a tea tree were 8 and 13.6% during the winterization period in 2011; (2) the accumulated time was 1,308 minutes, and the longest duration at $-10^{\circ}C$ was 588 minutes from 21:08 p.m. 15 January to 7:30 a.m. $16^{th}$ January. The rainfall was only 104 mm which was 306 mm less than the previous year; (3) the lightness L values in 2011 were higher than in 2012 due to dehydration and necrosis by blue discoloration and red discoloration at all areas in chromaticity measurement; (4) the water content in a tea leaf in 2011 was higher than in 2012 due to low rainfall and strong wind, and almost no cell death phenomenon was observed from normal tea leaves subject to no low temperature stress in a trypan blue analysis; and (5) partial coloration due to cell death, however, took place in the leaves damaged by blue discoloration subject to low temperature stress, and most coloration due to cell death took place in the leaves damaged by red discoloration.

Morphological Analysis of the Sinus Lateral Wall Using Cone-Beam Computed Tomography (콘빔형 전산화단층영상을 이용한 상악동 측벽의 형태학적 분석)

  • An, Seo-Young;Kim, Yong-Gun
    • Journal of Dental Rehabilitation and Applied Science
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    • v.28 no.4
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    • pp.349-357
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    • 2012
  • The purpose of this study was to measure the thickness of the sinus lateral wall using cone-beam computed tomography (CBCT), and to find the most suitable vertical position for lateral window opening prior to sinus elevation. Fifty three patients requiring sinus elevation had CBCT scans acquired by CB MercuRay (Hitachi, Medico, Tokyo, Japan) from July, 2010 to June, 2012. The thickness of the sinus lateral wall was measured according to its vertical position against the sinus inferior border (SIB), and its mean was calculated through two repeated measurements. The thickness of the sinus lateral wall was more than 2 mm at 2 mm above the sinus inferior border (SIB+2), however, it was less than 2 mm at 3 mm above the sinus inferior border (SIB+3). In conclusion, it is recommended that the inferior border of lateral wall window be made 3 mm above the sinus inferior border during sinus elevation using the lateral approach considering the thickness of the sinus lateral wall.

Facile Chemical Growth of Cu(OH)2 Thin Film Electrodes for High Performance Supercapacitors (간단한 화학적 합성을 통한 고성능 슈퍼캐패시터용 수산화 구리 전극)

  • Patil, U.M.;Nam, Min Sik;Shinde, N.M.;Jun, Seong Chan
    • KEPCO Journal on Electric Power and Energy
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    • v.1 no.1
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    • pp.175-180
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    • 2015
  • A facile soft chemical synthesis route is used to grow nano-buds of copper hydroxide [$Cu(OH)_2$] thin films on stainless steel substrate[SS]. Besides different chemical methods for synthesis of $Cu(OH)_2$ nanostructure, the chemical bath deposition (CBD) is attractive for its simplicity and environment friendly condition. The structural, morphological, and electro-chemical properties of $Cu(OH)_2$ thin films are studied by means of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), cyclic voltammetry (CV) and galvanostatic charge-discharge (GCD) measurement techniques. The results showed that, facile chemical synthesis route allows to form the polycrystalline, granular nano-buds of $Cu(OH)_2$ thin films. The electrochemical properties of $Cu(OH)_2$ thin films are studied in an aqueous 1 M KOH electrolyte using cyclic voltammetry. The sample exhibited supercapacitive behavior with $340Fg^{-1}$ specific capacitance. Moreover, electrochemical capacitive measurements of $Cu(OH)_2/SS$ electrode exhibit a high specific energy and power density about ${\sim}83Wh\;kg^{-1}$ and ${\sim}3.1kW\;kg^{-1}$, respectively, at $1mA\;cm^{-2}$ current density. The superior electrochemical properties of copper hydroxide ($Cu(OH)_2/SS$) electrode with nano-buds like structure mutually improves pseudocapacitive performance. This work evokes scalable chemical synthesis with the enhanced supercapacitive performance of $Cu(OH)_2/SS$ electrode in energy storage devices.

Environmental Change of Suspended Sediment Discharge by Human Action (인간활동으로 인한 부유토양유출의 환경변화)

  • 박종관
    • Journal of Environmental Science International
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    • v.2 no.2
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    • pp.153-160
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    • 1993
  • The problem of supply and transport of sediment from a mountainous catchment is very important in explaining dynamic geomorphology and the hydrological cycle. The discharge of suspended sediment is determined by a morphological system. Human interference to environment Is also an important, not negligible factor in sediment production. Moreover, growing concern in recent years for the problems of nonpoint pollution and for the transport of contaminants through terrestrial and aquatic ecosystems has highlighted the role of sediment-associated transport in fluvial systems. This study was conducted in forested and quarried catchments in order to clarify the different discharge process and the mechanism of suspended sediment dynamics for each catchment. As a forested catchment, the Yamaguchi River catchment which drains a $3.12km^2$ area was chosen. On the other hand, the Futagami River basin which is formed by three subbasins (1.07, 1.59 and $1.78km^2$), as a quarried catchment was selected. These catchments are situated to the north and east of Mt. Tsukuba, Ibaraki, Japan. The discharge pattern of suspended sediment from the Futagami River basin is more unstable and irregular than that from forested catchment, the Yamaguchi River catchment. Under the similar rainstorm conditions, suspended sediment concentration from quarried catchment during a rainstorm event increases from 43 to 27,340 mg/l. However, in the case of the forested catchment it changes only from nearly zero to 274 mg/l. Generally, the supply source of suspended sediment is classified into two areas, the in-channel and non-channel source areas. As a result of field measurements, in the case of the forested catchment the in-channel (channel bed, channel bank and channel margin) is the main source area of suspended sediment. On the other hand, remarkable sediment source area on the Quarried catchment is the non-channel that is unvegetated ground.

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