• Food Science of Animal Resources
• /
• v.35 no.1
• /
• pp.86-90
• /
• 2015

In this study, we reported the morphogenetic analysis and genome sequence by genomic analysis of the newly isolated staphylococcal phage SMSAP5 from soil of slaughterhouses for cattle. Based on transmission electron microscopy evident morphology, phage SMSAP5 belonged to the Siphoviridae family. Phage SMSAP5 had a double-stranded DNA genome with a length of 45,552 bp and 33 % G+C content. Bioinformatics analysis of the phage genome revealed 43 open reading frames. A blastn search revealed that its nucleotide sequence shared a high degree of similarity with that of the Staphylococcus phage tp310-2. In conclusion, this study is the first report to show the morphological features and the complete genome sequence of the phage SMSAP5 from soil of slaughterhouses for cattle.

• Food Science of Animal Resources
• /
• v.37 no.6
• /
• pp.884-888
• /
• 2017

In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods.

• ALGAE
• /
• v.21 no.3
• /
• pp.283-286
• /
• 2006

This study carried out phylogenetic analysis of dinoflagellate Gonyaulax polygramma which was responsible for a harmful algal bloom episode in Korea in 2004. Molecular phylogenetic tree inferred from the partial LSU rDNA data showed that G. polygramma came up among the monophyletic Gonyaulax clade, but did not have apparent genetic affiliation to other Gonyaulax species. This result appears to be consistent with characteristic morphological features of G. polygramma such as epitheca sharply tapering to the apex and thecal plates ornamented with numerous longitudinal striations.

• Mycobiology
• /
• v.37 no.1
• /
• pp.5-9
• /
• 2009

An Ophiostoma fungus was isolated from a stump of Pinus thunbergii in a forest on the West coast of Korea. Microscopic analysis using a light microscope, a stereo microscope, and a scanning electron microscope revealed that it had morphological features of Pesotum and Sporothix synanarmorphs. Based on the $\beta$-tubulin gene sequence analysis, the fungus was identified as the anamorph of Ophiostoma floccosum. Mycological properties of the species including its growth properties on different culture media were described.

• Applied Microscopy
• /
• v.19 no.2
• /
• pp.17-26
• /
• 1989

The cells of Campylobacter jejuni treated with physical or chemical disinfection agents were comparatively examined by scanning and transmission electron microscopies for their morphological features and internal ultrastructures. The normal cells of C. jejuni, showed typical spiral rod shapes. The ribosomes, nucleoids, and other cellular constituents were observed to be distributed evenly throughout the cytoplasm. The cells treated with heat or UV-light were changed to spherical or irregular shapes and their cell envelopes were destroyed to form ghost cells by liberating their cytoplasmic components. The cells treated with chlorine or monochloramine were also changed into irregular round shapes. The chlorinated cells showed very rough surface structures with many blob-like protrusions, while the surface of the monochloramine-treated cells appeared to be relatively smooth.

• Korean Journal of Veterinary Pathology
• /
• v.1 no.2
• /
• pp.153-156
• /
• 1997

This is the first recorded case of Sarcoptes scabiei in a Korean female raccoon confirmed by light and scanning electron microscopies. Grossly the skin on the abdomen head and legs was markedly thickened with alopecia and encrustation. histopathologically skin showed epidermal hyperplasia with spongiotic degeneration and parakeratosis. Ectoparasites were present in superficial and deep epidermal layers. Small numbers of leukocytes predominantly neutrophils were associated with the parasitic infestation. Scanning electron microscopy examination of affected skin scraping demonstrated round to oval parasitic mites measuring approximately 180 -250 x 220 - 350 um in size. The mites possessed four pairs of legs of which the two anterior pairs appeared relatively long with unjointed stalks while the two posterior leg pairs were rudimentary. these morphological features were consistent with Sarcoptes scabiei.

• Animal Systematics, Evolution and Diversity
• /
• v.34 no.1
• /
• pp.64-68
• /
• 2018

The genus Callochiton Gray, 1847 is small to medium sized chiton (up to $55{\times}36mm$ in size) and includes approximately 30 species worldwide, most of which are found on hard substrata in subtidal zone. To date, only three species of Callochiton Gray, 1847 have been reported in Japan. In this study, we found C. foveolatus(Is. Taki, 1938) as first record of the family Callochitonidae Plate, 1901 in Korea and its morphological features were described and compared with other northwestern Pacific species. In addition, the partial fragment of mitochondrial DNA cytochrome c oxidase subunit I sequences of C. foveolatus was determined as DNA barcoding record and compared with other congeneric species.

• Journal of Chest Surgery
• /
• v.21 no.1
• /
• pp.82-86
• /
• 1988

Tetralogy of Fallot with subarterial ventricular septal defect is known to be relatively common in the Far East. Among the 140 consecutive patients who underwent repair for tetralogy, 15 [10,7@] had this variant form of tetralogy. Though the degree of pulmonic stenosis seems only mild or moderately severe because of the absence of infundibular septum and relatively large size of pulmonary arteries in this type of tetralogy, it has a special surgical implication that a patch widening of the right ventriculotomy is necessary in every case to avoid the postoperative subpulmonic stenosis. A morphological and surgical features of tetralogy with subarterial VSD are presented.

• Mycobiology
• /
• v.34 no.2
• /
• pp.56-60
• /
• 2006

Higher Fungi were collected. twice a month from May to September 2004 during field survey trips to Seoraksan and Odaesan National Parks. All the collected specimens were investigated for the morphological characters of carpophores and other features, and deposited in the herbarium of the Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University, Chuncheon. Among the identified specimens, three genera Rhodotus, Hotermannia and Sebacina and four species Rhodotus palmatus, Gomphus clavatus, Holtermannia corniformis and Sebacina incrustans were confirmed as new to Korea and reported here with descriptions.

• Journal of Applied Biological Chemistry
• /
• v.43 no.2
• /
• pp.74-78
• /
• 2000

A strain that produces a high level of carboxymethyl cellulase was isolated from rotten leaves. The isolated strain was revealed to be gram-negative, oxidase-positive, and catalase-negative. From the electron microscopic features, it was identified as a rod-shaped bacterium with peritrichous flagella and did not form spores. Morphological and biochemical characteristics of the strain were found to be similar to the Pseudomonas species. However, carbon utilization test showed different results. Based on the results, this new strain was identified as Pseudomonas sp. CMC-50. CMCase produced by this strain showed a strong activity in neutral and weak acidic conditions and maximum activity at $50^{\circ}C$.