• Journal of Advanced Marine Engineering and Technology
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• v.23 no.1
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• pp.79-87
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• 1999

The most part of the faculty drop a trouble and damage of machine equipment even if whatever cause they break out take place at local and trifling place and the factor dominating their trouble is due to wear debris occurred in the lubricated machine moving surface. This study has been car-ried out to identify morphology of wear debris on the lubricated machine moving system by means of computer image analysis. Namely the wear debris contained in lubricating oil extracted from movable machine equipment will be filtered through membrane filter(void diameter 0.45${\mu}m$) and will be analyzed with its data information such as 50% volume diameter aspect roundness and reflectivity. Morphological characteristic of wear debris is easily distinguished by four shape parameters it is necessary to divide small class of every 100 wear debris in total wear particles in order to distinguish morphological characteristic of wear debris more easily by computer image analysis. We are sure that operation condition diagnosis of the lubricated machine moving surfaces is possible by computer image analysis.

• Tribology and Lubricants
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• v.17 no.1
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• pp.16-21
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• 2001

The wear particles is released from the moving surfaces in gear systems of transmission and its morphology is directly related to the damage and failure to gear system from which the particles originated. It is the effective method for damage condition estimation of automobile transmission gear to observe wear debris in gear oil. We tested with new transmission and took out gear oil according to driving distance. To be applied to damage condition of gear system in transmission of automobile,4 shape parameters of wear particles in gear oil were calculated and wear volume were presumed with the image processing system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.4
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• pp.411-434
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• 1982

Yield loss of wheat and barley due to meteorological constraints has been analyzed in order to get the basic information, which will lead to the counter-measures for dissemination of agricultural technology and administration. These meteorological damages were analyzed on the results of percentage yield loss and mechanism of damage and the aspects of constraints were explained. The annual yield loss of wheat and barley were 21.7% by meteorological stress: Cold damage, 5.9% ; excessive soil moisture, 5.6% ; lodging, 2.9% ; drought, 3.0% ; disease, 4.3% etc. Those damages by the stresses mentioned above and rain damage were analyzed in relation to the growth stages and the degrees of damage. The predispositions and the growth of wheat and barley to those meteorological stress are also discussed. Varietal resistances of wheat and barley to those stresses were indexed and the physiological and morphological characteristics of these resistant cultivars are described. Cultural practices to minimize the damages were also reviewed.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2003.10a
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• pp.42-45
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• 2003

Nondestructive damage sensing and mechanical properties for acid-treated carbon nanotube (CNT) and nanofiber (CNF)/epoxy composites were investigated using electro-micromechanical technique and acoustic emission (AE). Carbon black (CB) was used to compare to CNT and CNF. The results were compared to the untreated case. The fracture of carbon fiber was detected by nondestructive acoustic emission (AE) relating to electrical resistivity under double-matrix composites test. Sensing for fiber tension was performed by electro-pullout test under uniform cyclic strain. The sensitivity for fiber damage such as fiber fracture and fiber tension was the highest for CNT/epoxy composites. Reinforcing effect of CNT obtained from apparent modulus measurement was the highest in the same content. For surface treatment case, the damage sensitivity and reinforcing effect were higher than those of the untreated case. The results obtained from sensing fiber damage were correlated with the morphological observation of nano-scale structure using FE-SEM. The information on fiber damage and matrix deformation and reinforcing effect of carbon nanocomposites could be obtained from electrical resistivity measurement as a new concept of nondestructive evaluation.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.58-64
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• 2020

This present study was conducted to investigate protective effect of discontinuous Percoll gradient containing alpha-linolenic acid (ALA) before freezing process on viability, acrosome damage, mitochondrial activity, and oxidative stress of frozen-thawed boar spermatozoa. The separation of spermatozoa by discontinuous Percoll gradient was performed by different concentration of Percoll solution (45/90%) containing ALA combined with bovine serum albumin (BSA), and collected sperm in each Percoll layer was cryopreserved. To evaluate viability, acrosome damage, mitochondrial activity, and reactive oxygen species (ROS) level of frozen-thawed sperm, flow cytometry was used. Morphological abnormalities were observed under light microscope. In results, viability of sperm from 90% Percoll layer was higher than control and 45% Percoll group (p < 0.05). Separated sperm in 90% Percoll layer had lower acrosome damage and morphological abnormalities than control as well as viability, whereas 45% Percoll group was higher (p < 0.05). Similar with acrosome damage and abnormalities, mitochondrial activity was slightly enhanced and the population of live sperm with high ROS level was decreased by 90% Percoll separation, however, there was no significant difference. Supplementation of 3 ng/mL ALA into Percoll solution increased sperm viability and decreased population of live sperm with high ROS compared to control (p < 0.05). In conclusion, discontinuous Percoll gradient before freezing process could improve efficiency of cryopreservation of boar sperm through selection of sperm with high freezing resistance, and supplement of ALA during Percoll gradient might contribute suppression of ROS generation via stabilizing of plasma membrane during cryopreservation.

• ALGAE
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• v.20 no.4
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• pp.353-361
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• 2005

Ascophyllum nodosum (L.) Le Jolis has a systemic infection with the ascomycete Mycophycias ascophylli (Cotton) Kohlmeyer and Volkmann-Kohlmeyer with which it establishese a mutualistic symbiosis. In addition, A. nodosum is the host for the obligate red algal epiphyte, Vertebrata lanosa (L.) Christensen. Using light and electron microscopy we describe morphological and cytochemical changes occurring as a consequence of rhizoid penetration of V. lanosa into cortical host tissue. Rhizoids induce localized cell necrosis based on physical damage during rhizoid penetration. Host cells adjacent to the rhizoid selectively undergo a hypersensitive reaction in which they become darkly pigmented and become foci for hyphal development. Light and electron microscopy show that M. ascophylli forms dense hyphal aggregations on the surface of the V. lanosa rhizoid and extensive endophytic hyphal growths in the rhizoid wall. This is the first morphological evidence of an interaction between M. ascophylli and V. lanosa. We speculate that M. ascophylli may be interacting with V. lanosa to limit tissue damage to their shared host. In addition, the fungus provides a potential pathway for the transfer of materials (e.g., nutrients and photosynthate) between the two phototrophs.

• Korean Circulation Journal
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• v.48 no.12
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• pp.1135-1144
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• 2018

Background and Objectives: Mitochondria play a key role in the pathophysiology of heart failure and mitochondrial permeability transition pore (MPTP) play a critical role in cell death and a critical target for cardioprotection. The aim of this study was to evaluate the protective effects of cyclosporine A (CsA), one of MPTP blockers, and morphological changes of mitochondria and MPTP related proteins in monocrotaline (MCT) induced pulmonary arterial hypertension (PAH). Methods: Eight weeks old Sprague-Dawley rats were randomized to control, MCT (60 mg/kg) and MCT plus CsA (10 mg/kg/day) treatment groups. Four weeks later, right ventricular hypertrophy (RVH) and morphological changes of right ventricle (RV) were done. Western blot and reverse transcription polymerase chain reaction (RT-PCR) for MPTP related protein were performed. Results: In electron microscopy, CsA treatment prevented MCT-induced mitochondrial disruption of RV. RVH was significantly increased in MCT group compared to that of the controls but RVH was more increased with CsA treatment. Thickened medial wall thickness of pulmonary arteriole in PAH was not changed after CsA treatment. In western blot, caspase-3 was significantly increased in MCT group, and was attenuated in CsA treatment. There were no significant differences in voltage-dependent anion channel, adenine nucleotide translocator 1 and cyclophilin D expression in western blot and RT-PCR between the 3 groups. Conclusions: CsA reduces MCT induced RV mitochondrial damage. Although, MPTP blocking does not reverse pulmonary pathology, it may reduce RV dysfunction in PAH. The results suggest that it could serve as an adjunctive therapy to PAH treatment.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.40 no.6
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• pp.574-581
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• 1991

Damage in film capacitors has been investigated, using FTIR and ESCA, aiming to elucidate the nature of electrode removal and the possibility of base films to be damaged. Also, tests were conducted to investigate the effect of a long-term thermal aging at elevated temperatures. Unsuccessful clearing or grape-clustering processes can induce a long-term degradation which involves the chemical and morphological changes. Major changes are the oxidation and the decrease in surface crystallinity possibly arising from the corona discharge. An immediate deterioration of BOPP film may occur when the air entrapped between the film layers induces an extensive autocatalytic oxidative degradation. This type of immediate damage may result in a premature failure at an early stage of qualification test. As far as the nature of electrode removal is concerned, a permanent removal of electrode materials was observed in the main erosion area.

• Biomedical Science Letters
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• v.10 no.3
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• pp.259-262
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• 2004

The aim of present study was to examine the effect of carrageenan on the tendon of skeletal muscle of rat. The tendon damage was induced by injection of carrageenan into skeletal hind muscle of rats. Rats were killed on 48 hours after carrageenan injection. The resulting tendons were fixed with 10％ neutral buffered formalin (NBF), dehydrated, embedded, sectioned by 4 μm, and stained by phosphotungstic acid hematoxylin (PTAH) or hematoxylin-eosin (H-E). Carrageenan induced the segregation of tendon fibers, intratendinous cleft, segregation of muscle cell group, wave arrangement of tendon fiber. The results suggest that carrageenan induced tendon damage of rat's skeletal muscle by morphological changes.

• Tribology and Lubricants
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• v.16 no.5
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• pp.389-394
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• 2000

Scanning Probe Microscope (SPM) such as Scanning Tunneling Microscope (STM) and Atomic Force Microscope (AEM) was shown to be the powerful tool for nano-scale characterization of material surfaces. Using this technique, surface morphology of the cyclically deformed Cu or Cu-Al single crystal was observed. The surface became proportionately rough as the number of cycles increased, but after some number of cycles no further change was observed. Slip steps with the heights of 100 to 200 nm and the widths of 1000 to 2000 nm were prevailing at the stage. The slipped distance of one slip system at the surface was not uniform, and formation of the extrusions or intrusions was assumed to occur such place. By comparing the morphological change caused by crystallographic orientation, strain amplitude, number of cycles or stacking fault energy, some interesting results which help to clarify the basic mechanism of fatigue damage were obtained. Furthermore, applicability of the scanning tunneling microscopy to fatigue damage is discussed.