• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권6호
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• pp.515-525
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• 2005

Purpose of study: Lingual nerve damage can be caused by surgery or trauma such as physical irriatation, radiation, chemotherapy, infection and viral infection. Once nerve damage occurred, patients sometimes complain taste change and loss of taste along with serious disturbance of tongue. The purpose of this study was to evaluate the effects of unilateral lingual nerve transection on taste as well as on the maintenance of taste buds. Materials & Methods: Male Sprague-Dawley rats weighing 220-250g received unilateral transection of lingual nerve, subjected to the preference test for various taste solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) with two bottle test paradigm at 2, 4, 6, or 8 weeks after the operation. Tongue was fixed with 8% paraformaldehyde. After fixation, they were observed with scanning electron microscope(JSM-$840A^{(R)}$, JEOL, JAPAN) and counted the number of the dorsal surface of the fungiform papilla for changes of fungiform papilla. And, Fungiform papilla were obtained from coronal sections of the anterior tongue(cryosection). After cryosection, immunostaining with $G{\alpha}gust$(I-20)(Santa Cruz Biotechnology, USA), $PLC{\beta}2$(Q-15)(Santa Cruz Biotechnology, USA), and $T_1R_1$(Alpha Diagnostic International, USA) were done. Immunofluorescence of labeled taste bud cells was examined by confocal microscopy(F92-$300^{(R)}$, Olympus, JAPAN). Results: The preference score for salty and sweet tended to be higher in the operated rats with statistical significance, compared to the sham rats. Fungiform papilla counting were decreased after lingual nerve transaction. In 2 weeks, maximum differences occurred. Gustducin and $T_1R_1$ expressions of taste receptor in 2 and 4 weeks were decreased. $PLC{\beta}2$ were not expressed in both experimental and control group. Conclusion: This study demonstrated that the taste recognition for sweet and salty taste changed by week 2 and 4 after unilateral lingual nerve transection. However, regeneration related taste was occurred in the presence of preserving mesoneurial tissue and the time was 6 weeks. Our results demonstrated that unilateral lingual nerve damage caused morphological and numerical change of fungiform papilla. It should be noted in our study that lingual nerve transection resulted in not only morphological and numerical change but also functional change of fungiform papillae.

• Archives of Pharmacal Research
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• 제23권6호
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• pp.613-619
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• 2000

We investigated the expression profiles of rat fibrotic liver induced by bile duct ligation and scission (BDL/S) using the 3'-directed cDNA libraries. The possibility that the 3'-directed cDNA library represents the mRNA population faithfully was examined by northern blots. During the northern analysis based on fibrotic liver expression profile, we found for the first time that purine specific sodium nucleoside cotransporter (SPNT) was upregulated in BDL/S-induced fibrotic liver. To determine whether the accumulation of bile juice could affect the expression of SPNT mRNA or not, we examined the change of SPNT mRNA expression at 3, 14, 28 days after BDL/S operation. No change in SPNT expression was observed in rat liver at 3 days after surgery. In contrast, there were significant increases in SPNT expression at 14 and 28 days after surgery. We also examined whether chronic liver damage affected SPNT mRNA expression. SPNT mRNA level was significantly increased in BDL/S-induced fibrotic rat liver, whereas no significant change was obserbed in fibrotic livers chronically exposed to carbon tetrachloride or dimethylnitrosamine. From the above results, although further study might be needed, it was considered that the increment of SPNT mRNA in BDL/S liver morphological compatibility to human was remarkable.

• Journal of Photoscience
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• 제7권4호
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• pp.161-167
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• 2000

Temperature-dependent electroretinogram responses were investigated in the dark adapted bullfrog eyes within the physiological temperature range 0-40$\^{C}$. In hypothermic process(25→0→25$\^{C}$), the amplitude of b-and c-wave decreased with lowering the temperature again. Both b-wave amplitude and threshold responses were maximal around 15$\^{C}$ during the temperature increment. Upon warming to room temperature again (25$\^{C}$), the b-wave amplitude was approximately doubled as compared to that of control without temperature changes. During the hyperthermic process (25→40→25$\^{C}$), however, the responses decreased with warming, and the wave amplitude failed to recover by cooling to 25$\^{C}$ again. As describe above, the recoveries of ERG in both processes show the striking difference. The hypothermia induces the amplification of the b-wave, that is, enhances the retinal function with the temperature recovery toward room temperature. While the hypertherima produces the decrease of the b-wave even though recovered to room temperature, which indicates an irreversible retina. The morphological alteration is shown both hypothermic and hyperthermic process, such as an appearance of large vacuoles and degenerating outer segments, more intense in hyperthermia, similar to light induced damage.

• Journal of Photoscience
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• 제9권2호
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• pp.454-456
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• 2002

It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.

• 동의생리병리학회지
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• 제21권1호
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• pp.214-218
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• 2007

The water extracts of Samultang (Samul) has been used for treatment of ischemic heart and brain damage in Oriental traditional medicine. However, little is known about the mechanism by which the water extract of Samul rescues cells from oxidative damages in cisplatin-induced ototoxicity. Cisplatin is a widely used chemotherapeutic agent that is also highly ototoxic. This study was designed to investigate the protective effects of Samul on ciplatin-induced ototoxicity in HEI-OC1 auditory cells and organ of Corti explant culture. Cisplatin markedly decreased the viability of HEI-OC1 auditory cells. However, treatment of HEI-OC1 cells with Samul significantly reduced cisplatin-induced cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Cisplatin induced cytotoxicity in isolated and cultured hair cell progenitors from postnatal rat cochleae. These progenitor cells are isolated from the lesser epithelial ridge (LER, or outer spiral sulcus cell) area of pre-plated neonatal rat cochlear segments. However, Samul completely protected the morphological changes of organ of Corti and LER. Taken together, these data suggest that the protective effects of the water extracts of Samul against cisplatin may be mediated by the reduction of intracellular peroxide generation.

• 대한공간정보학회지
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• 제21권4호
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• pp.37-44
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• 2013

최근 기상 이변에 따라 단시간에 집중되는 돌발홍수에 의해 해마다 막대한 인적, 물적 피해를 입고 있다. 이러한 국지성호우에 의한 산지하천이나 미소하천에서 첨두유출량을 예측하기 위한 도구로서 GIS를 적용하고 있는 추세이다. 하지만 수문학적 접근이 주를 이루고 있으며 GIS를 이용한 지형분석으로의 접근은 매우 미비한 실정이다. 본 연구에서는 돌발홍수를 발생시키는 강우량을 GIS기법과 GcIUH의 모형을 연계하여 산정하였고, 유역별 GcIUH 매개변수를 추출하여 한계유량에 따른 GcIUH 매개변수간의 상관관계를 분석하였다.

• 한국응용곤충학회지
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• 제54권2호
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• pp.137-144
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• 2015

식물은 유도저항성이나 천적류의 공격활동 변화를 유발하여 관련 초식성 곤충류 간 상호작용관계에 불리한 영향을 미칠 뿐만 아니라 식물체내 영양물질 동태나 타감물질 그리고 외부 형태적 변화 등을 통하여 초식곤충류에 유리한 영향을 미치기도 한다. 본고에서는 식물이 개재되었을 때 초식 곤충류간의 상호작용 관계와 초식성 곤충 가해에 대한 식물의 여러 가지 반응특성과의 관계나 곤충류 생활사 특성이 초식성 곤충군집의 구조에 미치는 영향을 살펴보고 그의 군집학적 의의를 고찰하였다.

• 한국염색가공학회지
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• 제20권2호
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• pp.59-65
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• 2008

The tannin acid and the enzymes have been used in order to improve the ruggedness in laundry and the absorption of dyes and pigments in the textile industry for several years. The enzyme processing on the protein fiber minimizes the damage of the entire fiber and improves the dyeability by effectively modifying only the hydrophobic surface. This study tried out the structural observation by applying the Castanea crenata sieb. et. zucc. containing abundant tannin to the hair dyeing as the natural dyeing pigment along with Protease of Rhizopus sp. The dyeability was improved as compared to the dyeing using only the synthetic tanning and iron mordant. When the depth of pigment was higher in accordance with the surface observation, the enzyme dissolution had impact on dyeing and so the keratin layer on the hair surface. Accordingly, it was found that the appropriate depth was between 0.01 and 0.03%. It was estimated that 0.1% protease would treated within 30min. Consequently, it would cause the good reaction with the functional group of tannin pigment.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.275-279
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• 2002

Change Detection is a useful technology that can be applied to various fields, taking temporal change information with the comparison and analysis among multi-temporal satellite images. Especially, Change Detection that utilizes high-resolution satellite imagery can be implemented to extract useful change information for many purposes, such as the environmental inspection, the circumstantial analysis of disaster damage, the inspection of illegal building, and the military use, which cannot be achieved by low- or middle-resolution satellite imagery. However, because of the special characteristics that result from high-resolution satellite imagery, it cannot use a pixel-based method that is used for low-resolution satellite imagery. Therefore, it must be used a feature-based algorithm based on the geographical and morphological feature. This paper presents the system that builds the change map by digitizing the boundary of the changed object. In this system, we can make the change map using manual or semi-automatic digitizing through the user interface implemented with a floating window that enables to detect the sign of the change, such as the construction or dismantlement, more efficiently.

• Tribology and Lubricants
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• 제22권5호
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• pp.276-281
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• 2006

The fractal dimension is quantitatively to define the irregular characteristic of the shape in natural. It can be useful in describing morphological characteristics of various wear particles. This paper was undertaken to diagnose failure condition for sliding members in lubrication by fractal dimension. It will be possible to diagnose wear mechanism, friction and damage state of machines through analysis of shape characteristics for wear particle on driving condition by fractal parameters. In this study, the calculating and analyzing methods of fractal dimensions were constructed for the condition monitoring and wear particle analysis in lubricant condition. So, we carried out the Friction and wear test with the ball on disk type tester, and the fractal parameters of wear particle in lubricated conditions were calculated. Fractal parameters were defined as texture fractal dimension ($D_{t}$), structure fractal dimension ($D_{s}$) and total fractal dimension (D).