• 치과방사선
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• 제13권1호
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• pp.151-162
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• 1983

The author analyzed the morphologic changes of bone structure from 848 radiograph is (424 joints) of 212 patients with temporomandibular joint arthrosis, which were obtained by the oblque-Iateral transcranial projection and orthopantomography. The interelation of the bone changes and condylar head positions the results were as follows: 1. In the 212 patients with TMJ arthrosis, 210 patients(99.05%) show the condylar positional changes. Among them, 187 patients 989.05%) show the bone changes. 2. In TMJ arthrosis patients with bone changes, 10% patients(57.75%) show both the condylar positional changes and bone changes. 66 patients( %) show the condylar psoitional changes bilaterally and bone changes unilaterally. On the other hand, 11 patients (5.88%) show the condylar positional changes unilaterally and bone change bilaterally. 3. The bone changes in the TMJ arthrosis patients with the condylar positional changes were as follows: There were the flatlening of articular surface in 103 cases (26.55%) the erosion in 99cases 925.57%), and the erosion in 88 cases (22.68%). There were not much differences among the three types of bone changes. And the deformity in 70 cases (18.04%), the sclerosis in 22 cases(5.67%), the marginal protiferation in 6 cases(1.55%) were seen. 4. The regions of bone changes in TMJ arthrosis patients with condylar positional changes were as follows: They occurred at the condyle head(51.04%), the articular eminence(39.20%) and the articular fossa(9.60%) in that order. The condylar positional changes and bone changes according to the regions were as follows: a) In the bone changes at the condyle head, the flatteming (34.63%) was a most frequent finding and the deformity(27.63%) the erosion(24.32%) in the order. In the condylar positional changes, the downward positioning of condyle(41.44%) was a most frequent finding in the mouth closed state and the restricted movement within the articular fossa(35.46%) in the mouth open state. b) In the bone changes at the articular eminence, the eburnation(33.26%) was a most frequent finding and the flatteming(31.16%), the erosion(28.37%) in that order. In the condylar positional changes, the downward positionirg of condyle(39.81%) was a most frequent finding in the mouth closed state and the restricted movement within the articular fossa(24.77%) in the mouth open state. c) In the bone changes at the articular fossa, the eburnation(72.90%) was amost frequent finding and theerosion(17.76%), the sclerosis(9.35%) in that arder. In the condylar positional changes, the downward positionirg of condyle(41.5%) was a most frequent finding in the mouth closed state and the mormal positionirg of condyle(27.78%) in the mouth open state.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.551-557
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• 2015

Background: Differentiating morphologic features based on hematoxylin-eosin (HE) staining is the most common method to classify pathological subtypes of non-small-cell lung cancer (NSCLC). However, its accuracy and inter-observer reproducibility in pathological diagnosis of poorly differentiated NSCLC remained to be improved. Materials and Methods: We attempted to explore the role of immunohistochemistry (IHC) staining in diagnosing pulmonary squamous cell carcinoma (SQCC) with poorly differentiated features by HE staining or with elevated serum adenocarcinoma-specific tumor markers (AD-TMs). We also compared the difference of epidermal growth factor receptor (EGFR) mutation rate between patients with confirmed SQCC and those with revised pathological subtype. Logistic regression analyses were used to test the association between different factors and diagnostic accuracy. Results: A total of 132 patients who met the eligible criteria and had adequate specimens for IHC confirmation were included. Pathological revised cases in poor differentiated subgroup, biopsy samples and high-level AD-TMs cases were more than those with high/moderate differentiation, surgical specimens and normal-level AD-TMs. Moreover, biopsy sample was a significant factor decreasing diagnostic accuracy of pathological subtype (OR, 4.037; 95% CI 1.446-11.267, p=0.008). Additionally, EGFR mutation rate was higher in patients with pathological diagnostic changes than those with confirmed SQCC (16.7% vs 4.4%, p=0.157). Conclusions: Diagnosis based on HE staining only might cause pathological misinterpretation in NSCLC patients with poor differentiation or high-level AD-TMs, especially those with biopsy samples. HE staining and IHC should be combined as pathological diagnostic standard. The occurrence of EGFR mutations in pulmonary SQCC might be overestimated.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.201-208
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• 2013

Objective: To investigate changes in the invasive capacity of gastric cancer cells in vitro after expression inhibition of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) and underlying mechanisms. Methods: Using adhesion selection, two subpopulations with high ($M_H$) or low ($M_L$) invasive capacity were separated from the human gastric cancer cell line MKN-45 ($M_0$). Tiam 1 antisense oligodeoxynucleotide (ASODN) was transfected into $M_H$ cells with liposomes, and expression of Tiam 1 mRNA and protein was determined by RT-PCR and quantitative cellular-ELISA. Changes in the cytoskeleton, invasive capacity in vitro and expression of ras-related $C_3$ botulinum toxin substrate 1 (Rac 1), integrin ${\beta}1$ and matrix metalloproteinase 2 (MMP 2) between Tiam 1 ASODN transfected $M_H$ cells and non-transfected cells were observed by HE staining, cytoskeletal protein staining, scanning electron microscopy, Boyden chamber tests and cyto-immunohistochemistry. Results: A positive correlation existed between the expression level of Tiam l mRNA or protein and the invasion capacity of gastric cancer cells. After ASODN treatment ($0.43{\mu}M$ for 48 h), Tiam 1 mRNA transcription and protein expression in $M_H$ cells were decreased by 80% and 24% respectively (P < 0.05), compared with untreated controls, while invasive capacity in vitro was suppressed by 60% (P < 0.05). Morphologic and ultrastructural observation also showed that ASODN-treated $M_H$ cells exhibited smooth surfaces with obviously reduced filopodia and microspikes, which resembled $M_0$ and $M_L$ cells. Additionally, cytoskeletal distribution dramatically altered from disorder to regularity with reduced long filament-like structure, projections, pseudopodia on cell surface, and with decreased acitn-bodies in cytoplasm. After Tiam 1 ASODN treatment, the expression of Rac 1 and Integrin ${\beta}1$ in $M_H$ cells was not affected (P > 0.05), but that of MMP 2 in $M_H$ cells was significantly inhibited compared with untreated cells (P < 0.05). Conclusion: Over-expression of Tiam-1 contributes to the invasive phenotype of gastric cancer cells. Inhibition of Tiam 1 expression could impair the invasive capacity of gastric cancer cells through modulating reconstruction of the cytoskeleton and regulating expression of MMP 2.

• 대한구순구개열학회지
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• 제6권1호
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• pp.1-15
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• 2003

이차골 이식술을 시행 받은 구순 구개열을 가지는 성인 남자 20명의 두부계측방사선 사진을 McNamara씨 분석법을 중심으로 하여 계측한 결과는 다음과 같다. 구순 구개열 환자에서 Na. perpendicualr to point A칼은 글.98±5.70, SWh angle 값은 76.45±4.69, Pogonion to Na. perpendicular간은 -6.38± 6.73을 나타내었다. Effective maxillary length는 85.6±4.42, effective mandibular length 값은 123.88±7.10을 보였고 하악각은 29.9±5.09, facial axis angle은 5.53±2.03으로 관찰되었다. Point A에 대해 상악 전치의 돌출도는 3.95±2.74의 값을 보였다.

• 한국의학물리학회지:의학물리
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• 제19권3호
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• pp.178-185
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• 2008

간경변 및간암 환자의 증가로 간이식술의 필요성이 점점 증가되고 있고, 특히 공여자의 생체 간이식은 간이식술의 주된 분야를 차지하고 있으며 간이식 수술 전 공여자에서 간체적의 정확한 측정은 수술 후 공여자와 수여자의 간기능을 예측하는데 있어 중요한 자료가 되며, 성공적인 수술과 환자의 예후에 밀접한 영향을 미친다. 그러나 현재 환자의 간체적을 구하는 과정은 환자의 모든 CT 영상위의 간을 수작업을 통해 영상분할한 후에 3차원 간체적을 구하고 있으며 많은 시간과 노력이 필요한 작업이다. 이러한 문제를 해결하기 위해서 본 논문에서는 자동으로 간과 비장을 문턱값처리, 형태학적 영상처리, 3차원 영역확장법등의 기법을 이용하여 분할하는 알고리즘을 개발하여 체적을 구하는 시간을 단축하였다. 이러한 알고리즘의 정확성을 평가하기 위해서 동물의 실제 간과 비장을 팬텀으로 제작하여 실제 측정한 체적과 알고리즘으로 분할된 영상의 결과를 비교 평가하였다. 문턱치값의 설정에 따라 다른 결과를 보이는 특성이 있지만 자동으로 문턱치를 결정했을 때 비장과 간의 체적측정 오차는 9.27%, -4.52%이었으며, 수동으로 문턱치를 결정했을 때 최소 오차가 각각 0.2%, 0.17%의 결과를 보였다. 이러한 팬텀 연구를 통해 자동 분할 알고리즘으로 얻은 체적의 결과가 정확성과 재현성을 보여주어 추후 간체적을 구하는 보조수단으로 활용될 수 있을 것이라 예상된다.

• 대한치과보철학회지
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• 제45권4호
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• pp.492-505
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• 2007

Statement of problem: An orthodontic miniscrew implant has been used as a skeletal anchorage for orthodontic treatment. However, any relation among the influence of the cortical bone, morphologic differences of orthodontic miniscrew implants and new bone formation hasn't been made clear yet. Purpose: The purpose of this study was to evaluate whether the orthodontic miniscrew implant could work as an intraoral skeletal anchorage immediately and stably for orthodontic treatment after insertion of it. Material and methods: Two types of orthodontic miniscrew implants were used in this experiment; tapered type and straight type. One hundred and sixty eight orthodontic miniscrew implants were inserted into the tibiae of 21 rabbits and sacrificed on 3, 7, 11, 14, 21 and 28days later after insertion of them to study removal torque values and histologic and histomorphometric analyses. Results: The results were as follows. 1. The removal torque values of the tapered type were higher than those of the straight type in all groups(p<0.05). 2. There wasn't any distinguishing differences between the tapered type and the straight type about the new bone formation percentage. 3. The removal torque values for both the tapered type and the straight type were gradually decreased at early stages of the test but started to increase at the 7 days group of the straight type and the 11 days group of the tapered type. 4. New bone formation percentage was increased gradually for both the tapered and the straight types as time passed(p<0.05). 5. It was found that the tapered type showed lower values in the cortical bone about both the maximum equilibratory stress distribution and the maximum principal stress distribution than the straight type in linear finite elements analysis. Conclusion: According to the research, the removal torque values were decreased at 7 days group of the tapered type and 11 days group of the straight type after the insertion of the orthodontic miniscrew implants in tibiae of rabbits. Considering the human bone activity, it is better to apply the orthodontic force $3{\sim}4$ weeks later than to apply it immediately after the insertion of orthodontic miniscrew implants. Considering that general orthodontic force is about $250{\sim}500$ grams, the tapered type can be worked as a stable skeletal anchor age in an orthodontic treatment even if the orthodontic force is applied on it immediately after the insertion of it.

• 대한치과교정학회지
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• 제28권2호
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• pp.203-217
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• 1998

Sella는 오랜 동안 교정학에 있어 필수적인 landmark로서 중요한 위치를 차지해 왔다. 그러나 sella turcica는 그 안에 성장호르몬을 비롯한 많은 중요 호르몬을 분비하는 뇌하수체를 함유하고 있는 구조물로서 sella turcica의 크기는 뇌하수체의 크기를 비교적 정확히 반영한다고 알려져 있다. 따라서 만일 뇌하수체의 크기와 그 기능이 서로 비례한다면 두부방사선 사진에서 관찰되는 sella turcica의 크기와 성장호르몬을 비롯한 여러 뇌하수체 호르몬 분비 기전간에 밀접한 관련이 있을 것으로 추측되며 만일 그렇다면 악안면 성장에 중요한 역할을 하는 호르몬 분비 차이에 의한 악골부조화와 이로 인한 III급 부정교합과의 상관관계도 의심해 볼 수 있다. 따라서 본 연구에서는 50명의 정상교합자 남녀와 50 명의 III급 부정교합자 남녀를 대상으로 하여 측모 두부방사선사진과 전후방 두부방사선 사진에서 관찰되는 sella turcica의 부피를 구하고 두부방사선 분석을 통해 IIII 부정교합간의 상관 관계를 구하였다. 연구 결과 정상교합군보다 III급 부정교합군에서 sella turcica의 부피가 유의하게 큰 것으로 나타났으며 (p<0.001), III급 부정교합군에서 여성이 남성 보다 더 큰 sella turcica부피를 갖는 것으로 나타났다(p<0.05). 또한 III급 부정교합을 반영하는 APDI, ANB, effective mandibular length등의 항목에서 sella turcica부피와 높은 상관관계를 나타냈고 전두개저 길이에 대한 sella turcica 부피의 비를 표시하는 Sella Index 는 sella turcica 부피 자체보다 III급 부정교합을 더 정확히 반영하였다. 따라서 악안면 발육에 대한 성장호르몬의 효과나 sella turcica부피와 뇌하수체 호르몬 분비량과의 관계에 대한 지속적인 연구, sella turcica부피에 따른 악안면 성장에 대한 장기적인 연구를 바탕으로 Sella Index를 III급 부정교합을 예측하는 지표로서 활용할 수 있을 것이다.

• 대한치과교정학회지
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• 제23권4호
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• pp.503-527
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• 1993

This paper describes a new method to create an animal model for TMJ internal derangement in the New Zealand white rabbits and the light and electron microscopical changes of posterior attachment of them. Twenty six rabbits(2.5-3.0kg), four normal and twenty two experimental, were used. The right disc of experimental animal was displaced anteriorly without sectioning the posterior attachment and tied to the zygomatic arch with nylon not to be reduced to the original position. The left TMJ was sham-operated to be compared with its right experimental one. Normal animals were sacrificed one day and eight weeks after experiment. Experimental animals were sacrificed one day, ten days, three weeks, five weeks and eight weeks after surgery respectively. They were fixed intravenously with $2\%$ glutaldehyde under general anesthesia and the samples of them were processed for light and electron microscopic examination. The purpose of this experiment is to make a suitable animal model of disc displacement without reduction for studying and understanding the cellular and morphologic events in posterior attachment of TMJ including early changes which were difficult to be observed in human TMJs. The results of this investigation suggest the following conclusions : 1. Authors induced anterior disc displacement surgically in rabbits with new method to examine histologic changes of posterior attachment. Tissue reactions of this model seem to be similar to those observed in human disc displacement. We think this animal model for anterior disc displacement may be used to explore and evaluate objectively the effects of many treatment modalities in disc displacements. 2. The animal disease model showed inflammation at early stage(one and ten days). At this stage there were mild-to-severe mononuclear inflammatory cell infiltration, numerous newly formed vessels, vessel dilatation and engormement and many fibroblasts. 3. At middle stage(three weeks), fibrosis occurred, where fibroblasts decreased in number, but their cytoplasm was profuse indicating high activity. Collagen fibers increased in number and the tissue looked more dense. 4. At late stage(five weeks and eight weeks) showed degenerative changes including perforation of posterior attachment, disintegration of collagen fiber bundles, degeneration of fibroblasts, metastatic ossification, and dystrophic calcification.

• 대한치과교정학회지
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• 제30권3호
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• pp.297-306
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• 2000

최대 개구운동시 하악골의 개구능력과 과두의 활주 운동 양상을 관찰하고 두개안면골격 형태요소와의 상관성을 알아보기 위해 경희의료원 교정과에 내원한 10세 전후의 아동 68명의 최대감합위 및 최대개구위 측모두부방사선사진을 분석하고 통계 처리하여 다음과 같은 결론을 얻었다. 1. 최대개구량의 평균치는 47.1mm, 최대개구운동시 과두의 이동 직선거리는 18.1mm, 과두의 수평 이동거리는 17.5mm, 수직이동거리는 3.8mm였으며 이동경사도는 $13.1^{\circ}$이었다. 2. 전방두개저의 길이, 하악골 및 상악복합체의 전후방적인 길이가 길수록, 하악상행지가 전방으로 경사할수록, 후안면 고경이 크고 하악의 경사도가 작을수록 개구량이 컸다. 3. 하악상행지가 직립된 경사도를 가질수록, 하악골의 만곡도가 클수록 과두는 수직적인 활주운동을 하였다. 4. 상악복합체가 전후방적으로 길수록 과두는 전방으로 길게 활주운동을 하였다. 이상의 내용으로 볼 때 최대개구위 측모두부방사선사진은 부정교합 환자의 개구운동시 하악골의 운동양상을 평가할 수 있는 진단 자료로서 유용하며 하악골의 개구운동 양상은 두개안면골격의 특정한 형태적 요소와 연관성을 가지며 악관절의 기능적 해부 형태에 관한 정보를 제공하는 것으로 사료된다.

• 대한지리학회지
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• 제47권2호
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• pp.159-178
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• 2012

백두산의 현재 지형은 반복적인 화산활동과 함께 신생대 제4기 기후변화로 추동된 빙하 활동의 결과물이다. 그러나 여전히 백두산 빙하지형의 존재에 대한 의문들은 해소되지 못해왔다. 이에, 본 연구에서는 야외조사를 통하여, 빙하지형에 관련된 지형들을 제시하고, 이의 연대측정을 실시하였으며, 제4기 화산 활동과의 관련성을 살펴보았다. 가속기 질량 분석기를 이용하여 빙하의 후퇴 이후 지표면에 노출되었을 백두산 천지 서사면의 빙하 기반암 지형 면에 대하여, 우주선 유발 동위원소 $^{36}Cl$의 농도를 측정하여 46~26ka의 표면노출연대를 얻었으며, 이는 적어도 천지 서쪽 사면은 지난 최종빙기 이후의 화산활동에 영향을 받지 않은 것을 지시하며, 특히 대략 천 년 전에 있었다고 보고되는 폭발성 분화에도 영향을 거의 받지 않았을 가능성이 매우 높다. 또한, 화산 쇄설물의 비대칭적 퇴적을 고려했을 때, 계절적으로 이 분화는 북서풍이 탁월한 겨울철에 발생한 것으로 사료된다. 빙하지형과 이의 연대 결과를 바탕으로 지형 발달과정을 복원해보면, 백두산의 빙하 지형은 지난 빙기까지 곡빙하(valley glacier)들이 연합한 빙모(ice cap) 형태에서 고립된 곡빙하를 거쳐 화산체 내부의 소규모 권곡(cirque) 형태로 변화한 것으로 추론된다.