• Title/Summary/Keyword: Morphologic

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Validation of the optimal scaffold pore size of nasal implants using the 3-dimensional culture technique

  • Nam, Jeoung Hyun;Lee, So Yun;Khan, Galina;Park, Eun Soo
    • Archives of Plastic Surgery
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    • v.47 no.4
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    • pp.310-316
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    • 2020
  • Background To produce patient-specific nasal implants, it is necessary to harvest and grow autologous cartilage. It is crucial to the proliferation and growth of these cells for scaffolds similar to the extracellular matrix to be prepared. The pore size of the scaffold is critical to cell growth and interaction. Thus, the goal of this study was to determine the optimal pore size for the growth of chondrocytes and fibroblasts. Methods Porous disc-shaped scaffolds with 100-, 200-, 300-, and 400-㎛ pores were produced using polycaprolactone (PCL). Chondrocytes and fibroblasts were cultured after seeding the scaffolds with these cells, and morphologic evaluation was performed on days 2, 14, 28, and 56 after cell seeding. On each of those days, the number of viable cells was evaluated quantitatively using an MTT assay. Results The number of cells had moderately increased by day 28. This increase was noteworthy for the 300- and 400-㎛ pore sizes for fibroblasts; otherwise, no remarkable difference was observed at any size except the 100-㎛ pore size for chondrocytes. By day 56, the number of cells was observed to increase with pore size, and the number of chondrocytes had markedly increased at the 400-㎛ pore size. The findings of the morphologic evaluation were consistent with those of the quantitative evaluation. Conclusions Experiments using disc-type PCL scaffolds showed (via both morphologic and quantitative analysis) that chondrocytes and fibroblasts proliferated most extensively at the 400-㎛ pore size in 56 days of culture.

Morphologic descritions of Taenia asiatica sp. n. (신종 조충 Taenia asiatica sp.n.의 형태학적 기재)

  • 엄기선;임한종
    • Parasites, Hosts and Diseases
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    • v.31 no.1
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    • pp.1-6
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    • 1993
  • Among taenited tapeworms inferting humans though pork or beef, Taenia solium Linnaeus 1758 and Taenia sagina Goeze 1782 have already been. Based on the morphologic characteristics of adult and metacestodes of Asian Taenia saginata, the third kind of human taeniid tapeworm konwn to disdute in Asian counteries, a new spscies name of Taenia asiatica is proposed. In addition to the known biology in their intermediate hosts, T.asiatica was diffierent morphologically from Taenia saginata Goeze 1782 in having the unarmed rostellum on the scolex of adlut, the large number of 'unterinetwigs' and the existence of 'posterior protuberance'. These structures in the gravid proglottids were used as taxonmic keys in taeniid tapeworms for the first time. T. asiatica metacestode (Cysticercus viscerotropica)was different morphologically from T. saginata metacestode (Cysticercus bovis) in having wartlike formations on external surface of the bladder wall.

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Cytologic Evaluation of Adenocarcinoma and its Mimics in Sputum and Bronchial Washings (객담과 기관지세척액에서 폐 샘암종과 유사한 병변의 세포소견 분석)

  • Park, In-Suh;Choi, Suk-Jin;Kim, Lucia;Han, Jee-Young;Kim, Joon-Mee;Park, Jung-Ae;Chu, Young-Chae
    • The Korean Journal of Cytopathology
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    • v.18 no.1
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    • pp.36-45
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    • 2007
  • Objective : To identify key cytologic features for diagnosis of adenocarcinoma and morphologic differentiation from reactive/reparative respiratory epithelium. Study Design : The cytomorphologic features of 145 pulmonary cytology specimens (sputum and bronchial washing), which included 117 histologically proven adenocarcinomas and 28 non-neoplastic lesions, cytologically diagnosed as atypia and suspicious for malignancy (adenocarcinoma) were reviewed retrospectively. We analyzed 11 morphologic criteria in pulmonary cytologic specimens. Results: Over 110 of 117 cases of adenocarcinomas revealed nuclear membrane irregularities, non-cohesive cells, single atypical cells, moderate to markedly enlarged nuclei and an increased nuclear/cytoplasmic (N/C) ratio. Chromatin clearing, chromatin heterogeneity and hyperchromasia were seen in 102, 99 and 97 cases, respectively. All 28 cases involving non-neoplastic lesions revealed hyperplastic reactive pneumocytes, hyperplastic reactive bronchial epithelium, or degenerating macrophages. The non-neoplastic lesions revealed a small number of atypical cell clusters and paucity or absence of atypical single cells. Conclusion : The most important morphologic features for diagnosis of adenocarcinoma are nuclear membrane irregularities, non-cohesive cells, single atypical cell, moderate to markedly enlarged nuclei and increased N/C ratio. Chromatin clearing, chromatin heterogeneity and hyperchromasia were also helpful features, while pleomorphism and prominent nucleoli were less valuable.

Physical and Morphological Characteristics Change of Hair according to Water Content when Heat Permanent Wave is treated (열펌 시술시 수분함량에 따른 모발의 물리적·형태학적 특성 변화)

  • Lee, Soon-Hee;Kim, Sung-Nam
    • Fashion & Textile Research Journal
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    • v.10 no.3
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    • pp.389-393
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    • 2008
  • The purpose of this study is to provide beauticians with the fundamental material for them to use effectively heat permanent wave and satisfy their customers. It carried out an experiment with hair of a woman in her late twenties to investigate the change of physical and morphologic characteristics by its water content when performing heat permanent wave. It evaluated the water content as 0g, 1g, 2g, 3g and 4g respectively when performing the heat permanent wave on hair, then it compared and observed the wave type, tensile strength and elongation for its physical change also observed the morphologic change by scanning electronic microscope and transmission electronic microscope. The result of experiment on the physical specificity revealed that the wave was the most ideal when the water content was 2 g. The material with much water content made wave but the result was not satisfied. In the case of hair with water content of nearly 0g didn't make wave. In terms of tensile strength and elongation, the tensile strength was generally reduced as hair was damaged, on the contrary, the elongation was increased. It observed the change of morphologic characteristic and got the result that the damage on hair cuticle was deepen as its water content was decreased. It also showed the result that damage happened on hair cuticle more than hair cortex with the observation of fine structure on hair section by transmission electronic microscope. Generally chemical treatment damaged hair. Under consideration of this aspect, the ultimate goal of this thesis is to minimize the damage of hair caused by chemical treatment and get the satisfaction on the hair style. The result of experiment presented that the hair showed the best result when its water content was 2 g.

Differentiation between Glioblastoma and Solitary Metastasis: Morphologic Assessment by Conventional Brain MR Imaging and Diffusion-Weighted Imaging

  • Jung, Bo Young;Lee, Eun Ja;Bae, Jong Myon;Choi, Young Jae;Lee, Eun Kyoung;Kim, Dae Bong
    • Investigative Magnetic Resonance Imaging
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    • v.25 no.1
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    • pp.23-34
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    • 2021
  • Purpose: Differentiating between glioblastoma and solitary metastasis is very important for the planning of further workup and treatment. We assessed the ability of various morphological parameters using conventional MRI and diffusion-based techniques to distinguish between glioblastomas and solitary metastases in tumoral and peritumoral regions. Materials and Methods: We included 38 patients with solitary brain tumors (21 glioblastomas, 17 solitary metastases). To find out if there were differences in the morphologic parameters of enhancing tumors, we analyzed their shape, margins, and enhancement patterns on postcontrast T1-weighted images. During analyses of peritumoral regions, we assessed the extent of peritumoral non-enhancing lesion on T2- and postcontrast T1-weighted images. We also aimed to detect peritumoral neoplastic cell infiltration by visual assessment of T2-weighted and diffusion-based images, including DWI, ADC maps, and exponential DWI, and evaluated which sequence depicted peritumoral neoplastic cell infiltration most clearly. Results: The shapes, margins, and enhancement patterns of tumors all significantly differentiated glioblastomas from metastases. Glioblastomas had an irregular shape, ill-defined margins, and a heterogeneous enhancement pattern; on the other hand, metastases had an ovoid or round shape, well-defined margins, and homogeneous enhancement. Metastases had significantly more extensive peritumoral T2 high signal intensity than glioblastomas had. In visual assessment of peritumoral neoplastic cell infiltration using T2-weighted and diffusion-based images, all sequences differed significantly between the two groups. Exponential DWI had the highest sensitivity for the diagnosis of both glioblastoma (100%) and metastasis (70.6%). A combination of exponential DWI and ADC maps was optimal for the depiction of peritumoral neoplastic cell infiltration in glioblastoma. Conclusion: In the differentiation of glioblastoma from solitary metastatic lesions, visual morphologic assessment of tumoral and peritumoral regions using conventional MRI and diffusion-based techniques can also offer diagnostic information.

TAURODONTISM

  • Kim, Dong-Joon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.5 no.1
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    • pp.44-46
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    • 1978
  • This is to report of a case of bilateral taurodontism of mandibular right & left 1st deciduous molars of 4 year-old female. Taurodontism is a morphologic condition of tooth, it seems to be caused by delayed or incomplete union of horizontal epithelial flaps of the epithelial diaphragm. This union is necessary in order to initiate dentinogenesis in the bifurcation area.

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