• Asian Pacific Journal of Cancer Prevention
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• 제17권2호
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• pp.491-495
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• 2016

Background: Small B-cell non-Hodgkins lymphoma (NHL) is difficult to be distinguished from non-neoplastic reactive processes using conventional haematoxylin-eosin (HE) staining due to different interpretations among pathologists with diagnosis based on morphologic features. Ancillary examinations such as immunohistochemical (IHC) staining are essential. However, negative or doubtful results are still sometimes obtained due to unsatisfactory tissue processing or IHC technique. The polymerase chain reaction (PCR) as a molecular diagnostic technique is very sensitive and specific. Clonality detection of heavy chain immunoglobulin (IgH) gene rearrangement has been widely used to establish diagnosis of B-cell NHL. Aims: To elaborate interobserver variation in small B-cell NHL diagnosis based on morphologic features only and to confirm sensitivity and specificity of the PCR technique as an ancillary method. Materials and Methods: A toptal of 28 samples of small B cell NHL and suspicious lymphoma were interpreted by 3 pathologists in Sardjito General Hospital based on their morphology only. The reliability of assessment and the coefficient of interobserver agreement were calculated by Fleiss kappa statistics. Interpretation results were confirmed with IHC staining (CD20, CD3, Bcl2). PCR was performed to analyze the clonality of IgH gene rearrangement. Results: Interobserver agreement in morphologic evalution of small B cell NHL and chronic lymphadenitis revealed kappa coefficient 0.69 included in the substantial agreement category. The cases were divided into 3 groups based on morphology and IHC results; lymphoma, reactive process and undetermined group. PCR analysis showed 90% sensitivity and 60% specificity. Conclusions: The present study revealed a substantial agreement among pathologists in small B-cell NHL diagnosis. For difficult cases, PCR is useful as complementary method to morphologic and IHC examinations to establish definitive diagnosis.

• 대한치과보철학회지
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• 제38권2호
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• pp.178-190
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• 2000

The purpose of this study was to evaluate the surface roughness of Ni-Cr-Be alloy($Verabond^{(R)}$, Aalba Dent Inc., USA) according to electrolyte concentration and etching time. Total of 150 metal specimens ($12{\times}10{\times}1.5mm$) composed of 5 polisded specimens, 5 sandblasted specimens, 140 etched specimens were prepared. Etched groups were divided into 28 groups by the $HClO_4$ concentrations(10, 30, 50, 70%) and etching times(15, 30, 60, 120, 180, 240, 300 seconds). The mean surface roughness(Ra) and the etching depth were measured with Optical 3-dimensional surface roughness measuring machine(Accura 1500M, Intek Engineering Co., Korea) and observed under SEM. The results obtaind were as follows: 1. Surface roughness(Ra) and etching depth were affected by the order of etching time, electrolyte concentration, and their interaction(P<0.05). 2. Surface roughness(Ra) and etching depth were increased with etching time in 10%, 30% electrolyte concentrations, but they had no significant difference with etching time in 70% (P<0.05). 3. Surface roughness(Ra) and etching depth decreased in the order of 30, 10, 50, 70% electrolyte concentrations from 120 seconds etching time(P<0.05). 4. The remarkable morphologic changes in etched surface were observed along the grain boundaries in 15, 30 seconds of 10%, 30% concentrations and the morphologic changes could be denoted in the grains themselves as well as along the boundaries with the lapse of time. Even though the noticeable morphologic changes also took place in etched surface with 50% concentration, the degree of changes were less than that of changes with 10%, 30%. However, there were little morphologic changes with 70% concentration regardless of etching time. 5. Surface roughness(Ra) of sandblasting group with $50{\mu}m\;Al_2O_3$ had no significant difference with 30%-30 seconds etched group(P<0.05).

• 대한한의학회지
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• 제25권3호
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• pp.160-168
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• 2004

Background and Objectives: Tinnitus is on the increase due to the increase in the elderly population, industrial pollution and noise pollution. This symptom is especially marked in patients with a hearing problem and the relationship between cause, mechanism and treatment is poorly understood. The characteristics of tinnitus and other hearing problems are well brought out using an animal model with salicylate ototoxicity. Therapeutic effects of Uncariae Ramulis and Testudinis Plastrum were expected in tinnitus and hearing problems; therefore we experimented on an animal model with salicylate ototoxicity. Salicylate is one of the most commonly prescribed drugs, although it has been recognized that salicylate induces hearing loss and tinnitus reversibly. The purpose of this study was to find the therapeutic effects of this by the morphologic study using salicylate ototoxicity. Materials and Methods : Twelve healthy Sprague-Dawley rats were divided into three groups: normal, control and sample. The sample group was treated with the extract of Uncariae Ramulis and Testudinis Plastrum (1cc/100g, once a day for 6 days). Then, to induce the salicylate ototoxicity in the control and sample groups, rats were injected intraperitoneally with sodium salicylate (500mg/kg). We observed the morphologic changes in the cochlea of the rats every 2, 3, 4 and 5 hours after injection. Results : The outer hair cells showed marked changes. Vacuolization formed in the cuticular plate and the endoplasm of the control group. The endoplasm and the cuticular plates of the sample group after 2 hours were similar to the control group, but the cuticular plates of the sample group observed after 3, 4 and 5 hours were not similar. Conclusions : The results suggest that an extract of Uncariae Ramulis and Testudinis Plastrum has therapeutic effects on an animal model with salicylate ototoxicity.

• 사상체질의학회지
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• 제11권2호
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• pp.195-207
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• 1999

1. 연구배경 및 목적 외형적 특정을 통한 사상체질진단의 목적으로 특히, 임상에서 쉽게 접할 수 있는 수족(手足)의 체질별 형태학적인 차이를 찾고자 하였다. 2. 방법 설문지와 임상적 진단을 거쳐 체질이 최종적으로 분류된 148명을 대상으로 하였으며. Martin식 계측법을 준용하여 조사 대상자들의 수족(手足)을 생체 계측을 하였다. 3. 결과 신체의 특정으로는 태음인이 타 체질에 비해 몸무게가 무거웠다. 손의 특징으로는 손목둘레, 손 전체에서 손가락이 차지하는 비, 손목너비, 손바닥길이, 손최대너비, 손목너비, 두 번째, 세 번째와 네 번째 손가락 길이에서 체질별 유의성이 관찰되었다. 발의 특징으로는 발두께, 발너비, 발목둘레에서 체질별 유의성이 관찰되었다.

• The Korean Journal of Pain
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• 제36권2호
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• pp.208-215
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• 2023

Background: To evaluate the feasibility, inter-reader reliability, and intra-reader reliability for various morphological features reported to be related to iliotibial band friction syndrome (ITBFS) on knee magnetic resonance imaging (MRI). Methods: A total of 145 patients with a clinical diagnosis and knee MRI findings consistent with ITBFS were included in the "study group" and 232 patients without knee pathology on both physical examination and MRI were included in the "control group". Various morphologic features on knee MRI were assessed including the patella shape, patella height, lateral epicondyle anterior-posterior (AP) width, lateral epicondyle height, ITB diameter (ITB-d), and ITB area (ITB-a). Results: Patients in the study group had significantly higher lateral epicondyle height (13.9 mm vs. 12.92 mm, P = 0.003), ITB-d (2.9 mm vs. 2.0 mm, P = 0.022), and ITB-a (38.5 mm² vs. 23.8 mm², P < 0.001) than the control group. ITB-a showed higher area under the curve index (0.849 with 74.1% sensitivity and 72.4% specificity at a 30.3 mm² cutoff) than ITB-d (0.710 with 70.8% sensitivity and 61.2% specificity at 2.4 mm cutoff) and lateral epicondyle height (0.776 with 72.4% sensitivity and 67.8% specificity at 13.4 mm cutoff). However, only the inter-reader agreement for ITB-a (intraclass correlation coefficient = 0.65) was moderate, while the agreements for other morphologic features were good or excellent. Conclusions: Lateral epicondyle height seems to be a reliable and feasible morphologic feature for diagnosis of ITBFS.

• Journal of information and communication convergence engineering
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• 제5권2호
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• pp.126-130
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• 2007

This paper proposes a method that automatically extracts characteristics of cracks such as length, thickness and direction, etc., from a concrete surface image with image processing techniques. This paper, first, uses the closing morphologic operation to adjust the effect of light extending over the whole concrete surface image. After applying the high-pass filtering operation to sharpen boundaries of cracks, we classify intensity values of the image into 8 groups and remove intensity values belong to the highest frequency group among them for the removal of background. Then, we binarize the preprocessed image. The auxiliary lines used to measure cracks of concrete surface are removed from the binarized image with position information extracted by the histogram operation. Then, cracks broken by the removal of background are extended to reconstruct an original crack with the $5{\times}5$ masking operation. We remove unnecessary information by applying three types of noise removal operations successively and extracts areas of cracks from the binarized image. At last, the opening morphologic operation is applied to compensate extracted cracks and characteristics of cracks are measured on the compensated ones. Experiments using real images of concrete surface showed that the proposed method extracts cracks well and precisely measures characteristics of cracks.

• Imaging Science in Dentistry
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• 제31권4호
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• pp.235-240
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• 2001

Purpose: To evaluate displacement and morphologic changes of the temporomandibular joint (TMJ) disc in patient with internal derangement using magnetic resonance imaging (MRI). Materials and Methods: One hundred and forty five MR images of TMJs in 73 patients were evaluated. Positional and morphologic changes of the TMJ discs were assessed. Lateral or medial disc displacement was also evaluated on coronal images. Results: Among 63 discs with anterior disc displacement, 37 discs were assessed as a biconcave disc and 21 as a deformed disc. Rotational disc displacement was observed in 35 discs. Anteromedial disc displacement was observed in 29 discs, and anterolateral direction in 6 discs. Among 35 rotational displacement, 5 biconcave discs and 21 deformed discs were observed. Conclusion : Rotational and sideways displacement of TMJ discs were found to be common and an important aspect of internal derangement. This study also suggests that sagittal and coronal images of the TMJ have complementary abilities for an assessment of joint abnormality.

• Imaging Science in Dentistry
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• 제35권3호
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• pp.141-146
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• 2005

Purpose : The purpose of this study was to investigate the preliminary use of morphologic operation (MO) in analyzing trabecular pattern of alveolar bone for the predicting systemic osteoporosis. Materials and Methods : Study subjects consisted of 35 females (average age 48.5 years) and 25 males (average age 25.8 years). Bone mineral density BMD $(grams/cm^2)$ of lumbar spine and proximal femur of these subjects were measured by a dual energy X-ray absorptiometry (DEXA). Regions of interest (ROIs) were selected from the digitized periapical radiographs of subjects' posterior jaw. A custom computer program processed morphology operations of ROIs. We compared mean values of 11 MO variables according to the osteoporotic group divided by the T-scores of DEXA. We also studied correlation between radiographic density and these MO variables. Results : The mean radiographic densities insignificantly correlated with MO variables. There were statistically significant differences among the values of 9 MO variables according to the osteoporotic group. Conclusion Morphologic operation can be effective in analyzing trabecular pattern of alveolar bone for the predicting osteoporosis.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.247-249
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• 2006

The objective of this study is to detect a inter-tidal topographic change in a decade. Waterline extraction is a one of widely used method to generate digital elevation model (DEM) of tidal flat using multi-temporal optical data. This method has been well known that it is possible to construct detailed topographic relief of tidal flat using waterlines In this study, we generated two sets of tidal flat DEM for the southern Ganghwado. The DEMs showed that the Yeongjongdo northern tidal flat is relatively high elevation with steep gradients. The Ganghwado southern tidal flat is relatively low elevation and gentle gradients. To detect the morphologic change of tidal flat during a decade, we compared between early 1990's DEM and early 2000's DEM. Erosion during a decade is dominant at the west of southern Ganghwado tidal flat, while sedimentation is dominant at the wide channel between the southern Ganghwado and Yeongjongdo tidal flats. This area has been commonly affected by high current and sedimentation energy. Although we are not able to verify the accuracy of the changes in topography and absolute volume of sediments, this result shows that DEM using waterline extraction method is an effective tool for long term topographic change estimation.

• Journal of Chest Surgery
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• 제23권6호
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• pp.1074-1083
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• 1990

This study was evaluated the metabolic, physiologic and histologic effects of myocardial protection of verapamil[isoveratril]on isolated rat hearts to 90 minutes of ischemic arrest. Heart was perfused with a modified Kreb’s Henseleit bicarbonate buffer with glucose and arrested with retrograde coronary perfusion by glucose insulin[GI], potassium and verapamil. Mean aortic systolic pressure, heart rate, coronary flows were measured and morphologic changes were examined during working heart perfusion. Perfusion and arrest were controlled four groups subjected 60 isolated rat hearts. Four groups hearts reperfused during 40 minutes after 90 minutes global ischemia for physiologic recovery. 15 hearts of four groups were assayed to histological morphologic changes. GI treated hearts recovered less than 28% of function and changed more than 80% of mitochondria of control group. Verapamil hearts[0.2, 0.1 gm/kg] recovered more than 88% of function and permitted the maintenance of continuous cellular level of Serum Glutamic Oxalaxetate Transaminase[SGOT], but declined 28% of Phosphate Kinase[CP], GI treated heart showed widespread evidence of extensive damage of mitochondria. The damage was that interstitial huge edema are present and there was contraction band formation within the swollen cells. The verapamil and potassium group were not found morphologic change compared with control group. Their functions were shown that metabolic and physiologic action of verapamil-group lasted 20 minutes longer than potassium group.