• 대한핵의학회지
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• 제36권3호
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• pp.203-208
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• 2002

만성간질환 환자에서 호흡곤란을 초래하는 간폐 증후군 발생한다는 사실은 잘 알려져 있다. 단락을 입증하기 위해서는 대비증강심초음파, 폐혈관조영술, $^{99m}Tc-MAA$ 폐관류스캔 등을 이용할 수 있다. 이 중에서도 $^{99m}Tc-MAA$를 이용한 폐관류스캔은 매우 안전하고 간편하게 단락의 존재 여부를 확인할 수 있으며, 단락의 양을 계산할 수도 있다. 정량이 가능하므로 병의 경과를 추적하는 지표로서도 유용할 것이다. 저자들은 $^{99m}Tc-MAA$ 폐관류스캔으로 진단한 간폐증후군의 증례를 문헌고찰과 함께 보고하였다.

• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.2037-2043
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• 2017

The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitates viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we describe a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscopy (FE-SEM) showed that the immobilized aptamer bound specifically to the H1 subtype but not to the H5 subtype. Assessment by cyclic voltammetry (CV) also demonstrated that the immobilized aptamer on the indium thin oxide-coated surface was specifically bound to the H1 subtype only, which was consistent with the ELISA and FE-SEM results. Further measurement of CV using various amounts of H1 subtype provided the detection limit of the immobilized aptamer, which showed that a nanomolar scale of target protein was sufficient to produce the signal. These results indicated that the selected aptamer can be an effective probe for distinguishing the subtypes of influenza viruses by monitoring current changes.

• 기술혁신학회지
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• 제16권2호
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• pp.408-423
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• 2013

창조와 혁신이 성공의 중요한 키워드로 대두되면서 창의적 문제해결 방법론인 트리즈(TRIZ)에 대한 관심이 고조되고 있다. 지금까지 트리즈는 전자 및 기계분야에 도입되어 제품혁신의 원동력으로 활용되고 있어, 본 연구에서는 미래유망기술인 바이오분야에 트리즈 기법을 적용하여 문제를 해결하고 혁신적인 연구개발을 추진할 수 있는지의 가능성을 타진해보고자 하였다. 바이오분야 연구 중 홍삼제조과정에서 발생하는 문제를 선정하고, 이의 문제와 문제원인 구분 및 모순을 도출하고 트리즈의 발명 40가지 원리를 적용하여 홍삼 제조과정에서 발생되는 갈라짐 문제를 해결하고자 하였다. 홍삼은 수삼을 스팀 등의 방법으로 쪄서 익혀 말린 담갈색의 인삼으로, 홍삼의 갈라짐은 유효성분의 유출과 외형등급 하락으로 상품성을 떨어트리는 주요 원인이 된다. 트리즈 툴(Tool) 중 모순 매트릭스 및 브레인스토밍을 통해 적용 가능한 발명원리를 도출하고, 실험을 통해 홍삼 제조과정에서 갈라짐을 방지할 수 있는 유용한 방법들을 제안하였다.

• 품질경영학회지
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• 제46권1호
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• pp.1-10
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• 2018

Purpose: This paper provides an idea on the future prospect for change in steps of the six sigma DMAIC project under the environment of the 4th industrial revolution. Methods: First, the purpose and activities required in each step of DMAIC are reviewed. Next, activities are reviewed together with tools and techniques, considering the purpose and the environmental changes of the 4th Industrial Revolution. Finally, the best approaches for achieving the purpose are prospected to get an idea on future change. Results: The purpose of each phase of DMAIC is expected to remain unchanged. But activities, techniques, or methods will be replaced with more effective and efficient ones. Also, many activities may possibly be executed by a system instead of people like BB, GB or team members. Moreover, DMAIC may not be a project any more but a routine job of the system in the future. Conclusion: Under the environment of the 4th industrial revolution, many activities including analyzing various types of data and extracting valuable information, will be executed by a system with proper algorithms instead of people. And six sigma improvement projects may be intrinsic parts of the system and may not exist as separate projects any more.

• Korean Chemical Engineering Research
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• 제52권1호
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• pp.106-112
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• 2014

본 연구에서는 미세액적을 효과적으로 합류시키기 위하여 미세기둥 구조들에 따른 영향 및 정체 현상 조절에 관한 연구를 수행하였다. 최적의 미세액적 합류조건을 찾기 위하여 매우 정교하게 조절이 가능한 미세 밸브를 접목하였다. 수용성 미세액적은 연속상으로 0.5 wt% Span 80이 함유된 미네랄 오일을 사용하였다. 합류과정은 미세유체 칩 내부에 위치한 미세기둥의 구조와 배치를 통해 액적 주위의 압력과 액적 내부의 라플라스 압력의 차이를 조정하여 수행된다. 마지막으로 최적의 합류 구조를 지닌 미세유체시스템에서 효소 생화학반응 실험을 수행함으로써 본 장치가 생물학 및 생화학 실험을 수행하는 유용한 도구로서 사용될 수 있음을 입증하였다.

• 대한임상검사과학회지
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• 제42권3호
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• pp.116-124
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• 2010

This study was aimed to clerify the cytotoxicity of some plant extracts such as Hosta longissima HONDA (HL), Hemerocallis fulva var. Kwanso REGL (HFVK), Hemerocallis fulva L (HF), Macrocapium officinale NAKAI (MO) and Mentha canadensis var. piperascens HARA (MCVP), the cultured NIH3T3 fibroblasts were treated with 25, 50, 100, 150 and $200{\mu}g/mL$ of five kinds of plant extracts for 48 hours, respectively. The cytotoxicity of plant extracts was measured by MTT and NR assays for the cell viability, and XTT assay for the cell adhesion activity. In this study, HL, MO and FHVK extracts showed the range of midtoxic-non toxic by the criteria of chemical cytotoxicity. While, the HF and MCVP extracts showed midtoxic. In the extract cytotoxicity, HL, MO and FHVK extracts showed non-toxic by the criteria of extract cytotoxicity. While, HF extract was determined as lower-toxic. In the responsive sensitivity of each plant extract on colorimetric assays, HF extract was sensitive to mitochondrial enzyme by MTT assay, lysosomal enzyme by NR assay and mitochondrial nucleus by XTT assay. While, MCVP extract was sensitive to mitochondrial enzyme by MTT assay and lysosomal enzyme by NR assay than other assays. While, HL, HFVK and MO extracts were most sensitive to NR assay. Cell culture is one of useful materials in the screening of cytotoxic and recovary effect on the putative chemical agents or plant extract. And also, colorimetric assay is regarded as very useful tools for quantitative measurement of cytotoxic effect on plant extracts in vitro.

• 융합보안논문지
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• 제17권2호
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• pp.3-13
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• 2017

지식정보화 사회가 도래하면서 많은 국내기업이 핵심기술 및 지식재산 확보를 위해 기술개발에 많은 투자를 하고 있다. 하지만 적극적인 기술개발 투자에 비해 기업이 보유한 기술을 보호하기 위한 보안투자가 미흡한 결과 다수의 기업 및 연구소에서 기술 유출 사건이 급격하게 증가하고 있다. 이러한 기술유출의 증가는 단순히 기업에 피해뿐만 아니라 국가 경제에도 직 간접적인 악영향을 미치고 있는 실정이다. 산업기술유출은 주로 전 현직 직원에 의해 이뤄지고 있지만, 이를 중점적으로 비교 분석한 연구는 많지 않다. 따라서 본 연구는 2014년부터 2016년 동안 발생한 산업기술유출 사례를 기술유출 피해기업의 유형, 회사 내 기술유출 주체자의 직위, 기술유출의 공범여부, 기술유출에 사용된 도구, 기술유출의 동기로 유형화하여 산업기술유출 실태를 파악하였다. 이러한 유형별 분석을 통해 산업기술유출의 패턴과 특징에 대해 조사하고 산업기술유출 방지를 위한 산업보안 증진방안을 모색하였다.

• 기술혁신연구
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• 제12권3호
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• pp.1-24
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• 2004

E-Learning is another way of teaching and learning. E-learning is a networked phenomenon allowing for instant revisions and distribution, and goes beyond training and instruction to the delivery of information and tools to improve performance. The benefits of e-learning are many, including cost-effectiveness, enhanced responsiveness to change, consistency, timely content, flexible accessibility, and providing customer value. The proponents of e-learning stress the importance of using communities of interest to support and enhance the learning process. They also emphasizes that people learn more effectively when they interact and are involved with other people participating in similar endeavors. Although the role of e-learning in higher education has significantly increased, the resistance to new technology by professors and lecturers in university and colleges worldwide remains high. The purpose of this study is to identify the determinants of attitude and planned behavior toward e-learning class in universities. A survey methodology was used to investigate a proposed model of influence, and structural equation modeling was used to analyze the results. The hypothesized model was largely supported by this analysis, and the overall results indicate that attitude toward e-learning systems is mostly influenced by the perceived ease of use as well as the level of perceived usefulness, where both factors are influenced by years of experiences in using cyber system and the technical support level. As in other TAM related research, it can be concluded that the perceived ease of use and perceived usefulness contribute to the future use of e-learning system.

• 대한한방부인과학회지
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• 제27권1호
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• pp.185-192
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• 2014

Purpose: The purpose of this study is to report the effect on cold hypersensitivity of hands, feet and low abdomen by Sasang constitutional treatment. For this purpose, the effects of a treatment were measured quantitatively using Digital Infrared Thermographic Imaging (DITI). Methods: It was a case of report, the patient was a 25-year-old female whose chief complaint was cold hypersensitivity of hands and feet and low abdomem. We treated the patient with Sasang constitutional treatment, and the effect of a treatment was evaluated by DITI. Results: After Sasang constitutional treatment, the differences of temperature of hands, feet and low abdomem were decreased on DITI screening, respectively. Conclusions: Sasang constitutional treatment on cold hypersensitivity of hands, feet and low abdomen is effective. Further studies on diagnosis tools of low abdomen cold hypersensitivity are needed.

• Toxicological Research
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• 제35권1호
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• pp.83-91
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• 2019

Nanoparticles (NPs) have been recognized as both useful tools and potentially toxic materials in various industrial and medicinal fields. Previously, we found that zinc oxide (ZnO) NPs that are neurotoxic to human dopaminergic neuroblastoma SH-SY5Y cells are mediated by lipoxygenase (LOX), not cyclooxygenase-2 (COX-2). Here, we examined whether human bone marrow-derived mesenchymal stem cells (MSCs), which are different from neuroblastoma cells, might exhibit COX-2- and/or LOX-dependent cytotoxicity of ZnO NPs. Additionally, changes in annexin V expression, caspase-3/7 activity, and mitochondrial membrane potential (MMP) induced by ZnO NPs and ZnO were compared at 12 hr and 24 hr after exposure using flow cytometry. Cytotoxicity was measured based on lactate dehydrogenase activity and confirmed by trypan blue staining. Rescue studies were executed using zinc or iron chelators. ZnO NPs and ZnO showed similar dose-dependent and significant cytotoxic effects at concentrations ${\geq}15{\mu}g/mL$, in accordance with annexin V expression, caspase-3/7 activity, and MMP results. Human MSCs exhibited both COX-2 and LOX-mediated cytotoxicity after exposure to ZnO NPs, which was different from human neuroblastoma cells. Zinc and iron chelators significantly attenuated ZnO NPs-induced toxicity. Conclusively, these results suggest that ZnO NPs exhibit both COX-2- and LOX-mediated apoptosis by the participation of mitochondrial dysfunction in human MSC cultures.