• Title/Summary/Keyword: Monokaryotic strains

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Breeding of a new cultivar 'Dadam' for Lentinula edodes sawdust cultivation (표고 톱밥재배용 신품종 '다담' 육성)

  • Kim, Jeong-han;Shin, Bok-Eum;Baek, Il-Sun;Choi, Jong-In;Ha, Tai-Moon;Jung, Gu-Hyun
    • Journal of Mushroom
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    • v.19 no.2
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    • pp.96-102
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    • 2021
  • A new cultivar of oak mushroom 'Dadam' was bred from monokaryotic strains of 'GMLE36062-4' and 'GMLE36288-34'. The optimum temperature for mycelial growth of the new cultivar 'Dadam' on potato dextrose agar was 19-22℃. Total cultivation period of the new cultivar, from innoculation to its first harvest, was 135-139 days, similar to that of the control cultivar 'Hwadam'. The pileus color and stipe thickness of the new cultivar were darker and thinner than those of 'Hwadam'. Total yield of 'Dadam' was 621 g per 3 kg substrate, and is higher than that of 'Hwadam' (371 g). In farmhouse field test, it showed that the period of mycelial growth, browning, and fruiting body formation were the same as those of the control cultivar L808. The number of available fruiting bodies of 'Dadam' was 15, and is lower than that of the control (47), therefore, it was possible to save workforce in thinning. The total yield for 2 flush was 480 g for 'Dadam', similar to the 473 g of the control cultivar.

Breeding of a new cultivar of White Flammulina velutipes, 'Seolhan' (흰색 팽이 신품종 '설한' 육성)

  • Im, Ji-Hoon;Oh, Minji;Oh, Youn-Lee;Kim, Minseek;Lee, Yun-Sang
    • Journal of Mushroom
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    • v.19 no.4
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    • pp.279-284
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    • 2021
  • Flammulina velutipes is the representative edible mushroom in Korea. It makes up around 18% of the total mushroom production and accounts for about 36% of the total mushroom export. Despite being one of the most industrially important mushroom in Korea, the distribution rate of domestic cultivar was low (~31.6%) in 2020. Therefore, we need a new domestic cultivar with high quality as the main export mushroom. We developed a new white domestic cultivar, 'Seolhan', bred by mating two monokaryotic strains isolated from Baekseung and Woori-1ho. The optimum mycelial growth temperature was 25℃ and Seolhan had higher mycelial growth than the control at 20 and 25℃. In bottle cultivation, the optimum temperature of incubation was from 15℃ to 17℃ and the growth temperature of Seolhan was 7-8℃, which was slightly higher than that of the control. The yield of Seolan was 226.5±8.0 g, an increase of 18.4% compared to the control. The period of primodia formation also was a couple of days ahead. In morphological traits, the pileus of Seolhan was smaller than that of the control, the stipe was thicker, and the color brighter. As the results of replacement culture and genetic diversity test, it was confirmed that Seolhan was genetically different from other white varieties.

Characteristics of newly bred spore-less cultivar Lentinula edodes 'Daedam' for sawdust cultivation (표고 톱밥재배용 무포자 신품종 '대담' 육성 및 특성)

  • Jeong-Han Kim;Young-Ju Kang;Chae-Young Lee;Yeon-Jin Kim;Jun-Yeong Choi;Chan-Jung Lee;Tai-Moon Ha;Gab-June Lim
    • Journal of Mushroom
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    • v.21 no.3
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    • pp.154-159
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    • 2023
  • A new spore-less cultivar Lentinula edodes 'Daedam' was bred from monokaryotic strains of 'LE15401-24' and 'LE192118-10'. The optimum temperature for mycelial growth of 'Daedam' on potato dextrose agar was 22~25℃. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 134 days, similar to that of the control cultivar 'Hwadam'. Total yield of 'Daedam' was 222g per 3kg substrate, and was lower than that of control cultivar(266.0g). The fruiting body of 'Daedam' had a thick and small pileus and a longer stem compare to control cultivar. As a result of a analyzing the productivity of 'Daedam' on the different substrate types, the biological efficiency was 26.7% in the 1.2kg cylindrical substrate(CS), which was higher than that of the 3kg rod-type substrate(RS). 'Daedam' had a similar yield compared to 'Hanacham' in first fruiting body production, but the cultivation period was 40 days shorter. Therefore, 'Daedam' can only harvest fruiting bodies once, it is thought that it can be used as spore-less oak mushroom cultivar for short-term cultivation instead of 'Hanacham' in mushroom farms.

Characteristics of Newly Bred Lentinula edodes Cultivar 'Jadam' for Sawdust Cultivation (표고 톱밥재배용 신품종 '자담' 육성 및 특성)

  • Jeong-Han Kim;IL-Sun Baek;Jong-In Choi;Bok-Eum Shin;Young-Ju Kang;Tai-Moon Ha;Gu-Hyun Jung;Yeon-Jin Kim;Jun-Yeong Choi
    • The Korean Journal of Mycology
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    • v.50 no.2
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    • pp.93-102
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    • 2022
  • A new cultivar of oak mushroom Lentinula edodes 'Jadam' was bred from monokaryotic strains of 'Hwadam-18' and 'GMLE36295-22'. The optimum temperature for mycelial growth of 'Jadam' on potato dextrose agar was 22-25℃. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 122-124 days, shorter to that of the control cultivar 'Hwadam'. Total yield of 'Jadam' was 623.8 g per 3 kg substrate, and is higher than that of 'Hwadam' (455.2 g). In the fruiting body of the new cultivar, the stipe was shorter and thinner than those of 'Hwadam'. A farmhouse field test showed that the period of mycelial growth and browning was shorter than that of control cultivar 'L808'. The total yield for 2 flush was 543.3 g for 'Jadam'. It was similar to the 585.3 g of the control cultivar. Therefore, the new cultivar 'Jadam' could be a substitute for 'L808' in the field of farms.

Characteristics of a new Grifola frondosa Cultivar "Daebak" with stable pinheading and high yield (발이 안정 및 다수성 잎새버섯 신품종 '대박'의 특성)

  • Jeon, Dae-Hoon;Lee, Yun-Hae;Choi, Jong-In;Gwon, Hee-Min;Chi, Jeong-Hyun;Hong, Hye-Jeong;Jang, Kab-Yeul
    • Journal of Mushroom
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    • v.16 no.3
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    • pp.203-207
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    • 2018
  • 'Daebak', a new cultivar of Grifola frondosa, was bred by mating two monokaryotic strains isolated from 'F14309' and 'GMGF44062' at the Mushroom Research Institute, Gyonggi-Do ARES in 2017. The optimum temperature for mycelial growth of 'Daebak' was $25^{\circ}C$ on PDA medium. In bottle cultivation, the culture period of 'Daebak' was 57 days, which was 2 days shorter than that of 'Cham' (control). The pinheading rate of 'Daebak' was 98.4%, which was 24.8% higher than that of 'Cham' and its CV (Coefficient of variation) was 0.6%, 5.3% lower than that of 'Cham'. Regarding the growth characteristics of 'Daebak', the diameter and thickness of the pileus were 27.7 mm and 1.73 mm, respectively, and the diameter and height of the fruiting bodies cluster were 132 mm and 87.2 mm, respectively. The pileus was thinner but the fruiting bodies cluster was larger than that of 'Cham'. Fruiting bodies weighed 139 g per 1,100 ml bottle of 'Daebak', which was 28% higher than that for 'Cham', with a CV of 2.5%, which was 6.2% lower than that of 'Cham'. The yield per 10,000 bottles (used for cultivation) of 'Daebak' was 1,376 kg, 70% higher than that of 'Cham', with a CV of 3.0% that was 11.5% lower than that of 'Cham'. With respect to physical characteristics, the strength and brittleness of the fruiting body of 'Daebak' was less than that of 'Cham'. When considering the period available for sale, the shelf life of 'Daebak' was 42 days, which was 6 days longer than that of 'Cham'.