• Journal of Animal Science and Technology
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• v.64 no.2
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• pp.330-342
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• 2022

We aimed to investigate candidate proteins related to long-term caloric restriction and feed efficiency in bovine longissimus dorsi muscle (LM). A total of 31 Korean native steers were randomly distributed to ad libitum (n = 16) or caloric restriction group (n = 15) to conduct two feeding trials for 13 mon. In the first trial (10-18 mon of age), steers were fed with 100% ad libitum (NE_g = 0.63 Mcal/kg) or caloric restriction (80% of the previous day's feed intake of ad libitum group). In the second trial (18-23 mon of age), the energy value of 100% ad libitum diet was 1.13 Mcal/kg NE_g and those in caloric restriction group diet was 0.72 Mcal/kg NE_g. At the endpoint of this experiment, in each group, 6 animals were selected with high (n = 3) or low feed efficiency (n = 3) to collect muscle tissue samples (6 animals/group). From muscle tissues of 23 mo of age, we excavated 9 and 12 differentially expressed (two-fold or more) proteins in a nutritional group and feed efficiency group using two-dimensional electrophoresis, respectively. Of these proteins, heat shock protein beta-6 was up-regulated in both the caloric restriction and the low feed efficiency group. In bovine embryonic fibroblasts, the mRNA expression of heat shock protein beta-6 increased after adipogenic differentiation, however, decreased after myogenic differentiation. Our data provide that heat shock protein beta-6 may be an adipogenic protein involved in the mechanism of caloric restriction and feed efficiency in the LM of the steer.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.445-451
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• 2021

In this study, we described the production of an antibody to living modified organisms (LMOs) containing the gene encoding for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens strain CP4 EPSPS provides resistance to the herbicide glyphosate (N- (phosphonomethyl) glycine). These LMOs were approved and have recently been used in the feed, food production, and processing industries in South Korea. Highly efficient monoclonal antibody (mAb) production is crucial for developing assays that enable the proper detection and quantification of the CP4 EPSPS protein in LMOs. This study describes the purification and characterization of recombinant CP4 EPSPS protein in E. coli BL21 (DE3) based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrixassisted laser desorption/ionization time-of-flight mass spectrometry. The production of mAbs was undertaken based on the standard operating procedure of Abclon, Inc.(South Korea), and the purity of the mAbs was assessed using SDS-PAGE. The following five mAb clones were produced: 2F2, 4B9, 6C11, 10A9, and 10G9. To verify the efficiency and specificity of the five developed mAbs, we performed Western blotting analysis using the LM (living modified) cotton crude extracts. All mAbs could detect the CP4 EPSPS protein in the LM cotton traits MON1445 and MON88913 with high specificity, but not in any other LM cottons or non-LM cottons. These data indicate that these five mAbs to CP4 EPSPS could be successfully used for the further development of antibody-based detection methods to target CP4 EPSPS protein in LMOs.

• Korean Journal of Mathematics
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• v.27 no.2
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• pp.547-562
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• 2019

In this paper, we define a local topological group-groupoid and prove that if G is a local topological group-groupoid, then the monodromy groupoid Mon(G) of G is a local group-groupoid.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.12
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• pp.1761-1768
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• 2006

In this study, the comparative effects of transgenic corn (Mon 810 and Event 176) and isogenic corn (DK729) were investigated for their influence on in vitro rumen fermentation. This study consisted of three treatments with 0.25 g rice straw, 0.25 g of corn (Mon810/Event176/DK 729) mixed with 30 ml rumen fluid-basal medium in a serum bottle. They were prepared in oxygen free conditions and incubated at $39^{\circ}C$ in a shaking incubator. The influence of transgenic corn on the number of bacterial population, F. succinogenes (cellulolytic) and S. bovis (amylolytic), was quantified using RT-PCR. Fermentative parameters were measured at 0, 2, 4, 8, 12 and 24 h and substrate digestibility was measured at 12 and 24 h. No significant differences were observed in digestibility of dry matter, NDF, ADF at 12 and 24 h for both transgenic and isogenic form of corns (p>0.05) as well as in fermentative parameters. Fluid pH remained unaffected by hybrid trait and decreased with VFA accumulation as incubation time progressed. No influence of corn trait itself was seen on concentration of total VFA, acetic, propionic, butyric and valeric acids. There were no significant differences (p<0.05) in total gas production, composition of gas (methane and hydrogen) at all times of sampling, as well as in NH3-N production. Bacterial quantification using RT-PCR showed that the population number was not affected by transgenic corn. From this study it is concluded that transgenic corn (Mon810 and Event 176) had no adverse effects on rumen fermentation and digestibility compared to isogenic corn. However, regular monitoring of these transgenic feeds is needed by present day researchers to enable consumers with the option to select their preferred food source for animal or human consumption.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.470-470
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• 2015

본 연구의 목적은 위성영상 기반의 SEBAL(Surface Energy Balance Algorithm for Land) 모형과 SWAT(Soil and Water Assessment Tool) 수문모형을 용담댐 유역($922.3km^2$)에 적용하여 증발산량을 산정하고 모형 간 공간 증발산량의 비교를 통해 각 모형의 적용성을 평가하는데 있다. 이를 위해 SEBAL모형의 입력자료로 Terra MODIS(Moderate Resolution Imaging Spectrometer) Product 중 Normalized Distribution Vegetation Index(NDVI), Albedo 영상을 2012년부터 2013년까지 월단위로 구축하고, 일단위의 Land Surface Temperature(LST) 영상을 구축하였다. 지형자료로는 Digital Elevation Model(DEM)과 Land use를 구축하였으며 SEBAL 모형의 구동을 위한 위성영상 및 지형자료는 500 m의 공간해상도로 재구축하였다. SWAT 모형의 모의를 위해 기상 및 유량 자료를 2000년부터 2013년까지 일단위로 구축하였고, DEM, Land use, 토양도의 지형자료를 30 m의 공간해상도로 구축하였다. SWAT 모형의 유출 검보정 후 수위관측소 지점에서 평균 $R^2$를 산정한 결과 도치(0.80), 동향(0.72), 석정(0.64), 주천(0.80), 천천(0.80), 용담댐(0.72)로 높은 상관성을 나타냈으며, 유출 검보정 후 SWAT 모형의 증발산량 모의 결과를 바탕으로 SEBAL 모형과의 공간 증발산량을 비교하였다. 두 모형의 증발산량은 SEBAL 모형의 경우 지형에 따라 SWAT 모형은 토양 특성에 따라 분포하는 경향이 다르게 나타났다. SEBAL 모형은 주로 저지대에서 증발산량이 높게 산정되며 고지대로 갈수록 감소하여 증발산량이 지형의 고저차에 따라 분포하는 모습을 보였다. SWAT 모형은 토양 특성에 따라 증발산량이 분포하며 유역 내에서 뚜렷한 차이를 나타내지는 않았다. 월별 총 증발산량은 SWAT 모형의 경우 7~8월에 약 90 mm/mon로 가장 높게 나타나고 1~2월은 0 mm/mon로 계절별 변화폭이 컸으나, SEBAL 모형의 경우 5~6월에 증발산량이 약 60 mm/mon로 가장 높게 나타났고 계절별 변화 폭이 SWAT 모형에 비해 적은 모습을 보였다. 이는 위성영상을 기반으로 하는 SEBAL 모형의 특성상 장마 기간에 해당하는 7~8월은 구름으로 인해 일사량이 적게 계산되고, 그 결과 5~6월에 비해 증발산량이 작게 산정되는 것으로 판단된다.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.467-469
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• 2010

• Restorative Dentistry and Endodontics
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• v.37 no.4
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• pp.232-235
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• 2012

Hypoesthesia after an inferior alveolar nerve (IAN) block does not commonly occur, but some cases are reported. The causes of hypoesthesia include a needle injury or toxicity of local anesthetic agents, and the incidence itself can cause stress to both dentists and patients. This case presents a hypoesthesia on mental nerve area followed by IAN block anesthesia with 2% lidocaine. Prescription of steroids for a week was performed and periodic follow up was done. After 1 wk, the symptoms got much better and after 4 mon, hypoesthesia completely disappeared. During this healing period, only early steroid medication was prescribed. In most cases, hypoesthesia is resolved within 6 mon, but being aware of etiology and the treatment options of hypoesthesia is important. Because the hypoesthesia caused by IAN block anesthesia is a mild to moderate nerve injury, early detection of symptom and prescription of steroids could be helpful for improvement of the hypoesthesia.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.273-274
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• 2010

• Proceedings of the KSME Conference
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• 2005.11a
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• pp.51.1-51.1
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• 2005

• The Bulletin of The Korean Astronomical Society
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• v.22
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• pp.29.1-29.1
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• 1997