• Applied Biological Chemistry
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• v.46 no.1
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• pp.60-65
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• 2003

MMP-1 inhibitory compounds were isolated from 120 Korean traditional edible plants. UP- 1 activity significantly increased linearly with increasing UVB dose in normal human foreskin fibroblast HS68 cell, showing maximum activity at approximately 35 $mJ/cm^2$, whereas in HaCaT cell, normal human keratinocyte, no increase was observed. Maximum secretion of MMP-1 after UVB treatment occurred around 36-48 k after treatment. MMP-1 inhibitory compound isolated from cold-water fraction of Cataegus pinnatifida Bunge showed the mort potent activity. The MMP-1 inhibitory compound was deduced as a peptide based on the fact that pronase digestion decreased the activity whereas periodate oxidation did not. The most potent UP- 1-inhibitory protein, CP-2Va-2, showing an activity of 88.5% against MMP-1, was isolated through sequential column chromatography on DEAE-Toyopearl 650C, Butyl-Toyopearl 650M, and Bio-Gel P-30. Molecular weight of CP-2Va-2 determined through high performance liquid chromatography and SDS PACE was 19 and 20 kDa. respectively, signifying a monomeric structure.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.149-155
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• 2011

This study was carried out to develop a method of removing unnecessary sugars and high-molecular weight soluble components from water extract by using various highly porous polymer resins: these resins are widely used as adsorbents for polyphenolic compounds. Each anthocyanin-rich fraction (ARF) that was eluted from column packed with 4 different resins (SEPABEADS SP207, 700, 850 and Diaion HP 20) was obtained from an aqueous extract of the ripe fruits of Rubus coreanus, which is well-known in Korea as "Bok-bun-ja". Among the above mentioned resins, Diaion HP20 had the highest efficacy and provided maximum yield of the ARF. The ARF eluted from the column packed with HP 20 exhibited the strongest antioxidant activity in vitro. Bioactive phytochemicals extracted from Rubus coreanus were investigated using the on-line HPLC-$ABTS^{{\cdot}+}$ system, and the resulting 6 peaks were identified as radical-scavenging components. By using liquid chromatography-mass spectrometry, 5 peaks were obtained, and these were identified as cyanidin-3-sambubioside, cyanidin-3-glucoside, cyanidin-3-xylosylrutinoside, cyanidin-3-rutinoside and pelargonidin-3-rutinoside. These results indicate that the use of appropriate porous resin (Diaion HP 20) leads to an increase in the yield of bioactive components and enhancement of their biological properties.

• Journal of Nutrition and Health
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• v.37 no.7
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• pp.533-539
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• 2004

It has been reported that CLA decreases fat deposition in vivo and in vitro experiments. Among CLA isomers, c9t11 and t10c12 have been shown to exert active biological activities. For example, t10c12 reduces body weight and increases lean body mass, whereas, c9t11 has little effect on body fattness. However, the underlying molecular mechanism for the anti-obesity action of CLA isomers are not well understood. The purpose of this study was to examine the effects of t10c12 and c9t11 on lipid accumulation, cell proliferation, cell death and the expression levels of Ucp genes which are proposed as targets for anti-obesity in 3T3-L1 preadipocytes. Isomers of CLA at 50$\mu$M were added into preadipocyte differentiation medium for 3, 6 and 9days. Control cells received only the vehicle in the differentiation medium. Cytochemical analyses for lipid accumulation, cell proliferation and apotosis were carried out to compare lipidogenesis and cellular activity. RT-PCR analysis of GAPDH, Ucp 2,3 and 4 were also performed to find any modulatory effects of CLA isomers on the metabolic genes. Lipid accumulation indicated by Oil Red-O staining was inhibited in CLA isomers as compared to the control. T10c12 isomer showed less lipidogenesis than c9t11 did. A decrease occurred in CLA isomers as shown by BrdU incorporation. Apotosis has occured at higher level in t10c12 when compared to that of t9c11. Ucp 2, 3 and 4 genes were also upregulated in CLA isomers. T10c12 showed higher level of Ucp gene expressions than the c9t11 did. The biological activities of CLA isomers were also found to be different during differentiation of 3T3-L1 preadipocytes, suggesting that different isomers may be active in certain stage of lipidogenesis. The results indicate that both c9t11 and t10c12 CLA isomers decrease lipidogenesis, inhibit cell proliferation, increase cell death and upregulate in Ucp gene expressions during 3T3-L1 preadipocyte differentiation. T10c12 isomer was more effective than c9t11 in overall anti-obesity activity.

• Journal of Conservation Science
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• v.27 no.2
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• pp.135-144
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• 2011

When metal artifacts have to undergo conservation treatment, the person in charge of the treatment selects and uses various coating mixtures based on his judgment regarding their condition, material, or environment. Since the kinds of coating mixtures or solvents make a difference in the set time of cyanoacrylate adhesives, they have something to do with the efficiency of the conservation treatment. This study examines the effects and causes that affect the set time of cyanoacrylate adhesives according to the kinds of coating mixtures and solvents and suggests ways to increase the set time. As a result, it is thought that as the surface roughness gets flatter, the wettability of adhesive is improved further, which increases the set time. Moreover, the C-F binding of V-Flon, C-O-C absorption peak, molecular weight of the coating mixtures, and glass transition temperature (Tg) were the factors that significantly affected the set time. According to the result of measuring the set time based on the result of superficial and chemical analysis, relative difference was shown according to the kind and viscosity of adhesive, but all the adhesives indicated the following order of the set time: V-Flon > Paraloid B-72 (in xylene) > Paraloid NAD-10 > Paraloid B-72 (in acetone).

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.18 no.1
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• pp.116-134
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• 2005

Although the parallel prescription of Sopoongsangagambang (SG) administration along with external treatment such as spraying or ointment application on the skin is clinically used for the treatment of atopic dermatitis (AD), molecular mechanism underlying its effectiveness is unknown. Thus in the present study, diverse immune responses in terms of chemical mediators related to AD were investigated using an atopic mouse model NC/Nga after SG administration and external treatment (ET), and major findings are summarized as follows. 1. The clinical severities in 16 and 20 week old NC/Nga mice with SG and ET treatment were decreased to 72.2% and 62.3% respectively compared to the control NC/Nga mice with no drug treatment. 2. IgE, IL-4, IL-5, IL-6, IL-13, IgM, IgG1 and IgG2a levels in the serum of SG and ET treated NC/Nga mouse group were significantly decreased compared to the untreated control mice. In contrast, $IFN-{\gamma}$ showed a significant increase in the experimental group compared to the untreated control group. 3. The spleen weight of SG and ET treated NC/Nga mice was significantly decreased compared to the untreated control group. 4. The B/T ratio in the lymph node of SG and ET treated NC/Nga mice was increased compared to the untreated control group. $CD4^+\;and\;CD8^+$ cell numbers in the lymph node of SG and ET treated NC/Nga mice were significantly increased compared to the untreated control group, but $CD69^+\;and\;CD11a^+$ cells were significantly decreased. 5. mRNA expression levels of IL-4, IL-5, and CCR3 in the skin tissues of SG and ET treated NC/Nga mice were significantly decreased, and expression levels of IL-6, IL-13, $CD69^+/CD3{\varepsilon}^+\;and\;CD19^+/CD44^+$ in the skin tissues of SG and ET treated NC/Mga mice were significantly decreased compared to the untreated control group. $IFN-{\gamma}$ mRNA expression levels were increased compared to the untreated control group. 6. Histological observation of the ear and neck skin tissues showed that the extents of inflammation and infiltrated immune cells in the epidermis and dermis of SG and ET treated NC/Nga mice were highly reduced compared to the untreated control group. 7. Lymphokine assay showed a significant decrease in IL-4 levels in SG and ET treated NC/Nga mice compared to the untreated control group, but the levels of $IFN-{\gamma}$ secretion were significantly increased drug treated NC/Nga mice.

• Journal of the Korean GEO-environmental Society
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• v.9 no.7
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• pp.5-11
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• 2008

The paper presents the results of qualitative analysis of phthalate esteres in Miho stream in of Cheongju city. Phthalate esters (Di-2-ethylhexylphthalate, Di-n-butylphthalate) are widely used as plasticizers to increase the flexibility and workability of high-molecular-weight polymer. For water phase, DEHP concentration in upper stream and down stream were $12-18{\mu}g/{\ell}$ and $11-21{\mu}g/{\ell}$, respectively. For sediment phase, DEHP concentration in upper stream and down stream were $0.07-0.82{\mu}g/g$ (dry) and $0.06-0.92{\mu}g/g$ (dry), respectively. Also, DnBP concentration of sediment in same site were $0.04-0.25{\mu}g/g$ (dry) and $0.08-0.34{\mu}g/g$ (dry), respectively. DEHP and DnBP concentration of water phase in the small stream of industrial area were $13-28{\mu}g/{\ell}$ and $2-8{\mu}g/{\ell}$, respectively. DEHP and DnBP concentration of sediment phase in the small stream of industrial area were $0.12-0.7{\mu}g/g$ (dry) and $0.17-2.16{\mu}g/g$ (dry), respectively. Phthatlate esteres in water and sediments phase of Miho stream were lower than water bodies around the world.

• Journal of the Korea Concrete Institute
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• v.28 no.1
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• pp.59-65
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• 2016

Commercial synthetic resins with great amount of hydrogen atoms were investigated for neutron shielding aggregates. Total three types of resins were considered in this study: high density polyethylene (HDPE), polypropylene (PP), and ultra molecular weight polyethylene (UPE). When these resins replaced 20, 40, 60 vol% of fine aggregates, mechanical properties were first evaluated including compressive and tensile strengths, and then image/microstructure analyses such as cross-section analysis, SEM, and X-ray CT were performed. The results showed that the compressive and tensile strengths decreased with the increase of replacement ratio of HDPE and PP, which was found through image analysis that it was closely related to the distribution of resins at the failure surface of test specimens. The strength reduction of UPE was quite small compared to HDPE and PP but it abruptly increased when the replacement level exceeded 60 vol%. The results of microstructure analyses indicated that the replacement level significantly affected the amount of air void so that it is critical to determine the reasonable amount of UPE to make cementitous materials for neutron shielding.

• Korean Journal of Food Science and Technology
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• v.25 no.1
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• pp.39-45
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• 1993

The effects of enzymatic modification with pepsin and actinidin was studied on molecular weight distributions and functional properties of hydrolysates from soy protein isolate (SPI) differing in degree of hydrolysis. The hydrolyzed SPI by pepsin showed 41.5% degree of hydrolysis after 5 min, and maximum hydrolysis was obtained after 2 hours. Actinidin hydrolyzed SPI 26.71% degree after 1 hour. On SDS-PAGE, native SPI showed 9 distinguishable bands on SDS-PAGE gel. Pepsin treated SPI showed one broad band in the lower part of gel. This band was shifted further to the bottom of the gel and became faint as hydrolysis time increased. While actinidin treated SPI showed different SDS-PAGE pattern from pepsin. However PAGE patterns were similar with pepsin and actinidin treated groups. With pepsin treatment, solubility of SPI distinctively increased around isoelectric point(pI). Emulsifying activity (EA) and emulsifying stability (ES) showed marked increase over pH range of $3.0{\sim}8.0$. 5 min modified group had most excellent foam expansion (FE). Foam stability (FS) was increased as pepsin treatment time increased at pI. With actinidin treatment, solubility was increased. 60 min modified SPI had the most effective EA at pH 4.5. However ES was not effected by actinidin treatment. 5 min modified group was most effect in FE. FS was higher at alkaline pH.

• Korean Journal of Food Science and Technology
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• v.24 no.1
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• pp.1-6
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• 1992

Changes in protein distributiun, eletrophoretic patterns and amino acid composition were investigated during germination of malting barley. Fractionation of the protein complex in ungerminated malting barley resulted in a higher hordein fraction but less glutelin fraction of the protein complex in ungerminated malting barley resulted in a higher hordein fraction but less glutelin fraction as compared to germinated malting barley. As germination proceeded, NPN, globulin and glutelin fractions continued to increase, accmpanied by decreases in albumin and hordein fractions. The electrophoretic pattern of malting barley proteins showed three bands (molecular weight range of $15,000{\sim}41,000$ daltons) in albumin fraction, five bands ($19,000{\sim}61,000$ daltons) in globulin fraction, five bands ($18,000{\sim}56,000$ daltons) in hordein fraction and tour bands ($20,000{\sim}47,000$ daltons) in glutelin fraction, exhibiting quantitative changes in each fraction during germination. Amino acid analysis showed that glutamic acid, histidine, aspartic acid, serine, glycine, valine, alanine and leucine were major amino acids of proteins in malting barley grains. Glutamic acid increased slightly, but other amino acids showed no definite trend as germination proceeded.

• Elastomers and Composites
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• v.47 no.1
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• pp.54-64
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• 2012

Acrylonitrile was introduced in the emulsion SBR to increase compatibility between silica and rubber. AN-SBR/silica compounds showed faster vulcanization time and higher delta torque values than SBR 1721/silica compounds because interaction between nitrile group of AN-SBR and silanol group on the silica surface could make hydrogen bond that prevented adsorption of the accelerator on the silica surface, which improved the vulcanization reaction efficiency and enhanced the degree of crosslinking. AN-SBR/silica compound showed higher values in minimum torque than SBR 1721/silica compound during the vulcanization because AN-SBR has higher molecular weight than SBR 1721 in the raw material. When PEG was added to the SBR 1721 and AN-SBR compounds, vulcanization time was faster than SBR 1721 and AN-SBR compounds without PEG because PEG has a large number of ether linkages which show high compatibility with silanol group on the silica surface that prevented the adsorption of the accelerator and the ingredients on the silica surface, which improved the vulcanization reaction efficiency. In the mechanical properties, AN-SBR compounds showed higher modulus values at 100%, 300% than SBR 1721 compounds because interaction between nitrile group of AN-SBR and silanol group on the silica surface enhanced the degree of crosslinking. In the dynamic properties, AN-SBR compounds showed lower tan ${\delta}$ values at $0^{\circ}C$ than SBR 1721 compounds in accordance with the $T_g$ values. AN-SBR compounds showed lower tan ${\delta}$ values at $60^{\circ}C$ than SBR 1721 compounds because interaction between acrylonitrile and silica caused strong filler-rubber interaction that induced low energy dissipation by the filler-filler interaction.