• Journal of Marine Bioscience and Biotechnology
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• v.6 no.2
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• pp.62-67
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• 2014

In this study, we investigated the bioactive substances of the Alcalase hydrolysate obtained from fishery processing by-products in Jeju by measuring bioactivities including radical scavenging acitivty, cytoprotective activity against 2,2-azobis-(2-amidino-propane) dihydrochloride (AAPH), and ACE inhibitory activity. This study is important because of utilization of unused fishery processing by-products in Jeju. The Alcalase hydrolysate was prepared through the hot water extraction and enzymatic hydrolysis, and then further separation of the Alcalase hydrolysate was performed by ultrafiltration using 10 kDa molecular weight cut-off membrane. The Alcalase hydrolysate showed the relatively higher DPPH and peroxyl radical scavenging activity ($IC_{50}$ value; 1.30 mg/ml and 0.888 mg/ml, respectively). Also, the Alcalase hydrolysate showed the ACE inhibitory activity with 1.87 mg/ml of $IC_{50}$ value. These biological activities are increased over 1.2 or 2.5 times through the ultrafiltration of the Alcalase hydrolysate. Therefore, the Alcalase hydrolysate obtained from fishery processing by-products in Jeju and the different molecular weight fractions should be given consideration for food and cosmetics ingredient. Furthermore, this research on the utility of fishery processing by-products might be a useful tool into the industry.

• Membrane Journal
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• v.10 no.2
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• pp.92-101
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• 2000

For selective elimination of salt in the boiled extract of baby neck clam (Tapes variegata, BEBNC), electrodialyzer was used and the desalination conditions of BEBNC were determined at various concentrations, pH's and volumes of BEBNC. The ion-exchange membrane with a molecular weight cut off 100 Da was used for desalting of BEBNC. The desalination times on BEBNC concentrations of 0.5％ and 4％ were 100 min and 170 min and the final desalting ratio was above 90％. 1'he desalination of BEBNC at pH 4.0 was 12％ higher than that at pH 9.0 for 60 min. The electrodialysis process could remove above 90％ of the initial salt content in 2％ BEBNC of 1 L for 80 min. The results obtained prove that electrodialysis is a practical solution to the problem of selective elimination of salt from BEBNC.

• KSBB Journal
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• v.11 no.3
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• pp.347-352
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• 1996

The enzyme deactivation in a membrane recycling system for the simultaneous saccharification and fermentation(SSF) was studied under various temperature and pressure. The optimum molecular weight cut off(MWCO) of the ultrafiltration membrane for recycling cellulase and ${\beta}$-glucosidase was 50,000. When the cellulase was recycled continuously through the membrane system, it was not deactivated. But the activity of ${\beta}$-glucosidase was decreased with an increase in operating temperature and transmembrane pressure. After 720 minutes at $42^{\circ}C$ and 24.8 psig , the activity of ${\beta}$-glucosidase was reduced by 35% of the initial activity. Such tendencies could be well explained by the results of highly induced shear at the fiber surface of membrane when temperature and transmembrane pressure became higher.

• Journal of Korean Society of Water and Wastewater
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• v.14 no.3
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• pp.224-230
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• 2000

Membrane processes are capable of removing much materials from water. The removal or rejection characteristics of a membrane is usually depend upon the nominal pore size or MWCO(molecular weight cut off). A membrane with a smaller nominal pore size or MWCO should be capable of removing smaller contaminants from water. A series of experiments was performed to investigate the separation characteristics of membrane processes which consisted of microfiltration(MF) and nanofiltration(NF). To evaluate removal efficiencies of some pollutants such as the consumption of $KMnO_4$, THMFP, NH3-N, Fe, Mn, and pesticides, source water sampled from the Kum river was treated by the those membrane processes. Also, the results of experiments were compared with those of conventional water treatment processes. By two types of the membrane process, total removal efficiency of $KMnO_4$ consumed, THMEP, and $NH_3-N$ were 91.0%, 84.3%, and 85.5%, respectively and those processes were efficient in pesticides removal as well. Most of the effluents satisfied the Korean standard of drinking water quality continuously in the experimental periods. However, NF was needed for producing the safe drinking water in case of treating the raw water contaminated with Mn since removal efficiency of MF was not high enough. On the basis of the experimental results, it was suggested that NF could be applied to remove not only $NH_3-N$ but THMFP even without pre-chlorination.

• Preventive Nutrition and Food Science
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• v.5 no.4
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• pp.197-199
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• 2000

Clarified fruit and vegetable juices (apple, carrot and tangerine) were produced using ultrafiltration and their color change due to the mixing ratio were evaluated. clarification was carried out by passing he supernatant of extracted juice through a filter and also by using a membrane of molecular weight cut-off 10,000 Daltons to obtain the juice ultrafiltrates. The mixing ratio between apple and carrot juices was kept constant at 1:1 while increasing the amount of tangerine juice according to 10, 20, 30, 40 and 50% an stored at 4$^{\circ}C$ prior to the color measurement. Hue angle ({TEX}$h_{ab}${/TEX}) and {TEX}$L^{*}${/TEX}-value increased as the tangerine mixing ratio increased. The color difference indicated by ΔE-value also increased as the amount of tangerine increased indicating that the color of the mixed juice became pale and the changes were slight but distinctive. On the other hand, chroma ({TEX}$C^{*}${/TEX}), {TEX}$La{*}${/TEX}- and {TEX}$b^{*}${/TEX}-values decreased as the tangerine mixing ratio increased indicating that the color of the mixed juice became slightly more grayish and the samples were becoming les yellow. A simple mathematical model to predict each color characteristic is proposed.

• KSBB Journal
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• v.23 no.2
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• pp.169-176
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• 2008

The concentration of fresh gel from Aloe vera L. by using ulfrafiltration (UF) process was investigated and analyzed. The two membranes (organic and ceramic) with different molecular weight cut-off (MWCO) and modules (flat sheet and tubular) was used. Under optimum operation conditions, ceramic (zirconium dioxide) tubular membrane with MWCO of 50 kDa resulted in higher flux, less fouling, more turbid, higher total solid, higher polysaccharide and less aloin content. Optimum operation conditions were transmembrane pressure of 1.0 bar, feed velocity of 240 L/hr and temperature of $23^{\circ}C$. Volume concentration factor of aloe gel was 3.13 at permeate flux of $51.1\;L/m^2{\cdot}hr$ after processing time of 1.66 hr. Aloin in fresh aloe gel by UF process was effectively removed as permeate and bioactive polysaccharide content was 2.1 times higher than that of fresh aloe gel. These results allowed a very good level of concentration degree and polysaccharide content. Thus, ultrafiltration process of this study was suitable for the concentration of fresh aloe gel though the aloe concentrate showed both the viscosity decrease and partially separation of liquid layer during storage at $4^{\circ}C$.

• Toxicological Research
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• v.31 no.1
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• pp.33-40
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• 2015

Bee venom (BV) has long been used in traditional Eastern and Western medicine for chronic inflammation, pain and skin therapy. Human exposure to BV, however, often causes unwanted adverse effects and is even fatal in some cases. Phospholipase $A_2$ ($PLA_2$) of BV is now suspected to play a key role in these adverse effects. We investigated the potential use of $PLA_2$-free bee venom (PBV) as a replacement for BV in cosmetic products. PBV prepared by molecular weight cut-off ultrafiltration exhibits a superior profile in comparison with regular BV, by inhibiting elastase activity and suppressing the induction of nitric oxide (NO) and metalloproteinase-9 (MMP-9), while retaining the effects of cell proliferation and protection against ultraviolet B (UVB)-induced damage in human dermal fibroblast cells. PBV thus appears to be more promising than BV as a cosmetic ingredient with a reduced potential for adverse reactions in the recipient.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.04a
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• pp.128-131
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• 2003

It is well known that the membrane separation process combined with surfactant micelle (micellar-enhanced ultrafiltration) or polyelectrolyte (polyelectrolyte-enhanced ultrafiltration) can remove heavy metals effectively. However, the environmental hazard of surfactant or polyelectrolyte remained in effluent is a serious disadvantage of these methods. In this study, humic substances (HS) were used as complexing agents for metal removal instead of synthetic chemicals. The HS are a sort of natural organic matters which are biodegradable and abundant in natural environment. And the functional groups such as carboxyl groups and phenols in HS can bind with the cationic radionuclides and form complexes. Therefore separation process using them will be more environmental-friendly. The effects of concentration of HS and pH on the removal of cobalt were investigated. The ultrafiltration process was applied to the separation of the cobalt - HS complexes from the aqueous stream. At the concentration of > 3 g/L of HS and pH of 6, over 95 % of cobalt was removed by regenerated cellulose membrane of molecular weight cut-off (MWCO) 3,000. As the concentration of HS increased, the removal of cobalt also was improved because of increase in biding sites (functional groups). The cobalt removal increased from 72.5 % to 97.5 % when pH increased from 4 to 8 at the concentration of 3 g/L HS because of increase in HS solubility and cobalt hydroxide precipitation. In the presence of NaCl, the removal efficiency of cobalt decreased.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 1999.11b
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• pp.61-69
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• 1999

Contaminants such as fatty acids, triglycerides, resin acids and foam collected from a high yield sulfite weak liquor storage tank lowered the water surface tension and reduced inter-fibre bonding but also tended to benefit sheet opacity. Some common wet end additives such as defoamers and dispersants gave similar results. Lignosulfonate and naphthalene sulfonate showed little if any negative effect on both surface tension and sheet strength properties. Among the natural wood extractives. fatty acids were identified to be most detrimental followed by triglycerides and then resin acids. In order to alleviate the detrimental impact of these contaminants, membrane separation, air floatation and ozone treatment were carried out on paper machine white water samples. The effect of these treatments on removal of fatty and resin acids was quantified by a GC-Mass analysis. Reverse osmosis with a 1000 molecular weight cut off membrane failed to totally reject fatty and resin acids, but markedly reduced losses of sheet properties due to contaminants. Ozone treatment resulted in a significant increase of the surface tension and air floatation was considered to be a practical and useful method for removing fatty and resin acids from the machine white water.

• Membrane Journal
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• v.12 no.3
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• pp.165-170
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• 2002

Membrane process was investigated to recover useful components, such as protein from rice-washing water generated in the production of the washed-rice. The filtration experiments were carried out using not only a dead-end Amicon cell to determine suitable membranes but also a hollow fiber ultrafiltration, spiral wound nanofiltration and reverse osmosis modules for home water purification. Ultrafiltration module(molecular weight cut-off : 10,000 dalton) was not suitable for recovery of useful components or protein in the rice-washing water, but nanofiltration and reverse osmosis modules showed a good performance. in the case of 250% concentration of the rice-washing water contained about 9% protein the proteins in concentrates of nanofiltration and reverse osmosis were 18% and 22%, which were about 2 and 2.4 times higher protein concentrations than those of feed, respectively.