• 대한핵의학회지
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• 제38권2호
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• pp.131-139
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• 2004

Small animal models are extensively utilized in the study of biomedical sciences. Current animal experiments and analysis are largely restricted to in vitro measurements and need to sacrifice animals to perform tissue or molecular analysis. This prevents researchers from observing in vivo the natural evolution of the process under study. Imaging techniques can provide repeatedly in vivo anatomic and molecular information noninvasively. Small animal imaging systems have been developed to assess biological process in experimental animals and increasingly employed in the field of molecular imaging studies. This review outlines the current developments in nuclear medicine imaging instrumentations including fused multi-modality imaging systems for small animal imaging.

• BMB Reports
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• 제44권11호
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• pp.705-712
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• 2011

Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological/chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제41회 하계 정기 학술대회 초록집
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• pp.63-63
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• 2011

In this tutorial session, the field of organic photovoltaic (OPV) cells based on small molecular weight materials will be presented. The previously reported studies on the fabrication, structure, and property of the cells as well as the molecular materials are included. Especially, the factors hampering further enhancement in the power conversion efficiency of the cells such as exciton recombination, light absorption and interfacial morphology between electron donor and acceptor layer will be discussed in detail. The recent progress in our group will also be presented. It includes typical materials and cell fabrication techniques we used as well as the studies on improving the light absorption in the electron donor layer and reducing the extinction of excitons formed by introducing the nanostructured interface between organic layers.

• BMB Reports
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• 제47권3호
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• pp.149-157
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• 2014

The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. Chemical proteomics is an emerging field that extends these types proteomic profiling. In particular, activity-based protein profiling (ABPP) utilizes small-molecule probes to monitor enzyme activity directly in living intact subjects. In this mini-review, we summarize the unique roles of smallmolecule probes in proteomics studies and highlight some recent examples in which this principle has been applied.

• Macromolecular Research
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• 제8권2호
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• pp.59-65
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• 2000

We simulated the conformational changes of polymethacrylates which have side chains with different lengths (methyl and butyl) by molecular dynamics simulation technique. Bulk states of atactic amorphous polymers relaxed at a higher temperature were generated. The chain behaviors of polymethacrylates were investigated upon varying temperatures. Molecular structural information was then obtained by characterizing radial distribution function(RDF), mean square displacement, self diffusion constant, and Connolly surfaces, among others. The estimated self diffusion constants and RDF values of PMMA and PBMA were found to be in good agreement with our expectation in view of the chain flexibility.

• Journal of Chest Surgery
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• 제54권5호
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• pp.433-435
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• 2021

Pleural adhesions are a major challenge in standard and nonintubated video-assisted thoracic surgery. The currently available imaging techniques help to assess the presence and extent of pleural adhesions, but do not provide information on tissue deformability, which is crucial for intraoperative management. In this report, we describe the utilization of real-time elastography mapping of pleural adhesions. This technique enabled us to detect areas with softer adhesions, and helped establish the surgical plan in a difficult case of a patient scheduled for nonintubated video-assisted thoracic surgery.

• BMB Reports
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• 제56권8호
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• pp.426-438
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• 2023

Muscle wasting in end-stage renal disease (ESRD) is an escalating issue due to the increasing global prevalence of ESRD and its significant clinical impact, including a close association with elevated mortality risk. The phenomenon of muscle wasting in ESRD, which exceeds the rate of muscle loss observed in the normal aging process, arises from multifactorial processes. This review paper aims to provide a comprehensive understanding of muscle wasting in ESRD, covering its epidemiology, underlying molecular mechanisms, and current and emerging therapeutic interventions. It delves into the assessment techniques for muscle mass and function, before exploring the intricate metabolic and molecular pathways that lead to muscle atrophy in ESRD patients. We further discuss various strategies to mitigate muscle wasting, including nutritional, pharmacological, exercise, and physical modalities intervention. This review seeks to provide a solid foundation for future research in this area, fostering a deeper understanding of muscle wasting in ESRD, and paving the way for the development of novel strategies to improve patient outcomes.

• 생명과학회지
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• 제25권7호
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• pp.839-848
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• 2015

분자 마커는 유기체에서 다른 유기체와 분자적 수준에서 식별하는 마커이다. 유전적 분석을 위한 분자 마커의 발달은 식물 유전학, 다양한 구조와 가능을 이해하는데 기여하였다. DNA 마커는 임의유전자 증폭에서 다형성을 탐지하는 기법이나 방법(예를 들면 서든 블로팅, 핵산 교잡법, PCR을 이용한 중합효소 연쇄 증폭 반응, DNA 서열화)으로 RFLP, AFLP, RAPD, SSR, SNP 등을 이용하였고 현재에도 이용하고 있다. 최근 기능성 유전자를 이용한 기능성 마커가 각광을 받고 있다. 기능성 마커는 다형성 서열에서 유래한 것으로 표현형 변이를 내포하고 있다. 이런 개념에서 출발한 기능성 마커는 모든 유전자를 타깃으로 할 수 있으나 식물에서는 P450, 튜블린 형성 유전자의 다형성(TBPs), 전이요소 마커(TEMs), 병원균 저항성 유전자 마커(RGMs), RNA를 기반으로 한 마커(RBMs) 등이 널리 이용되고 있다. 본 연구는 Poczai 등의 총설을 기반으로 구성하였다. 식물에서 이런 분자 마커의 이용은 식물의 분화, 진화, 생리적 기능성 유전자의 변화 등 생물학 전반에 관한 정보 획득에 도움을 될 것이다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1446-1449
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• 2008

Immune system is composed of multiple cells with distinct functions, and immune responses are orchestrated by complex and dynamic cell-cell interactions. Therefore, each cell behavior and function should be understood under right spatio-temporal context. Studying such complexity and dynamics has been challenging with conventional biological tools. Recent development of new technologies such as state of art imaging instruments and microfabrication techniques compatible with biological systems have provided many exciting opportunities to dissect complex and dynamic immune cell interactions; new microscopy techniques enable us to observe stunning dynamics of immune system in real time. Microfabrication permits us to manipulate microenvironments governing molecular/cellular dynamics of immune cells to study detailed mechanisms of phenomena observed by microscopy. Also, microfabrication can be used to engineer microenvironments optimal for specific imaging techniques. In this presentation, I am going to present an example of how these two techniques can be combined to tackle challenging problems in immunology. Obviously, this strategy can readily be applied to many different fields of biology other than immunology.

• Investigative Magnetic Resonance Imaging
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• 제25권4호
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• pp.218-228
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• 2021

Due to their high degree of freedom to transfer and acquire light, fiber optics can be used in the presence of strong magnetic fields. Hence, optical sensing and imaging based on fiber optics can be integrated with magnetic resonance imaging (MRI) diagnostic systems to acquire valuable information on biological tissues and organs based on a magnetic field. In this article, we explored the combination of MRI and optical sensing/imaging techniques by classifying them into the following topics: 1) functional near-infrared spectroscopy with functional MRI for brain studies and brain disease diagnoses, 2) integration of fiber-optic molecular imaging and optogenetic stimulation with MRI, and 3) optical therapeutic applications with an MRI guidance system. Through these investigations, we believe that a combination of MRI and optical sensing/imaging techniques can be employed as both research methods for multidisciplinary studies and clinical diagnostic/therapeutic devices.