• Journal of Conservation Science
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• v.5 no.2 s.6
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• pp.51-56
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• 1996

This paper deals with the restoration of ancient wooden bridge foundation which excavated in Seta river Shiga Prefecture, Japan. Shiga Archeological Research started a marine archeological investigation of the bridge foundation in 1987. The bridge foundation stricture excavated and have since then recovered about a lots of woods and another materials. The bridge foundation structure constructed log, timbers and stones. The species of those waterlogged wood were identified as two types, hardwood and softwood. Hardwood(log : Cyclobalanopsis) was used for below foundation and softwood (timber' Chamaecyparis obtusa Endl. Cupreessaceae) was used for base structure. One of those timber sample dated by dendrochronology, we asked Dr. Misutani*. The softwood gave a felling date of 567 A.D. In result, the ancient Seta bridge foundation structure had constructed between Asuka and Nara period. We healed the news that ancient bridge foundation excavated at Woljyongyo site in Kyongju, Korea 1987. The bridge foundation Setakarahashi is similar in plane and structure to Woljyongyo structures. The Woljyongyo site report had be of value for reference. We had planning to restore those woods. Hardwood log was got serious damage. The water content varies from 400 to $600\%$. The other timbers water content varies about $200\%$. In the Shiga Center for Archaeological Operations and the Azuchi Castle Archaeological Museum, we set up the PEG impregnation tank. Those wooden objects treated by PEG method. PEG with a molecular weight of 4000. The treatment results may be considered satisfactory. The ancient wooden Seta bridge was reconstructed in Biwako Museum which established in Oct. 1996. We must take care of indoor exhibition environments. (*Nara National Cultual Properties Research Institute).

• Analytical Science and Technology
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• v.26 no.1
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• pp.61-66
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• 2013

The activity of an antimicrobial peptide, protegrin-1, on the well-aligned lipid bilayer deposited on a thin coverglass plate was investigated by $^2H$ solid-state NMR spectroscopy. Orientational distribution and molecular motion in the lipid bilayer were determined from $^2H$ solid-state NMR spectrum. Reorientational motion of lipid molecules in the vacuum-dried state was found to be small but their orientational distribution was not able to be determined. As storage times were longer, the order of the alignment of lipid molecules in the lipid bilayer and percentages involved in the toroidal pore structures increased. We found that much longer time is required to get the equilibrium state of the peptide-lipid mixture under our experimental condition for investigating the action of the antimicrobial peptide like protegrin-1 on the lipid bilayers deposited on the thin coverglass plates.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2005.11a
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• pp.417-421
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• 2005

Novel two synthetic technics using cationic ring-opening copolymerization of tetrahydrofuran (THF) and ethylene oxide (EO), or just polymerized EO on Poly-THF, could lead to random hydroxyl-terminated poly(EO-ran-THF) or tri-block PEG-PTHF-PEC, respectively. These reactions were carried out using $BF_3O(C_2H_5)_2$ as catalyst, 1,4-butanediol or PTHF as diol initiator. Copolymer structures were controlled by monomer feed ratio, or initial PTHF and EO monomer added amount. The molecular weight of polymer was merely dependant on the ratio of [monomer]/[diol], but not on catalyst. Well-defined random and block hydroxyl-terminated copolyether was found to be as the prepolymer for the propellant binder from the experiment to polyurethane with them.

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.659-665
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• 2016

The oligosaccharides in human milk constitute a major innate immunological mechanism by which breastfed infants gain protection against infectious diarrhea. Clostridium difficile is the most important cause of nosocomial diarrhea, and the C-terminus of toxin A with its carbohydrate binding site, TcdA-f2, demonstrates specific abolishment of cytotoxicity and receptor binding activity upon diethylpyrocarbonate modification of the histidine residues in TcdA. TcdA-f2 was cloned and expressed in E. coli BL21 (DE3). A human milk oligosaccharide (HMO) mixture displayed binding with TcdA-f2 at 38.2 respond units (RU) at the concentration of 20 μg/ml, whereas the eight purified HMOs showed binding with the carbohydrate binding site of TcdA-f2 at 3.3 to 14 RU depending on their structures via a surface plasma resonance biosensor. Among them, Lacto-N-fucopentaose V (LNFPV) and Lacto-N-neohexaose (LNnH) demonstrated tight binding to TcdA-f2 with docking energy of −9.48 kcal/mol and −12.81 kcal/mol, respectively. It displayed numerous hydrogen bonding and hydrophobic interactions with amino acid residues of TcdA-f2.

• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1036-1042
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• 2011

The exopolysaccharide botryosphaeran ($EPS_{GLC}$; a ($1{\rightarrow}3$)($1{\rightarrow}6$)-${\beta}$-D-glucan from Botryosphaeria rhodina MAMB-05) was sulfonated to produce a water-soluble fraction ($EPS_{GLC}$-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction ($EPS_{GLC}$-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and $^{13}C$ nuclear magnetic resonance ($^{13}C$ NMR) spectroscopies. $EPS_{GLC}$ and $EPS_{GLC}$-RS were also assayed for anticoagulation activity, and $EPS_{GLC}$-RS was identified as an anticoagulant.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.626-632
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• 2009

An Edutainment Contents help the learners to recognize the problems effectively, grasp and classify important information needed to solve the problems and convey the contents of what they have learned. Owing not only to this game-like fun but also to the educational effect, The Edutainment contents can be usefully applied to education and training in the areas of scientific technology and industrial technology. This study proposes the Edutainment that users can apply to biotechnology by using intuitive multi-modal interfaces. In this study, a stereoscopic monitor is used to make three dimensional molecular structures, and multi-modal interface is used to efficiently control. Based on the system, this study easily solved the docking simulation function, which is one of the important experiments, by applying these game factors. For this, we suggested the level-up concept as a game factor that depends on numbers of objects and users.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.59-65
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• 2009

An Edutainment Contents help the learners to recognize the problems effectively, grasp and classify important information needed to solve the problems and convey the contents of what they have learned. Owing not only to this game-like fun but also to the educational effect, The Edutainment contents can be usefully applied to education and training in the areas of scientific technology and industrial technology. This study proposes the Edutainment that users can apply to biotechnology by using intuitive multi-modal interfaces. In this study, a stereoscopic monitor is used to make three dimensional molecular structures, and multi-modal interface is used to efficiently control. Based on the system, this study easily solved the docking simulation function, which is one of the important experiments, by applying these game factors. For this, we suggested the level-up concept as a game factor that depends on numbers of objects and users.

• Membrane Journal
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• v.25 no.4
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• pp.310-319
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• 2015

Three kinds of anion-exchangeable functional groups with different hydrocarbon molecular structures were introduced to vinyl benzyl chloride-based membrane to understand the effect of attached function in anion-exchange membrane. Trimethylamine (TMA) as an aliphatic fuction, N-methylpiperidine (MP) as an alicyclic fuction and pyridine (Py) as an aromatic function were introduced by amination. The respective reactivity was observed by the trace of membrane resistance( MER)/ion exchange capacity (IEC) and the increasing order of reactivity was Py < MP < TMA. Meanwhile, SEM photograph showed the attached Py ion-exchange membrane was the most homogenous and compact structure in the study. In electrochemical properties, the attached Py ion-exchange membrane showed the MER ($5.0{\Omega}{\cdot}cm^2$ >, in 0.5 mol/L NaCl), comparable to those of commercial membrane (AMX). All results showed that the resonance structure of attached functional group might contribute to the preparation of homogenous anion-exchange membrane.

• Journal of the Korean Chemical Society
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• v.34 no.2
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• pp.130-135
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• 1990

The title compound (C13H17NO2S) is monoclinic, space group P21/a, with a = 13.756 (3), b = 9.310(4), c = 21.305(3) $\AA$, $\beta$ = 95.0。, Z = 8, V = 2718.11 $\AA$3, Dc = 1.23$g·{\cdot}cm-3$, (Mo k$\alpha$) = 0.71069$\AA$,$\mu$ = 2.18 cm-1, F(000) = 1071.86, T = 298, R = 0.085 for 2935 unique observed reflections with I >2.0$\sigma$(I). The structure was solved by direct methods. The C-H bond lengths and the methyl groups are fixed and refined as their ideal geometry by allowing to ride on the parent atoms. Both molecules A and B have almost same structures except for two terminal ethyl groups. The ethylene-like skeleton including the nitro group in one molecule is nearly perpendicular to the plane of the methylbenzene group and two ethyl groups form a cis-type structure which has the dfferent orientations between two molecules; in the molecule A, two terminal methyl groups being the opposite directional arrangement against the plane of its skeleton, while in the B, with the same directional structure from its plane. The molecules in the crystal are packed together by non-bonded van der Waals forces.

• Journal of the Korean Chemical Society
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• v.39 no.5
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• pp.329-337
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• 1995

Energetic oxetane derivatives which undergo cationic polymerizations have been investigated theoretically by using ab initio HF/3-21G calculations. We have examined structures, charges, and molecular electrostatic potentials. The ring structure of oxetane has changed significantly due to (1) the introduction of large substituents in the ring or (2) the addition of either proton or BF3. This structural change is attributed to electrostatic interactions and/or steric repulsions. The nucleophilicity and basicity of oxetane derivatives can be explained by the negative charge and the minimum electrostatic potential value of O atom. The reactivity in the polymerization can be rationalized by (1) the basicity of O atom and (2) the difference between HOMO energy of oxetanes and LUMO energy of activated oxetane polymeric chains. Our calculations predict that 3-azidomethyl-3-methyl oxetane (AMMO) is more basic than 3-nitratomethyl-3-methyl oxetane (NMMO), and AMMO is more reactive toward both AMMO and NMMO polymeric chains. Our results are in good agreement with previous experimental data.