• Title/Summary/Keyword: Molecular Characteristics

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Seven New Recorded Species in Five Genera of the Strophariaceae in Korea

  • Cho, Hae Jin;Lee, Hyun;Park, Jae Young;Park, Myung Soo;Kim, Nam Kyu;Eimes, John A.;Kim, Changmu;Han, Sang-Kuk;Lim, Young Woon
    • Mycobiology
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    • v.44 no.3
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    • pp.137-145
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    • 2016
  • Most known species in the Strophariaceae are decomposers and grow on various kind of organic matter. Approximately 18 genera and 1,316 species in the Strophariaceae have been reported worldwide. Through an ongoing survey of indigenous fungi in Korea, 29 specimens belonging to the Strophariaceae were collected from 2012 to 2016. These specimens were identified based on morphological characteristics and molecular analysis of internal transcribed spacer sequences. Fifteen taxa were confirmed, with eight species matching those previously recorded. Seven species in five genera were shown to be new records in Korea: Galerina marginata, Gymnopilus crociphyllus, Gymnopilus picreus, Hebeloma birrus, Hebeloma cavipes, Pholiota multicingulata, and Psilocybe thaizapoteca. In this study, we provide detailed morphological descriptions of these species and investigate their evolutionary relationships by constructing phylogenetic trees.

Five Species of Endophytic Fungi Isolated from Roots of Native Orchid Plants from Korea (한국 자생 난초 뿌리에서 분리한 미기록 내생균 5종)

  • Lee, Bong-Hyung;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.45 no.4
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    • pp.355-361
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    • 2017
  • In this study, endophytic fungi were isolated from the roots of three native orchid species from Korea: Bletilla striata, Oreorchis patens, and Cephalanthera longibracteata. The isolated fungal endophytes were identified based on the morphological and molecular characteristics including sequence analysis of internal transcribed spacer (ITS) and 28S ribosomal DNA regions. As a result, we discovered 5 species of fungal endophytes that have not been previously reported in Korea: Phialocephala bamuru, Coniochaeta mutabilis, Phialophora foetens, Calonectria canadensis, and Neonectria ramulariae.

An InGaAs/InAlAs multi-quantum well (MQW) avalanche photodiode (APD) with a spacer layer showing low dark current and high speed (고속 광통신 시스템을 위한 다중양자우물구조의 애벌런치 광다이오드의 설계 및 제작)

  • ;;D.L.Sivco;A.Y.Cho;J.M.M.Rios
    • Korean Journal of Optics and Photonics
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    • v.7 no.4
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    • pp.440-444
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    • 1996
  • In this paper, we report an InGaAs/InAlAs multi-quantum well (MQW) avalanche photodiode (APD) showing a performance suitable for 10 Gbps lightwave communications. In designing the device, emphasis is given on the effect of indiffusion of Be dopant from the highly doped field layer into the MQW multiplication region. It is found that a small amount of diffusion can alter the dark current and gain characteristics of the device significantly. A spacer used to restrain such indiffusion is shown effective in reducing dark current (500 nA at a gain of 10) while maintaining a high bandwidth (10 GHz at a gain of 10) devices grown by molecular beam epitaxy.

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Skeletal myogenic differentiation of human periodontal ligament stromal cells isolated from orthodontically extracted premolars

  • Song, Minjung;Kim, Hana;Choi, Yoonjeong;Kim, Kyungho;Chung, Chooryung
    • The korean journal of orthodontics
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    • v.42 no.5
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    • pp.249-254
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    • 2012
  • Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2'-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.

BmKn-2 Scorpion Venom Peptide for Killing Oral Cancer Cells by Apoptosis

  • Tong-ngam, Pirut;Roytrakul, Sittiruk;Sritanaudomchai, Hathaitip
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.7
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    • pp.2807-2811
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    • 2015
  • Scorpion venom peptides recently have attracted attention as alternative chemotherapeutic agents that may overcome the limitations of current drugs, providing specific cytotoxicity for cancer cells with an ability to bypass multidrug-resistance mechanisms, additive effects in combination therapy and safety. In the present study, BmKn-2 scorpion venom peptide and its derivatives were chosen for assessment of anticancer activities. BmKn-2 was identified as the most effective against human oral squamous cells carcinoma cell line (HSC-4) by screening assays with an $IC_{50}$ value of $29{\mu}g/ml$. The BmKn-2 peptide killed HSC-4 cells through induction of apoptosis, as confirmed by phase contrast microscopy and RT-PCR techniques. Typical morphological features of apoptosis including cell shrinkage and rounding characteristics were observed in treated HSC-4 cells. The results were further confirmed by increased expression of pro-apoptotic genes such as caspase-3, -7, and -9 but decrease mRNA level of anti-apoptotic BCL-2 in BmKn-2 treated cells, as determined by RT-PCR assay. In summary, the BmKn-2 scorpion venom peptide demonstrates specific membrane binding, growth inhibition and apoptogenic activity against human oral cancer cells.

A Study on Natural Dyeing using Caesalpinia sappan -Mordanting Effect of Rice Straw Ash Solution- (소목 천연 염색에 관한 연구 II -잿물의 매염효과에 대하여-)

  • Kwon Min-Soo;Choi In-Ryu;Kim Jong-Jun;Jeon Dong-Won
    • The Research Journal of the Costume Culture
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    • v.12 no.6 s.53
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    • pp.908-917
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    • 2004
  • In the previous article, the effect of aluminum compounds on the mordanting of the Caesalpinia sappan L. dyeing was studied. According to the type of the compound, the effect on the dyeing was found to be different even if $Al^{3+}$ ion was present in the molecular structure of the compound. It was postulated that there would be complex effects other than the effects based on the purified Al compounds only, since there are some amount of compounds hard to neglect. In order to reproduce the traditional dyeing practice, rice straw ash solutions were prepared as mordanting agents. The pH values of the solution baths were 4, 5, 6, 9, and 11 in order to investigate the pH effect on the color development by adding appropriate amount of Schizandra chinensis extract solution. The inherent dyeing properties were evaluated by the analysis of color difference, air permeability, the characteristics developed by pre-mordanting, post-mordanting, or simultaneous mordanting methods.

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Experimental Study on HCCI Combustion Characteristics of n-heptane and iso-octane Fuel/air Mixture by using a Rapid Compression Machine (급속압축장치를 이용한 노말헵탄.이소옥탄 혼합연료의 HCCI 연소특성에 대한 연구)

  • Lim, Ock-Taeck
    • Journal of ILASS-Korea
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    • v.16 no.4
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    • pp.167-175
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    • 2011
  • The HCCI engines have been known with high efficiency and low pollution and can be actualized as the new internal combustion engines. However, As for(??) the ignition and combustion depend strongly on the oxidation reaction of the fuel, so it is difficult to control auto-ignition timing and combustion duration. Purpose of this paper is creating the database for development of multi-dimensional simulation and investigating the influence of different molecular structure. In this research, the effect of n-heptane mole ratio in fuel (XnH) on the ignition delay from homogeneous charge compression ignition(HCCI) has been investigated experimentally. By varying the XnH, it was possible to ascertain whether or not XnH is the main resource of ignition delay. Additionally, the information on equivalence ratio for varying XnH was obtained. The tests were performed on a RCM (Rapid Compression Machine) fueled with n-heptane and iso-octane. The results showed that decreasing XnH (100, 30, 20, 10,0), the ignition delays of low temperature reaction (tL) and high temperature reaction (tH) is longer. And the temperature of reaction increases by about 30K. n-heptane partial equivalence ratio (fnH) affect on tL.and TL. When ${\phi}$nH was increased as a certain value, tL was decreased and TL was increased.

Dynamics of OH Production in the Reaction of O(1D2) with Cyclopropane

  • Jang, Sungwoo;Jin, Sung Il;Kim, Hong Lae;Kim, Hyung Min;Park, Chan Ryang
    • Bulletin of the Korean Chemical Society
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    • v.35 no.6
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    • pp.1706-1712
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    • 2014
  • The OH($X^2{\Pi}$, ${\upsilon}^{\prime\prime}=0,1$) internal state distribution following the reaction of electronically excited oxygen atom ($O(^1D_2)$) with cyclo-$C_3H_6$ has been measured using laser-induced fluorescence, and compared with that following the reaction of $O(^1D_2)$ with $C_3H_8$. The overall characteristics of the OH internal energy distributions for both reactions were qualitatively similar. The population propensity of the ${\Pi}(A^{\prime})$ ${\Lambda}$-doublet sub-level suggested that both reactions proceeded via an insertion/elimination mechanism. Bimodal rotational population distributions supported the existence of two parallel mechanisms for OH production, i.e., statistical insertion and nonstatistical insertion. However, detailed analysis revealed that, despite the higher exoergicity of the reaction, the rotational distribution of the OH following the reaction of $O(^1D_2)$ with $C_3H_8$ was significantly cooler than that with cyclo-$C_3H_6$, especially in the vibrational ground state. This observation was interpreted as the effect of the flexibility of the insertion complex and faster intramolecular vibrational relaxation (IVR).

Studies on the Fractional Precipitation of Dextran by Nonsolvent Addition Method (비용매 첨가법을 이용한 Dextran 분별침전에 관한 연구)

  • 최성우;구윤모
    • KSBB Journal
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    • v.10 no.3
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    • pp.257-263
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    • 1995
  • Fractional precipitation of "native" dextran by the method of nonsolvent addition was studied. Precipitational phenomenon of fractioned dextran was characterized with the quantitative determination of the precipitational capacity of nonsolvents with different dielectric constants. Based upon this characterization, the fractional precipitation process for the dextran production was developed. From the precipitational characteristics, a first-order relationship between the molecular weight of fractioned dextran and the content of nonsolvents was formulated. The slopes of the first-order equation were correlated with the dielectric constants of the nonslovents. A modified fractionation process was constructed on the basis of refractionation method and employed to increase the efficiency of the controlled production of dextran.

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Partial Purification and Characteristics of Amylases from Herpetosiphon geysericola (Herpetosiphon geysericola 균주의 Amylase 부분정제 및 특성)

  • Jun, Yeong-Soo;Hong, Yong-Ki;Seu, Jung-Hwn
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.2
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    • pp.128-135
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    • 1987
  • Extracellular ${\alpha}-amylase$, ${\beta}-amylase$ and glucoamylase produced by a thermophilic and cellulolytic bacterium, Herpetosiphon geysericola CUM 317, were partially purified by salting out with ammonium sulfate and by chromatography on a DEAE-cellulose column and on a CM-cellulose column. The Km values of ${\alpha}-amylase$, ${\beta}-amylase$ and glucoamylase for potato starch were $2.31mg/m{\ell}$, $7.69mg/m{\ell}$, and $8.33mg/m{\ell}$. The molecular weights of ${\alpha}-amylase$, ${\beta}-amylase$ and glucoamylase were calculated to be about 84000 dalton, 76000 dalton and 80000 dalton, respectively.

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