• Title/Summary/Keyword: Molecular Characteristics

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Characterization of Potato Scab Pathogens (Streptomyces Species) in Korea

  • Park, Duck-Hwan;Shrestha, Rosemary;Hur, Jang-Hyun;Lim, Chun-Keun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2005.05a
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    • pp.162-165
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    • 2005
  • Potato scab, an important disease that affects developing tubers, causes a major problem in potato cultivation. The major potato cultivation areas in Korea are located in two Northern provinces, Gangwon and Gyeonggi, and two Southern provinces, Jeju island, and South Jeolla. In these areas, potato scab is widely distributed and has caused severe problem in potato cultivation. Therefore, potato-growing areas were surveyed for identification and distribution of potato scab pathogens from 1996 to 1999. Pathogenic Streptomyces strains were isolated from potato scab lesions and six representative Streptomyces species were characterized based on their phenotypic and molecular characteristics including, pathogenicity, physiological and morphological properties, analyses of 16SrRNA genes and 16S-23S ITS region, DNA relatedness, production of thaxtomin A, and the presence of nec1 and ORFtnp gene homologs. Three species were identified as previously described Streptomyces scabies, S. turgidiscabies, and S. acidiscabies, while other three species having distinct phenotypics properties were identified as novel S. luridiscabiei, S. puniciscabiei, and S. niveiscabiei.

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Isolation and Characterization of Four Unrecorded Mucor Species in Korea

  • Nguyen, Thuong T.T.;Jeon, Yu Jeong;Mun, Hye Yeon;Goh, Jaeduk;Chung, Namil;Lee, Hyang Burm
    • Mycobiology
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    • v.48 no.1
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    • pp.29-36
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    • 2020
  • During an investigation of fungi of the order Mucorales from freshwater and sediment samples in Korea, we isolated six strains, NNIBRFG6649, NNIBRFG6255, NNIBRFG1498, CNUFC-YJ13, CNUFC-YR7, and NNIBRFG2739. The morphology and phylogeny of these strains were analyzed. Based on the morphological characteristics and molecular data from internal transcribed spacer (ITS) region, the isolates NNIBRFG6649 and NNIBRFG6255 were identified as Mucor abundans, and M. aligarensis, respectively. The isolates NNIBRFG1498 and CNUFC-YJ13 were identified as M. moelleri, whereas the isolates CNUFC-YR7 and NNIBRFG2739 were identified as M. heterogamus. To the best of our knowledge, M. abundans, M. aligarensis, M. moelleri, and M. heterogamus have not yet been reported in Korea.

Effect of Uniaxial Drawing Conditions on the Orientation of Poly (ethylene 2,6- naphthalate) (일축 연신 조건에 따른 Poly(ethylene 2,6-naphthalate) 배향에 관한 연구)

  • 진병석;이성효;이광희
    • Polymer(Korea)
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    • v.25 no.5
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    • pp.699-706
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    • 2001
  • The effects of uniaxial drawing conditions on the molecular orientation of poly (ethylene 2,6-naphthalate) (PEN) are investigated. Birefringence measurements show that the orientation is significantly enhanced at high draw ratio, low drawing temperature, and fast drawing speed. The characteristics of orientation examined by FTIR- ATR dichroism method represent almost same results. Amorphous orientation function increases with drawing rate at $120^{\circ}C$, but it decreases with drawing rate at $141^{\circ}C$. These behaviors can be explained with the relation between crystallization and chain relaxation rates. It is observed that the orientation of PEN film is accompanied by significant alignment of the naphthalene rings of PEN parallel to the film surface.

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Effect of Polymerization Conditions on the Characteristics of Polyvinyl Acetate Emulsions

  • Youn, Hye-Jung;Lee, Hak-Lae
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.30 no.4
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    • pp.28-34
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    • 1998
  • Polyvinyl acetate emulsion has been widely used as adhesives for wood and paper, paint additives and binders for fiber, leather, and other materials because it is an excellent adhesive with many advantages including low in toxicity risks and manufacturing cost. It is expected the consumption of polyvinyl acetate emulsion as adhesives will increase in cigarette industry as well as in paperboard coating industry. Recently the operation speed of the cigarette tip wrapper increased so substantially that improvement of the emulsion properties is required including good wet tack development, narrow and controlled particle size distribution, low viscosity, etc. In this study the effects of such polymerization conditions as the type and amount of emulsifier, internal or external plasticizing, and emulsification methods on the viscosity and particle size of polyvinyl acetate emulsions were examined. Results showed that polyvinyl alcohol with a high degree of hydrolysis and low molecular weight and nonionic surfactants are superior to anionic surfactant in improving adhesion and emulsion stability. They also tend to produce emulsions with smaller particle size. External plasticization with dipropylene glycol dibenzoate was more effective in improving flexibility than internal plasticization with butyl acrylate. Monomer emulsification under high shear was more effective in decreasing the particle size.

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The Electrical Properties of Film due to the Mixture Ratio of Linear Lour Density Polyethylene and Ethylene Vinyl Acetate (선형 저밀도 폴리에틸렌과 에틸렌 비닐아세테이트의 혼합비에 따른 박막의 전기적 특성)

  • 이충호;박찬원
    • Journal of the Korean Society of Safety
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    • v.14 no.2
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    • pp.83-89
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    • 1999
  • The electrical properties due to mixture ratio of linear low density polyethylene(LLDPE) and ethylene vinyl acetate(EVA) films are studied. An experimental specimen is selected as LLDPE/EVA of thickness 200${\mu}{\textrm}{m}$ produced by mixture ratio of 50 : 50, 60 : 40, 70 : 30 and 80 : 2 wt%. In temperature range from $25^{\circ}C$ to 12$0^{\circ}C$, the measurement of volume resistivity using a highmegohm meter is performed within 10 minutes since each voltage of DC 100 V, 250 V, 500 V and 1000 V is applied, according to the step voltage method. From FT-IR spectrum for an analysis of physical properties, it can be confirmed that LLDPE blended with EVA shows an absence of carbonyl groups(1735 $cm^{-1}$, C=0) and ether groups(1242 $cm^{-1}$, C-O). The peak of LLDPE and EVA made of mixture ratio of 70 : 30 at 2$\theta$ =21.4$^{\circ}$ in the results of XRD is higher than the others. In the experiment for volume resistivity characteristics in order to investigate the electrical properties of specimen, it is confirmed that volume resistivity is decreased with the increase of the molecular motion and temperature.

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Characteristics of Protease Inhibitor Purified from the Eggs of Alaska pollock (Theragra chalcogramma) (명란 단백분해효소 저해제의 특성)

  • STADI U;KIM Keun Yeong;KIM Sang Moo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.2
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    • pp.83-88
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    • 2005
  • Protease inhibitors were purified from the eggs of Alaska pollock (Theragra chalcogramma) using the following purification steps: ammonium sulfate precipitation, ion exchange, gel permeation, and high performance liquid chromatographies (HPLC). The protease inhibitor from the heated eggs of Alaska pollock was not as well purified. In addition, the heated eggs showed lower specific inhibitory activity than the unheated eggs. The purification yields after ammonium sulfate precipitation, ion exchange, and gel permeation chromatographies were 22.7$\%,\;15.3\%$,and $4.4\%$, respectively. There were two kinds of protease inhibitors on the gel permeation chromatography pattern Their molecular weights were estimated to be 66,700 and 16,000 Da, respectively. Both were classified as a cysteine protease inhibitor because of the existence of inhibiting papain, which is one of cysteine proteases.

Roles of Bile Acid as an Active Biological Substance (담즙산의 생체 활성 물질로서의 역할)

  • Bang, Joon-Seok;Lee, Yu-Jeung;Jeong, Ji-Hoon;Sohn, Uy-Dong
    • Korean Journal of Clinical Pharmacy
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    • v.21 no.2
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    • pp.49-56
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    • 2011
  • The family of bile acids belongs to a group of molecular species of acidic steroids with very peculiar biological characteristics. They are synthesized by the liver from cholesterol through several complementary pathways and secreted into small intestine for the participation in the digestion and absorption of fat. The bile acids are mostly confined to the territories of the so-called enterohepatic circulation, which includes the liver, the biliary tree, the intestine and the portal blood with which bile acids are returned to the liver. In patients with bile acid malabsorption, the amount of primary bile acids in the colon is increased compared to healthy controls. Although the increase in the secondary bile acids including deoxycholic acid, is reported to have the potency to affect tumorigenesis in gastrointestinal tracts, there is no firm evidence that clinically relevant concentrations of the bile acids induce cancer. The list of their physiological roles, as well as that of the pathological processes is long and still not complete. There is no doubt that many new concepts, pharmaceutical tools and pharmacological uses of bile acids and their derivatives will emerge in the near future.

Analytic study on Rhizome by Gilles Deleuze : Focusing on the Film (들뢰즈의 리좀 모델 분석 : 영화 <엘리펀트>를 중심으로)

  • Lim, Taewoo
    • The Journal of the Korea Contents Association
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    • v.14 no.1
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    • pp.66-75
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    • 2014
  • The purpose of this study is to analyze and illustrate 'Rhizome', a postmodern speculation system based on de-subjectivity and decentralization, presented by Gilles Deleuze. He borrowed the word rhizome, an originally botanical term meaning a horizontal stem under the surface of the earth, to counteract the dominating system grounded on binary, root-tree structure. The study then criticizes the early movements of de-subjectivity with molecular-biological and evolutionary evidences, and proves that these evidences can only be characterized by chance and nomadism, which are the key elements that constitute Rhizome. As a practical example of Rhizome, the study analyzes the film by Gus Van Sant, and demonstrates that both his various cinematic experiments and the result bear the same characteristics as Rhizome.

Synthesis and Photosensitive Properties of Poly[N-(formyloxyphenyl)maleimide] Containing Photosensitive Groups (Poly[N-(formyloxyphenyl)maleimide] 고분자의 합성과 자외선에 대한 반응특성)

  • Kim, Sang-Min
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.10 no.1
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    • pp.55-62
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    • 2004
  • Synthesis of poly[N-(formyloxyphenyl)maleimide](PFOMI) as photopolymer were investigated with various kinds of photosensitive groups. Generally, photopolyimide have some deficiencies in solubility, sensitivity, reserve stability of the photosensitive solution, and the precision of image pattern. The study has been required on those polymers which have high glass transition temperature and photo efficiency, and low dielectricity. The existing condensation resins require high curing temperature and perfect elimination of subreacted materials that are produced during the process after irradiation and various membrane damages such as the deformation and contraction in image pattern cure. In this study poly[N-(hydroxyphenyl)maleimide](PHPMI) was synthesized. The PHPMI were analyzed by H-NMR and FT-IR. The measured number average molecular weight of PHPMI was produced was $1.06{\times}10^4$. Poly[N-(formyloxyphenyl)maleimide](PFOMI) as a type of photo-Fries rearrangement was synthesized by NHPMI and formic acid followed by radical polymerization. PFOMI was analyzed by FT-IR, and photocharacteristics was investgated by UV spectra and FT-IR before and after UV irradiation. Based on the image characteristics of PFOMI measured from optical micrographs, it was formed that the resolution of positive type PFOMI was $0.5{\mu}m$.

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Investigation of the Nature of the Endogenous Glucose Transporter(s) in Insect Cells

  • Lee, Chong-Kee
    • BMB Reports
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    • v.32 no.5
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    • pp.429-435
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    • 1999
  • Unlike the mammalian glucose transporter GLUT1, little is known about the nature of the endogenous sugar transporter(s) in insect cells. In order to establish the transport characteristics and other properties of the sugar transport proteins of Sf9 cells, a series of kinetic analyses was performed. A saturable transport system for hexose uptake has been revealed in the insect cells. The apparent affinity of this transport system(s) for 2-deoxy-D-glucose was relatively high, the $K_m$ for uptake being <0.5 mM. To further investigate the substrate and inhibitor recognition properties of the insect cell transporter, the ability of other sugars or drugs to inhibit 2-deoxy-D-glucose transport was examined by measuring inhibition constants ($K_j$). Transport was inhibited by D-mannose, D-glucose, and D-fructose. However, the apparent affinity of the C-4 epimer, D-galactose, for the Spodoptera transporter was relatively low, implying that the hydroxyl group at the C-4 position may play a role in the strong binding of glucose and mannose to the transporter. The results also showed that transport was stereoselective, being inhibited by D-glucose but not by L-glucose. It is therefore concluded that insect cells contain an endogenous glucose transport activity that in several aspects resembles the human erythrocyte glucose transporter. However, the mammalian and insect transporters were different in some of their kinetic properties, namely, their affinities for fructose and for cytochalasin B.

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