• Title/Summary/Keyword: Molecular Characteristics

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Effects of Calcium Chloride Concentration and Reaction Time on Physical and Sensory Characteristics of Persimmon Calcium Alginate Beads (염화칼슘 농도와 반응시간에 따른 감 칼슘 알지네이트 비드의 물리적 및 관능적 특성)

  • Yong, Dong-Hee;Song, Min-Kyung;Yoon, Hye-Hyun
    • Culinary science and hospitality research
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    • v.18 no.4
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    • pp.209-221
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    • 2012
  • The purpose of this study is to apply molecular gastronomy and its spherification methodology to persimmon desserts. We prepared persimmon calcium alginate beads and investigated physical and sensory characteristics of beads according to the different concentration of calcium chloride(0.5, 0.75, 1.0%) and reaction time(2, 4, 6, 8, 10 min). Lightness and yellowness were decreased significantly as both calcium chloride concentration and reaction time increased. However, redness was increased significantly as the concentration of calcium chloride and reaction time increased. Hardness, springiness, chewiness, cohesiveness, and resilience except for adhesiveness tended to increase as the concentration of calcium chloride and reaction time increased. The thickness of beads also increased as the concentration of calcium chloride and reaction time increased. Quantitative descriptive analysis showed that voluminosity, springiness, hardness, chewiness, and residue tended to increase as the concentration of calcium chloride increased. Overall acceptability reached a peak at the persimmon bead containing 0.5% calcium chloride. The result of this study showed that the concentration of calcium chloride and reaction time influenced the overall characteristics of calcium alginate beads.

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Effect of Ginseng Powder on Quality Characteristics of Instant Rice Cake (Baekseolgi) (즉석 백설기 제조시 인삼분말 첨가가 백설기의 품질에 미치는 영향)

  • Kang, Ho-Jin;Kim, Seung-Hee;Kum, Jun-Seok;Lim, Jae-Kag
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.3
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    • pp.435-442
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    • 2010
  • This study was to investigate the quality characteristics of instant rice cake (Baekseolgi) with different concentrations of ginseng powder by molecular press dehydration (MPD) method. Baekseolgi was cooked using microwave oven, which is a useful cooking method because of reduced cooking time, even though it quickly takes moisture from food surface and lowers food quality. The result indicated that moisture content of Baekseolgi without ginseng powder was decreased; however, there was no significant difference in the Baekseolgi with ginseng powder with increased storage time for 24 hrs. The L value was decreased and a color value was increased with increasing concentrations of ginseng powder (p<0.05). There was no significant difference in the color values within the storage time. The hardness, gumminess, and chewiness of Baekseolgi without ginseng powder significantly changed as the storage time increased; in contrast, those of Baekseolgi with ginseng powder did not show such a significant change. The result of sensory evaluation showed that Baekseolgi with ginseng powder has higher scores in terms of color, flavor, taste, and overall acceptability than Baekseolgi without ginseng powder. Results of this study indicate the addition of ginseng powder to Baekseolgi improves moisture content, color value, and texture properties as well as sensory characteristics.

The Influence of Land Use on the Concentration Levels and Distribution Characteristics of Polycyclic Aromatic Hydrocarbons (PAHs) in Korea (국내 토지이용도별 토양 중 다환방향족탄화수소류(PAHs)의 농도 수준 및 분포 특성)

  • Noh, Hoe-Jung;Yoon, Jeong Ki;Yun, Dae-Geun;Yu, Soon-Ju;Kim, Tae Seung;Lee, Jai-Young
    • Journal of Soil and Groundwater Environment
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    • v.19 no.6
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    • pp.59-71
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    • 2014
  • We investigated soil contamination depending on the land use by examining the contamination levels and distribution characteristics of 16 polycyclic aromatic hydrocarbons (PAHs) in the national soil. Total PAHs (the sum of 16 PAH concentrations) and carcinogenic PAHs (the sum of seven carcinogenic PAH concentrations) were $8.50{\sim}3,437.16{\mu}g/kg$ and $2.94{\sim}2,136.96{\mu}g/kg$, respectively. The concentration of benzo(a)pyrene, one of the contaminants regulated by the soil quality standard in the nation, was $ND{\sim}924.73{\mu}g/kg$. Its maximum value of $924.73{\mu}g/kg$ was detected in railroad (Region 3) and is approximately 13% of the standard value for Region 3 (i.e., 7 mg/kg). We also investigated the characteristics of contamination sources of PAHs in soil of the upland, forests, roads, and railroads, examining the fraction distribution of PAHs concentration by the number of benzene rings against the total PAHs concentration. The results demonstrate that the mean fraction of 4~6-ring PAHs against total PAHs concentration in soil was in the range of 51.8~80.7% with relative abundance of high-molecular PAHs, showing that the origin of contamination is under the category of combustion sources. When the molecular indices (Flu/(Flu/Pyr), Ant/(Ant+Phe), InP/(InP+BP), and BaA/(BaA+Chr)) were applied, they were also categorized as petroleum-based combustion sources. The individual PAH concentrations in soil by the land use were grouped into Regions 1, 2, and 3, which are statistically treated and are the parts of the national category system of soil quality standard. As a result, the concentration level of 16 PAHs was $0.02{\sim}2.63{\mu}g/kg$ in Region 1, $0.05{\sim}4.26{\mu}g/kg$ in Region 2, and $2.36{\sim}178.27{\mu}g/kg$ in Region 3. The concentration level of 6 carcinogenic PAHs was 14.2~320.4% against that of benzo(a)pyrene in Region 3 and sites of recycling waste sleepers. Considering that there were similarities among PAHs in terms of structures and toxicities, it would be recommended to review other types of carcinogenic PAHs together with benzo(a)pyrene when developing the soil quality standards in the nation.

Structural Characteristics and Properties of Silk Fibroin/Polyurethane Blend Films

  • Um, In-Chul;Kweon, Hae-Yong;Chang mo Hwang;Min, Byung-Goo;Park, Young-Hwan
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.2
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    • pp.163-170
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    • 2002
  • In this paper, silk fibroin (SF)/polyurethane (PU) blend films were fabricated to develop a new biomaterial for biomedical applications. These blend films were prepared using formic acid as a cosolvent, and structural characteristics and properties of blend films were investigated. FTIR results suggested that there was no specific interaction between SF and PU, implying molecular immiscibility in SF/PU blend films. Furthermore, it was revealed by XRD method that the crystalline region of blend components was not perturbed by counterpart polymers. The degree of phase separation of SF/PU blend films was diminished by increasing PU content in blend. Especially, the blend with 70% content of PU showed no evidence of macro-phase separation in SEM observation. However, SF/PU blend (70/30) was revealed to be phase-separated in a lower dimension confirmed by DMTA measurement. TGA result showed that thermal decomposition temperature of blend film was slightly decreased compared to those of SF and PU polymer itself, Though mechanical properties of SF/PU blend films were not good enough due to the solvent, blood compatibility of PU can be enhanced markedly by mixing with SF for SF/PU blend film.

Purification and Characteristics of Glucoamylase in Aspergillus oryzae NR 3-6 Isolated from Traditional Korean Nuruk

  • Yu, Tae-Shick;Kim, Tae-Hyoung;Joo, Chong-Yoon
    • Journal of Microbiology
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    • v.37 no.2
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    • pp.80-85
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    • 1999
  • The purification system of glucoamylase (glucan 1,4-${\alpha}$-glucosidase, EC 3. 2. 1. 3), some characteristics of the purified enzyme and hydrolysis rate of various raw starch were investigated through several experiments. The enzyme was produced on a solid, uncooked wheat bran medium of Aspergillus oryzae NR 3-6 isolated from traditional Korean Nuruk. The enzyme was homogeneously purified 6.8-fold with an overall yield of 28.3% by the criteria of disc- and SDS-polyacrylamide gel electrophoresis. The molecular weight was estimated to be 48 kDa by SDS-PAGE. The optimum temperature and pH were 55$^{\circ}C$ and 4.0, respectively. The enzyme was stable at a pH range of 3.0∼10.0 and below 45$^{\circ}C$. Enzyme activity was inhibited about 27% by 1mM Hg2+. The hydrolysis rate of raw wheat starch was shown to be 17.5-fold faster than the hydrolysis rate of soluble starch. The purified enzyme was identified as glucoamylase because the product of soluble starch by the purified enzyme was mainly glucose by thin layer chromatography.

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A Study on Development of Protein Materials using Dead Flatfish from Fish Farms(1) -Antioxidant Functional Properties- (양식장 넙치 폐사어를 이용한 단백질 소재의 개발에 관한 연구(1) -항산화 기능특성-)

  • Kang, Keon-Hee;Lee, Min-Gyu;Kam, Sang-Kyu;Jeong, Kap-Seop
    • Journal of Environmental Science International
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    • v.22 no.11
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    • pp.1465-1471
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    • 2013
  • To develop the protein materials by the reutilization of dead flatfish from fish farms in Jeju island, the physicochemical characteristics and the functional activities of collagen peptide extracts were investigated. Flatfish skin collagen peptide (FSCP) and flatfish protein hydrolysate (FPH) were manufactured from dead flatfish. The differences of pH, moisture and fat contents between FSCP and FPH were not significant, fat contents were analyzed less than 0.3%, and trans-fat, saturated fat and cholesterol were not detected in both samples. Protein contents of FSCP and FPH showed about 92% and 95%, respectively. In the analysis of amino acids, glycine and hydroxy proline content in FSCP was 24.22% and 6.15%, respectively, showed a typical characteristics of the collagen protein, but essential amino acids contents such as threonine, valine, methionine, isoleusine, leusine and phenylalanine were relatively higher than those of FPH. Average molecular weight of FSCP was measured as 1,102 which was almost equal value with that of tuna collagen peptide. The antioxidant activities and functional properties showed high but did not show significant difference between two samples.

Calcium-binding Peptides Derived from Tryptic Hydrolysates of Cheese Whey Protein

  • Kim, S.B.;Lim, J.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.10
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    • pp.1459-1464
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    • 2004
  • The purpose of this research was to investigate the potential use of cheese whey protein (CWP), a cheese by-product. The physiological activity of calcium-binding peptides in CWP may be used as a food additive that prevents bone disorders. This research also examined the characteristics of calcium-binding peptides. After the CWP was heat treated, it was hydrolyzed by trypsin. Then calcium-binding peptides were separated and purified by ion-exchange chromatography and reverse phase HPLC, respectively. To examine the characteristics of the purified calcium-binding peptides, amino acid composition and amino acid sequence were analyzed. Calcium-binding peptides with a small molecular weight of about 1.4 to 3.4 kDa were identified in the fraction that was flowed out from 0.25 M NaCl step gradient by ion-exchange chromatography of tryptic hydrolysates. The results of the amino acid analysis revealed that glutamic acid in a calcium-binding site took up most part of the amino acids including a quantity of proline, leucine and lysine. The amino acid sequence of calcium-binding peptides showed Phe-Leu-Asp-Asp-Asp-Leu-Thr-Asp and Ile-Leu-Asp-Lys from $\alpha$-LA and Ile-Pro-Ala-Val-Phe-Lys and Val-Tyr-Val-Glu-Glu-Leu-Lys from ${\beta}$-LG.

Prognostic Value of Caveolin-1 Expression in Gastric Cancer: a Meta-analysis

  • Ye, Yang;Miao, Shu-Han;Lu, Rong-Zhu;Zhou, Jian-Wei
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.19
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    • pp.8367-8370
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    • 2014
  • The relationship between caveolin-1 (Cav-1) and clinicopathological characteristics of gastric cancer is controversial, although Cav-1 plays an important role in tumor metastasis. To evaluate the clinicopathological and prognostic value of expression in patients with gastric cancer, a meta-analysis was performed to investigate the impact on clinicopathological parameters and prognosis in gastric cancer cases. Studies assessing these parameters for Cav-1 in gastric cancer were identified up to June 2014. Finally, a total of six studies met the inclusion criteria. Our combined results showed that Cav-1 expression was significantly associated with the Lauren classification (pooled OR=0.603, 95% CI: 0.381-0.953, P=0.030). Furthermore, we found that Cav-1 expression predicted a better overall survival in gastric cancer patients (pooled OR=0.590, 95% CI: 0.360-0.970, P=0.038, fixed-effect). In conclusion, the overall data of the present meta analysis showed that Cav-1 expression was not correlated with clinicopathological features except for the Lauren classification. Simultaneously, Cav-1 overexpression predicted a better overall survival in gastric cancer. Cav-1 expression in tumors is a candidate positive prognostic biomarker for gastric cancer patients.

Determination of Amino Acids on Wintertime PM2.5 using HPLC-FLD (HPLC-FLD를 이용한 겨울철 PM2.5 중 아미노산 성분 분석)

  • Park, Da-Jeong;Cho, In-Hwan;Bae, Min-Suk
    • Journal of Korean Society for Atmospheric Environment
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    • v.31 no.5
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    • pp.482-492
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    • 2015
  • Ground-based measurements were conducted from January 6 to 12 of 2015 for understanding characteristics of nitrogen containing carbonaceous aerosols as 16 amino acids at the Mokpo National University, Korea. The detailed amino acid components such as Cystine ($(SCH_2CH(NH_2)CO_2H)_2$) and Methionine ($C_5H_{11}NO_2S$) and their sources were analyzed by High-Performance Liquid Chromatography with Fluorescence Detection (HPLC-FLD) for behavior of secondary products in particulate matter. In addition, organic carbon (OC) and elemental carbon (EC) based on the carbonaceous thermal distribution (CTD), which provides detailed carbon signature characteristics relative to analytical temperature, and water soluble organic carbon (WSOC) by total organic carbon (TOC) analyzer were used to understand the carbon compound behaviors. The backward trajectories were discussed for originations of carbonaceous aerosols as well. Different airmasses were classified with the amino acids and OC thermal signatures. The results can provide to understand the aging process influenced by the long-range transport from East Sea area.

Forward and backward whirling of a spinning nanotube nano-rotor assuming gyroscopic effects

  • Ouakad, Hassen M.;Sedighi, Hamid M.;Al-Qahtani, Hussain M.
    • Advances in nano research
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    • v.8 no.3
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    • pp.245-254
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    • 2020
  • This work examines the fundamental vibrational characteristics of a spinning CNT-based nano-rotor assuming a nonlocal elasticity Euler-Bernoulli beam theory. The rotary inertia, gyroscopic, and rotor mass unbalance effects are all taken into consideration in the beam model. Assuming a nonlocal theory, two coupled 6th-order partial differential equations governing the vibration of the rotating SWCNT are first derived. In order to acquire the natural frequencies and dynamic response of the nano-rotor system, the nonlinear equations of motion are numerically solved. The nano-rotor system frequency spectrum is shown to exhibit two distinct frequencies: one positive and one negative. The positive frequency is known as to represent the forward whirling mode, whereas the negative characterizes the backward mode. First, the results obtained within the framework of this numerical study are compared with few existing data (i.e., molecular dynamics) and showed an overall acceptable agreement. Then, a thorough and detailed parametric study is carried out to study the effect of several parameters on the nano-rotor frequencies such as: the nanotube radius, the input angular velocity and the small scale parameters. It is shown that the vibration characteristics of a spinning SWCNT are significantly influenced when these parameters are changed.