• 한국산업융합학회 논문집
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• 제26권6_2호
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• pp.1011-1017
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• 2023

In this study, the harmless crack size (a_hml) by double shot peening (DSP) using shot balls with different diameters was evaluated on carburized, quenched-tempered SCM822H steel. The minimum crack size (a_NDI) detectable by non-destructive inspection was also evaluated. The relationship between the crack size (a_25,50) that reduces the fatigue limit by 25% and 50% and a_hml was evaluated. The residual stress of DSP was greater in SP_(0.6+0.08) than SP_(0.8+0.08) and appeared deeper in the depth direction. In addition, the hardness below the surface appeared larger. The fatigue limit of DSP increased 2.07 times and 1.95 times compared to non-SP. All a_hml of the DSP specimen was determined at the depth (a). The compressive residual stress distribution affects a_hml, and the a_hml of SP_(0.6+0.08), which has a large compressive residual stress and a high fatigue limit, appeared large. a_hml of SP_(0.6+0.08) introduced deeper than the residual stress of SP_(0.8+0.08) is larger in the range of As=1.0-0.3. Since the residual stress in the thickness direction has a greater effect on a_hml than the residual stress at the surface, it is necessary to introduce it more deeply. The relation of a_hml, a_25,50, and a_NDI were evaluated in the point for safety and reliability.

• Design & Manufacturing
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• 제15권2호
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• pp.11-16
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• 2021

Recently, three-dimensional (3D) cell culture systems, which are superior to conventional two-dimensional (2D) vascular systems that mimic the in vivo environment, are being actively studied to reproduce drug responses and cell differentiation in organisms. Conventional two-dimensional cell culture methods (scaffold-based and non-scaffold-based) have a limited cell growth rate because the culture cannot supply the culture medium as consistently as microvessels. To solve this problem, we would like to propose a 3D culture system with an environment similar to living cells by continuously supplying the culture medium to the bottom of the 3D cell support. The 3D culture system is a structure in which microvascular structures are combined under a scaffold (agar, collagen, etc.) where cells can settle and grow. First, we have manufactured molds for the formation of four types of microvessel-mimicking chips: width / height ①100 ㎛ / 100 ㎛, ②100 ㎛ / 50 ㎛, ③ 150 ㎛ / 100 ㎛, and ④ 200 ㎛ / 100 ㎛. By injection molding, four types of microfluidic chips were made with GPPS (general purpose polystyrene), and a 100㎛-thick PDMS (polydimethylsiloxane) film was attached to the top of each microfluidic chip. As a result of observing the flow of the culture medium in the microchannel, it was confirmed that when the aspect ratio (height/width) of the microchannel is 1.5 or more, the fluid flows from the inlet to the outlet without a backflow phenomenon. In addition, the culture efficiency experiments of colorectal cancer cells (SW490) were performed in a 3D culture system in which PDMS films with different pore diameters (1/25/45 ㎛) were combined on a microfluidic chip. As a result, it was found that the cell growth rate increased up to 1.3 times and the cell death rate decreased by 71% as a result of the 3D culture system having a hole membrane with a diameter of 10 ㎛ or more compared to the conventional commercial. Based on the results of this study, it is possible to expand and build various 3D cell culture systems that can maximize cell culture efficiency by cell type by adjusting the shape of the microchannel, the size of the film hole, and the flow rate of the inlet.

• 한국가스학회지
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• 제4권1호
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• pp.26-32
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• 2000

본 연구에서는 힌지재인 SM20C, YBsC3 및 STC4H재에 대하여 공기 및 여러 가지 부식환경중에서 이종금속간의 찰과마멸부식실험을 실시하여, 이종금속간의 찰과마멸부식특성에 미치는 환경조건의 영향을 연구하였으며, 주요 결론은 다음과 같다. 1) 이동측 금속인 SM20C의 찰과마멸부식에 미치는 지하수의 영향은 STC4H에서 더 민감하고 YBsC3에서는 둔화된다. 2) STC4H의 찰과마멸부식에 미치는 지하수의 영향은 작지만, $0.5\%\;H_2SO_4$ 및 $0.5\%HNO_3$ 용액중에서 더 크게 된다. 3) 이동측 SM20C의 찰과마멸부식은 $3.5\%\;NaCl$, $0.5\%\;H_2SO_4$ 및 $0.5\%\;HNO_3$ 용액중에서보다 지하수중에서 가장 작게 나타났다. 4) 시간이 경과함에 따라 찰과마멸부식에 미치는 영향은 $0.5\%\;HNO_3$ 용액중에서는 증가하지 $0.5\%\;H_2SO_4$ 용액중에서는 둔화된다.