• Title/Summary/Keyword: Mold Analysis

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Effect of LED Irradiation on Growth Characteristids of Ginseng Cultivated in Plastic Film House

  • Seo, Sang Young;Cho, Jong hyeon;Kim, Chang Su;Kim, Hyo Jin;Kim, Dong Won;An, Min Sil;Yoon, Du Hyeon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.45-45
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    • 2019
  • This experiment was carried out using artificial clay and LED in the plastic film house (irradiation time: 08:00~18:00/day). Seedlings (n = 63 per $3.3m^2$) of ginseng was planted on May 17, 2018. LED was combined with red and blue light in a 3:1 ratio and irradiated with different light intensity. The average air temperature from April to September was $12.3^{\circ}C$ $-26.0^{\circ}C$ and it was the the highest at $26.0^{\circ}C$ in August. The test area where fluorescent lamp was irradiated tended to be somewhat higher than the LED irradiation area. The chemical properties of the test soil are as follows. pH levels was 5.3~5.5, EC levels 0.45~0.52 dS/m and OM levels 33~37%. The total nitrogen content was 0.35~0.47% and the available $P_2O_5$ contents was 13.7~16.0 mg/kg, which was lower than the suitable level of 70~200 mg/kg. Exchangeable cations K and Mg contents were within acceptable ranges, but the Ca contents was $28{\sim}38cmol^+/kg$ levels higher than the permissible level ($2{\sim}6cmol^+/kg$). Germination of ginseng leaves took 8~9 days and the overall germination rate was 70~75%. The photometric characteristics of LED light intensity are as follows. The greater the light intensity, the higher the PAR (Photosynthetic Action Radiation) value, illuminance and solar irradiation. Photosynthetic rate was also increased with higher light intensity was investigated at $1.7{\sim}3.2{\mu}mol\;CO_2/m^2/s$. Leaf temperature ($23.7{\sim}24.8^{\circ}C$) by light intensity was the same trend. The growth of aerial parts (plant height etc.) were generally excellent when irradiated with 3 times the light intensity, the growth of the ginseng aerial parts were excellent as follows. The plant height was 42.6 cm, stem length was 25.2 cm, leaf length was 9.6 cm and stem diameter was 5.0 mm. The growth of underground part (root length etc.) was the same, and the root length was 24.4 cm, the tap root length was 6.0 cm, diameter of taproot was 18.2 mm and the fresh root weight was 17.2 g. There were no disease incidence such as Alternaria blight, Gray mold and Anthracnose. Disease of Damping off occurred 2.2~3.6% and incidence ratio of rusty root ginseng was 14.6~20.7%. Leaf discoloration rate was 13.7~48.9% and increased with increasing light intensity. Ginsenoside content of ginseng by light intensity is under analysis.

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Perception Difference Analysis between Manager and Field Worker about the Form work Collapse Accident (거푸집 붕괴재해에 대한 관리자와 작업자의 인식차이 분석)

  • Kang, Sung Won;Lee, Jun Heon;Park, Se Hwan;Kang, Ha Ram;Lee, Ki Seok;Kim, Baek-Joong;Shin, Yoon-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.2
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    • pp.179-187
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    • 2021
  • In the past five years, form construction, which accounts for approximately 25% of the total construction period, has continued to occur without a significant decrease in the number of deaths, with the largest proportion of collapse accidents. Accordingly, this study analyzed the difference in perception between the manager and worker by a questionnaire survey on the degree of risk and safety management level regarding a mold collapse accident. The survey was conducted in three groups: safety manager, field worker, and field manager. The results of the survey were analyzed by an independent sample T-Test using the SPSS program. As a result, there was almost no difference in recognition between managers, but a significant difference in recognition between managers and workers. In addition, there was a difference in management perception between the administrator and worker, which clearly shows the difference in the position between the administrator who manages and supervises hazardous disaster factors and the worker who works directly in the field. Such differences in perception can be a factor that cannot be mitigated. Based on this study, more developed research can narrow the perception gap between managers and workers and be used as a basic material for disaster research.

An Analysis of the Current Status and Characteristics of Airborne Fungi in Indoor Air in Multi-Use Facilities Nationwide (전국 다중이용시설의 실내공기 부유 곰팡이의 현황 및 특성 분석)

  • Park, Yongsung;Kwon, Soonhyun;Park, Song-Yi;Kee, Sun-Ho;Yoon, Wonsuck
    • Journal of Environmental Health Sciences
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    • v.48 no.5
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    • pp.282-289
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    • 2022
  • Background: Airborne fungi are ubiquitous in the air and exposure to an airborne fungus can be a significant risk factor. The composition of fungi has been potentially important for human health, especially for respiratory diseases like asthma and atopic dermatitis. Therefore, we attempted to ascertain what kind of airborne fungi affect human health at a nationwide level. Objectives: This study was carried out to provide information on indoor fungi distribution at multi-use facilities throughout South Korea. Methods: We classified our data by region and public facility after collection, cultivation, and identification via the sequencing of the ITS (internal transcribed spacer) region. We investigated whether or not the proliferation of HaCaT cells was affected by the identified airborne fungi. Results: In our data, the most isolated airborne fungi by region were Penicillium spp (Seoul, Daegu), Periconia sp (Gyeonggi-do), Iprex sp (Gangwon-do), Phanerochaete sp (Busan), Bjerkandera sp (Gwangju), and Aspergillus sp (Jeju-do). In the public facilities, the most detected fungi were Cladosporium sp (public transport), Penicillium sp (apartment house, retail market, financial institution, karaoke room), Bjerokandera sp (underground parking lot, public toilet, medical institution), Periconia sp (retail store), and Fusarium sp (general restaurant). Next, we selected twenty airborne fungi to examine their cytotoxicity and proliferation of human skin cells. In this experiment, the proliferation of the cells was influenced by most of the identified fungi. In case of the cytotoxicity test, most genera except for Rhodotorula sp and Moesziomyces sp showed cytotoxicity in HaCaT cells. Conclusions: The distribution of mold in the indoor air in multi-use facilities in South Korea differs from region to region, and this is an indicator that should be considered in future health impact studies. In addition, as a result of culturing about 20 types of bacteria dominant in indoor air, it was found that most (90%) inhibit the growth of skin cells, which can be harmful to health. An in-depth study of the health effects of floating fungi is needed.

Interpretation of Making Techniques through Surface Characteristic Analysis and Non-destructive Diagnosis for the Gilt-bronze Seated Buddha in Dangjin Sinamsa Temple, Korea (당진 신암사 금동여래좌상의 표면특성 분석과 비파괴 정밀진단을 통한 제작기술 해석)

  • CHOI Ilkyu ;YANG Hyeri ;HAN Duru;LEE Chan Hee
    • Korean Journal of Heritage: History & Science
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    • v.56 no.1
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    • pp.100-116
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    • 2023
  • The Sinamsa Temple was built in the late Goryeo Dynasty and a gilt-bronze seated Buddha is enshrined in Geungnakjeon hall in the precinct. Various damages occurred in the gilt layer of the Buddha, such as peeling of the gilt layer and deteriorating gloss. In the study, the conservation conditions of the inside and outside on the statue were accurately investigated, and the making technique was interpreted through the material characteristics and non-destructive diagnosis of the statue. As a result, it is estimated that gold-gilding layer is pure gold, coloration pigment of black is carbon, green is malachite, atacamite and verdigris, red is red lead and cinnabar, respectively. In the deterioration evaluation, peeling, cracking, break out and exfoliation of the gilt layer are confirmed as damages, but the conservation condition is relatively wholesome. However, the gloss of the gilt layer is calculated to be wider in the poorer part than the maintenance part. The ultrasonic velocity of the statue was calculated to be 1,230 to 3,987 (mean 2,608) m/s and showed a relatively wide range. In infrared thermography, peeling was not confirmed, and no special bonding marks were found. In endoscope, some biological damage and corrosion were observed on the surface of the internal metal, and sealed artifacts were identified. Manufacturing technique based on the study, it is considered that the gilt-bronze seated Buddha was cast at once, and the mold was inverted to inject molten metal.

Structural analysis of Precipitates in a Nickel based Cast Single Crystal of CMSX 6 (니켈계 초합금 CMSX 6 단결정 주조조직의 석출물구조 분석)

  • An, Seong-Uk;Larionov, V.;Grafas, I.;Kim, Su-Cheol;Im, Ok-Dong;Kim, Seung-Ho;Jin, Yeong-Hun;Choe, Jong-Su;Lee, Jae-Hun;Lee, Sang-Jun;Seo, Dong-Lee;Lee, Tae-Hun;Heo, Mu-Yeong
    • Korean Journal of Materials Research
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    • v.8 no.12
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    • pp.1165-1169
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    • 1998
  • A single crystal cast blade was manufactured by CMSX 6, one of the first generarion nickel based single crystal superalloys by the selector method in a vacuum furnace. The single crystal has been grown with cooling rate of 2.5 mm/min, after pouring the molten alloy of 163$0^{\circ}C$ to the mold heated to 150$0^{\circ}C$. The cast structure could be classified into matrix (dendrite) and eutectic regions in ${\gamma}$'shape and size. The eutectic region showed higher Ti content. As the additional results of ${\gamma}$'precipitates by EPMA and CBED analysis the ${\gamma}$'size was less than 0.5~0.7$\mu\textrm{m}$, showing the chemical composition close to Ni$_3$Al of Ll$_2$ lattice structure. But ${\gamma}$'size has increased to bigger than 1.0$\mu\textrm{m}$, being near to eutectic region, changing its shape to bar or huge block types. These showed the chemical structure near to Ni$_3$Ti of D $O_{24}$ lattice structure. Therefore, ${\gamma}$'morphology of dendrite and eutectic regions depends absolutely on its chemical composition and lattice structure.

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Study on the Manufacturing techniques & Conservation of Iron Pot from Cheonmachong Ancient Tomb (천마총 출토 철부(鐵釜)의 제작기법 및 보존처리)

  • Lee, Seung Ryul;Shin, Yong Bi;Jung, Won Seob
    • Journal of Conservation Science
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    • v.30 no.3
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    • pp.263-275
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    • 2014
  • It's shown how to proceed the study on Manufacturing techniques & Conservation to the Iron Pot from Cheonmachong Ancient Tomb(the 155th Tomb in Hwangnam-dong). In order to investigate manufacturing techniques of the Iron Pot, some parts of the relic were gathered. After mounting, polishing and etching on the relic, analyzing the metal microstructure was conducted. Also it's conducted a SEM-EDS analysis on the nonmetallic inclusion. White iron structure was observed in the metallurgical structure inspection, SEM-EDS analysis. It seems to be dried slowly at room temperature after casting, doesn't look as particular heat treatment to improve brittleness. It is estimated that it's as the handle seam side were verified about 3cm inch wide, 1.5 thick in center of body, so 2 separate half-completed products was cast with width-type mould. The manufacturing techniques Using white cast iron structure, width-type mould are observable to the Iron Pot excavated from Sikrichong Ancient Tomb & Hwangnamdaechong grand Ancient Tomb around those were constructed the same time. It's able to recognize that it's almost identical manufacturing techniques at that time. Conservation is generically following those are survey of pretreatment, foreign material removal, stabilization, restoration and color matching in the order. cleaning & drying were added to the process as occasion demands. The strengthening treatment were difficult with artifact's volume, low concentration Paraloid NAD-10 solution was spread two or three times with a brush, surface hardening also came up with 15wt% Paraloid NAD-10 solution after the conservation was complete. There were connection & restoration for the restoration to the damage after modeling forms that it's similar to damaged parts by using the Fiber Reinforced Plastic resins(POLYCOAT FH-245, mold laminated type). Throughout this research, capitalizing on accumulations of measurements about the production technique of Iron Pot in the time of the fifth and 6th centuries is no less important than the Iron artifact's conservation for a better study in the future.

Synthesis of Ultra High Refractive Index Monomer for Plastic Optical Lens and Its Ophthalmic Lens Preparation (플라스틱 안경렌즈용 초고굴절 모노머 합성 및 이를 이용한 안경렌즈 제조)

  • Jang, Dong Gyu;Kim, Jong Hyo;Lee, Soo Min;Roh, Soo Gyun
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.3
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    • pp.1-6
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    • 2008
  • Purpose: Plastic optical monomer materials having ultra high refractive index have an income of the whole quantity from advanced nations to domestic companies which are related to plastic optical lens. It is necessary to develop novel plastic optical lens materials in order to overcome a FTA provision and revitalize a stagnating optical lens industry in the interior optical lens industries. The new plastic optical lens materials against the substitution effect of income should be gradually demanded. This work will be synthesized novel super high refractive monomer resin materials of urethane lens series and studied the properties of optical lens using it. Methods: ETS-4 (2-(2-mercaptoethylthio)-3-{2-[3-mercapto-2-(2-mercaptoethylthio)propyl thio]ethylthio}propane -1-thiol), which is optical lens monomer resin having super high refractive index, was synthesized and identified its structure and property by elemental analysis, EI-MS, TGA, FT-IR spectroscopy, $^1H$ and $^{13}C$ NMR spectroscopies. After mixing evenly from mixed monomer resin and diisocyanate series, it was casting in glass mold. After thermal curing, the obtained optical lenses were measured and compared with the refractive index and Abbe number for studies of their optical properties. Results: We have synthesized the novel ultra high refractive index monomer resin, ETS-4, and have identified its structure and property by elemental analysis, EI-MS, TGA, FT-IR spectroscopy, $^1H$ and $^{13}C$ NMR spectroscopies. The existence of three isomers for EST-4 was identified by $^{13}C$ NMR spectroscopy. The refractive index ($N_d$ at $25^{\circ}C$) of monomer resin in liquid state obtained from the Abbe refractometer was 1.647. The refractive indexes of raw plastic optical lenses prepared from the mixed ETS-4 monomer and diisocyanate series were in the range of 1.656~1.680. Conclusions: Novel super high refractive index plastic optical lens monomer was synthesized and analysed, the optical lenses prepared using it were colorless transparency and excellent properties. It is of utility for the industrialization.

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Effects of specimens dimension on the flexural properties and testing reliability of dental composite resin (치과용 복합레진의 굽힘 특성과 시험 신뢰도에 미치는 시편 크기의 영향)

  • Im, Yong-Woon;Hwang, Seong-sig;Kim, Sa-hak;Lee, Hae-Hyoung
    • Korean Journal of Dental Materials
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    • v.44 no.3
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    • pp.273-280
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    • 2017
  • The aim of the present study was to investigate the effects of specimen dimension on the flexural properties and testing reliability of dental composite resin. The composite resin was prepared experimentally by mixing a resin matrix with silanated micrometer glass filler at 50 vol%. Flexural specimens with various dimension in specimen's width were fabricated by light curing using a split metal mold; $25{\times}2{\times}2mm$, $25{\times}2{\times}4mm$, $25{\times}2{\times}6mm$ in length ${\times}$ height ${\times}$ width. The flexural strength and modulus were determined according to ISO 4049 test protocol at a span length of 20 mm (normal-flexural strength; NFS). Another flexural test was conducted using mini-sized specimens ($12{\times}2{\times}2mm$, $12{\times}2{\times}4mm$, $12{\times}2{\times}6mm$) from the broken specimens at a span length of 10 mm (mini-flexural strength; MFS). Data were analyzed with ANOVA and Duncan's post-hoc test and the test reliability was evaluated by Weibull analysis. Results showed that there are generally no significant difference in flexural strength with the increase in the specimen width in NFS and MFS tests. However, the test reliability of flexural strength based on Weibull analysis was largely changed with the variables in the dimension of width and span length. The flexural modulus of NFS was increased as the dimension of specimens width increased while there was no trend in flexural modulus of MFS test. Overall results recommend that the evaluation of flexural properties and the reliability of dental composite resins should be performed with more than one test method.

Microbial Qualities of Parasites and Foodborne Pathogens in Ready to Eat (RTE) Fresh-cut Produces at the On/Offline Markets (즉석섭취 신선편의 절단 과일 및 채소의 원충류 및 병원성 식중독균의 미생물학적 품질 실태 연구)

  • Jeon, Ji Hye;Roh, Jun Hye;Lee, Chae Lim;Kim, Geun Hyang;Lee, Jeong Yeon;Yoon, Ki Sun
    • Journal of Food Hygiene and Safety
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    • v.37 no.2
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    • pp.87-96
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    • 2022
  • Recently, the purchase of fresh-cut produce and meal kits has increased. Ready-to-eat (RTE) fresh-cut products have potentially hazard of cross-contamination of various microorganisms in the processes of peeling, slicing, dicing, and shredding. There are frequent cases of protozoa food poisoning, such as Cyclospora and Cryptosporidium, caused by fresh-cut products. The objective of the study is to investigate the microbiological qualities of various types of RTE fresh-cut products in the domestic on/offline markets. RTE fresh-cut fruits cup (n=100), fresh-cut vegetables (n=50), and vegetables in meal kits (Vietnamese spring rolls and white radish rolls kits, n=50) were seasonally analyzed. The contamination levels of hygienic indicator organisms, yeast and mold (YM), and foodborne pathogens (Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella spp., and Escherichia coli O157:H7) were monitored. Overall, the lowest microbiological qualities of meal kits vegetables were observed, followed by RTE fresh-cut fruits cup and fresh-cut vegetables. Contamination levels of total aerobic bacteria, coliforms, and YM in meal kits vegetables were 5.91, 3.90, and 4.71 logs CFU/g, respectively. From the qualitative analysis, 6 out of 200 RTE fresh-cut products (3%) returned positive result for S. aureus. From the quantitative analysis, the contamination levels of S. aureus in purple cabbage from a meal-kit and fresh-cut pineapple were below the acceptable limit (100 CFU/g). Staphylococcus enterotoxin seg and sei genes were detected in RTE fresh-cut celery and red cabbage from meal-kits, respectively. S. aureus contamination must be carefully controlled during the manufacturing processes of RTE fresh-cut products. Neither Cyclospora cayetanensis nor Cryptosporidium parvum was detected in the samples of RTE fresh-cut products and vegetables from meal-kits from the Korean retail markets.

Evaluation of a Compact Dry Method for Enumerating Bacteria in Contaminated Foods (식품 오염 미생물 분석을 위한 컴팩트 드라이법 평가)

  • Soo-Jin Jung;Sangha Han;June Gu Kang;Min Su Song;Hyewon Song;Harim Lee;Jisu Yu;Kyung Ok Lee;Sang-Do Ha
    • Journal of Food Hygiene and Safety
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    • v.39 no.4
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    • pp.304-311
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    • 2024
  • The present study aimed to compare the accuracy of the compact dry and culture plate methods for natural flora (yeasts/molds, coliforms, and total bacterial count) and artificial inoculum (Escherichia coli) in dosirak (meat-based), meal kits (seafood-based), and Doenjang (traditional food). Compact dry TC, EC, CF, and YMR were compared with culture plate methods using a suitable medium for each bacterium. The total bacterial count, coliforms, yeasts/molds, and E. coli were assessed with 3M Petrifilm (aerobic bacterial, coliform, yeast/mold, and E. coli count plates) using culture plate methods. Analysis of the recovery rates of target microorganisms in the three food samples showed that the compact dry method for total bacteria, coliforms, and E. coli exhibited recovery capabilities equivalent to those of the culture plate and Petrifilm methods, with no significant differences (P>0.05). Overall, compact dry TC, CF, and EC showed a good correlation between the methods used in this study, indicating rapid and convenient microbial enumeration by saving time and requiring less space.