• Journal of Conservation Science
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• v.36 no.2
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• pp.123-142
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• 2020

We attempted an experimental study of lost-wax casting to reconstruct the Gilt-bronze pensive Bodhisattva; The main object we aimed to reconstruct was the Korean national treasure No.83, Gilt-bronze pensive Bodhisattva(Maitreya); thus, we scientifically analyzed and measured the alloy ratio and casting method. Initially, the alloy ratio we used during the preliminary experiment was Cu:Sn:Pb = 95.5:4.0:0.5; we then increased tin and lead by 2.5% to consider vaporization during the main experiment. After applying lost-wax casting, we realized that 30% rosin contributed to proper hardness after the wax hardness experiment. The microstructure revealed normal casting character(α-Cu, δ), and the results of the chemical analysis are identical to those of previous studies. The analysis of the mold suggests the presence of quartz powder for structural stability and fire-resistance along with other organic materials whose contribution is still unknown. We expect that our research will serve to provide basic data for advanced studies in the future.

• Journal of Technologic Dentistry
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• v.34 no.2
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• pp.113-119
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• 2012

Purpose: Zirconia blocks for all ceramic dentures are divided into two groups. One is pre-heated block and the other is binder added block. In this study, the possibility of recycling the remained parts of binder added block after CAD/CAM machining with slip casting process was investigated. Methods: Owing to the binder added block contain large amount of organic matter, Binder burn-out was must be carried out before ball milling for preparing the casting slip. Binder burn-out was accomplished at $600^{\circ}C$ for 10 hours. Ball milling was performed with 5mm zirconia ball and 60mm polyethylene bottle. From 0% to 5% at 1% intervals of alumina was added to zirconia powder for preparing slip. Solid casting was achieved with plaster mold. Cast bodies were dried and sintered at $1,500^{\circ}C$ for 1 hour. Linear shrinkage, apparent porosity, water absorption, bulk density, and flexural strength were tested. Microstructures were observed by SEM, EDS and XRD analysis were executed. Results: Optimum slips for casting was prepared with 300g ball, 100g powder, and 180g distilled water. Cast body without alumina showed 26% of linear shrinkage, 6.07 of apparent density, and 470MPa of three point bend strength. On the other hand, as received zirconia block, which was sintered at the same conditions, showed 23% of linear shrinkage, 6.10 of apparent density, and 680MPa of three point bend strength. When 3% of alumina was added to zirconia, sintered body showed 23% of linear shrinkage, 6.10 of apparent density, and 780MPa of three point bend strength. SEM photomicrographs and EDS analysis showed alumina particles uniformly dispersed in zirconia matrix, and XRD analysis showed no phase transformation of tetragonal zirconia particles was occurred when alumina was added. Conclusion: According to the all of this experimental results, 3% of alumina added cast zirconia body showed excellent mechanical properties more than as received binder containing zirconia block.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.3
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• pp.229-236
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• 2020

Sixty-five molds isolated from clinical specimens were included in this study. All the isolates were molds that could be identified morphologically, strains that are difficult to identify because of morphological similarities, and strains that require species-level identification. PCR and direct sequencing were performed to target the internal transcribed spacer (ITS) region, the D1/D2 region, and the β-tubulin gene. Comparative sequence analysis using the GenBank database was performed using the basic local alignment search tool (BLAST) algorithm. The fungi identified morphologically to the genus level were 67%. Sequencing analysis was performed on 62 genera and species level of the 65 strains. Discrepancies were 14 (21.5%) of the 65 strains between the results of phenotypic and molecular identification. B. dermatitidis, T. marneffei, and G. argillacea were identified for the first time in Korea using the DNA sequencing method. Morphological identification is a very useful method in terms of the reporting time and costs in cases of frequently isolated and rapid growth, such as Aspergillus. When molecular methods are employed, the cost and clinical significance should be considered. On the other hand, the molecular identification of molds can provide fast and accurate results.

• Conservation Science in Museum
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• v.4
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• pp.23-32
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• 2003

As the kettle excavated from a site in Hwangnam-dong, Gyeongju was presumed to have been used as a melting crucible in a glass production workplace, we decided to prove its usage by scientific analysis. First, we performed conservation treatment to find the original status of the kettle, and then SEM-EDS and XRD analysis of the five corrosions created on the surface of the kettle. The fragment of the spout, which played a crucial role for the kettle to be considered as a melting crucible, was discovered during the conservation treatment. So the mouth rim of the kettle was restored to perfection. When we observed the microstructure of the metal sample, it was proved to be cast iron gradually cooled without heat treatment. In the corrosion products, the main components were Fe and O and other components such as P, Si, Ca, and S were found. The main compounds were quartz, vivianite, goetheite, akaganite, lepidocrocite, hematite, etc. Although these components were used as raw materials for making glass, these were found not in the melting status but mere raw materials. This can be an evidence to show these site where the kettle was excavated had been a glass production workplace. However, it is not sure that the kettle was used as a melting crucible. Moreover, if we consider the organic mold and sand clay accumulated in the well site when the kettle was excavated at first, we can see this as a formative factor of the corrosions of the kettle. Therefore, we concluded that the kettle is a typical cast iron and was not used as a melting crucible of glass.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.11
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• pp.4924-4931
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• 2011

This research performed to evaluate a production processes reporting by the HACCP system of green vegetable juice products, containing lactic acid bacteria, stage of processing raw materials agricultural products and production facilities of general bacteria and pathogenic micro organism. General bacteria are found from four samples of storage of agricultural products at process stage and water was detected 8.67~14.67 CFU/ml. However, all samples were detected less than 105 CFU/ml as a legal standards after the process of UV sterilization. For the outcome of experiment of E.coli, E.coli O157:H7, B.cereus, L.moonocytogenes, Salmonella spp, Staph.aureus as the food poisoning bacterial, E.coli was detected until UV pre-step process in storage process and B.cereus was detected partly till 1st washing. Since all bacterial, Yeast and Mold are detected in main materials, pre-control method is a necessary to establish for decreasing with a number of initial bacteria of main materials and it is considered to establish the effective ways of washing and sterilization such as production facilities for cross contamination prevention of bacteria and Sthaphylococcus. Based on above results, the process of UV sterilization should be managed with CCP as an important process to reduce or eliminate the general and food poisoning bacterial of green vegetable juice products, including lactic acid bacteria. Therefore, it is considered to need an exhaustive HACCP plan such as control manual of UV sterilization, solution method, verification, education and training and record management.

• Journal of Environmental Health Sciences
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• v.47 no.5
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• pp.432-445
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• 2021

Background: The European Centre for Ecotoxicology and Toxicology of Chemicals' Targeted Risk Assessment (ECETOC TRA) tool has been recognized by EU REACH as a preferred approach for calculating worker health risks from chemicals. Objectives: The applicability of the ECETOC TRA to occupational exposure estimation from industrial uses of methanol was studied by inputting surveyed and varied parameters for TRA estimation as well as through comparison with measured data. Methods: Information on uses of methanol was collected from seven working environment monitoring reports along with the measured exposure data. Input parameters for TRA estimation such as operating conditions (OCs), risk management measures (RMMs) and process categories (PROCs) were surveyed. To compare with measured exposures, parameters from the surveyed conditions of ventilation but no use of respiratory protection were applied. Results: PROCs 4, 5, 8a, 10, and 15 were assigned to ten uses of methanol. The uses include as a solvent for manufacturing sun cream, surfactants, dyestuffs, films and adhesives. Methanol was also used as a component in a release agent, hardening media and mold wash for cast products as well as a component of hard-coating solution and a viscosity-controlling agent for manufacturing glass lenses. PROC 8a and PROC 10 of a cast product manufacturer without LEV (local exhaust ventilation) and general ventilation as well as no respiratory protection resulted in the highest exposure to methanol. Assuming the identical worst OCs and RMMs for all uses, exposures from PROC 5, 8a, and 10 were the same and the highest followed by PROC 4 and 15. The estimation resulted in higher exposures in nine uses except one use where measured exposure approximated exposures without RMMs. Conclusions: The role of ECETOC TRA as a conservative exposure assessment tool was confirmed by comparison with measured data. Moreover, it can guide which RMMs should be applied for the safe use of methanol.

• Journal of Korea Foundry Society
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• v.43 no.6
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• pp.302-309
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• 2023

In general, castings often have complex shapes and significant variations in thickness within a single product, making grid generation for simulations challenging. Casting flows involve multiphase flows, requiring the tracking of the boundary between air and molten metal. Additionally, considerable time is spent calculating pressure fields due to density differences in a numerical analysis. For these reasons, the Cartesian grid system has traditionally been used in mold filling simulations. However, orthogonal grids fail to represent shapes accurately, leading to a momentum loss caused by the stair-like grid patterns on curved and sloped surfaces. This can alter the flow of molten metals and result in incorrect casting process designs. To address this issue, simulations in the Cartesian grid system involve creating a large number of grids to represent shapes more accurately. Alternatively, the Cut Cell method can be applied to address the problems arising from the Cartesian grid system. In this study, analysis results based on the number of grid in the Cartesian grid system for a casting flow analysis were compared with results obtained using the Cut Cell method. Casting flow simulations of actual products during various casting processes were also conducted, and these results were analyzed with and without applying the Cut Cell method.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.11
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• pp.4956-4962
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• 2012

Among the various parts of automobile, automotive seat is the most fundamental item that ride comfort can be evaluated as the direct contact part with human body. Automotive seat must have the sufficient rigidity and strength at the same time with ride comfort. In this study, cushion frame and back frame at car seat are modelled with 3D. There are structural simulation analyses about 3 kinds of tests on torsion strength, vertical load strength and back frame strength. In the analysis result, the initial total deformation and the permanent total deformation has the maximum values of 5.4821 mm and 0.02539mm respectively at the torsion strength test of cushion frame. Total deformations at front and rear end parts of cushion frame become the values of 2.1159mm and 0.0606mm respectively at the test of vertical load strength of cushion frame. In case of more than this load, the maximum value of total deformation also becomes 3.1739mm. The maximum value of total deformation becomes 0.18634mm at 3 kinds of the strength tests on back frame. By the study result of no excessive deformation and no fracture cushion frame and back frame at automotive seat, the sufficient rigidity and strength to guarantee the safety of passenger can be verified.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.5
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• pp.649-654
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• 2006

It was investigated the effects of pre-treatment method on the microbes on the surface of radish (Raphanus sativus L.) leaves. Independent variables put in water washing ($X_1$), microwave treatment ($X_2$) and steam treatment ($X_3$) using central composite design and response surface analysis. It was not detected in the pathogenic microbes, Samonella spp., Camphylobacter spp., Vibrio spp., Shigella spp., Staphyloccocus spp., on the surface of collected radish leaves without pre-treatment. But general microbes showed $3.90{\times}10^5{\sim}1.20{\times}10^7CFU/g$ of total microbes, $1.10{\times}10^2{\sim}2.00{\times}10^5CFU/g$ of E. coli, $2.40{\times}10^3{\sim}3.55{\times}10^6CFU/g$ of yeast/mold on the surface of various radish leaves and lactic acid bacteria was detected or not according to collected samples. The best method of pre-treatment was steam treatment on the microbe reduction effect of samples surface. Also, the multiplex regression coefficients analysis was calculated three independent variables ($X_1,\;X_2,\;X_3$) and dependent variables (total microbes, lactic acid bacteria and yeast/molds). It showed high correlation $R^2$, 0.89, 0.87, 0.85, respectively. For effective reduction of surface microbes, the best method was water washing with microwave or steam treatment at the same time.

• Korean journal of food and cookery science
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• v.25 no.2
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• pp.252-259
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• 2009

Ergosterol is the significant component of the cell wall of fungi. Its presence is regarded as evidence of fungi contamination in grain and other foods. Many studies on ergosterol detection have been carried out using chemical methods, but those methods required complicated pre-treatments and long analysis times. In this study, an amperometric biosensor was developed for fast and precise ergosterol detection. The biosensor system used the electron transfer of hydrogen peroxide produced from the reaction of ergosterol with cholesterol oxidase. The biosensor system consisted of a peristaltic pump, a syringe loading sample injector, an enzyme reactor, a fabricated flow-through cell containing a working electrode, a reference electrode and a counter electrode, and a potentiostat/recorder. The working electrode was prepared by coating modified multi-wall carbon nanotube (MWNT) on glassy carbon electrode. The $MWNT-NH_2$ coated glassy carbon electrode linearly responded to hydrogen peroxide in the range of $1{\times}10^{-5}{\sim}8{\times}10^{-5}$ M with a detection limit of $10^{-7}$ M in the basic performance test. The currents produced from the ergosterol biosensor showed the linearity in a range from $1.0{\times}10^{-6}$ M to $1.0{\times}10^{-5}$ M ergosterol.