• Title/Summary/Keyword: Module cell

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Cloning and Characterization of Xylanase 11B Gene from Paenibacillus woosongensis (Paenibacillus woosongensis의 Xylanase 11B 유전자 클로닝과 특성분석)

  • Yoon, Ki-Hong
    • Microbiology and Biotechnology Letters
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    • v.45 no.2
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    • pp.155-161
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    • 2017
  • A gene coding for the xylanase predicted from the partial genomic sequence of Paenibacillus woosongensis was cloned by PCR amplification and sequenced completely. This xylanase gene, designated xyn11B, consisted of 1,071 nucleotides encoding a polypeptide of 356 amino acid residues. Based on the deduced amino acid sequence, Xyn11B was identified to be a modular enzyme, including a single carbohydrate-binding module besides the catalytic domain, and was highly homologous to xylanases belonging to glycosyl hydrolase family 11. The SignalP4.1 server predicted a stretch of 26 residues in the N-terminus to be the signal peptide. Using DEAE-Sepharose and Phenyl-Sepharose column chromatography, Xyn11B was partially purified from the cell-free extract of recombinant Escherichia coli carrying a copy of the P. woosongensis xyn11B gene. The partially purified Xyn11B protein showed maximal activity at $50^{\circ}C$ and pH 6.5. The enzyme was more active on arabinoxylan than on oat spelt xylan and birchwood xylan, whereas it did not exhibit activity towards carboxymethylcellulose, mannan, and para-nitrophenyl-${\beta}$-xylopyranoside. The activity of Xyn11B was slightly increased by $Ca^{2+}$ and $Mg^{2+}$, but was significantly inhibited by $Cu^{2+}$, $Ni^{2+}$, $Fe^{3+}$, and $Mn^{2+}$, and completely inhibited by SDS.

The Salt Removal Efficiency Characteristics of Carbon Electrodes Using Fabric Current Collector with High Tensile Strength in a Capacitive Deionization Process (인장강도가 뛰어난 직물집전체를 이용한 탄소전극의 축전식 탈염공정에서의 제염효과)

  • Seong, Du-Ri;Kim, Dae Su
    • Korean Chemical Engineering Research
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    • v.58 no.3
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    • pp.466-473
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    • 2020
  • Fabric current collector can be a promising electrode material for Capacitive Deionization (CDI) system that can achieve energy-efficient desalination of water. The one of the most attractive feature of the fabric current collector is its high tensile strength, which can be an alternative to the low mechanical strength of the graphite foil electrode. Another advantage is that the textile properties can easily make shapes by simple cutting, and the porosity and inter-fiber space which can assist facile flow of the aqueous medium. The fibers used in this study were made of woven structures using a spinning yarn using conductive LM fiber and carbon fiber, with tensile strength of 319 MPa, about 60 times stronger than graphite foil. The results were analyzed by measuring the salt removal efficiency by changing the viscosity of electrode slurry, adsorption voltage, flow rate of the aqueous medium, and concentration of the aqueous medium. Under the conditions of NaCl 200 mg/L, 20ml/min and adsorption voltage 1.5 V, salt removal efficiency of 43.9% in unit cells and 59.8% in modules stacked with 100 cells were shown, respectively. In unit cells, salt removal efficiency increases as the adsorption voltage increase to 1.3, 1.4 and 1.5 V. However, increasing to 1.6 and 1.7 V reduced salt removal efficiency. However, the 100-cell-stacked module showed a moderate increase in salt removal efficiency even at voltages above 1.5 V. The salt removal rate decreased when the flow rate of the feed was increased, and the salt removal rate decreased when the concentration of the feed was increased. This work shows that fabric current collector can be an alternative of a graphite foil.

Development of a Mountainous Area Monitoring System based on IoT Technology (IoT 기술 기반의 산악지 모니터링 시스템 개발)

  • Kim, Kyoon-Tai
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.3
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    • pp.437-446
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    • 2017
  • 70 percent of Korea's territory is covered with mountains, whose difficult conditions can cause damage to facilities. Recently, the demand for facilities related to outdoor activities including monorails has been on the rise, and such facilities are much more likely to become damaged. For this reason, a monitoring system applying IoT to mountainous areas was developed and its applicability is evaluated in this study. The current status of the existing mountainous facilities and monitoring systems were reviewed, and the current wired monitoring technology was analyzed. A scenario for IoT-based monitoring was developed, and then sensor nodes were developed, which include an RF-communication module and interface, power-supply and solar-cell. A testbed was set up at K University. The same data was collected by the wireless system as had been collected by the wired one. The study findings are as follows. Firstly, by using the wireless system, it is estimated that the construction duration can be reduced by about 25 percent, while the construction costs can be reduced by about 3~52 percent. Secondly, the safety of the construction workers can be improved by making the working conditions less dangerous, such as by eliminating the need to transport cables.

Study on crystallization behavior of an ethylene-polypropylene copolymer based encapsulant for photovoltaic application (태양전지 봉지재용 에틸렌-프로펠렌 공중합체의 결정화 거동에 관한 연구)

  • Son, Younggon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.12
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    • pp.737-742
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    • 2016
  • We prepared five different ethylene-propylene copolymers (EPCs) for use as the encapsulant of a photovoltaic module. All of the polymers were of commercial grade from ExxonMobile company and had different ethylene/propylene compositions. The crystallization behaviors and crystal structures of the polymers were analyzed by differential scanning calorimetry and wide angle X-ray scattering diffractometry, respectively. We observed the general trend that the degree of crystallization, density and glass transition temperature of the EPCs decreased with increasing ethylene content. However, an unexpected result was also observed: the EPC with the highest ethylene content (22.2 mol. %) showed the highest melting temperature. As a result, the EPC with 22.2 mol. % of ethylene shows the highest light transmittance, due to its having the lowest degree of crystallization and highest thermal creep resistance. This abnormal result is attributed to the blocky structure prepared by ExxonMobile's special catalyst technology. It was also observed that new additional melting peaks appeared as the crystallization time increased. Using wide angle X-ray scattering diffractometry, it was confirmed that these additional peaks originated from the formation of a new crystal structure caused by annealing.

$H_{2}S$ Removal and $CO_{2}/CH_{4}$ Separation of Ternary Mixtures Using Polyimide Hollow Fiber Membrane (폴리이미드 중공사막을 이용한 혼합기체로부터 $H_{2}S$ 제거 및 $CO_{2}/CH_{4}$ 분리에 관한 연구)

  • Park, Bo-Ryoung;Kim, Dae-Hoon;Jo, Hang-Dae;Seo, Yong-Seog;Hwang, Taek-Sung;Lee, Hyung-Keun
    • Korean Chemical Engineering Research
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    • v.49 no.2
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    • pp.250-255
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    • 2011
  • In this study, by using the polymeric membrane separation process, the $CO_{2}/CH_{4}$ separation and $H_{2}S$ removal from biogas were performed in order to $CH_{4}$ purification and enrichment for the fuel cell energy source application. Fibers were spun by dry/wet phase inversion method. The module was manufactured by fabricating fibers after surface coating with silicone elastomer. The scanning electron microscopy(SEM) studies showed that the produced fibers typically had an asymmetric structure; a dense top layer supported by a porous, sponge substructure. The permeance of $CO_{2}$ and $CO_{2}/CH_{4}$ selectivity increased with pressure and temperature. Mixture gas with increasing pressure and temperature, removal efficiency of the $CO_{2}$ and $H_{2}S$ were decreased while concentration of $CH_{4}$ was increased up to 100%. When retentate flow rate was increased with the decreasing of pressure and temperature the $CH_{4}$ recovery ratio in retentate side was increased while the $CH_{4}$ purity in retentate side was decreased.

Area-efficient Interpolation Architecture for Soft-Decision List Decoding of Reed-Solomon Codes (연판정 Reed-Solomon 리스트 디코딩을 위한 저복잡도 Interpolation 구조)

  • Lee, Sungman;Park, Taegeun
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.3
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    • pp.59-67
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    • 2013
  • Reed-Solomon (RS) codes are powerful error-correcting codes used in diverse applications. Recently, algebraic soft-decision decoding algorithm for RS codes that can correct the errors beyond the error correcting bound has been proposed. The algorithm requires very intensive computations for interpolation, therefore an efficient VLSI architecture, which is realizable in hardware with a moderate hardware complexity, is mandatory for various applications. In this paper, we propose an efficient architecture with low hardware complexity for interpolation in soft-decision list decoding of Reed-Solomon codes. The proposed architecture processes the candidate polynomial in such a way that the terms of X degrees are processed in serial and the terms of Y degrees are processed in parallel. The processing order of candidate polynomials adaptively changes to increase the efficiency of memory access for coefficients; this minimizes the internal registers and the number of memory accesses and simplifies the memory structure by combining and storing data in memory. Also, the proposed architecture shows high hardware efficiency, since each module is balanced in terms of latency and the modules are maximally overlapped in schedule. The proposed interpolation architecture for the (255, 239) RS list decoder is designed and synthesized using the DongbuHitek $0.18{\mu}m$ standard cell library, the number of gate counts is 25.1K and the maximum operating frequency is 200 MHz.

FPGA Implementation of a Grant Distribution Algorithm for the MAC in the ATM-PON (ATM-PON에서 MAC을 위한 승인분배 알고리즘의 FPGA 구현)

  • Kim, Tae-Min;Chung, Hae;Shin, Gun-Soon;Kim, Jin-Hee
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.38 no.10
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    • pp.1-9
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    • 2001
  • The MAC (Medium Access Control) protocol is needed for the OLT(Optical Line Termination) to allocate bandwidth to ONUs(Optical Network Units) and ONTs(Optical Network Terminations) dynamically in the ATM PON(Passive Optical Network). With the protocol, the OLT gathers ONUs' informations and provides grants efficiently to each ONU. Two important functions of the MAC protocol is the grant request procedure and the grant distribution algrithm. The latter has the greatest arithmetic portion in the TC(Transmission Convergence) module, occupies a relatively large portion of the overall chip area, has often been the limiting factor in terms of speed, and should be designed to guarantee the quality of service for various traffics. In this paper, we implement the MAC with the FPGA which can allocate grants dynamically according to the queue length information and the number of active ONUs and distribute grants uniformly to minimize the cell delay variation for each ONU. The structure of the MAC scheduler for the dynamic bandwidth assignment has a programmable look-up table. Also, it has a simple structure, the less chip area, and the lower delay time.

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A Study on Low Power Energy Transfer Circuits of the Non Contact Method by means of Solar Generation (태양광 발전에 의한 비접촉 방식 저 전력 에너지 전송회로에 관한 연구)

  • Hwang, Lark-Hoon;Na, Seung-Kwon;Kim, Jong-Rae;Choi, Gi-Ho;Kim, Jin-Seon
    • Journal of Advanced Navigation Technology
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    • v.18 no.1
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    • pp.35-43
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    • 2014
  • In this paper, it is about to non-contact wireless power transmission according to various conditions of self induction principle between the two planar coils at a transmission unit and a receiving unit based on the theory of wireless power transmission. The experiments are occurred in order to power transfer of noncontact method from designed wireless circuits in the primely coil and secondary coil, and the applying to Half Bridge Resonant converter transmission unit and receiving unit. and that were able to prepared circumstance to calculate of the output voltage and power source. The main power of the inductive coupling the resonant converter at the transmission unit is converted electrical energy using the solar cell module and artificial light source (halogen lamp) as a replace light and received 24 V power supply from solar power was used a input power source for the wireless power transmission device. Experimental results, to received of power is used to illuminate the lighting and to charge the battery in receiving circuit.And the wireless power transmission efficiency measured at the output side of the transmission unit is obtained about 70% to 89% compared to input power of receiving unit.In addition, efficiency were tested through ID verification method and comparing the phase difference between the voltage when foreign substances interfere with wireless power transmission.

Effects of Low-Level Laser Irradiation on the ALP Activity and Calcified Module Formation of Rat Osteoblastic Cell (저수준레이저(GaAs 반도체)조사가 골모세포의 알칼리성 인산분해효소의 활성과 석회화결절의 형성에 미치는 영향)

  • Kyung-Hun Lee;Ki-Suk Kim
    • Journal of Oral Medicine and Pain
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    • v.21 no.2
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    • pp.279-291
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    • 1996
  • 저수준레에저요법에 대해서는 지난 10여년간 의학계 및 치과계에서 임상적으로 사용하여 좋은 결과가 있다고 많은 보고가 발표되고 있다. 특히 치근의 골결손에 관한 연구에서는 전기요법, 초음파요법, 전자장요법 등 뿐만아니라 저수준레이저를 사용하여 골절부내 Callus형성이 촉진되었음을 보고하고 새로운 치료법의 하나가 될 수 있음을 제안한 바도 있다. 본 연구에서는 칼륨비소를 다이오드로 사용한 저수준레이저조사가 골결손의 치유에 어떠한 영향을 미치는지 확인하고자 골모세포의 알칼리성 인산분해효소의 활성화와 석회화결절의 형성을 평가함으로 골모세포의 기능을 조사코저하였다. 실험은 첫째, 9개군으로 나누어 레이저 조사기간에 따른 알칼리성 인산분해효소의 활성화를 조사하였고, 둘째, 이를 근거로 9일간 계속 매일 1회 1.3 J/cm2의 레이저를 조사한 후 펄스의 종류별 차이를 비교하였으며, 세째,레이저펄스별 석회차 결절의 형성 정도를 광학현미경으로 관찰하여 비교분석하였다. 결과, 7일 계속 레이저를 조사한 경우 다른 군에 비해 서서히 ALP의 활성이 증가하였으나 유의한 차이는 없었으며. 따라서 9일동안 레이저를 계속 조사한 경우에는 전체 에너지량이 5.895 J/cm2 인 펄스13과 15가 뚜렷하게 유의한 증가를 보여주었다. 그러나 석회화결절의 형성은 전체 에너지량이 2.546 J/cm2 인 펄스11에서 가장 많았다. 결론적으로 골형성이나 알칼리성 인산분해효소의 활성을 촉진하는 데에는 적절한 레이저 조사조건이 필요하나, 알칼리성 인산분해효소의 활성을 촉진한 펄스와 석회화결절의 형성을 촉진하는 펄스가 서로 다르게 나타난 것은 골형성을 촉진하는 여러요인 들이 저수준레이저에 자극받았을 가능성이 높음을 보여준다 이러한 결과들로 보아 저수준레이저는 골모세포의 기능을 자극하여 골결손의 치유를 개선하는 데 도움될 것이라 사료된다.다. 각 백서의 양측 창상중 하나는 1,3,5,7일 마다 각 실험의 방법에 따라 레이저를 조사하고 실험동물의 다른 창상은 대조군으로서 사용하였다. 모든 창상의 면적은 실험 1,3,5,7 일째에 일정한 거리에서 사진촬영하여 면적계를 이용, 측정한 후 통계적인 의의를 조사하였다. 본 연구의 결과는 저수준레이저는 특정 조건하에서 S. aureus의 증식을 촉진하였다. 그러나 S. aureus에 감염된 창상을 저수준레이저로 조사시 치유가 촉진되었다. 중앙 조사법고 주변조사법에 의한 창상치유효과는 통계적인 의의가 보이지 않았다. 따라서 결론적으로 S. aureus 에 감염된 창상에 직접 또는 간접적이든 pulse의 종류에 관계없이 조사하는 경우 치유효과가 나타나는 것은 정사주위 조직의 LLLI 자극효과가 염증의 확산을 억제한다고 말할수 있다.4/1$0^{\circ}C$에서는 Shoa-Nan-Tsan과 Lenkwang이 가장 높았으며 백앙벼는 3 온도 조건 모두에서 활성이 낮았다. 발아소요일수와 amylase 활성과는 유의적인 정의 상관관계를 보였다., 다다조, 미국의 건답직파재배 품종 등이었으며 우리 나라 육성종들은 모두 지중에서 신장이 멈추어 제1본엽이 지중에서 추출하였으며, Scm파종심에서 불완전엽이 지면을 뚫고 나오는 품종은 Chinsura Boro뿐이었고 Nato, Labelle, Weld Pally, Italliconaverneco 등도 지면 가까이 까지 신장하였다. 6. 50% 출아일수는 제2절간장을 제외 한 모든 유아 형질의 신장도와 유의한 부의 상관을 보였는데 가장 높은 상관을 보인 것은 중배축장+제1절간장+불완전옆장이었으며, 다음이 불완전엽장이 었다. 7. 출아율은 중배축장+제1절간장+불완전엽장, 중배축장+초엽 장과 모든 파종심에서 높은 정의 상관을 보여 제1본엽의 추출 위치가 높을수록

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Electrical and Fluidic Characterization of Microelectrofluidic Bench Fabricated Using UV-curable Polymer (UV경화성 폴리머를 이용한 미소유체 통합접속 벤치 개발 및 전기/유체적 특성평가)

  • Youn, Se-Chan;Jin, Young-Hyun;Cho, Young-Ho
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.36 no.5
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    • pp.475-479
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    • 2012
  • We present a novel polymer fabrication process involving direct UV patterning of a hyperbranched polymer, AEO3000. Compared to PDMS, which is the most widely used polymer in bioMEMS devices, the present polymer has advantages with regard to electrode integration and fast fabrication. We designed a four-chip microelectrofluidic bench having three electrical pads and two fluidic I/O ports. We integrated a microfluidic mixer and a cell separator on the bench to characterize the interconnection performance and sample manipulation. Electrical and fluidic characterization of the microfluidic bench was performed. The measured electrical contact resistance was $0.75{\pm}0.44{\Omega}$, which is small enough for electrical applications, and the pressure drop was 8.3 kPa, which was 39.3% of the value in the tubing method. By performing yeast mixing and a separation test in the integrated module on the bench, we successfully showed that the interconnected chips could be used for bio-sample manipulation.