• Macromolecular Research
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• v.17 no.11
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• pp.850-855
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• 2009

Keratin is an important protein used in wound healing and tissue recovery. In this study, keratin was modified chemically with iodoacetic acid (IAA) to enhance its solubility in organic solvent. Poly(hydroxybutylate-co-hydroxyvalerate) (PHBV) and modified keratin were dissolved in hexafluoroisopropanol (HFIP) and electrospun to produce nanofibrous mats. The resulting mats were surface-characterized by ATR-FTIR, field-emission scanning electron microscopy (FE-SEM) and electron spectroscopy for chemical analysis (ESCA). The pure m-keratin mat was cross-linked with glutaraldehyde vapor to make it insoluble in water. The biodegradation test in vitro showed that the mats could be biodegraded by PHB depolymerase and trypsin aqueous solution. The results of the cell adhesion experiment showed that the NIH 3T3 cells adhered more to the PHBV/m-keratin nanofibrous mats than the PHBV film. The BrdU assay showed that the keratin and PHBV/m-keratin nanofibrous mats could accelerate the proliferation of fibroblast cells compared to the PHBV nanofibrous mats.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.2D
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• pp.243-248
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• 2008

Moisture damage of asphalt pavements can usually occur because of the loss of adhesion and cohesion between the asphalt binder and aggregate in the asphalt mixture due to presence of water. And this is one of the causes that is effect on the main distress of asphalt pavement. The objective of this study is to find out moisture damage characteristics of asphalt pavement. Effects of this study changes of the material properties and resistance characteristics of moisture damage on the asphalt mixtures under various temperatures and repeated immersion using indirect tensile test and modify Lottman test were evaluated during this study. The asphalt mixtures were produced using straight asphalt binder, SBS modified asphalt binder and aggregates. The material properties (resilient modulus, indirect tensile strength, failure energy and $DCSE_f$) of the asphalt mixtures were generally decreased with increasing to moisture damage caused by the number of repeated immersion. The decrease ratios of material properties by repeated immersion on SBS modified asphalt mixtures were lower than those of straight asphalt mixtures at all three test temperatures. As a conclusion, current criterion for evaluation moisture damage of asphalt mixtures is difficult for using distinction standard because of the limited evaluation criterion with one time immersion and single material property. Based on this research, to evaluate long term moisture damage on asphalt mixtures, material property tests of various kinds with repeated immersion test are considered.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2001.10a
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• pp.121-124
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• 2001

Surface-modified silica holds considerable promise in the development of advanced materials for good mechanical properties and stability. In this work, the surface and mechanical interfacial properties of silicas treated with silane coupling agents, such as Y-methacryloxy propyl trimethoxy silane (MPS). Y-glycidoxy propyl trimethoxy silane (GPS), and Y-mercapto propyl trimethoxy silane (MCPS), are investigated. The effect of silane surface treatments of silica on the surface properties and surface energetics are studied in terms of surface functional values and contact angle measurements. And their mechanical interfacial properties of the silica/rubber composites are studied by the composite tearing energy ($G_{IIIC}$). As a result. the mechanical interfacial properties are improved in the case of silane-treated composites compared with untreated one. It reveals that the functional groups on silica surface by silane surface treatments play an important role in improving the degree of adhesion at interfaces in a silica-filled rubber system.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2001.10a
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• pp.125-128
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• 2001

In this wort, the $Na^+-MMT$ has organically modified with silane intercalant to prepare the polymer/clay nanocomposites. The pH. X-ray diffraction (XRD), and contact angles were used to analyze the surface properties of clay and the exfoliation phenomenon of clay interlayer, The mechanical interfacial properties of epoxy/clay nanocomposites were investigated by three-point bending test. From the experimental results. the surface modification made by silane intercalant on clay surface leads to an increase of distance of silicate layers, surface acid value. and electron acceptor parameter of organoclay. The treatments are also necessary and useful for epoxy to intercalate into the interlayer by interacting of electron donor-accepter between basic epoxy and clay surface. The mechanical interfacial properties of the nanocomposites was improved by the presence of dispersed clay nanolayer containing low content of organoclay in comparison with the conventional, which increase the interfacial adhesion between dispersed clay and epoxy resins.

• Korea-Australia Rheology Journal
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• v.12 no.2
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• pp.135-141
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• 2000

The linear viscoelastic behavior of acrylonitrile-butadiene-styrene (ABS) polymers with different rubber content has been investigated in the frame of a linear viscoelastic model, which takes into account the inter-connectivity of the dispersed rubber particles. The model developed in our previous work has been shown to properly predict the low frequency plateau for the storage modulus, which is generally observed in polymer blends containing core-shell-type impact modifiers. In the present study, further experiments have been carried out on ABS polymers with different rubber content to verify the validity of our linear viscoelastic model. It has been found that our model describes quite properly the rheological behavior of ABS polymers with different rubber content, especially at low frequencies. The experimental data confirm that our model describes the rheological properties of rubber-modified thermoplastic polymers with strong adhesion at the particle/matrix interface more accurately than the Palierne model.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.641-644
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• 2005

The study on the interaction forces of some biological materials is important to understanding biological phenomena and their application to practical purpose. This paper introduces a measuring technique for biological adhesive forces using the AFM(Atomic Force Microscope). Since no standardized thesis on adhesive forces exist, the adhesive forces is defined as adhesive forces against a hardened surface of biological materials. To grant the results are meaningful, which is based on the understanding the surface characteristics of biological materials using the AFM, a nominal value of average adhesive force per unit area should be measured. Therefore the modified AFM probe with small micro glass bead was proposed so that it can guarantee the required contact area for measuring the average adhesive forces. A pyrex glass substrate with circular patterns, which was fabricated by micromachining technique, is introduced in order to controll the contact area. The two types of mussel adhesive proteins, Celltak and recombinant-MGFP5, were tested by the proposed measuring method. The test results show that the adhesive force of the mussel adhesive proteins can be reliably measured by use of this method.

• Journal of KSNVE
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• v.8 no.6
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• pp.1093-1102
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• 1998

The success of controllability of smart structures depends on the quality of the bonding along the interface between the main structure and the attached sensing and acuating elements. Generally, the analysis procedures neglect the effect of the interfacial bond layer or assume that this bond layer behaves like viscoelastic material. Three different bond layers. two modified epoxy adhesives, and one isocyanate adhesive were prepared for their toughness and moduli. Bond layer of the chosen adhesive provides an almost perfect bonding condition between the composite structure and the PZT while bended significantly like arrow-shape. The perfect bonding condition is tested by considering various material properties of the bond layers. and based on this perfect bonding condition, the effects of the interfacial bond layer on the dynamic behavior and controllability of the test structure is experimentally studied. Once the perfect bonding condition is achieved. dynamic effects of the bond layer itself on the dynamic characteristics of the main structure is negligible. but the contribution of the attached PZT elements on the stiffness of the multi-layered structure becomes significant when the thickness of the bond layer increased.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.10a
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• pp.15-20
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• 2005

Locations of silicon accumulation in rice leaves and its possible association with resistance to rice blast were investigated by analytical electron microscopy and atomic force microscopy. A blast-susceptible cultivar, Jinmi, and partially resistant cultivars, Hwaseong and Suwon345, were grown under a hydroponic culture system with modified Yoshida's nutrient solution. Electron-dense silicon layers were frequently found beneath the cuticle in epidermal cell walls of silicon-treated plants. Increasing levels of silicon were detected in the outer regions of epidermal cell walls. Silicon was present mainly in epidermal cell walls, middle lamella, and Intercellular spaces within subepidermal tissues. Furthermore, silicon was prevalent throughout the leaf surface with relatively small deposition on stomatal guard cells in silicon-treated plants. Force-distance curve measurements revealed relative hardness and smaller adhesion force in silicon-treated plants (18.65 uN) than control plants (28.39 uN). Moreover, force modulation microscopy showed higher mean height values of elastic Images In silicon-treated plants(1.26 V) than in control plants (0.44 V), implying the increased leaf hardness by silicon treatment. These results strongly suggest that silicon-induced cell wall fortification of rice leaves may be closely associated with enhanced host resistance to blast.

• Biomaterials and Biomechanics in Bioengineering
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• v.1 no.3
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• pp.163-174
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• 2014

The objective of this study is to prepare an artificial extracellular matrix (ECM) for cell culture by using polymer hydrogels. The polymer used is a cytocompatible water-soluble phospholipid polymer: poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-n-butyl methacrylate-p-nitrophenyloxycarbonyl poly(ethylene oxide) methacrylate (MEONP)] (PMBN). The hydrogels are prepared using a cross-linking reaction between PMBN and diamine compounds, which can easily react to the MEONP moiety under mild conditions. The most favorable diamine is the bis(3-aminopropyl) poly(ethylene oxide) (APEO). The effects of cross-linking density and the chemical structure of cross-linking molecules on the mechanical properties of the hydrogel are evaluated. The storage modulus of the hydrogel is tailored by tuning the PMBN concentration and the MEONP/amino group ratio. The porous structure of the hydrogel networks depends not only on these parameters but also on the reaction temperature. We prepare a hydrogel with $40-50{\mu}m$ diameter pores and more than 90 wt% swelling. The permeation of proteins through the hydrogel increases dramatically with an increase in pore size. To induce cell adhesion, the cell-attaching oligopeptide, RGDS, is immobilized onto the hydrogel using MEONP residue. Bovine pulmonary artery endothelial cells (BPAECs) are cultured on the hydrogel matrix and are able to migrate into the artificial matrix. Hence, the RGDS-modified PMBN hydrogel matrix with cross-linked APEO functions as an artificial ECM for growing cells for applications in tissue engineering.

• Proceedings of the KOSOMBE Conference
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• v.1989 no.05
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• pp.5-6
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• 1989

Polyurethane surface was chemically modified to have different hydrophilic polyethyleneoxide(PEO)/hydrophobic dodecanediol(DDO) groups and negatively charged sulfonate group to investigate the effect to the antithrombogenicity. The hydrophilicity of the surface was significantly increased after PEO grafting or sulfonation. Lowering in-vitro platelet adhesion led to a prologation in the ex-vivo occlusion time. Especially, the sulfonated PU-PEO surface showed most enhanced blood compatibility due to the synergistic effects of PEO and $SO_3$ groups.