• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.775-784
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• 2017

A neutral xylanase (CcXyn) was identified from Coprinus cinereus. It has a single GH10 catalytic domain with a basic amino acid-rich extension (PVRRK) at the C-terminus. In this study, the wild-type (CcXyn) and C-terminus-truncated xylanase ($CcXyn-{\Delta}5C$) were heterologously expressed in Pichia pastoris and their characteristics were comparatively analyzed with aims to examine the effect of this extension on the enzyme function. The circular dichorism analysis indicated that both enzymes in general had a similar structure, but $CcXyn-{\Delta}5C$ contained less ${\alpha}-helices$ (42.9%) and more random coil contents (35.5%) than CcXyn (47.0% and 32.8%, respectively). Both enzymes had the same pH (7.0) and temperature ($45^{\circ}C$) optima, and similar substrate specificity on different xylans. They all hydrolyzed beechwood xylan primarily to xylobiose and xylotriose. The amounts of xylobiose and xylotriose accounted for 91.5% and 92.2% (w/w) of total xylooligosaccharides (XOS) generated from beechwood by CcXyn and $CcXyn-{\Delta}5C$, respectively. However, truncation of the C-terminal 5-amino-acids extension significantly improved the thermostability, SDS resistance, and pH stability at pH 6.0-9.0. Furthermore, $CcXyn-{\Delta}5C$ exhibited a much lower $K_m$ value than CcXyn (0.27 mg/ml vs 0.83 mg/ml), and therefore, the catalytic efficiency of $CcXyn-{\Delta}5C$ was 2.4-times higher than that of CcXyn. These properties make $CcXyn-{\Delta}5C$ a good model for the structure-function study of $({\alpha}/{\beta})_8$-barrel-folded enzymes and a promising candidate for various applications, especially in the detergent industry and XOS production.

• Journal of computational fluids engineering
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• v.21 no.2
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• pp.112-119
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• 2016

NEXTSat-1 is the next-generation small-size artificial satellite system planed by the Satellite Technology Research Center(SatTReC) in Korea Advanced Institute of Science and Technology(KAIST). For the control of attitude and transition of the orbit, the system has adopted a RHM(Resisto-jet Head Module), which has a very simple geometry with a reasonable efficiency. An axisymmetric model is devised with two coil-resistance heaters using xenon(Xe) gas, and the minimum required specific impulse is 60 seconds under the thrust more than 30 milli-Newton. To design the module, seven basic parameters should be decided: the nozzle shape, the power distribution of heater, the pressure drop of filter, the diameter of nozzle throat, the slant length and the angle of nozzle, and the size of reservoir, etc. After quasi one-dimensional analysis, a theoretical value of specific impulse is calculated, and the optima of parameters are found out from the baseline with a series of multi-physical numerical simulations based on the compressible Navier-Stokes equations for gas and the heat conduction energy equation for solid. A commercial code, COMSOL Multiphysics is used for the computation with a FEM (finite element method) based numerical scheme. The final values of design parameters indicate 5.8% better performance than those of baseline design after the verification with all the tuned parameters. The present method should be effective to reduce the time cost of trial and error in the development of RHM, the thruster of NEXTSat-1.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.4
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• pp.353-360
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• 2015

Commercial drivers feel tired more than the general public, because their driving times are long and they experience more idle vibration. In this study, we developed a nonlinear model of a magnetic, linear spring seat suspension to determine the optimal design to improve ride comfort. The resonant frequency for the optimal design of the suspension was found to be 3.5 Hz, and the stiffness was analyzed through displacement-load experiments. Additionally, the vibration transmissibility was analyzed by the suspension stiffness, and the existing coil spring type vibration transmissibility was found to be 0.99. A parallelogram type magnetic spring was determined to result in a better performance than the existing spring with a vibration transmissibility of 0.823.

• Smart Structures and Systems
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• v.14 no.5
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• pp.901-916
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• 2014

Magnetorheological (MR) fluid is one of the field-responsive fluids that is of interest to many researchers due to its high yield stress value, which depends on the magnetic field strength. Similar to electrorheological (ER) fluid, the combination of working modes is one of the techniques to increase the performance of the fluids with limited focus on MR fluids. In this paper, a novel MR testing cell incorporated with valve, shear and squeeze operational modes is designed and constructed in order to investigate the behaviour of MR fluid in combined mode. The magnetic field distribution in the design concept was analyzed using finite element method in order to verify the effective areas of each mode have the acceptable range of flux density. The annular gap of valve and shear were fixed at 1 mm, while the squeeze gap between the parallel circular surfaces was varied up to 20 mm. Three different coil configurations, which were made up from 23 SWG copper wires were set up in the MR cell. The simulation results indicated that the magnetic field distributed in the squeeze gap was the highest among the other gaps with all coils were subjected to a constant applied current of 1 A. Moreover, the magnetic flux densities in all gaps were in a good range of magnitude based on the simulations that validated the proposed design concept. Hence, the 3D model of the MR testing cell was designed using Solidworks for manufacturing processes.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.3
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• pp.209-217
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• 2013

This paper presents an optimal design of a magnetorheological(MR) fluid-based mount(MR mount) that can be used for to vibration control in diesel engines of ships. In this work, a mount that uses mixed-modes(squeeze mode, flow mode, and shear mode) is proposed and designed. To determine the actuating damping force of the MR mount required for efficient vibration control, the excitation force from a diesel engine is analyzed. In this analysis, a model of a V-type engine is considered. The relationship between the velocity and pressure of gas in terms of the torque acting on the piston is derived. Subsequently, by integrating the field-dependent rheological properties of commercially available MR fluid with the excitation force, the appropriate size of the MR mount is designed. In addition, to achieve the maximum actuating force under geometric constraints, design optimization is undertaken using the ANSYS parametric design language software. Through magnetic density analysis, optimal design parameters such as the bottom gap and radius of coil are determined.

• Progress in Superconductivity and Cryogenics
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• v.15 no.2
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• pp.20-23
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• 2013

For sensitive measurements of micro-Tesla nuclear magnetic resonance (${\mu}T$-NMR) signal, a low-noise superconducting quantum interference device (SQUID) system is needed. We have fabricated a liquid He dewar for an SQUID having a large diameter for the pickup coil. The initial test of the SQUID system showed much higher low-frequency magnetic noise caused by the thermal magnetic noise of the aluminum plates used for the vapor-cooled thermal shield material. The frequency dependence of the noise spectrum showed that the noise increases with the decrease of frequency. This behavior could be explained from a two-layer model; one generating the thermal noise and the other one shielding the thermal noise by eddy-current shielding. And the eddy-current shielding effect is strongly dependent on the frequency through the skin-depth. To minimize the loop size for the fluctuating thermal noise current, we changed the thermal shield material into insulated thin Cu mesh. The magnetic noise of the SQUID system became flat down to 0.1 Hz with a white noise of 0.3 $fT/{\surd}Hz$, including the other noise contributions such as SQUID electronics and magnetically shielded room, etc, which is acceptable for low-noise ${\mu}T$-NMR experiments.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.20 no.12
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• pp.1128-1138
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• 2010

One of the typical problems in the precise vibration is resonance characteristics at low frequency disturbance due to a heavy mass. An electro-magnetic(EM) air spring is a kind of vibration control unit and active isolator. The EM air spring in this study aims at removing the low frequency resonance for semiconductor manufacturing. The mechanical and electronic parts in the active isolator are designed to operate under a weight of 2.5 tons. The EM spring is floated using air pressure in a pneumatic elastic chamber and actuated by EM levitation force. The actuator consists of a EM coil and a permanent magnetic plate which are installed inside of the chamber. An air mount was constructed for the experiment with a stone surface plate, 4 active air springs, 4 gap sensors, a DSP controller, and a multi-channel power amp. A PD control method and operating logic was applied to the DSP. Simulation using 1/4 model was carried out and compared with the experiments. The time duration and maximum peak at resonance frequency can be reduced sharply by the proposed system. The results show that the active system can avoid the resonance caused by the natural frequency of the passive system.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2012.10a
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• pp.93-99
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• 2012

This paper presents an optimal design of magnetorheological (MR) fluid based mount (MR mount in short) which can be applicable to vibration control of diesel engine of ship. In this work, a mixed - mode including squeeze mode, flow mode and shear mode is proposed and designed. In order to determine actuating damping force of MR mount required for efficient vibration control, excitation force from diesel engine is analyzed. In this analysis, a model of V-type engine is considered and the relationship between velocity and pressure of gas in torque of the piston is derived. Subsequently, by integrating the field-dependent rheological properties of commercially available MR fluid with the excitation force an appropriate size of MR mount is designed. In addition, in order to achieve maximum actuating force with geometric constraints design optimization is undertaken using ANSYS software. Through the magnetic density analysis, optimal design parameters such as bottom gap and radius of coil are determined.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.10
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• pp.3858-3874
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• 2021

As an essential part of the urban transportation system, precise perception of the traffic flow parameters at the traffic signal intersection ensures traffic safety and fully improves the intersection's capacity. Traditional detection methods of road traffic flow parameter can be divided into the micro and the macro. The microscopic detection methods include geomagnetic induction coil technology, aerial detection technology based on the unmanned aerial vehicles (UAV) and camera video detection technology based on the fixed scene. The macroscopic detection methods include floating car data analysis technology. All the above methods have their advantages and disadvantages. Recently, indoor location methods based on wireless signals have attracted wide attention due to their applicability and low cost. This paper extends the wireless signal indoor location method to the outdoor intersection scene for traffic flow parameter estimation. In this paper, the detection scene is constructed at the intersection based on the received signal strength indication (RSSI) ranging technology extracted from the wireless signal. We extracted the RSSI data from the wireless signals sent to the road side unit (RSU) by the vehicle nodes, calibrated the RSSI ranging model, and finally obtained the traffic flow parameters of the intersection entrance road. We measured the average speed of traffic flow through multiple simulation experiments, the trajectory of traffic flow, and the spatiotemporal map at a single intersection inlet. Finally, we obtained the queue length of the inlet lane at the intersection. The simulation results of the experiment show that the RSSI ranging positioning method based on wireless signals can accurately estimate the traffic flow parameters at the intersection, which also provides a foundation for accurately estimating the traffic flow state in the future era of the Internet of Vehicles.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.05a
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• pp.584-588
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• 2021

The impedance of the speaker is likely to be recognized as a fixed value. However, speaker impedance continues to vary with frequency variation, especially larger in resonant frequency region. The sound pressure level of loudspeakers is determined by the current flowing throughout the coil that consists loudspeakers. If loudspeakers are driven by voltage, sound pressure level of the loudspeaker is distorted by the variation of loudspeaker impedance. Current-drive of loudspeakers can solve this problem, but distortion of sound pressure level occurs in low frequencies due to resonance. The distortion can degrade the sound quality of the sound system. So to solve this problem, In this paper, we propose a resonance compensation circuit using DSP. we simulates audio systems using an equivalent model of loudspeakers to verify distortion of sound pressure level due to impedance variation and propose a circuit to compensate it. The proposed circuit is configured using a state variable filter and it can adjust the center frequency and output, so it will be used various sound systems.