• Applied Biological Chemistry
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• v.38 no.4
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• pp.313-319
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• 1995

The involvement of the cell-wall degrading enzymes in Rhizobium has long been an unsolved question about the infection process in the formation of root nodule. To assess the contribution of the cellulase to the nodulation of rhzobia, here we report the production of cellulase from R. meliloti TAL1372 which degrade carboxymethylcellulose (CMC) model substrate with CMC-plate method. We constructed a genomic library by cloning Sau3A-digested genomic DNA from R. meliloti TAL1372 into the BamHI site of the cosmid vector pLAFR3. Out of more than one thousand transductants of E. coli, one clone (pRC8-71) had CM-cellulase activity and contained pLAFR3 cosmid with 30 kb insert of R. meliloti DNA The product of CM-cellulase gene was analyzed by native PAGE. About 45 kD protein was considered to be a product of the gene. Tn5 mutagenesis reveals that the structural gene located in a ca. 3 kb KpnI fragment. The cellulase-minus mutants of R. meliloti TAL1372 were obtained by Tn5 mutagenesis of pRC8-71 and marker exchange techniques. Analyses of the nodulation ability of these Tn5 mutants showed that the CM-cellulase gene of R. meliloti TAL1372 may be involved in early nodulation development on alfalfa (Medicago satiua).

• Journal of the Korean Society for information Management
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• v.37 no.3
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• pp.301-326
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• 2020

This study analyzed recent cases of transition to open access(OA) journals in humanities and social sciences(HSS) in foreign countries to find a way to transition to OA journals in Korea. Ling OA and UP led by researchers, OLH and KU led by libraries, S2O led by publishers and L+F model led by funders and libraries were analyzed. Programs by national funding agencies such as the Norwegian Research Council, Canada Social Sciences and Humanities Research Council, Austrian Science Fund, and Swiss Academy of Humanities and Social Sciences were also reviewed. Based on the results of the analyses, this study suggests for transition in Korean HSS field as follows; 1) transitions to open access journals in HSS should be supported at national level 2) the stakeholder in scholarly communications such as the nation, universities, libraries, research funders, scholarly societies etc has their own role in the transition but funders and libraries are the most important in financial support for open access publishing 3) but the financial support for the first stage of the transition should be done at national level 4) and open access journal publishing consortium comprised of libraries and information service institutions in the public sector such as KERIS, KISTI and the national library should be established 5) non commercial publishing platforms for scholarly societies should be developed and distributed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.1
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• pp.46-54
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• 1997

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by malting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB (spo 5)1. We futher analyzed six recombinant plasmids, pDB (spo 5)2, pDB(spo 5)3, pDB(spo 5)4, pDB(spo 5)5, pDB(spo 5)6, pDB(spo 5)7, and found each plasmids is able to rescue the spo 5-2, spo 5-3, spo 5-4, spo 5-5, spo 5-6, spo 5-7, mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB (spo 5)1, and pDB (spo 5)R1 contained the spo 5 gene. Transcipts of spo 5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 25 kb were detected with 5 kb Hind III fragment containing a part of the spo 5 gene as a probe. The small mRNA (2.5 kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2 kb) was produced constitutively. Appearance of a 2.5 kb spo 5-mRNA depends upon the function of the mei1, mei2 and mei3 genes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.271-284
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• 2016

This study was carried out to evaluate the efficacy of CBT for perinatal depression through systematic literature review and meta-analysis. The following databases were used to search the literature: CINAHL, PubMed, EMBASE, Koreamed, Library of Korean Congress, KISS, and Korean Academic Publication Database. Keywords included 'perinatal depression,' 'pregnant women,' and 'cognitive behavioral therapy,' and the evaluated articles were published up to May 2016. Using the R program, the effect size of perinatal depression and anxiety were calculated by random-effects model. The heterogeneity of the effect size was analyzed by data moderator analysis using the meta-ANOVA. Furthermore, the funnel plot, Egger's regression test, fail-safe N, trim-and-fill test, and publication bias analysis were conducted and used to verify the results. Out of the 180 selected articles, 16 clinical trial studies were meta-analyzed. Each articles were evaluated for the risk of bias by the checklist of SIGN; the overall risk of bias was low. The effect size of CBT for perinatal depression was Hedges' g=-0.55 (95% CI: -0.76~-0.33), which was a moderate level, while for anxiety reduction, Hedges' g=-0.20 (95% CI: -0.48~-0.08) and it was not statistically significant. Heterogeneity or risk of publication bias were low. This meta-analytic study found that CBT is moderately effective in reducing perinatal depression in pregnant women.

• Journal of The Korean Association of Information Education
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• v.12 no.4
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• pp.437-448
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• 2008

As the importance of knowledge is emphasized and the environment of battlefields is changing, the military also demands competent people equipped with creativity, cooperativeness and communication ability, and in this situation it is required to apply PBL to education in the navy. The present study went through three stages in order to develop a prototype to implement a naval e PBL support system for knowledge creation. First, databases in Korea Education and Research Information Service, National Assembly Library, etc. were searched using keywords such as PBL, e-PBL, knowledge creation and knowledge ecosystem. In addition, we selected and analyzed frequently quoted literature and recent research reports related to this study among domestic and foreign theses, books, research papers, etc. recommended by specialists in contents, and derived the key values of a knowledge creation type e-PBL support system and design strategies. Second, we developed a primary prototype based on the contents of analysis and, revising it according to teaching design specialists' opinions, we proposed the final prototype of knowledge creation type naval e PBL support system and it has values as follows. First, the knowledge creation type naval e PBL support system provides learners with opportunities to apply e PBL and helps them improve their creativity, cooperativeness and communication ability and accumulate know how of services. Second, it improves work efficiency by circulating knowledge through sharing among individuals or groups, and produces synergy that promotes the organizational culture of learning. Third, the knowledge creation type naval e-PBL support system enables teachers who apply PBL to school education to find new applications of PBL in constructing knowledge bases.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.1A
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• pp.93-98
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• 2004

Serial ATA interface Is inexpensive comparatively and performance is superior. So it is suitable technology in demand that now require data transmission and throughput of high speed. This paper describes a design and implementation of Serial ATA Link layer about error detection and 8b/10b encoder/decoder for DC balance in frequency 150MHz. The 8b/10b Encoder is partitioned into a 5b/6b plus a 3b/4b coder. The logical model of the block is described by using Verilog HDL at register transistor level and the verified HDL is synthesized using standard cell libraries. And it is fabricated with $0.35{\mu}m$ Standard CMOS Cell library and the chip size is about $1500{\mu}m\;*\;1500{\mu}m$. The function of this chip has been verified and tested using testboard with FPGA equipment and IDEC ATS2 test equipment. It is used to frequency of 100MHz in verification processes and supply voltage 3.3V. The result of testing is well on the system clock 100MHz. The designed and verified each blocks may be used IP in the field of high speed serial data communication.

• Molecules and Cells
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• v.28 no.6
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• pp.529-536
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• 2009

Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5' ends of five full-length enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5′ ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.7
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• pp.37-47
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• 2011

Real picture like high quality computer graphic is widely used in various fields and shader processor, a key part of a graphic processor, has been advanced to programmable unified shader. However, The existing graphic processors have been optimized to commercial algorithms, so development of an algorithm which is not based on it requires an independent shader processor. In this paper, we have designed and implemented a control unit to support high quality 3 dimensional computer graphic image on programmable integrated shader processor. We have done evaluation through functional level simulation of designed control unit. Hardware resource usage rate are measured by implementing directly on FPGA Virtex-4 and execution speed are verified by applying ASIC library. the result of an evaluation shows that the control unit has the commands more about 1.5 times compared to the other shader processors that is a behavior similar to the control unit and with a number of processing units used in a shader processor, compared with the other processors, overall performance of the control unit is improved about 3.1 GFLOPS.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.10
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• pp.72-81
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• 2011

In this paper, we proposed and designed a novel floating point converter which supports single and double precisions of IEEE754 standard. The proposed convertor supports conversions between floating point number single/double precision and signed fixed point number(32bits/64bits) as well as conversions between signed integer(32bits/64bits) and floating point number single/double precision and conversions between floating point number single and double precisions. We defined a new internal format to convert various input types into one type so that overflow checking could be conducted easily according to range of output types. The internal format is similar to the extended format of floating point double precision defined in IEEE754 2008 standard. This standard specifies that minimum exponent bit-width of the extended format of floating point double precision is 15bits, but 11bits are enough to implement the proposed converting unit. Also, we optimized rounding stage of the convertor unit so that we could make it possible to operate rounding and represent correct negative numbers using an incrementer instead an adder. We designed single cycle data path and 5 cycles data path. After describing the HDL model for two data paths of the convertor, we synthesized them with TSMC 180nm technology library using Synopsys design compiler. Cell area of synthesis result occupies 12,886 gates(2 input NAND gate), and maximum operating frequency is 411MHz.

• Korean Journal of Cognitive Science
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• v.19 no.4
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• pp.419-441
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• 2008

As the Web and digital libraries have become a commodity, they are used for a variety of purposes and tasks that may require a great deal of cognitive efforts. However, most search engines in the Web and digital libraries support users with only searching and browsing capabilities, leaving all the cognitive burdens of manipulating information objects to the users. We propose a two-level model for human-Web interactions, consisting of knowledge and information spaces, and a tool that provides knowledge space and inter-space operations in addition to searching and browsing at the information level. Knowledge space is an explication of user's conceptual view of the information objects being explored through interactions with the Web or a digital library. Topics are created and related with associations at the knowledge level and connected to information objects in information space. The tool implemented using the Topic Maps framework has been tested for efficacy as an aid to reducing cognitive burden under exploratory search task.