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Oral tissue response to soft tissue expanders prior to bone augmentation: in vitro analysis and histological study in dogs

  • Yoo, Jung Min;Amara, Heithem Ben;Kim, Min Kyoung;Song, Ju Dong;Koo, Ki-Tae
    • Journal of Periodontal and Implant Science
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    • v.48 no.3
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    • pp.152-163
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    • 2018
  • Purpose: To determine whether the swelling and mechanical properties of osmotic self-inflating expanders allow or not the induction of intraoral soft tissue expansion in dogs. Methods: Three different volumes (0.15, 0.25, and 0.42 mL; referred to respectively as the S, M, and L groups) of soft tissue expanders (STEs) consisting of a hydrogel core coated with a silicone-perforated membrane were investigated in vitro to assess their swelling behavior (volume swelling ratio) and mechanical properties (tensile strength, tensile strain). For in vivo investigations, the STEs were subperiosteally inserted for 4 weeks in dogs (n=5). Soft tissue expansion was clinically monitored. Histological analyses included the examination of alveolar bone underneath the expanders and thickness measurements of the surrounding fibrous capsule. Results: The volume swelling ratio of all STEs did not exceed 5.2. In tensile mode, the highest mean strain was registered for the L group ($98.03{\pm}0.3g/cm$), whereas the lowest mean value was obtained in the S group ($81.3{\pm}0.1g/cm$), which was a statistically significant difference (P<0.05). In addition, the S and L groups were significantly different in terms of tensile strength ($1.5{\pm}0.1g/cm$ for the S group and $2.2{\pm}0.1g/cm$ for the L group, P<0.05). Clinical monitoring showed successful dilatation of the soft tissues without signs of inflammation up to 28 days. The STEs remained volumetrically stable, with a mean diameter in vivo of 6.98 mm, close to the in vitro post-expansion findings (6.69 mm). Significant histological effects included highly vascularized collagen-rich fibrous encapsulation of the STEs, with a mean thickness of $0.67{\pm}0.12mm$. The bone reaction consisted of resorption underneath the STEs, while apposition was observed at their edges. Conclusions: The swelling and mechanical properties of the STEs enabled clinically successful soft tissue expansion. A tissue reaction consisting of fibrous capsule formation and bone loss were the main histological events.

Effect of surface treatment on shear bond strength between artificial resin teeth and 3D printing denture base resin (인공치의 표면처리가 3D 프린팅 의치상레진과의 전단결합강도에 미치는 영향)

  • Choi, Jeehye;Lee, Younghoo;Hong, Seoung-jin;Paek, Janghyun;Noh, Kwantae;Pae, Ahran;Kwon, Kung-Rock;Kim, Hyeong-Seob
    • The Journal of Korean Academy of Prosthodontics
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    • v.58 no.4
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    • pp.300-305
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    • 2020
  • Purpose: The purpose of this study is to compare the shear bond strength of 3D printing denture base resin according to surface treatment of artificial teeth. Materials and methods: 3D printing denture base resin was fabricated as specimens using 3D printer. The experimental group divided the surface treatment of artificial teeth into five groups according to the application of sandblasting and primer (n=10). Shear bond strengths between denture base and artificial teeth were measured by universal testing machine. All measurements were analyzed by one-way ANOVA and Turkey test (α=.05). Fracture mode of each specimen was analyzed. Microscopic evaluation was conducted by using a scanning electron microscope. Results: Unsurfaced treated group represented the lowest value. The primer groups had significantly higher result values (P<.05). Most specimens of the primer groups had cohesive failure. Conclusion: In 3D printing denture base resin group, mechanical and chemical surface treatment of artificial teeth has increased the shear bond strength. Therefore, if dentures are produced using 3D printing, proper mechanical and chemical treatment of artificial teeth is necessary for adhesion of dentures and artificial teeth.

A Study on the utilizing parody and pastiche in Contemporary Art Works (현대 예술 작품에서 나타나는 패러디와 패스티쉬에 관한 연구)

  • Song, Ho-Jin;Jeong, Eui-Tae
    • Journal of the Korea Convergence Society
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    • v.6 no.6
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    • pp.201-212
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    • 2015
  • They insist that there is no more new stuff in modern art to change every circumstance rapidly. According to this situation, they recreate the works which are made with the existed idea or materials, and also transform creative concept which cites the existing functions with modern meaning. Furthermore they can look into any data everywhere from their daily routine using the internet. And this must become one of the general forms which they imitate others' works which are referred or chosen in modern art. These social complextion is called a parody and pastich is technique of the Post Modernism which is the existence of past. Using the new construction, the parody and pastich which can accomplish some better value and bring other meanings makes original recreation with the concept of copying and repeating. It keeps changing and is shaping up to be different aspect so far. Every field has a little different term using its own expression but it keeps empathizing each other a same essential meaning. Although it is like universal mode of expression, It is fact that the public cannot distinguish what is creation, imitation, or piracy. This thesis shows the concept of it and files for terms which is using like that. At last it selects some artist who worked with parody and pastich analyses and compares them and their works.

The Analysis of Competition Structure in Business Data Service Market Using Henry Model and Suggestion for Competitive Strategies (Hendry Model을 활용한 기업용데이터서비스시장의 경쟁구조 분석 및 전략 제언)

  • 유광숙;최문기
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.12C
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    • pp.280-291
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    • 2001
  • LL (Leased Line service) is a facility-based service as a traditional business data service, but new competition services, such as FR (Frame Relay), VPN (Virtual Private Network), and ATM (Asynchronous Transfer Mode), are value-added services. Because of different service classifications, it is hard to gather necessary data for the service providers to plan their market strategies and regulations and policies are also applied asymmetrically to each service provider. Therefore an appropriate market classification is required for the business data services. After various methods of market classification are reviewed, the Hendry model is selected in this paper to analyze substitution-degree among brands or among services. Since the structure of virtual competitions is required for the Hendry model to be applied to data service market, the market is analyzed first by the well-known Porter's model. By the analysis of Porter's model, two virtual competition structures are set up - one is for the competitions among leased line service providers, and the other is for the competitions among business data services such as LL, FR, VPN and ATM. After the Hendry model is applied to each competition structure, it is confirmed that 7 LL service providers do not compete directly, but 2 sub-markets exist for the LL service provisions. However, it is shown that 4 business data services compete directly. Using the Switching Probability Matrix from Hendry model, future market shares of LL service providers and market shares of business data services are forecasted. These empirical results are helpful for service providers to set competitive strategies with the minimization of cannibalization effect and they can easily and efficiently predict their market demands.

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A STUDY ON THE TENSILE STRENGTH OF REINFORCED VENEERING COMPOSITE RESINS FOR CROWN (강화형 치관용 복합레진의 인장강도에 관한 연구)

  • Ahn, Seung-Geun;Kang, Dong-Wan
    • The Journal of Korean Academy of Prosthodontics
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    • v.38 no.2
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    • pp.226-241
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    • 2000
  • Recently a new generation of crown and bridge veneering resins containing submicron glass fillers was introduced. These ultrasmall particle hybrid composite materials distinguish themselves, compared with conventional microfill crown and bridge resins, through improved mechanical properties. It is claimed that these composites are suitable for metal free crowns and even bridges using fiber reinforcement. The purpose of this study was to evaluate the effect of thermal cycling on the tensile strength of the following veneering composites: Artglass(Heraeus Kulzer Co., Wehrheim, Germany), Estonia(Kuraray Co.. Japan), Sculpture(Jeneric Pentron Co., Wallingford, U.S.A.), and Targis(Ivoclar Co., Schaan Liechenstein). According to manufacturer's instructions, rectangular tensile test specimens measuring $1.5{\times}2.0{\times}4.5mm$ were made using a teflon mold. Whole specimens were divided into two groups. One group was dried in a desiccator at $25^{\circ}C$ for 10 days, and another group was subjected to thermal cycling($10,000{\times}$) in water($5/55^{\circ}C$). All test specimens were placed in a universal testing machine and loaded until fracture with a crosshead speed of 0.5mm/min. Weibull analysis and Tukey's test were used to analyze the data. The fracture surfaces of specimens were observed in SEM and the aliphatic C=C absorbance peak of Estenia and Targis resin was analyzed using Fourier transform infrared(FTIR) spectroscopy. Within the limitations imposed in this study, the following conclusions can be drawn: 1. Both in drying condition and thermal cycling condition, the highest tensile strength was observed in Estenia testing group(p<0.05). 2. The strength data were at to single-mode Weibull distribution, and the Weibull modulus of all veneering composite resin specimens increased after thermal cycling treatment. 3. After thermal cycling test, the highest tensile strength was observed in the Estenia group, and the lowest value was observed in the Targis group. The tensile strength values showed the significant differences between each group(p<0.05) 4. The aliphatic C=C absorbance peak of Estonia and Targis resin was decreased after light curing, and there was no distinct change after thermal cycling.

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Production of a Monoclonal Antibody to Human $\alpha$-Fetopotein and Development of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays for Human $\alpha$-Fetoprotein (인간 $\alpha$-fetoprotein에 대한 모노클로날 항체의 제조 및 모노클로날 항체를 이용한 효소면역분석법의 개발)

  • Michung Yoon;Hyun-Hee Lee;Youngwon Lee
    • Biomedical Science Letters
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    • v.5 no.1
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    • pp.1-10
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    • 1999
  • This study was attempted to generate a monoclonal antibody against human $\alpha$-fetoprotein (AFP) and to produce an immunoassay, recognizing AFP in plasma and amniotic fluid. AFP was purified from human amniotic fluid and used to immunize mice. Spleens were taken from the mice and the cells were fused with mouse myeloma cells (Sp2/0-Ag-14) for the production of monoclonal antibodies by employing the hybridoma technology. As a result, a hybridoma cell line producing anti-AFP monoclonal antibody was cloned out and designated as MabF22. From isotyping analysis, it was found that monoclonal antibody MabF22 was IgG type with IgG1 heavy chain and k light chain. The binding specificity of MabF22 was analyzed by immunoblotting as well as by ELISA. MabF22 was highly specific, reacting with only AFP-containing samples. The binding affinity was determined by ELISA (free-capture mode) and Scatchard analysis. As a result, the value of Kd was 0.8$\times$10$^{-10}$M. The validity of the MabF22 for AFP assay was examined by two kinds of ELISAs, i.e., non-competitive and competitive ELISA. Both assays revealed that MabF22 reacted well with AFP in sample in a concentration-dependent manner. Standard curve and antibody titration curve were obtained by using purified AFP and MabF22. These results indicate that the monoclonal antibody produced in this study would be useful not only for research purposes but also for further development of immune-diagnostic kit for the measurement of AEP concentration.

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Two Dimensional Size Effect on the Compressive Strength of T300/924C Carbon/Epoxy Composite Plates Considering Influence of an Anti-buckling Device (T300/924C 탄소섬유/에폭시 복합재 적층판의 이차원 압축 강도의 크기효과 및 좌굴방지장치의 영향)

  • ;;;C. Soutis
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2002.10a
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    • pp.88-91
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    • 2002
  • The two dimensional size effect of specimen gauge section (length x width) was investigated on the compressive behavior of a T300/924 [45/-45/0/90]3s, carbon fiber-epoxy laminate. A modified ICSTM compression test fixture was used together with an anti-buckling device to test 3mm thick specimens with a 30$\times$30, 50$\times$50, 70$\times$70, and 90mm$\times$90mm gauge length by width section. In all cases failure was sudden and occurred mainly within the gauge length. Post failure examination suggests that $0^{\circ}$ fiber microbuckling is the critical damage mechanism that causes final failure. This is the matrix dominated failure mode and its triggering depends very much on initial fiber waviness. It is suggested that manufacturing process and quality may play a significant role in determining the compressive strength. When the anti-buckling device was used on specimens, it was showed that the compressive strength with the device was slightly greater than that without the device due to surface friction between the specimen and the device by pretoque in bolts of the device. In the analysis result on influence of the anti-buckling device using the finite element method, it was found that the compressive strength with the anti-buckling device by loaded bolts was about 7% higher than actual compressive strength. Additionally, compressive tests on specimen with an open hole were performed. The local stress concentration arising from the hole dominates the strength of the laminate rather than the stresses in the bulk of the material. It is observed that the remote failure stress decreases with increasing hole size and specimen width but is generally well above the value one might predict from the elastic stress concentration factor. This suggests that the material is not ideally brittle and some stress relief occurs around the hole. X-ray radiography reveals that damage in the form of fiber microbuckling and delamination initiates at the edge of the hole at approximately 80% of the failure load and extends stably under increasing load before becoming unstable at a critical length of 2-3mm (depends on specimen geometry). This damage growth and failure are analysed by a linear cohesive zone model. Using the independently measured laminate parameters of unnotched compressive strength and in-plane fracture toughness the model predicts successfully the notched strength as a function of hole size and width.

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Applying Evaluation of Soil Erosion Models for Burnt Hillslopes - RUSLE, WEPP and SEMMA (산불사면에 대한 토양침식모형의 적용 평가 - RUSLE, WEPP, SEMMA)

  • Park, Sang Deog;Shin, Seung Sook
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.31 no.3B
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    • pp.221-232
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    • 2011
  • Applicability of three soil erosion models for burnt hillslopes was evaluated. The models were estimated with the data from plots established after tremendous wildfire occurred in the east coastal region. Soil erosion and surface runoff were simulated by the Water Erosion Prediction Project (WEPP) and the Revised Universal Soil Loss Equation (RUSLE) of application mode for disturbed forest areas and the Soil Erosion Model for Mountain Areas (SEMMA) developed for burnt hillslopes. Simulated sediment yield and surface runoff were compared with the measured those. In maximum value of sediment yield, three models was under-predicted and RUSLE and WEPP had difference of over two times. SEMMA showed the best model response coefficient, determination coefficient and the model efficiency. In application of models to the soil erosion according to the elapsed year after wildfire, all models were underestimated in initial stage disturbed by wildfire. Evaluation of models in this burnt hillslopes was shown the tends to under-predict soil erosion for larger measured values. Although a lot of sediment can be generated in small rainfall event as fine-grained soil of the high water repellency was exposed excessively right after wildfire, this under-prediction was shown that those models have a limit to estimate the weighted factors by wildfire.

A Commissioning of 3D RTP System for Photon Beams

  • Kang, Wee-Saing
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.119-120
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    • 2002
  • The aim is to urge the need of elaborate commissioning of 3D RTP system from the firsthand experience. A 3D RTP system requires so much data such as beam data and patient data. Most data of radiation beam are directly transferred from a 3D dose scanning system, and some other data are input by editing. In the process inputting parameters and/or data, no error should occur. For RTP system using algorithm-bas ed-on beam-modeling, careless beam-data processing could also cause the treatment error. Beam data of 3 different qualities of photon from two linear accelerators, patient data and calculated results were commissioned. For PDD, the doses by Clarkson, convolution, superposition and fast superposition methods at 10 cm for 10${\times}$10 cm field, 100 cm SSD were compared with the measured. An error in the SCD for one quality was input by the service engineer. Whole SCD defined by a physicist is SAD plus d$\sub$max/, the value was just SAD. That resulted in increase of MU by 100${\times}$((1_d$\sub$max//SAD)$^2$-1)%. For 10${\times}$10 cm open field, 1 m SSD and at 10 cm depth in uniform medium of relative electron density (RED) 1, PDDs for 4 algorithms of dose calculation, Clarkson, convolution, superposition and fast-superposition, were compared with the measured. The calculated PDD were similar to the measured. For 10${\times}$10 cm open field, 1 m SSD and at 10 cm depth with 5 cm thick inhomogeneity of RED 0.2 under 2 cm thick RED 1 medium, PDDs for 4 algorithms were compared. PDDs ranged from 72.2% to 77.0% for 4 MV X-ray and from 90.9% to 95.6% for 6 MV X-ray. PDDs were of maximum for convolution and of minimum for superposition. For 15${\times}$15 cm symmetric wedged field, wedge factor was not constant for calculation mode, even though same geometry. The reason is that their wedge factor is considering beam hardness and ray path. Their definition requires their users to change the concept of wedge factor. RTP user should elaborately review beam data and calculation algorithm in commissioning.

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Construction of an Underground Heat Exchanger for Pig Housing (양돈용 지열교환기의 개발)

  • ;;H. J. Heege
    • Journal of Animal Environmental Science
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    • v.1 no.2
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    • pp.125-136
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    • 1995
  • To use the earth heat for the pig housing, an underground heat exchanger has constructed in depth of 2.5m and 20m length. The temperature of the outlet air was max. 8 kelvin higher than that of inlet air in winter season. In spite of the -7$^{\circ}C$ outside temperature, it could keep the air temperature from the earth tube above zero degree. The heating performance was maximum in value of 3.25Wh/㎥ and average of 1.75Wh/㎥ by the airflow volume of 340㎥/h. The slope of relative humidity from outlet air has shown gentler than that of inlet air. By using the underground heat exchanger, it would be possible to prepare an relatively uniform relative humidity in the swine stalls. The temperatures on the earth, where PVC pipes are buried, have shown 10~12$^{\circ}C$ on March. This can reduce the difference between day and night temperature during this season by using the underground heat exchanger.

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