• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.3
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• pp.1191-1196
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• 2013

Among Gram-negative pathogens in Korea, the incidence of resistance to third generation cephalosporins is becoming an ever-increasing problem. The production of extended-spectrum ${\beta}$-lactamase (ESBL) is the main mechanism of bacterial resistance to a third-generation cephalosporins and monobactams. Accurate identification of the ESBL genes are necessary for surveillance and epidemiological studies of the mode of transmission in the hospital. This study was conducted to detect the genes encoding ESBL of 46 K. penumoniae isolated from Daejeon, Chungnam and Chungbuk regional university hospitals from February to August in 2012. The phenotypes of the isolated specimens were examined according to the combination disc test (CDT) by the Clinical and Laboratory Standards Institute (CLSI). Forty two ESBL producing K. penumoniae isolates could be detected using ceftazidime (CAZ) discs with and without clavulanate (CLA). By CDT, 42 K. pneumoniae strains were confirmed to be ESBL strains. Genotyping was performed by multiplex PCR with type-specific primers. By PCR analysis, TEM gene in 46 strains, SHV gene in 37 strains and CTX-M genes in 14 strains were identified. Ten isolates did carry genes encoding ESBLs of all types TEM, SHV and CTX-M. The multiplex polymerase chain reaction (PCR) analysis was better to detect and differentiate ESBL producing K. penumoniae strains in clinical isolates.

• Journal of Life Science
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• v.22 no.4
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• pp.552-558
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• 2012

Severe acute respiratory syndrome (SARS) is a severe respiratory infectious disease caused by a novel human coronavirus, SARS-CoV. The 3CL protease is a key enzyme in the proteolytic processing of replicase polyprotein precursors, pp1a and pp1ab, which mediate all the functions required for viral genomic replication and transcription. Therefore, this enzyme is a target for the development of chemotherapeutic agents against SARS. A large quantity of active SARS-3CL protease is required for development of anti-SARS agents. Here we have constructed overexpression vector for the production of the SARS-3CL protease. The gene encoding SARS-3CL protease was amplified using polymerase chain reaction and cloned into the pET29a expression vector, resulting in pET29a/SARS-3CLP. Recombinant SARS-3CL protease was successfully synthesized by the dialysis mode of the cell-free protein expression system, and purified by three-step fast protein liquid chromatography using HighQ and MonoP column chromatographies and Sephacryl S-300 gel filtration. In addition, the produced SARS-3CL protease was found to be an active mature form. This study provides efficient methods not only for the development of anti-SARS materials from natural sources, but also for the study of basic properties of the SARS-3CL protease.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.4
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• pp.457-463
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• 2014

Ultrasound measurements of backfat thickness (UBF), longissimus muscle area (ULMA) and marbling score (UMS) and carcass measurements of carcass weight (CW), backfat thickness (BF), longissimus muscle area (LMA), and marbling score (MS) on 7,044 Hanwoo steers were analyzed to estimate genetic parameters. Data from Hanwoo steers that were raised, finished in Hoengseong-gun, Gangwon-do (province) and shipped to slaughter houses during the period from October 2010 to April 2013 were evaluated. Ultrasound measurements were taken at approximately three months before slaughter by an experienced operator using a B-mode real-time ultrasound device (HS-2000, FHK Co. Ltd., Tokyo, Japan) with a 3.5 MHz linear probe. Ultrasound scanning was on the left side between 13th rib and the first lumbar vertebrae. All slaughtering processes and carcass evaluations were performed in accordance with the guidelines of beef grading system of Korea. To estimate genetic parameters, multiple trait animal models were applied. Fixed effects included in the models were: the effects of farm, contemporary group effects (year-season at the time of ultrasound scanning in the models for UBF, ULMA, and UMS, and year-season at slaughter in the models for CW, BF, LMA, and MS), the effects of ultrasound technicians as class variables and the effects of the age in days at ultrasound scanning or at slaughtering as linear covariates, respectively for ultrasound and carcass measures. Heritability estimates obtained from our analyses were 0.37 for UBF, 0.13 for ULMA, 0.27 for UMS, 0.44 for CW, 0.33 for BF, 0.36 for LMA and 0.54 MS, respectively. Genetic correlations were strongly positive between corresponding traits of ultrasound and carcass measures. Genetic correlation coefficient between UBF and BF estimate was 0.938, between ULMA and LMA was 0.767 and between UMS and MS was 0.925. These results suggest that ultrasound measurement traits are genetically similar to carcass measurement traits.

• Microbiology and Biotechnology Letters
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• v.42 no.2
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• pp.106-113
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• 2014

For the development of high value consumables utilizing jujube and grape, we investigated the biological activities of a variety of existing fermentation products of jujube and grape. The results revealed that ethanol fermentation products of jujube and grape had a higher antioxidative activity than acetic acid fermentation products. In addition, the ethanol fermentation products of jujube (JEF) had the highest antioxidative activity, with it being greater than that of the ethanol fermentation products of grape (GEF), the acetic acid fermentation products of jujube (JAF) and the acetic acid fermentation products of grape (GAF). As regards tests on whitening effects, JEF exhibited the highest tyrosinase inhibition effects amongst the test groups. However, when immunofluorecence was employed, JAF was seen to inhibit the expression of proteins related to the whitening effect. In the lipopolysaccharide-stimulated mode peritoneal macrophage model, all tested groups of fermentation products (JEF, GEF, JAF and GAF) suppressed nitric oxide production dose-dependently, with ethanol fermentation products demonstrating a higher nitric oxide expression inhibition effect than acetic acid fermentation products. When subjected to antibacterial activity tests, GAF exhibited antibacterial activity against all tested strains except Propionibacterium acnes. Both GAF and JEF revealed high antibacterial activity against Escherichia coli.

• Journal of Energy Engineering
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• v.23 no.3
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• pp.186-190
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• 2014

Nuclear Power Plants require the control ability of chemical condition (pH) because pH control during transient accident such as LOCA makes an able the fission product removal capability to be maintained, stress corrosion cracking of stainless steel equipment to be prevented and the production of hydrogen by aluminum and zinc to be minimized. An NPP is designed to control the pH of containment spray and sump coolant using the spray additives 30% NaOH in the event of loss of coolant accident. In this paper, the pH of sump coolant of an NPP during LOCA was analyzed and the fission products removal constant and decontamination factor were calculated according to Standard Review Plan 6.5.2 related to spray chemical conditions of pH. The calculated pH value of recirculation mode using the computer code corresponds to 8.09~9.67, which meets the chemical environment regulation requirements. The fission product removal capability caused by containment spray system is performed to provide input to radiation analysis.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.16 no.5
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• pp.189-203
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• 2021

Environmental changes, which are strongly requiring technological cooperation, such as technological development and strategic alliances according to industrial ecosystem change, have a significant impact on not only product quality but also services. Thus, there is a need for 'servitization' that can satisfy the needs of customers and the ecosystem of businesses through the convergence of manufacturing companies and services. This study uses the Expectation-Confirmation Model to examine the impact on user satisfaction and Continuance Usage Intention. Research(Study) was conducted on users who are using high-tech-based cars. For this, we used Expectation Confirmation(match expections for the user's pre-and post-use relationships), Perceived Ease, Perceived Usefulness, User Satisfaction and Habitual Use and using Continuance Usage Intention as a dependent variable. Their causation was examined with the spss 26.0 and smartpls 2.0 statistical programs. As implications of this study, Expectation Confirmation has been shown to have significant positive effects on Perceived Ease, Perceived Usefulness, User Satisfaction and Habitual Use. For this results, the expectations before and after the use of high-tech cars lead to improved daily lives convenience and(or) work efficiency, leading to user satisfaction and further Continuance Usage Intention. Motors consider it important to pursue the work improvements that consumers want and use it useful in daily lives in the production and sale of high-tech cars. It is expected that it will create natural habits for services that users are satisfied with, and that these habits will affect the continuous growth and understanding of the trend of change.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.4
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• pp.601-609
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• 2022

Jack-up drilling rigs are mobile offshore platforms widely used in the offshore oil and gas exploration industry. These are independent, three-legged, self-elevating units with a cantilevered drilling facility for drilling and production. A typical jack-up rig includes a triangular hull, a tower derrick, a cantilever, a jackcase, living quarters and legs which comprise three-chord, open-truss, X-braced structure with a spudcan. Generally, jack-up rigs can only operate in water depths ranging from 130m to 170m. Recently, there has been an increasing demand for jack-up rigs for operating at deeper water levels and harsher environmental conditions such as waves, currents and wind loads. All static and dynamic loads are supported through legs in the jack-up mode. The most important issue by society is to secure the safety of the leg structure against collision that causes large instantaneous impact energy. In this study, nonlinear FE -analysis and verification of the requirement against collision for 35MJ recommended by DNV was performed using LS-Dyna software. The colliding ship used a 7,500ton of shore supply vessel, and five scenarios of collisions were selected. From the results, all conditions do not satisfy the class requirement of 35MJ. The loading conditions associated with chord collision are reasonable collision energy of 15M and brace collisions are 6MJ. Therefore, it can be confirmed that the identical collision criteria by DNV need to be modified based on collision scenarios and colliding members.

• Transactions of the KSME C: Technology and Education
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• v.3 no.3
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• pp.165-173
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• 2015

This paper presents numerical results of static structural stability analysis in development of a vertical transfer device of a PRT(Personal Rapid Transit) vehicle. The vertical transfer of a fully occupied vehicle operating on a road network is the first attempt, which is expected to contribute to overcome the limitations of conventional 2-dimensional operation mode. In particular, the vertical transfer apparatus designed based on vertical circulating conveyors is capable of continuous transfer without time delay so that it enables to accommodate a high traffic density. This system has been frequently used in a logistics field; however, it is essential to assess a structural integrity because an external force by a vehicle weight is exerted on the conveyors in the form of a concentrated load unlike a conventional logistic transport. In this study, prior to the production process, the structural performance of the pilot design in an early stage is numerically evaluated using the commercial finite element method (FEM) solver (i.e., $Ansys^{(R)}$).

• Culinary science and hospitality research
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• v.17 no.5
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• pp.74-91
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• 2011

The purpose of this study is to investigate the efficiency of a government-supported project developed to improve the quality and hygiene levels of food, service, and restaurant facilities for foreign customers in Chungnam province. Data was collected from 53 restaurant owners and restaurant managers. This study empirically analyzes and presents a mode with key factors influencing transfer of training and training satisfaction. Multiple regression analysis methods were used to test the relationships among four factors for transfer of training, training satisfaction, and retraining intentions. There are three main findings. First, teaching methods and relevance factors had a significant effect on transfer, but instructor and education environment factors did not. Second, the teaching methods, instructor and education environment factors had a direct influence on training satisfaction, but the relevance factor didn't. Third, transfer and satisfaction both were influenced significantly by retraining. Based on the findings of this study, a trainer, designing training curriculum for a production environment, should emphasize the role of the trainer and focus on learning tools. Finally, a thorough learning design is needed for learner's satisfaction.

• Korean Journal of Remote Sensing
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• v.37 no.6_1
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• pp.1507-1515
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• 2021

If chlorophyll-a is estimated through ocean color remote sensing, it is able to understand the global distribution of phytoplankton and primary production. However, there are missing data in the ocean color observed from the satellites due to the clouds or weather conditions. In thisstudy, the missing data of the GOCI (Geostationary Ocean Color Imager) chlorophyll-a product wasreconstructed by using DINEOF (Data INterpolation Empirical Orthogonal Functions). DINEOF reconstructs the missing data based on spatio-temporal data, and the accuracy was cross-verified by removing a part of the GOCI chlorophyll-a image and comparing it with the reconstructed image. In the study area, the optimal EOF (Empirical Orthogonal Functions) mode for DINEOF wasin 10-13. The temporal and spatialreconstructed data reflected the increasing chlorophyll-a concentration in the afternoon, and the noise of outliers was filtered. Therefore, it is expected that DINEOF is useful to reconstruct the missing images, also it is considered that it is able to use as basic data for monitoring the ocean environment.