• 한국미생물·생명공학회지
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• 제28권5호
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• pp.291-297
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• 2000

A strain producing high levels of compaction was isolated from soil and identified as Penicillium sp. Y-8515 based on the morphological characteristics and ribosomal RNA sequence analysis. Optimization of several different carbon and nitrogen sources for the effective production of compaction was performed resulting in the medium compositions containing 5%(w/v) glucose, 1.0 % soybean meal, 0.5% yeast extract, 0.5%(NH$_4$)$_2$$SO_4$, 0.25%,$ NaH_2$$PO_4$, 0.25% $CaCO_3$. The fixed con-centration of glucose(5%, w/v) and relatively lower concentrations(less than 2.5%, w/v) of soybean meal stimu-lated the transformation of the growth morphology from filamentous to pellet form. Comparing to that by filamentous form, the production of compactin by pellet form increased up to 1.5 folds. In a fed-batch fermentation, continuous feeding of the mixture of glucose and nitrogen source at the ratio of 10:1 showed 3.5-fold more produc-tion yield of compaction comparing to the batch mode.

• Preventive Nutrition and Food Science
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• 제2권2호
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• pp.101-108
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• 1997

A lactic acid bacterium LAB3113, isolated from traditionally fermented Kimchi was found to produce bacteriocin whose activity was very specific toward lactobacilli and not effective against any other bacteria. Lactobacilli affected by the inhibitory substance included Lactobacillus delbrueckii-lactis, L. johnsonii, L. gsseri, and L. curvatus. Based upon biochemical and physiological characteristics, LAB3113 was classified as Lactococcus lactis, and its bacteriocin was named as lactococcin K3113. Lactococcus lactis. LAB3113 produced bacteriocin at th early stage of growth and the concentration of the bacteriocin did not decrease even after alt stationalry phase. Optimal temperature of bacteriocin production was $25^{\circ}C$ at the initial pH 7.0. Partially purified lactococcin K3113 was completely inactivated by protease, but not affected by lipase, lysozyme and RNase. The bacteriocin was very heat-stable even after autoclaving for 20 min. It was also stable in pH changes, an was not affected by th presence of solvents. lacotococcin K3113 appeared to act in bactericidal mode against L. delbrueckii-lactis ATCC4797. Molecular weight of lactococcin K3113 was calibrated as 10,500 dal by SDS-PAGE an activity staining. Lactococcus lactis LAB3113 had four residential plasmids of 3.7kb, 11.2kb, 15.5kb, and 48kh in molecular sizes. Plasmid profile analysis of mutant strain revealed that 15.5 kb plasmid was re-sponsible for the production of lactococcin K3113 and its immunity to the bacteriocin.

• Preventive Nutrition and Food Science
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• 제1권2호
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• pp.269-277
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• 1996

Bacteriocins are proteins produced by a heterogeneous group of bacteria that have a bactericidal effect on closely related organisms. Recently, bacteriocins from lactic acid bacteria and other food-related organisms have been the subject of much research because of their potential as food biopreservatives. Various modifications of agar plate diffusion assays are the most widely used methods even though the limitations of such assays are generally recognized. The ability to obtain a concentrated crude preparation on bacteriocin by optimizing production parameters greatly simplifies recovery of bacteriocin on subsequent purification steps. Some studies performed to optimize bacteriocins have been purified to homogeneity, and the amino acid sequences of many of these purified bacteriocins have been determined. Obtaining characterization data on purified bacteriocin will minimize the risk of overlapping of research and confusion on identification of these compounds. Several me-chanisms leading to cell death have been hypothesized. These include depletion of the proton motive force(PMF) across the cell membrane: RNase and/or DNase activity within the sensitive cell; and pore formation and lysis of sensitive cells at the cell membrane.

• International Journal of Advanced Culture Technology
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• 제2권2호
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• pp.19-24
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• 2014

Many people all over the world have been conducting a great deal of research to solve the problem of global warming since the great majority consider reduction of CO2 as the only solution for that. That is why the production and conservation of energy is thought to be highly crucial. while it is important to produce energy with the high efficiency, the efficient use of the energy is also important. This paper focused on the development of devices for the reducing electricity which is a primary energy source used in homes, shops, buildings, factories and so on. Also the objective of this paper is to develop the inference mechanism as the core component of the devices. Therefore, in this paper, we propose the inference algorithm for reducing the electricity consumption using SARIMA mode and present the feasibility of the procedure.

• 한국인쇄학회지
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• 제21권1호
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• pp.35-44
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• 2003

Because of computer developing, the digital image making the use of many a field of application with - web-above, electronic publishing. printing, dynamic image management and photo CD production etc., however many problems of save and management. The management image use of compression moth which don't have a affect on image, reduce file size. A study used sequential DCT0based mode and progressive DCT-based mode of JPEG(Joing Photographic Experts Group) compression method and Wavelet compression method. Therefore, the analog image and digital image was changed and applied by several stages according to compression rate. It made inquiries of the optimum compression rate that be compared quality transformation between original image and compressed image. As compression image was printing simply, the quality was studied by subjective valuation method, that was studied propriety and usefulness.

• International Journal of Naval Architecture and Ocean Engineering
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• 제11권1호
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• pp.11-21
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• 2019

In product design, the initial design stage is being increasingly emphasized because it significantly influences the successive product development and production stages. However, for larger and more complex products, it is very difficult to accurately predict product reliability in the initial design stage. Various design methodologies have been proposed to resolve this issue, but maintaining reliability while exploring design alternatives is yet to be achieved. Therefore, this paper proposes a methodology for conceptual design considering reliability issues that may arise in the successive detailed design stages. The methodology integrates the independency of axiomatic design and the hierarchical structure of failure mode, effects, and criticality analysis (FMECA), which is a technique widely used to analyze product reliability. We applied the proposed methodology to a liquefied natural gas fuel gas supply system to verify its effectiveness in the reliability improvement of the design process.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.297-302
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• 1999

Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67％ or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

• 한국컴퓨터정보학회논문지
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• 제21권1호
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• pp.39-47
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• 2016

In this paper, we propose vehicle SoC fault diagnosis platform using FlexRay protocol in order to detect the faults of semiconductor control chip even after vehicle production. Before FlexRay protocol by sending NFI (Null Frame Indicator) bit among the header segment and a specific identifier in the payload segment of FlexRay frame, this technique can be distinguishable from normal mode and test mode. By using this technique, it is possible to detect the faults such as performance degradation of vehicle network system caused by the aging or several problems of vehicle semiconductor chip. Also high reliability and safety of vehicle can be maintained by using structural test for vehicle SoC fault detection.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집
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• pp.418-421
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• 2001

The ceramic filters were developed using technology similar to that of quartz crystal and electro- mechanical filter. However, the key to this development involved the theoretical analysis of vibration modes and material improvements of piezoelectric ceramics. The primary application of ceramic filters has been for consumer-market use. Accordingly, a major emphasis has involved mass production technology, leading to low-priced devices. A typical ceramic filter includes monolithic resonators and capacitors packaged in unique configurations. Nakazawa developed a double-mode resonator as two acoustically coupled single resonators. And he developed 10.7MHz crystal filters using multi-energy trapping mode of thickness shear vibration. He succeeded in realizing a two-pole band pass filter response without external inductance by splitting a dot electrode to creak coupled symmetric and anti- symmetric vibration modes. Accordingly, the simulation for ceramic (inter were important. So that, this paper were investigated the pass frequency of filter on the electrode length and thickness of ceramic.

• 한국전기전자재료학회논문지
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• 제20권5호
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• pp.467-470
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• 2007

Application field of neutron beam is very broad including industry, medicine and science. But the research and development and use of neutron beam is restricted within in narrow limits in this country, because neutron beam facility is insufficient - a big research facility of nuclear reactor(HANARO) and some small industrial facilities which use radioisotope neutron source are available. This paper compare and investigate the results of experiment and numerical analysis of the discharge in the spherically convergent beam fusion device which were expected as a portable neutron source. The spherically convergent beam fusion device will offer stability in neutron production, possibility of movement for convenience, low construction cost and higher neutron flux than radioisotope neutron source. The star mode discharge which efficiently generate neutron, were observed at both results.