• Korean Journal of Veterinary Service
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• v.23 no.2
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• pp.137-142
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• 2000

In order to develop a good separation and simultaneous analysis of different sugar in an artificial mixed sugar solution, we analyzed 10 sugar components in an artificial mixed sugar solution composed of fructose, glucose, mannitol, sucrose, maltose, lactose, xylose, xylitol erythritol, and trehalose with using HPLC-ELSD or HPLC-RI. Separation and quantification by HPLC-ELSD was superior to those by HPLC-RI and detection sensitivity by HPLC-ELSD was higher then that by HPLC-RI as micorgram($\mu\textrm{g}$) level. 1. The units of minimal detectable limits were showed $\mu\textrm{g}$/$m\ell$ and ng/$m\ell$ by the HPLC-RI and HPLC-ELSD, respectively. 2. The condition of ELSD was drift tube temperature $82^{\circ}C$, $N_2$ gas flow rate 2.10 SLPM, and colum oven temperature $30^{\circ}C$, respectively. Isolation and recovery rates of single sugar from the multiple sugar solution was higher at the condition (time: flow rate: D.W.:ACN MeOH, min : $m\ell$/min:v:v:v) of linear gradient elution of mobile phase as 0 : 1.00 : 15 : 85 : 0.1 : 1.00 : 6 : 90 : 4, 17 : 1.00 : 10 : 70 : 20, 28 : 1.00 : 15 : 85 : 0 an 35 : 1.00 : 15 : 85 : 0, in order.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.222.1-222.1
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• 2003

This study was carried out to investigate the quality control of domestic and imported Puerariae Radix. It was analyzed by HPLC using ${\mu}$-Bondapak C$\sub$18/ column with 35% MeOH containing 1% CH$_3$3CO$_2$H system as the mobile phase at UV 254nm. Good linearity showed over the range of 10 to 200$\mu\textrm{g}$/$m\ell$(r$^2$=1) for Puerarin, and 0.5 to 10$\mu\textrm{g}$/mg(r$^2$=0.9999) for Daidzein. The average contents of Puerarin and Daidzein were 5.5${\pm}$1.2%(Domestic), 5.3${\pm}$0.7%(Imported), and 0.05${\pm}$0.02%(Domestic), 0.08${\pm}$0.02%(Imported).The average recovery rates of Puerarin and Daidzein were 101.8${\pm}$1.9% and 97.2${\pm}$0.7%, respectively.

• Journal of Korea Multimedia Society
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• v.24 no.8
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• pp.1138-1148
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• 2021

In this study, we developed a 'shared-use mobility'-mounted local particulate matter monitoring terminal technology to measure the actual particulate matter concentration around me. As a mobile terminal device in the form of an IoT sensor platform, it is designed to be separated into a control module and a sensor module to minimize interference between sensors and to consider the optimal observation position of each sensor. As a result of the field test, it was confirmed that particulate matter was locally different depending on time and space even within the same area. In addition, it was confirmed that the concentration of particulate matter in the relevant section differed by up to 100 times compared to the surrounding area due to specific sources of particulate matter such as unpaved roads. In addition, we positively reviewed the applicability of the service in the real-time metaverse environment using this result. Through technological advancement and application of multiple shared-use mobilities, we expect to be able to provide new services for practical smart city air environment monitoring, such as localized particulate matter information, air pollution event information, and identification of causes of particulate matter.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.431-434
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• 2005

The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.198-203
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• 2008

This study was conducted to compare the effects of feeding high protein and low energy milk replacer (HPR; CP 25%, ME 3.6 Mcal/kg DM) with low protein and high energy milk replacer (HPR; CP 21%, ME 4.2 Mcal/kg DM) on feed consumption, body weight (BW) gain, health and selected blood metabolites in Holstein calves during the pre-weaning period. At each feeding, each milk replacer (MR) was prepared by mixing 0.125 kg of dry MR in 1L of warm ($60^{\circ}C$) water. The calves were fed either HPR (n = 10) or HER (n = 10) using mobile plastic bottles fitted with soft rubber nipples. All calves received 1.8L diluted MR at each feeding 3 times daily during the first 4 weeks of age; feeding frequency was reduced to 2 times daily for the next 2 weeks of age and then to once daily during the last week of the experiment. Jugular blood was sampled in calves at day 7, 14, 21, 35 and 49 of age to enumerate selected metabolites. Daily MR, starter and hay intake during the pre-weaning period were similar in calves fed HPR and HER. Consumption of starter, MGH and total DM steadily increased with the age of calves. Final BW, daily BW gain and feed efficiency of calves were not affected by treatments. Serum glucose, cholesterol, creatinine were decreased (p<0.05) and blood urea N was increased (p<0.05) in calves fed HER or HPR as they grew older. Serum glucose, total protein and albumin concentrations in calves were not affected by treatments. Serum GPT and GOT concentrations were higher (p<0.05) in calves on HPR than on HER. Scouring score, days scoured, respiratory score, rectal temperature and general appearance were similar in calves fed HPR and HER. Poor general appearance (dullness and droopy ears) of calves fed either HPR or HER reflected nutritional insufficiency and stress. In conclusion, energy and protein concentrations in MR did not affect feed intake and BW gain in Holstein calves during the pre-weaning period. Poor general appearance and lower BW gain of calves compared to those reported in the literature for milk fed calves prompt a demand for further research to improve the daily nutrient supply to MR-fed calves.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.3
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• pp.277-288
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• 2013

3GPP LTE-Advanced (Third Generation Partnership Project Long Term Evolution-Advanced) as a next generation mobile communication standard introduced small base stations such as femto cells or pico cells, and D2D (Device-to-Device) communications between mobiles in the proximity in order to satisfy the needs of rapidly growing wireless data traffic. A diverse range of topics has been studied to solve various interference situations which may occur within a single cell. In particular, an introduction of a small base station along with D2D communication raises important issues of how to increase the channel capacity and frequency efficiency in HetNets (Heterogeneous Networks). To this end, we propose in this paper methods to manage the interference between the macro cell and other small cells in the HetNet to improve the frequency efficiency. The proposed CCN (Cluster Coordinator Node)-assisted ICI (Inter-Cell Interference) avoidance methods exploit the CCN to control the interference in HetNet comprising of an MeNB (Macro enhanced Node-B) and a large number of small cells. A CCN which is located at the center of a number of small cells serves to avoid the interference between macro cell and small cells. We propose methods of resource allocation to avoid ICI for user equipments within the CCN coverage, and evaluate their performance through system-level computer simulations.

• Analytical Science and Technology
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• v.8 no.2
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• pp.215-220
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• 1995

A simple, efficient and sensitive method was described for the simultaneous determination of catecholamine, indoleamine and related metabolites from the homogenates of the rat brain striatum by HPLC-ECD. The optimum mobile phase on a reverse phase $C_{18}$ column was 35mM sodium acetate buffer(included 10mM citric acid, 0.13mM $Na_4EDTA$, 0.58mM SOS, pH3-4):MeOH=85:15. The column temperature was $30^{\circ}C$. Dopamine(DA), 3, 4-dihydroxyphenyl acetic acid(DOPAC), homovanilic acid(HVA), 5-hydroxyindole acetic acid(5-HIAA), serotonin(5-HT) and noradrenaline(NA) could be separated and analysed to very small amount. The detection limits of this method were 2~10pg per injection for all components. The effects of age and sex of rat on the contents of the catecholamines and their metabolites in rat brain striatum were studied. The levels of DA and 5-HT contents of the 7 week old female rats were higher than those of the 7 week old male rats. As the age of rat increases, the contents of DOPAC increased significantly.

• Analytical Science and Technology
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• v.11 no.3
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• pp.179-188
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• 1998

The capacity factor of fifteen phenol derivatives was determined with respect to the concentration of ${\alpha}$- or ${\beta}$-cyclodextrin [CD], the type as well as the content of organic solvent in the mobile phase, and the temperature. The effect of the inclusion complex formation between solutes and ${\alpha}$- or ${\beta}$-cyclodextrin on their retention and selectivity has been investigated. The inclusion effect of ${\beta}$-cyclodextrin was the most effective in aqueous methanol, whereas only a poor effect was observed in aqueous tetrahydrofuran and aqueous acetonitrile. A plot of the reciprocal of the capacity factor against $[CD]_T$ gives a straight line and the dissociation constant, $K_D$ of the inclusion complex can be calculated from the slope. It was possible to estimate the $k_D$ values in 100% water from a linear plot of $pK_D$ vs. water content in the solution by extrapolation. The separation factor, ${\alpha}$, of two compounds has been found to be affected not only by the $[CD]_T$ but also by their $K_D$ values. Under optimum conditions, some mixtures of phenol derivatives were able to separate successfully.

• Food Science and Preservation
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• v.14 no.6
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• pp.722-726
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• 2007

Extracting and analytical conditions of curcumin, and removal of bitterness substance from Curcuma longa L. were investigated. Absorption maxima was shown to be 424 nm at methanol solvent. Optimal conditions for analysis of curcumin was Zorbax eclipse $C_{18}$ column ; mobile phase, 75% MeOH ; flow rate, 0.8 mL/min ; wave length, UV 424 nm. Curcumin component was analyzed to be the highest content in methanol extract. In all samples, extraction yield by heating was shown to be effective as compared to room temperature. Curcumin contents of methanol and ethanol extracts in extraction of room temperature were 14.4 and 14.2 times higher than that of water extract, respectively. Two hot solvent extracts has a high curcumin content being 150 mg% as compared to room temperature. Extracting time was an effective condition when it was extracted for 60 minutes for elevating the curcumin content of water and methanol extracts. Bitter substance (BS) was markedly decreased in water extract by heat treatment of above $80^{\circ}C$. BS was weak in $121^{\circ}C$ treatment than in room temperature and it was however strong in $100^{\circ}C$ treatment. RT and $70^{\circ}C$ heat treatment were not different in BS intensity.

• Journal of Institute of Control, Robotics and Systems
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• v.13 no.10
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• pp.1025-1031
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• 2007

GPS is widely used for positioning applications and attitude of a vehicle can be found also with multiple antennas. However, extremely weak signal level prevents GPS from indoor operation. DR with accelerometers and gyros and landmark based localization method used for indoor applications increase complexity and cost. In this paper, a simple but very efficient ultrasound based attitude determination system which determines both position and attitude in WSN is given. The range between transmitter and receivers are measured using the arrival time difference between ultrasound and RF signal. The 3 dimensional positions can be found using more than 3 range measurements. Furthermore, if more than 2 transmitters are used, the attitude can be determined using the baseline vectors obtained by differencing transmitter and receiver positions. The prototype system is implemented to evaluate the performance of the proposed method. In addition, an error analysis shows the relation between the attitude error and basel me length, quality of measurement and orientation of a vehicle. The static and dynamic experiments performed by micro mobile robot shows accurate position with less than 1.5cm error and attitude with less than 1 degree error can be obtained continuously with 20cm baseline. It is expected that these results can be adapted without modification to indoor applications such as home cleaning robot and autonomous wheelchair maneuvering.