• Title/Summary/Keyword: Mn12

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A bio-sensor SoC Platform Using Carbon Nanotube Sensor Arrays (CNT 배열을 이용한 bio-sensor SoC 설계)

  • Chung, In-Young
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.12
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    • pp.8-14
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    • 2008
  • A fully CMOS-integrated carbon nanotube (CNT) sensor array is proposed. After the sensor chip is fabricated in commercial CMOS process, the CNTs network is formed on the top of the fabricated sensor chip through the room-temperature post-CMOS processes. When the resistance of the CNT is changed by the chemical reaction, the read-out circuit in the chip measures the charging time of the $R_{CNT}$-Capacitor. finally the information of measured frequency is converted to a digital code. The CMOS sensor chip was fabricated by standard 0.18um technology and the size of the $8{\times}8$ sensor array is $2mm{\times}2mn$. We have carried out an experiment detecting the biochemical material, glutamate, using this sensor chip. From the experiment the CMOS sensor chip shows the feasibility of sensor for the simultaneous detection of the various target materials.

Effect of water scarcity during thermal-humidity exposure on the mineral footprint of sheep

  • Nejad, Jalil Ghassemi;Lee, Bae-Hun;Kim, Ji-Yung;Park, Kyu-Hyun;Kim, Won-Seob;Sung, Kyung-Il;Lee, Hong-Gu
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.12
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    • pp.1940-1947
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    • 2020
  • Objective: Combination of two stressors on alteration of mineral footprints in animals needs due attention to meet maximum production and welfare, particularly in grazing sheep. This study tested whether ewes (Ovis aries) exposed to water deprivation and thermal-humidity stressors had altered mineral footprints in their wool, serum, urine, and feces. Methods: Nine ewes (age = 3 years; mean body weight = 41±3.5 kg) were divided among a control group with free access to water, and treatment groups with water deprivation lasting either 2 h (2hWD) or 3 h (3hWD) after feeding. Using a 3×3 Latin square design, animals were assigned to treatment groups for three sampling periods of 21 days each (n = 9). Blood was collected by jugular venipuncture. Wool was collected at the end of periods 2 and 3. Metabolic crates designed with metal grated floors were used for urine and feces collection. We measured sodium (Na), magnesium (Mg), phosphorus (P), chloride (Cl), calcium (Ca), manganese (Mn), copper (Cu), iron (Fe), and zinc (Zn). Results: The wool mineral levels did not differ between the treatment groups, although K was marginally lower (p = 0.10) in the 2hWD group. The serum and urine mineral levels did not differ between the treatments (p>0.05). Fecal K was significantly lower in the 2hWD group than in the other groups (p≤0.05). Conclusion: In conclusion, water deprivation and thermal-humidity exposure altered the excretion of K, but not of other minerals, in the wool, urine, feces, or serum of ewes. Thus, no additional mineral supplementation is needed for water deprived ewes during thermalhumidity exposure.

Evaluation of the health status of preschool children stratified based on the weight-length index (WLI)

  • Shin, Kyung-Ok;Chung, Keun-Hee;Park, Hyun-Suh
    • Nutrition Research and Practice
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    • v.4 no.5
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    • pp.383-392
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    • 2010
  • This study was conducted to prepare basic materials and offer advice regarding dietary habits to prevent and cure childhood obesity by comparing and analyzing dietary habit, nutritional status, blood factors, and mineral contents of hair. All subjects were stratified by their weight-length index (WLI). According to the standard WLI values, 64.9% of children were within the normal value, 13.5% of children were underweight, and 21.6% of children were overweight and obese (WLI ${\geq}$ 110%). Overall, the score assessed dietary habit for all children was $21.32{\pm}2.55$ point (921 subjects), with 5.1% of children having excellent dietary habits and 3.1% having poor dietary habits. Additionally, 37.9% of underweight children, 37.6% of normal weight children, and 43.2% of overweight and obese children consumed higher amounts of protein than underweight children did (meat, fish, eggs, and soy products) (P < 0.05). Overweight and obese children consumed more fried foods than underweight or normal weight children (P < 0.05). Moreover, 38.0% of the children had hemoglobin levels of 12 g/dl, while 7.6% were anemic (11.1 g/dl). When a hematocrit level of 33% was taken as the standard, 11.0% of children were anemic. The plasma transferrin content was $263.76{\pm}54.52$ mg/dl in overweight and obese children. The mean values of Fe, Cu, Ca, Cr, Mn, Se, Na, K, Li, V, Co, and Mo were within the reference values, but the Zn concentrations of underweight, normal weight, and overweight and obese children were $67.97{\pm}28.51$ ppm, $70.09{\pm}30.81$ ppm, and $73.99{\pm}30.36$ ppm, respectively. The Zn concentration of overweight and obese children ($73.99{\pm}30.36$ ppm) was lower than that of the standard value (180~220 ppm). Therefore, a nutritional education program and new guidance for dietary pattern should be developed to reduce the number of underweight and overweight and obese children.

Using of Straw mat for Reduction on NPS pollution from Radish field (무밭에서 발생하는 비점오염원 저감을 위한 볏짚거적 사용)

  • Shin, Min-Hwan;Kang, Jong-Cheon;Park, Woon-Ji;Jang, Jeong-Ryeol;Choi, Joong-Dae
    • Proceedings of the Korea Water Resources Association Conference
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    • 2011.05a
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    • pp.283-287
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    • 2011
  • 농업비점오염원의 오염원 관리를 위해서는 오염물질의 발생과 운반에 직접적으로 영향을 미치는 농업수문학 기술에 바탕을 두고 우리나라의 집약농업의 특성을 정확히 반영할 수 있는 최적관리방법의 개발과 비점오염저감효과를 검증할 수 있는 현장실험이 수반되어야 한다. 따라서 본 연구에서는 지표피복재를 이용하여 밭의 토양침투를 증가시고 유속 및 유출량 감소를 통해 토양유실과 비점오염원을 저감하고자 하였다. 실험방법은 실제 영농과 동일하게 무를 재배한 뒤 볏짚거적을 이용하여 밭의 토양 표면을 피복하여 유량과 수질농도를 측정하였다. 연구기간 동안 총 3차례(2010년 9월 9~12일, 9월 21일, 10월 2~3일)의 강우가 발생하였고, 이때 발생한 강우량은 각 359.2 mm, 49 mm, 28.8 mm이였다. 각 시험포에서 볏짚거적으로 인해 저감된 유출량의 저감효율은 볏짚거적을 피복하지 않은 나지상태의 밭 보다 약 33∼75%의 저감효과가 있는 것으로 나타났다. 75%의 유출률 저감 효과가 가장 크게 나타난 강우사상은 30 mm 이하의 강우가 발생한 10월 2~3일의 강우사상에서 나타났다. 강우량과 강우강도는 28.8 mm와 1.92 mm/hr으로 나타났으며, 이때 발생한 유출량은 나지에서 $0.36\;m^3$, 볏짚거적을 피복한 시험포에서는 $0.08\;m^3$이 발생하였다. 나지상태와 볏짚거적을 피복한 시험포의 오염부하의 값을 비교한 결과 볏짚거적을 피복한 시험포에서 $BOD_5$ 항목은 64.3%, SS 80.8%, $COD_{Mn}$ 66.7%, DOC 80.2%, T-N 56.6% 그리고 T-P 56.1%의 오염부하 저감효과가 나타났다. 본 연구의 결과와 같이 지표를 볏짚거적으로 피복할 경우 시험포에서 유출시작 시간 지연효과와 강우에 의한 첨두유출량 및 유출량 감소 효과가 나타나는 것으로 나타났으며, 연구결과는 농업 비점오염원의 최적관리를 위한 과학적 근거자료 제공 및 기초자료로 활용할 수 있을 것으로 판단된다.

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Characteristics of Fe-Mn Mineralization in Ugii Nuur and Tamir Gol, Mongolia (몽골 우기누르와 타미르골의 철-망간 부존 특성)

  • Lee, Bum Han;Park, Gye Soon;Kim, In Joon;Lee, Gilljae;Heo, Chul-Ho;Koh, Sang-Mo
    • Journal of the Mineralogical Society of Korea
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    • v.25 no.4
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    • pp.313-322
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    • 2012
  • 몽골 우기누르 지역 철-망간 광상과 타미르골 지역 철 광상의 광체는 먼곤체지 층 내에 렌즈상으로 협재되는 특성을 갖는다. 이러한 광상은 캠브리아 기에서 실루리아기에 이르는 화산 기원의 퇴적형 광상인 타미르골-요루골 광상구에 해당된다. 우기누르 지역의 철-망간 광체와 타미르골 지역의 철광체는 주로 규암과 편암을 모암으로 하여 먼곤체지 층 내에 렌즈상으로 협재되어 있다. 우기누르지역의 편암이 주로 세리사이트 편암인 데 비해 타미르골 지역은 주로 백운모 편암이 나타나는 차이를 갖는다. 또한 우기누르 지역의 광석은 망간이10에서 12% 함유되나 타미르골 지역의 광석은 망간이 1% 이하로 함량이 낮은 특성을 갖는다. 우기누르 철 망간 광상의 철 광물은 주로 자철석, 적철석이 우세하게 나타나고 기타 철 산화물과 황철석이 미량으로 수반되어 나타나며, 망간 광물은 주로스페사틴, 버네사이트가 우세하게 나타나고 기타망간 산화물이 수반되어 나타난다. 타미르골 지역의 철 광석은 자철석이 우세하게 나타나고 적철석이 수반되며 황철석, 철 산화물, 탄산질 철 등이 미량으로 수반되어 나타난다. 우기누르 철-망간 광상에 대한 육상 자력탐사 결과 높은 자기 이상값을보이는 영역이 지표에서 확인된 광체의 방향과 같은 약 $N30^{\circ}W$ 방향으로 나타나며 지표에서 확인된 광체 이외에 지표에 드러나지 않은 부분에서도 연장되는 것이 확인되었다.

Studies on the effect of silicate on nutrients up take using radioisotopes in rice plant. (I) (RI를 이용한 규소시용이 수도의 영양필요흡수에 미치는 영향(I))

  • Choon-Johong Ro
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.15-20
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    • 1972
  • There are still many problems on the physiological role of silicon in rice plants, although, it has been reported that silicate slag is the most effective fertilizer for the improvement of Akiochi soils. This experiment is carried out to investigate the effect of silicate of the mineral nutrition uptake by rice plants, using Ca45, P32, C136, and Mn54. The obtained results are summerized as follows; 1. Contents of mineral nutrients except silicon were higher in the rice plant grown in minus Si-culture solution a than in plus Si-plot. 2. Transpiration amounts appeared to be greater in the low content plant of silicon. 3. The rate of translocation to shoot of each ion absorbed by roots depended on the rate of transpiration. 4. It seemed that only CI uptake was found small in minus Si-root. 5. Silicate fetilizer did not affect the solubility of mineral ions in soils.

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Purification and Characterization of Chitinolytic Enzymes Produced by Aeromonas sp. J-5003

  • Choi Yong Un;Kang Ji Hee;Lee Myung Suk;Lee Won Jae
    • Fisheries and Aquatic Sciences
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    • v.6 no.1
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    • pp.7-12
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    • 2003
  • Chitinase and chitobiase produced by Aeromonas sp. J-5003 were purified and characterized. The chitinase was purified to 19.4 folds by gel chromatography and ion-exchange chromatography with the overall yield of $2.2\%$ and the specific activity of 93.1 unit/mg. The purified enzyme showed a single band on SDS-PAGE with MW 54kDa. The optimum pH and temperature of the purified chitinase were 7.0 and $37^{\circ}C$, respectively, and this enzyme stable in the range of pH 6.0 to 10.0 below $37^{\circ}C$. $Mg^{2+},\;Ca^{2+}\;and\;Na^+$ slightly stimulated the chitinase activity. However, $Hg^{2+}\;and\;Fe^{3+}$ inhibited chitinase activity. The chitobiase was purified by Sephacryl HR-l00 gel chromatography and DEAE-Sephadex A-50 ion-exchange chromatography with 33.5 purification folds and $4.3\%$ yield. The purified enzyme showed a single band with MW 63 kDa. The optimum pH and temperature of the purified chitobiase were 7.0 and $37^{\circ}C$, respectively. And this enzyme was stable in the range of pH 6.0 to 9.0 and at the temperature below $37^{\circ}C$. The enzyme activity was increased by $Mn^{2+}$, but it was inhibited by $Ag^+$.

Isolation and Molecular Analysis of Methanol Oxidation Genes in an Obligate Methylotrophic Bacterium, Metheylobacillus sp. Strain SK-5

  • Choi, Hack-Sun;Kim, Jin-Kwon;Ahn, Yeong-Hee;Koh, Moon-Joo;Kim, Si-Wouk
    • Journal of Microbiology and Biotechnology
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    • v.12 no.5
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    • pp.819-825
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    • 2002
  • Methanol dehydrogenase (MDH) is a key enzyme in the process of methanol oxidation in methylotrophic bacteria. However, information on MDH genes from genus Methylobacillus is limited. In this study, a 6.5-kb HindIII DNA fragment of Methylobacillus sp. SK-5 chromosomal DNA was isolated from the genomic library of the strain by using a degenerate oligonucleotide probe that was designed based on JV-terminal amino acid sequence of the MDH $\alpha$ subunit purified from the strain. Molecular analysis of the fragment revealed four tightly clustered genes (mxaFJGI) involved in the methanol oxidation. The first and fourth genes were very similar to mxaF (77% identity for nucleotides an 78% identity for amino acids) and mxaF (67% Identity for nucleotides and 68% Identity for amino acids) genes, respectively, from Methylovorus sp. SSI. Genes mxaF and mxaI encode $\alpha$ and $\beta$ subunits of MDH, respectively. The two subunits were identified from purified MDH from Methylobacillus sp. SK-5. A dendrogram constructed by comparison of amino acid sequences of MDH u subunits suggests that MxaF from Methylobacillus sp. SK-5 belongs to a subfamily cluster of MDH u subunits from $\beta$-subgroup Proteobacteria. The subfamily cluster is separated from the other subfamily that consists of $\beta$- and $\gamma$-subgroup Proteobacteria. This study provided information on mn genes from a methylotrophic bacterium in $\beta$-subgroup Proteobacteria, which would aid to better develop a gene probe to detect one-carbon metabolizing bacteria.

Evaluation of treatment efficiencies of pollutants in daecheong lake juwon stream constructed wetlands (대청호 주원천 인공습지의 오염물질 정화효율 평가)

  • Kim, Tae-Hun;Sung, Ki-Eun;Ha, Duk-Ho;Kim, Dong-Hee;Heo, Soon-Uk;Choi, Chung-Sik
    • Journal of Korean Society of Water and Wastewater
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    • v.29 no.2
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    • pp.211-222
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    • 2015
  • This study focused on evaluating the efficiency of the removal of non-point source pollution by Daecheong Lake Juwon Stream constructed wetlands. The constructed wetland system is a surface flow type designed in the year 2007 for purifying eutrophic water of Daecheong Lake Juwon Stream. The value of conductivity, suspended solids(SS), chemical oxygen demand using a potassium permanganate($COD_{Mn}$), five-day biochemical oxygen demand($BOD_5$), total nitrogen(T-N), total phosphorous(T-P), and pH in inflow averaged 220.2, 2.46, 3.33, 1.34, 2.00, 0.04 mg/L and 7.24, respectively and in outflow averaged 227.9, 1.12, 3.34, 0.87, 1.16, 0.02 mg/L and 7.45, respectively. The average removal efficiency of constructed wetlands was 30 % for SS, 22 % for $BOD_5$, 45 % for T-N and 31 % for T-P. The removal rates of SS, $BOD_5$ and T-N in the spring, summer and autumn were higher than those in winter. The removal rate of T-P was not significant different in all seasons. The amounts of pollutants removal in the constructed wetlands were higher in the order of $3^{rd}$ < $2^{nd}$ < $1^{st}$ wetland for SS and T-P, $2^{nd}$ < $3^{rd}$ < $1^{st}$ wetland for $BOD_5$ and T-N. Therefore, our findings suggest that the constructed wetlands could well treat the eutrophic Daecheong Lake Juwon Stream waters.

Effects of Silk Fibroin on Oxygen radicals and Their Scavenger Enzymes in Brain of SD Rats (뇌조직의 활성산소 및 그 제거효소에 미치는 실크 피브로인의 영향)

  • 최진호;김대익;박수현;김정민;조원기;이광길;여주홍;이용우
    • Journal of Life Science
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    • v.10 no.4
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    • pp.340-346
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    • 2000
  • This study was designed to investigate the effects of silk fibroin(Mw 500) powder (SFP) on oxygen radicals and the scavenger enzymes in brain membranes of rats. Spragu-Dawley(SD) male rats(160${\pm}$10g) were fed basic diet(control group), and experimental diets(SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0g/kg BW/day for 6 weeks. Hydroxyl radical($.$OH) levels resulted in a decreases(6.6% and 9.7%, 2.8% and 11.9%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, but were significantly decreased in these membrances of SFP-5.0 group only. Superoxide radical (O2) levels were a slightly decreased (2.0% and 9.1%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide(LPO) levels were significantly decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (16.7% and 15.7%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 group compared with control group, but significantly difference between in brain mitochondria of these two groups could not be obtained. Mn-SOD activities were remarkably increased (11.2% and 24.2%, respectively) in mitochodria of SFP-2.5 and SFP-5.0 groups. CuZn-SOD activities were effectively increased (7.7% and 19.6%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups, but significant difference between control and SFP-2.5 groups could be not obtained. GSHPx activities were considerably increased (5.3% and 11.7%, respectively) in brain cytosol of SFP-2.0 and SFP-5.0 groups compared with control group. There results suggest that anti-aging effect of silk fibroin may play an effective learning and memory role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in brain membranes.

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